AIM:To investigate the effect of microRNA-16 ( miR-16) on the proliferation, invasion and cyto-kine secretion of rheumatoid arthritis ( RA) synovial fibroblasts ( RASFs) from the RA patients.METHODS: miR-16 mimic and miR-16 inhibitor were synthesized, and then Transfected into RASFs isolated from RA patients with lipo-fectamine.MTT assay, Transwell chamber and flow cytometry were used to determine the effect of miR-16 on proliferation, invasion and apoptosis of RASFs.The expression of matrix metalloproteinase 3/13 ( MMP3/13) and interleukin 1β( IL-1β) was measured by RT-PCR and Western blotting.RESULTS: The proliferation and invasion of RASFs were signifi-cantly inhibited by miR-16 mimic.The result of flow cytometry demonstrated that miR-16 had no effect on apoptosis of RASFs.Furthermore, miR-16 down-regulated the expression of MMP3/13 and IL-1β.CONCLUSION:miR-16 plays an important role in the development of RA and may inhibit the proliferation and invasion of RASFs through down-regulating the expression of MMP3/13 and IL-1β.

High-dose therapy (HDT) combined with autologous hematopoietic stem cell transplantation (AHSCT) is a standard treatment for relapsed or refractory aggressive lymphoma. However, the role of HDT/AHSCT in the first-line treatment of lymphoma remains controversial, and related issues about its application in different subtypes of lymphoma should be addressed. This article brief-ly reviewed the clinical progress in AHSCT for malignant lymphoma treatment.

Objective To investigate the treatment of threatened abortion progesterone maternal serum pro-gesterone -induced blocking factor (PIBF)levels and clinical significance,in order to be able to provide theoretical references of progesterone treatment of threatened abortion.Methods Threatened abortion women (study group)and non -threatened abortion women (control group),50 patients in the observation group given progesterone therapy, enzyme -linked immunosorbent assay (ELISA)was used to detect serum progesterone and PIBF levels of the obser-vation group before treatment and two weeks later,the control group was selected and named after two weeks.Results Observation group after treatment 47 patients progesterone tocolysis successful,success rate was 94.00%,tocolysis bleeding stop time 8.42d,abdominal pain time 11.32d.PIBF of the observation group was (313.52 ±90.43)mmol/L, which was lower than that of the control group (398.47 ±104.25)mmol/L,the difference was statistically significant (t =10.90,P <0.05).The progesterone of the observation group after 2 weeks of treatment was (92.35 ±20.85)mmol/L, which was higher than that before treatment (74.49 ±14.22)mmol/L,compared with progesterone levels of the con-trol group at the same time (83.26 ±20.14)mmol/L and (78.05 ±15.38)mmol/L,the difference was statistically significant (t =7.96,8.11,P <0.05),PIBF after 2 weeks of treatment in the observation group was (510.73 ± 87.49)mmol/L,which was higher than that of the control group at the same time (498.26 ±69.88)mmol/L,the difference was statistically significant (t =4.35,P <0.05).Follow -up observation group miscarriage successful term delivery rate 95.74% of pregnant women,childbirth fetal malformations seen in the control group of pregnant women at term birth rate was 94.00%,no delivery fetal malformation,the difference of full -term delivery rate was not statis-tically significant (P >0.05).Conclusion Reduced PIBF during early pregnancy may be one of the reasons of threatened abortion,the use of progesterone therapy can increase PIBF expression,possibly through this pathway plays a role in tocolysis,so when using progesterone tocolysis monitoring PIBF levels helps evaluate the efficacy of tocolysis.

There is a pressing need for new antibacterial agents due to the development of drug-resistant pathogens. Unfortunately drug development is a difficult and complicated process. The traditional approach in searching for a right dose is quite empirical, both costly and time-consuming. To enhance the ability to predict the likelihood of success for lead compound selection, in vitro pharmacodynamic and in vivo animal infection models are now extensively used. The value of these pre-clinical experiments, combined with mathematical modeling, helps to identify a pharmacokinetic (PK)-pharmacodynamic (PD) exposure measure which best predicts the therapeutic efficacy, and to quantify the magnitude of this index required for in vivo efficacy. PK-PD target attainment analyses using Monte Carlo simulation to integrate interpatient variability in drug exposure (PK), drug potency (MIC), and in vivo exposure targets that are predictive of positive therapeutic outcomes are influencing antibacterial drug development for proof of concept, for dose and dosing interval selection, for determining susceptibility breakpoints, and for evaluating the clinical meaning of antibacterial resistance. In this article, the key concepts of antibacterial PK-PD and model based antibacterial drug development strategy and process are critically reviewed.

