OBJECTIVE: To introduce and analyze the present status of research on decomposed recipes of traditional Chinese medicine (TCM). METHODS: The relevant literature on the study of the decomposed recipes of TCM in the past five years was collected and analyzed. RESULTS AND CONCLUSION: Studies on the decomposed recipes of TCM have been made from the whole body level to the organ, cell and molecular research level. The pharmacological research in vitro is the main way and quantitative changes of the chemical ingredients have been measured before and after composition. It is very important to combine the modern science and technology in exploring the composing mechanisms of TCM remedies.

Animals ; Disease Models, Animal ; Endothelin-1 ; metabolism ; Male ; Nitric Oxide ; metabolism ; Paraquat ; poisoning ; Rats ; Rats, Sprague-Dawley

Objective To establish an HPLC method for the determination of plasma metformin hydrochloride and an HPLC/ESI-MS method for the determination of plasma glibenclamide in human. Methods The metformin plasma samples were added with acetonitrile to denature proteins and the supernatant was injected into the system directly by using EC 250/4.6 NUCLEOSIL 100-5 analytical column (4.6 mm?250 mm, 5 ?m) with a mobile phase of 0.03 mol/L (NH 4) H 2PO 4∶acetonitrile (75∶25, pH 7.0) at the flow rate of 1.0 ml/min and the detection wavelength of 240 nm. Using glipizide as a internal standard, the glibenclamide plasma samples were extracted with ethyl acetate and determined by LC-MS using a chromatographic column ORBAX SB300C 18 (2.1 mm?150 mm, 5 ?m) with a mobile phase of acetonitrile-0.1% ammonium acetate (55∶45, V/V) at the flow of 0.2 ml/min. Results The glibenclamide concentration had a good linear correlation in the range of 2-200 ng/ml (r=0.999 8) while the metformin concentration in the range of 6.25-4 000 ng/ml (r= 0.999 9 ). Both the inter-day and intra-day RSD of metformin and glibenclamide were lower than 5%. Conclusion The assays have been proved to be sensitive, accurate and convenient and can be used in the study of human pharmacokinetics of glibenclamide and metformin hydrochloride.

Diagnostic Errors ; Humans ; Inflammation ; diagnosis ; etiology ; Laryngeal Neoplasms ; surgery ; Male ; Middle Aged ; Papilloma ; surgery ; Pneumonia ; diagnosis ; Postoperative Complications ; diagnosis

Bacterial Infections ; microbiology ; therapy ; Humans ; Integrative Medicine ; Male ; Microbial Sensitivity Tests ; Middle Aged ; beta-Lactamases ; isolation & purification

Adult ; Breast ; pathology ; Breast Neoplasms ; diagnostic imaging ; pathology ; Female ; Humans ; Lymphoma, Non-Hodgkin ; diagnostic imaging ; pathology ; Ultrasonography

Objective:To evaluate the effectiveness for vital pulpotomy in primary teeth with 4 kinds of pulp capping agents.Methods:CHI,CH,GA and FC were chosen as the pulp capping agents for vital pulpotomy in primary teeth and 3 year follow up observation was performed.The teeth in other side served as the controls.The root absorption was observed radiologically.Results:In the group of CHI,CH,GA and FC the clinical effective rate were 100%,87.5%,91.4% and 78.1%,the radiographical success rate were 97%,60%,74.3%and 56.2%,respectively.In the cases treated with CHI showed the highest success rate and significant difference was found among the four groups (P

Objective Using Hitachi 7170 external quality assessment return target value to evaluate the accuracy of 10 items after Regression calibration of the Vistros 350 dry-type Biochemical Analyzer.Methods The same quality control samples were separately tested on two instruments,and results were reported to the clinical National Center for Clinical Laborato-ries.Substituted the external quality assessment return target value result from the National Center for Clinical Laboratories by using Vitros350 into regression calibration equation,then the getting data were compared with the external quality assess-ment return target value obtained from Hitachi 7170,and the deviation analysis was processed.The total error range from the America Clinical Laboratory Amended Bill was used as the standard.For the results within the reference range,error less than 1/2CLIA’88 total error,taken as the comparable judging standard,as it satisfied the requirement.For the results out off the reference range,error less than CLIA’88 total error,those still satisfied the requirement.For those items not meet the requirements,it must to do the regression calibration for Vitros350,using Hitachi 7170 as the standard instrument.Results The deviations of 7 items were all less than 1/2CLIA’88 allowed total error,with LDH was 0.16~-9.89,CK 2.92~6.25, ALT -4.64~-8.07,TBIL 0.08~2.67,TP -0.37~4.41,ALB 2.74~4.77 and URIC 1.04~3.0 respectively,and did not need re-calibration.For GLU and CREA,only one out of the reference range sample,the error range was >1/2CLIA’ 88,but

Objective To study normal flora components of conjunctiva, explore the relationship between normal flora and ocu- lar bacterial infections.Methods The data of 1 198 bacterial cultures of conjunctiva in non-infectious cataract patients were re- viewed and compared with the results of 985 specimens of conjunctivitis, keratitis and endophthalmitis from January 2000 to December 2004.Results Positive culture of conjunctiva in non-infectious cataract patients was 628 in 1 198 specimens (52.4%).These bacteria including gram-positive cocci and gram-neganve bacilli constituted the normal flora of conjunctiva. Staphylococcus epidermidis was the predominant bacteria (51.1%, 341/667) and its prevalence increased during this 5-year period.S.epidermidis and S.aureus were also the most frequently isolated pathogens in specimens of conjunctivitis, keratitis and endophthalmitis.The correlation was statistically significant between the positive rate in normal eye and conjunctivitis or keratitis in terms of these two species, but not statistically significant between normal eye and endophthalmitis.The resistance rate of normal flora of conjunctiva to ampicillin, amikacin, tobramycin, ciprofloxacin, and ofloxacin increased year by year. The resistance rate to gentamicin and the combination of two drugs decreased.The changing resistant pattern was similar in pa- tients with eye infection.Conclusions There is a close relationship between the normal flora S.aureus and S.epidermidis of conjunctiva and the bacterial infection of ocular surface, but no significant correlation between normal flora and the pathogen of endophthalmitis.

Objective To realize the optimization of image quality and radiation dose by correcting mammography automatic exposure,according to automatic exposure controlled mode of mammography film-screen system . Methods The film-screen system(28 kV) was applied to perform automatic exposure of plexiglass(40 mm) and get the standard dose of exposure, the exposure mode of CR base on LgM =2.0 was rectified, which was divided into 10 steps. Mammary glands pattern(Fluke NA18-220) were examined with CR( 26,28, and 30 kV ) by the automatic exposure mode corrected. The exposure values(mAs) were recorded. CR image was diagnosed and evaluated in double blind way by 4 radiologists according to American Collage of Radiology(ACR) standard. Results Based on the standard of CR automatic exposure with the dose higher than the traditional exposure of film-screen system, the calibration of mammography automatic exposure was accomplished. The test results of the calibrated mode was better than the scoring system of ACR. Conclusions Comparative study showed improvement in acquiring high-quality image and reduction of radiation dose. The corrected mammography automatic exposure mode might be a better method for clinical use.