High-dose therapy (HDT) combined with autologous hematopoietic stem cell transplantation (AHSCT) is a standard treatment for relapsed or refractory aggressive lymphoma. However, the role of HDT/AHSCT in the first-line treatment of lymphoma remains controversial, and related issues about its application in different subtypes of lymphoma should be addressed. This article brief-ly reviewed the clinical progress in AHSCT for malignant lymphoma treatment.

AIM:To investigate the effect of microRNA-16 ( miR-16) on the proliferation, invasion and cyto-kine secretion of rheumatoid arthritis ( RA) synovial fibroblasts ( RASFs) from the RA patients.METHODS: miR-16 mimic and miR-16 inhibitor were synthesized, and then Transfected into RASFs isolated from RA patients with lipo-fectamine.MTT assay, Transwell chamber and flow cytometry were used to determine the effect of miR-16 on proliferation, invasion and apoptosis of RASFs.The expression of matrix metalloproteinase 3/13 ( MMP3/13) and interleukin 1β( IL-1β) was measured by RT-PCR and Western blotting.RESULTS: The proliferation and invasion of RASFs were signifi-cantly inhibited by miR-16 mimic.The result of flow cytometry demonstrated that miR-16 had no effect on apoptosis of RASFs.Furthermore, miR-16 down-regulated the expression of MMP3/13 and IL-1β.CONCLUSION:miR-16 plays an important role in the development of RA and may inhibit the proliferation and invasion of RASFs through down-regulating the expression of MMP3/13 and IL-1β.

Objective To investigate the treatment of threatened abortion progesterone maternal serum pro-gesterone -induced blocking factor (PIBF)levels and clinical significance,in order to be able to provide theoretical references of progesterone treatment of threatened abortion.Methods Threatened abortion women (study group)and non -threatened abortion women (control group),50 patients in the observation group given progesterone therapy, enzyme -linked immunosorbent assay (ELISA)was used to detect serum progesterone and PIBF levels of the obser-vation group before treatment and two weeks later,the control group was selected and named after two weeks.Results Observation group after treatment 47 patients progesterone tocolysis successful,success rate was 94.00%,tocolysis bleeding stop time 8.42d,abdominal pain time 11.32d.PIBF of the observation group was (313.52 ±90.43)mmol/L, which was lower than that of the control group (398.47 ±104.25)mmol/L,the difference was statistically significant (t =10.90,P <0.05).The progesterone of the observation group after 2 weeks of treatment was (92.35 ±20.85)mmol/L, which was higher than that before treatment (74.49 ±14.22)mmol/L,compared with progesterone levels of the con-trol group at the same time (83.26 ±20.14)mmol/L and (78.05 ±15.38)mmol/L,the difference was statistically significant (t =7.96,8.11,P <0.05),PIBF after 2 weeks of treatment in the observation group was (510.73 ± 87.49)mmol/L,which was higher than that of the control group at the same time (498.26 ±69.88)mmol/L,the difference was statistically significant (t =4.35,P <0.05).Follow -up observation group miscarriage successful term delivery rate 95.74% of pregnant women,childbirth fetal malformations seen in the control group of pregnant women at term birth rate was 94.00%,no delivery fetal malformation,the difference of full -term delivery rate was not statis-tically significant (P >0.05).Conclusion Reduced PIBF during early pregnancy may be one of the reasons of threatened abortion,the use of progesterone therapy can increase PIBF expression,possibly through this pathway plays a role in tocolysis,so when using progesterone tocolysis monitoring PIBF levels helps evaluate the efficacy of tocolysis.

This article introduces the basic principles of finite element analysis in biomechanics, focusing on the basic principles of a variety of finite element analysis software, and their respective characteristics. In addition, it also de scribes the basic stress analysis of UGNX6.0 NASTRAN analysis for artificial knee process, i. e. the choice of the type, material definition, the set of constants, finite element mesh division and the finite element results of the analysis. Finite element analysis and evaluation of the design of personalized artificial knee were carried out, so that the rationality of the geometric design of the structure of the experimental design of artificial knee has been verified.
Biomechanical Phenomena ; Bionics ; Finite Element Analysis ; Humans ; Knee Joint ; Knee Prosthesis ; Prosthesis Design ; Stress, Mechanical

Female ; Fertility ; Humans ; Nutritional Status

Objective To investigate the characteristics of pathogens and bacterial susceptibility in the pa-tients with ventilator-associated pneumonia (VAP). Methods The clinical characteristics of aetiological germ and bacterial susceptibility in 20 patients with VAP who were treated in the hospital department of respiratory medicine were analyzed. Results 20 patients with bronchial secretions cultured bacteria was 42. It showed that the isolating rate of gram-negative bacilli(GNB), gram-positlve cocci and fungus was 71.4%, 16.7% and 11.9% respeotively. Conclusions The main bacterium in patients with VAP was GNB,which exhibited higher resistance to common anti-biotics. More attention should be paid to the cultivation of aetiological bacteria and selection of antibiotics in the treat-ment of VAP.