Objective To explore the clinical effects of recipe of Jiangtanghuoxue in the treatment of diabetic cerebral infarction and recovery of cognitive function .Methods 54 patients with diabetic cerebral infarction were se-lected and divided into control group and treatment group evenly and randomly .Control group received regular treat-ment,while treatment group received additional treatment of recipe of Jiangtanghuoxue .Total efficacy,level of HbA1c were detected ,MMSE and MoCA were used to test the cognitive function .Results Total efficacy in the treatment group(88.89%) was significantly higher than 70.57% of the control group (χ2 =4.358,P<0.05);The level of HbA1c was significantly lower in treatment group than that in the control group (P<0.05);score of MMSE and MoCA improved significantly after treatment for all patients(P<0.05),but more significantly in the treatment group(P<0.05). Conclusion Recipe of Jiangtanghuoxue is suitable in the treatment of diabetic cerebral infarction ,improving cognitive function at the same time .

Since there is no specific symptoms after human have been infected by parvovirus,parvovirus was administered as a potential cancer therapeutics.Parvovirus was employed as an effective treatment in glioblastoma multiforme,Burkitt' s lymphoma,liver cancer,pancreatic carcinoma,breast cancer and colon cancer.Anti-tumor effects of parvovirus were approved to be associated with its oncolytic effects,non-structural protein 1 and immunoregulation.

Objective To investigate the influence of anticoagulant drug warfarin on chronic subdural hematoma (CSDH) and the role in CSDF recurrence.Methods 136 patients with CSDH hole drainage surgery were selected.The clinical data were analyzed and the patients were followed up.INR level and the correct time in patients only used VK or used VK + PCC were compared between the two groups before and after treatment.The history of trauma,the recurrence rate,time to recurrence in patients received warfarin therapy or not were compared.Results The time of anticoagulated indicators INR dropped to normal was (6.4 ± 4.9)h in VK + PCC group,which was significantly shorter than in the VK group(14.5 ±7.6)h(P ＜0.05).In patients not received warfarin preoperatively,52 cases(57％) had a history of traumatic brain injury.12 patients (27％) had the history of traumatic brain injury in patients received warfarin preoperatively.The proportion of patients with a history of traumatic brain injury in without warfarin therapy group was significantly higer than the anticoagulant therapy group (P ＜ 0.05).A total of 18 cases(13％) had postoperative recurrence,they were given the CSDH drilling drainage again.The recurrence rate be tween the warfarin anticoagulant group (18 ％) and non-anticoagulant group (11％) had no significant difference P ＞0.05).Time to relapse in patients with preoperative use of warfarin therapy and without anticoagulant therapy was (20.5 ±8.1)d,(24.4 ± 13.2)d (P＞0.05).Conclusion Warfarin increased the possibility of non-traumatic CSDH,VK + PCC shortened the corrective warfarin anticoagulation time required.

Objective To understand the status quo of related knowledge,attitude and practice of religious belief among undergraduate nursing students and provide reference for education on religious belief.Methods A self-filled questionnaire on religious belief was used randomly among undergraduate nursing students in 4 medical universities in Wuhan.Results Most nursing students were inferior to the cognitive degree of related knowledge on belief religious,which was greatly related to the grade,nationality,parents’ religious belief and family residence factors.The notion of nursing students towards religious belief policy was nebulous.Most students could cognize the relationship between religion and science correctly.The university had minor effect on students’ religion view.Students contacted religious belief with various channels and the role of the network could not be underestimated.Undergraduate nursing students understood and pardoned their religious belief and other students’.Their religious belief was not the mainstream of current belief.The Buddhist belief of religion was in the first place.The majority of students went to religious activities not for the belief.Conclusions We should face the reality,examine rationally at this society and strengthen the social,school,family and individual guidance on religion together with related education coustruction.

Scholars at home and abroad have proved that the unbalance between CD4 + CD25 + Foxp3 +regulatory T cells (Treg cells) and Th17 cells relates to the pathogenesis of hematopathy.Patients with tumor blood diseases,such as leukemia and lymphoma,generally show raised levels of Treg cells and decreased levels of Th17 cells.The pathogenesis of disease may involve in Treg cells inducing enhanced immune suppression and Th17 cells mediating immune deficiency.On the contrary,cases with non-neoplastic blood diseases,such as AA,HSP,ITP and so on,trend to have lower Treg cells and higher Th17 cells.The pathogenesis of non-neoplastic blood disease may be connected with serious immune injury mediated by Th17 cells and weak suppression of immunity induced by Treg cells.Accordingly,the increasing ratio of Treg cells/Th17 cells may cause tumor blood diseases,but a decreasing one can promote non-neoplastic blood disease.

This article reviewed the research on evaluating benign and malignant thyroid nodules in recent years,and particularly focused on the oncogens and molecular markers.Their advantages and disadvantages in clinical use and application prospective were reviewed and analyzed.