AIM:To investigate the effect of microRNA-16 ( miR-16) on the proliferation, invasion and cyto-kine secretion of rheumatoid arthritis ( RA) synovial fibroblasts ( RASFs) from the RA patients.METHODS: miR-16 mimic and miR-16 inhibitor were synthesized, and then Transfected into RASFs isolated from RA patients with lipo-fectamine.MTT assay, Transwell chamber and flow cytometry were used to determine the effect of miR-16 on proliferation, invasion and apoptosis of RASFs.The expression of matrix metalloproteinase 3/13 ( MMP3/13) and interleukin 1β( IL-1β) was measured by RT-PCR and Western blotting.RESULTS: The proliferation and invasion of RASFs were signifi-cantly inhibited by miR-16 mimic.The result of flow cytometry demonstrated that miR-16 had no effect on apoptosis of RASFs.Furthermore, miR-16 down-regulated the expression of MMP3/13 and IL-1β.CONCLUSION:miR-16 plays an important role in the development of RA and may inhibit the proliferation and invasion of RASFs through down-regulating the expression of MMP3/13 and IL-1β.

High-dose therapy (HDT) combined with autologous hematopoietic stem cell transplantation (AHSCT) is a standard treatment for relapsed or refractory aggressive lymphoma. However, the role of HDT/AHSCT in the first-line treatment of lymphoma remains controversial, and related issues about its application in different subtypes of lymphoma should be addressed. This article brief-ly reviewed the clinical progress in AHSCT for malignant lymphoma treatment.

Objective To investigate the treatment of threatened abortion progesterone maternal serum pro-gesterone -induced blocking factor (PIBF)levels and clinical significance,in order to be able to provide theoretical references of progesterone treatment of threatened abortion.Methods Threatened abortion women (study group)and non -threatened abortion women (control group),50 patients in the observation group given progesterone therapy, enzyme -linked immunosorbent assay (ELISA)was used to detect serum progesterone and PIBF levels of the obser-vation group before treatment and two weeks later,the control group was selected and named after two weeks.Results Observation group after treatment 47 patients progesterone tocolysis successful,success rate was 94.00%,tocolysis bleeding stop time 8.42d,abdominal pain time 11.32d.PIBF of the observation group was (313.52 ±90.43)mmol/L, which was lower than that of the control group (398.47 ±104.25)mmol/L,the difference was statistically significant (t =10.90,P <0.05).The progesterone of the observation group after 2 weeks of treatment was (92.35 ±20.85)mmol/L, which was higher than that before treatment (74.49 ±14.22)mmol/L,compared with progesterone levels of the con-trol group at the same time (83.26 ±20.14)mmol/L and (78.05 ±15.38)mmol/L,the difference was statistically significant (t =7.96,8.11,P <0.05),PIBF after 2 weeks of treatment in the observation group was (510.73 ± 87.49)mmol/L,which was higher than that of the control group at the same time (498.26 ±69.88)mmol/L,the difference was statistically significant (t =4.35,P <0.05).Follow -up observation group miscarriage successful term delivery rate 95.74% of pregnant women,childbirth fetal malformations seen in the control group of pregnant women at term birth rate was 94.00%,no delivery fetal malformation,the difference of full -term delivery rate was not statis-tically significant (P >0.05).Conclusion Reduced PIBF during early pregnancy may be one of the reasons of threatened abortion,the use of progesterone therapy can increase PIBF expression,possibly through this pathway plays a role in tocolysis,so when using progesterone tocolysis monitoring PIBF levels helps evaluate the efficacy of tocolysis.

In recent years, a rapid development has been achieved in the clinical prevention of hepatocellular carcinoma recurrence after liver transplantation, especially in perioperative management, which are supported by the results of evidence-based studies.However, due to the complicated procedures and varied risk factors for liver transplantation, the prevention of postoperative carcinoma recurrence remains a challenge and a focus of research in liver transplantation.This study reviews the perioperative measures for the prevention of hepatocellular carcinoma recurrence after liver transplantation, aiming to shed some light on this aspect for graduates and clinicians of surgery.

Bicuspid aortic valve (BAV) is one of the most common congenital valvular diseases, which is often associated with aortic stenosis or insufficiency.Aortic disease is an important component of BAV, aortic root aneurysm is the most finding when it comes to the dilatation of any or all segments of the proximal aorta from the aortic root to the aortic arch.The choice of operation has a great influence on the prognosis of patients.This article discusses the anatomic basis, pathophysiology, surgical treatment and treatment effects of the bicuspid aortic valve with aortic root aneurysm.