Objective To observe the influence of rehabilitative exercise on COPD patients in revive period in the pulmonary function, six-mlnute walking distance and activity of diaphragm as well as the number of acute attack in the last year. Methods Total 50 cases of COPD patients in revive period were randomly divided into comparison group and rehabilitative exercise group, each 25 cases. The former recaived general internal medicine for the treat-ment. In the latter on the basis of general medical treatment in patients with the disease in accordance with the formu-lation of rehabilitation programs,account of patients and their families in the light of the implementation,and stay pa-tient contact to monitor the implementation and follow-up. Results Rehabilitation treatment group compared with control group,rehabilitative exercise had mere notable effects in the pulmonary function,six-minute walking distance and activity of diaphragm of the COPD patients in revive period, the number of acute attack was significantly lower than that of the comparison group. Conclusion Rehabilitative exercise has comprehensive effects in the respect of improving life quality of patients, reducing times of hospitalization and seeking medical advices.

The treatment of Graves's hyperthyroidism with antithyroid drugs can attain the optimal outcome due to intact thyroid tissue "after treatment. The rational choice of indication, related factors for improving the prognosis of disease and evaluation of withdrawal index of antithyroid drugs are briefly discussed in the review.

Because of the particularity in causes, mecha-nisms and clinical performances, injury in choledocho-pancreatico-duodenal junction is usually doomed with a delayed diagnosis, often leading to a poor prognosis. The early manifestations of bile duct perforation include peritoneal swelling caused by detained water after trans-T-tube injection, blue staining of the field of operation and contrast medium leaking outside the bile duct system, peritoneal or abdominal gas accumulation, pneu-mothorax or subcutaneous emphysema after endoscopic sphincte-rotomy (EST) or endoscopic retrograde cholangiopancreatogra-phy (ERCP). Postoperative high fever, abdomical pain radia-ting to right side back and waist, fluid accumulation in the right iliac fossa or around the right kidney are the associated evidences. If the perforation is discovered during the operation, it should be sutured and choledocal T-tube drainage should be performed. If the perforation is not discovered during the opera-tion, biliointestinal bypass should be constructed. The injuries resulted from ERCP or EST procedures should be treated accord-ing to the detailed situation. Conservative treatment can be given to those who are in relatively stable status. If the condition of the patients deteriorated, timely conversion to laparotomy is needed. For patients with delayed diagnosis, thorough drainage of the region, separation of bile and pancreatic juice, duodenal diver-ticularization and jejunostomy should be considered. The key point in preventing the injury in choledocho-pancreatico-duode-hal junction lies on full knowledge of the anatomy of the region, delicate practice without forceful exploration and detailed exami-nation after the operation to avoid missing diagnosis.

There is a pressing need for new antibacterial agents due to the development of drug-resistant pathogens. Unfortunately drug development is a difficult and complicated process. The traditional approach in searching for a right dose is quite empirical, both costly and time-consuming. To enhance the ability to predict the likelihood of success for lead compound selection, in vitro pharmacodynamic and in vivo animal infection models are now extensively used. The value of these pre-clinical experiments, combined with mathematical modeling, helps to identify a pharmacokinetic (PK)-pharmacodynamic (PD) exposure measure which best predicts the therapeutic efficacy, and to quantify the magnitude of this index required for in vivo efficacy. PK-PD target attainment analyses using Monte Carlo simulation to integrate interpatient variability in drug exposure (PK), drug potency (MIC), and in vivo exposure targets that are predictive of positive therapeutic outcomes are influencing antibacterial drug development for proof of concept, for dose and dosing interval selection, for determining susceptibility breakpoints, and for evaluating the clinical meaning of antibacterial resistance. In this article, the key concepts of antibacterial PK-PD and model based antibacterial drug development strategy and process are critically reviewed.