OBJECTIVE: To introduce and analyze the present status of research on decomposed recipes of traditional Chinese medicine (TCM). METHODS: The relevant literature on the study of the decomposed recipes of TCM in the past five years was collected and analyzed. RESULTS AND CONCLUSION: Studies on the decomposed recipes of TCM have been made from the whole body level to the organ, cell and molecular research level. The pharmacological research in vitro is the main way and quantitative changes of the chemical ingredients have been measured before and after composition. It is very important to combine the modern science and technology in exploring the composing mechanisms of TCM remedies.

Animals ; Disease Models, Animal ; Endothelin-1 ; metabolism ; Male ; Nitric Oxide ; metabolism ; Paraquat ; poisoning ; Rats ; Rats, Sprague-Dawley

Objective To establish an HPLC method for the determination of plasma metformin hydrochloride and an HPLC/ESI-MS method for the determination of plasma glibenclamide in human. Methods The metformin plasma samples were added with acetonitrile to denature proteins and the supernatant was injected into the system directly by using EC 250/4.6 NUCLEOSIL 100-5 analytical column (4.6 mm?250 mm, 5 ?m) with a mobile phase of 0.03 mol/L (NH 4) H 2PO 4∶acetonitrile (75∶25, pH 7.0) at the flow rate of 1.0 ml/min and the detection wavelength of 240 nm. Using glipizide as a internal standard, the glibenclamide plasma samples were extracted with ethyl acetate and determined by LC-MS using a chromatographic column ORBAX SB300C 18 (2.1 mm?150 mm, 5 ?m) with a mobile phase of acetonitrile-0.1% ammonium acetate (55∶45, V/V) at the flow of 0.2 ml/min. Results The glibenclamide concentration had a good linear correlation in the range of 2-200 ng/ml (r=0.999 8) while the metformin concentration in the range of 6.25-4 000 ng/ml (r= 0.999 9 ). Both the inter-day and intra-day RSD of metformin and glibenclamide were lower than 5%. Conclusion The assays have been proved to be sensitive, accurate and convenient and can be used in the study of human pharmacokinetics of glibenclamide and metformin hydrochloride.

Diagnostic Errors ; Humans ; Inflammation ; diagnosis ; etiology ; Laryngeal Neoplasms ; surgery ; Male ; Middle Aged ; Papilloma ; surgery ; Pneumonia ; diagnosis ; Postoperative Complications ; diagnosis

Bacterial Infections ; microbiology ; therapy ; Humans ; Integrative Medicine ; Male ; Microbial Sensitivity Tests ; Middle Aged ; beta-Lactamases ; isolation & purification

Objective Using Hitachi 7170 external quality assessment return target value to evaluate the accuracy of 10 items after Regression calibration of the Vistros 350 dry-type Biochemical Analyzer.Methods The same quality control samples were separately tested on two instruments,and results were reported to the clinical National Center for Clinical Laborato-ries.Substituted the external quality assessment return target value result from the National Center for Clinical Laboratories by using Vitros350 into regression calibration equation,then the getting data were compared with the external quality assess-ment return target value obtained from Hitachi 7170,and the deviation analysis was processed.The total error range from the America Clinical Laboratory Amended Bill was used as the standard.For the results within the reference range,error less than 1/2CLIA’88 total error,taken as the comparable judging standard,as it satisfied the requirement.For the results out off the reference range,error less than CLIA’88 total error,those still satisfied the requirement.For those items not meet the requirements,it must to do the regression calibration for Vitros350,using Hitachi 7170 as the standard instrument.Results The deviations of 7 items were all less than 1/2CLIA’88 allowed total error,with LDH was 0.16~-9.89,CK 2.92~6.25, ALT -4.64~-8.07,TBIL 0.08~2.67,TP -0.37~4.41,ALB 2.74~4.77 and URIC 1.04~3.0 respectively,and did not need re-calibration.For GLU and CREA,only one out of the reference range sample,the error range was >1/2CLIA’ 88,but

Autonomic Nervous System Diseases ; metabolism ; pathology ; surgery ; Colon ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Enteric Nervous System ; abnormalities ; pathology ; Hirschsprung Disease ; pathology ; Humans ; Infant, Newborn ; Intestinal Diseases ; metabolism ; pathology ; surgery ; Male ; Phosphopyruvate Hydratase ; metabolism ; S100 Proteins ; metabolism

Adult ; Arthroplasty, Replacement, Hip ; Bone Cysts, Aneurysmal ; pathology ; Bone Neoplasms ; metabolism ; pathology ; surgery ; Chondroblastoma ; pathology ; Chondrosarcoma ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Femur Head ; metabolism ; pathology ; surgery ; Follow-Up Studies ; Humans ; Osteosarcoma ; pathology ; S100 Proteins ; metabolism

Adult ; Breast ; pathology ; Breast Neoplasms ; diagnostic imaging ; pathology ; Female ; Humans ; Lymphoma, Non-Hodgkin ; diagnostic imaging ; pathology ; Ultrasonography

Objective:To evaluate the effectiveness for vital pulpotomy in primary teeth with 4 kinds of pulp capping agents.Methods:CHI,CH,GA and FC were chosen as the pulp capping agents for vital pulpotomy in primary teeth and 3 year follow up observation was performed.The teeth in other side served as the controls.The root absorption was observed radiologically.Results:In the group of CHI,CH,GA and FC the clinical effective rate were 100%,87.5%,91.4% and 78.1%,the radiographical success rate were 97%,60%,74.3%and 56.2%,respectively.In the cases treated with CHI showed the highest success rate and significant difference was found among the four groups (P