Objective To analyze the relationship between the status of anxiety and depression and activity and participation for inpa-tients with spinal cord injury (SCI). Methods From January, 2014 to June, 2016, a total of 37 inpatients with SCI were assessed by Zung Self-rating Anxiety Scale (SAS), Zung Self-rating Depression Scale (SDS), and WHO Disability Assessment Schedule 2.0 (WHO-DAS 2.0) at discharge. Results The average scores of SDA were (0.5±0.18) and SAS were (41.15±14.7) respectively. The inpatients scored (1.43± 0.82) (cognition), (3.06±1.43) (mobility), (2.47±1.11) (self-care), (2.13±0.66) (getting around), (3.68±1.32) (life activity for household and work), and (3.57±0.84) (participation) in six domains of WHO-DAS 2.0 respectively. There were severe disabilities in mobility, life activity for household and work, and participation;mild disability in cognition;and moderate disabilities in self-care and getting around. There was positive correlation between depression and functions of mobility, life activity for household and work, and participation (P<0.05). Conclu-sion Depression influences the status of activity and participation for inpatients with SCI at recovery stage.

Reflux esophagitis is considered as a commonly-seen esophageal disease.In recent years,certain advances were archived internationally in researches about its pathogenesis,treatment,complications,etc.This article reviewed advances in researches of reflux esophagitis in the year 2016.

The effects of oligosaccharide and creatine (Cr) supplementation on glucose, lactic acid and urea nitrogen levels in blood and activity of serum creatine kinase (CK) were explored. Twenty CUBA male athletes were divided into 4 groups: group A (supplementation of Cr alone), group B (supplementation of oligosaccharide), group C (supplementation of oligosaccharide and Cr) and group D (placebo control group). By using orthogonal L4 table (2(3)), the experiment was performed. There were factors including oligosaccharide (carbohydrate, CHO), Cr and their correlation. Each factor had two levels: supplementation and no-supplementation. The results showed that the supplementation of CHO or Cr alone, combined supplementation of CHO and Cr could significantly reduce the glucose, urea nitrogen levels in blood and serum CK activity after competition in the athletes. Moreover, the effects of combined supplementation of CHO and Cr were more satisfactory. It was concluded that supplementation of CHO and Cr could promote the recovery of physical performance and athletic abilities after athletics in basketball athletes.
Athletes ; Basketball/*physiology ; Blood Glucose/*metabolism ; Blood Urea Nitrogen ; Creatine/*administration & dosage ; Creatine Kinase/*blood ; Dietary Supplements ; Oligosaccharides/*administration & dosage ; Young Adult

Objective To study the relationship between the serum HBV DNA level and theprogress of hepatitis B. MethodsHBV DNA was quantified by branch DNA signal amplification as-say(bDNA). 172 patients of different clinical types infected with HBV were investigated for HBVDNA. Results At the less than 0.7mmol/ml of HBV DNA level, 88.0% were acute patients,32.2% chronic,66.7% chronic with the fulminant hepatic failure, 19.4% cirrhotic, and 37.5% HCCpatients. The degree of fibrosis in the liver tissue is significantly higher in the group with more than 35years old. than in that with less than 35 years old ( P < 0.01 ). All chronic patients were analyzed,HBV DNA level is significantly higher in patients less than 35 years old than in patients more than 35years old ( P < 0.01 ). Conclusion This research demonstrated that existence and replication ofHBV were the main causes for the progress of hepatitis B.

Today's society, many people are plagued by tinnitus and deafness. Chinese medicine on tinnitus and deafness disease and its therapies and for-mulas have discussed quite wel . It is believed that according to the features of the organs, treating tinnitus and deafness from the five internal organs is much better .The virtual is mostly related to the heart, spleen and kidney, but the real mostly related to the heart, liver and lungs. In the treatment of tin-nitus and deafness from the perspective of the kidney, attention should be paid to nourishing the kidneys and strengthening the essence. Treating from the spleen, attaches great importance to the spleen and replenishing qi, clearing material. Treatment from the aspects of liver, the first is to make qi run unob-structed, to prevent generating phlegm and fire. From pulmonary vessels, great importance should be attached to the recovery of dispersing the descending lung. Treated from heart, benefiting qi and nourishing blood should be given much attention not only to make up for its deficiency, but also attach great importance to the qi, send a fire on its reduction. Sick of longer time, compatibility on the basis of syndrome differentiation and treatment to invigorate the flux pulse, aromatic resuscitation of Chinese herbal medicine shal be used.