OBJECTIVE:To compare the bioequiavailability of two simvastatin preparations in human bodies.METHODS:A total of 18 healthy male volunteers were enrolled in a randomized crossover study in which the subjects were randomly assigned to receive single dose of 40mg simvastatin orally disintegrating tablet(test) or simvastatin tablets(reference).The plasma concentrations of simvastatin were determined by LC-MS,and the pharmacokinetic parameters and bioavailability were calculated with 3p97 program.RESULTS:The pharmacokinetics of simvastatin test and reference preparations were fitted the one-compartment model.The main pharmacokinetic parameters of the two preparations were as following:Cmax:(6.73?5.22) vs.(7.08?5.41)ng?mL-1、tmax:(2.11?0.74)vs.(1.89?0.85)h,AUC0～12:(19.83?19.09)vs.(19.98?18.20)ng?h?mL-1,AUC0～∞:(22.18?20.09)vs.(22.41?21.07)ng?h?mL-1.The relative bioavailability of simvastatin orally disintegrating tabl-et as against simvastain tablet(reference) was (99.25?13.11)%.CONCLUSION:Simvastatin test and reference preparations were bioequivalent.

To address the role of Pr1 gene in the process of Hirsutella sinensis infecting Hepialus gonggaensis, differential expression of subtilisin-like protease gene was detected. In the present study, Pr1 gene analogues from H. sinensis were obtained by PCR strategy using specific primers designed from conserved regions of Pr1 gene reported in the GenBank. Then we detected the changes in the expression of Pr1 gene before and after infecting H. gonggaensis using real-time quantitative PCR. We obtained the partial sequence of Pr1 gene with the length of 535 bp (GenBank accession: KC009680). Real-time PCR results showed that the expression level of Pr1 gene was significantly different among 8 samples (P < 0.01). Pr1 gene showed the obvious higher expression level (2-3 folds) after infecting the H. gonggaensis, suggesting that the Pr1 gene may play an important role in the process of H. sinensis infecting H. gonggaensis. The present study paves a way for further identification on infectivity assessment of H. sinensis.

ObjectiveTo improve the diagnosis and treatment of primary duodenal carcinoma.MethodThe clinical data of 45 patients with primary carcinoma of the duodenum from 1987 to 2000 were analyzed retrospectively.ResultAccording to the site of cancer, tumors around, below and above the duodenal papilla accounted for 64%, 22% and 13% of all cases. The diagnosis rate of duodenoscopy was 71%(15/21). Pancreatoduodenectomy was performed in 22 cases, segmental duodenectomy in 2 cases, simple tumor resection in 3 cases, gastroenterostomy or choledochojejunostomy in 12 cases and simple laparotomy in 4 cases. In patients of curative resection, the postoperative 3 and 5 years′ survival rate was 41 4% and 23 6%. In those with gastroenterostomy or choledochojejunostomy, the average survival time was 6～15 months. All cases undergoing biopsy only died within six months.ConclusionPrimary duodenal carcinoma mainly occurs around duodenal papilla. The long time survival of Whipple′s procedure is favourable.

Objective To study the diagnostic value of single detection and combined detection of serum NSE ,CYFRA21‐1 , CA50 ,SCC and CEA in the patients with esophageal squamous cell carcinoma .Methods The single detection and combined detec‐tion of serum NSE ,CYFRA21‐1 ,CA50 ,SCC and CEA levels were measured in 110 cases of esophageal squamous cell carcinoma and 101 health controls in our hospital from October 2013 to October 2015 .The detection results were statistically analyzed . Results The positive rate and expression levels of serum NSE ,CYFRA21‐1 ,CA50 ,SCC and CEA in the esophageal squamous cell carcinoma group were higher than those in the health control group with statistical significance(P<0 .05) .The sensitivity and diag‐nostic efficiency of combined detection of NSE ,CYFRA21‐1 ,CA50 ,SCC and CEA for diagnosing esophageal squamous cell carcino‐ma were 93 .64% and 86 .73% respectively .Conclusion The 5 tumor markers combined detection of serum NSE ,CYFRA21‐1 , CA50 ,SCC and CEA can improve the sensitivity and diagnostic efficiency for diagnosing esophageal squamous cell carcinoma .

Objective: To comparative the mechanical properties of f our kinds of denture resin. Method: The impact strength, f lexural strength, flexural elastic modulus and Brinell hardness of four denture resin materials, Shanghai Shanhu(SH),Shanxi Changzhi(CZ), Luciton 199(LC) and Me liodent(MD), were measured in vitro and the stress-strain curve of each ma terial under flexural force was drawn. Results: The impact strength(kJ/m 2) of SH,CZ,LC and MD were 9.94?2.25,6.55?1.85,13.76?4.31 and 13.70?3.71; flexural strength (MPa) 75.27?3.24,78.88?7.66,80.47?3.27 and 7 6.72?3.14; flexural elastic modulus(GPa) 3.08? 0.15 ,2.96?0.10,2.63?0.11 a nd 2.56?0.12; Brinell hardness(kg/mm 2)24.15?1.34, 23.43 ? 1.65 ,20.86? 1.26 and 18.49?1.71,respectively. Conclusions: Comparativ ely speaking, SH and CZ are rigidit and brittle, LC is rigidit and sturdy, MD is tough and sturdy.

BACKGROUND: Glucose is an important factor on differentiation of pancreatic duct stem cells, it relates to the quantity and secretion function of insulin-producing cells after differentiation.OBJECTIVE: To compare the insulin secretion capacity of the differentiated rat pancreatic stem cells induced by various glucose concentrations.METHODS: Rat stem cells were isolated and purified from pancreatic duct cells using collagenase V and Ficoll-400. These stem cells were randomly divided into 10 groups. Every group was induced to culture, proliferate, differentiate and form insulin- producing cells in vitro. The differentiation of all groups was performed in medium with different concentrations of glucose. The immunofluorescence staining was used to identify the pancreatic duct stem cells. The electrochemical luminescence method was used to detect the insulin release from stem cell differentiated islets.RESULTS AND CONCLUSION: The stimulation index of glucose 20.6, 25.6, 30.6 mmol/L groups was higher than that in other groups (P < 0.05), but there was no difference between each two groups among these three groups (P > 0.05). The insulin releasing of glucose 15.6, 20.6, 25.6 groups was higher than that in other groups (P < 0.05), but there was no difference between each two groups among these three groups (P > 0.05). The best insulin secretion capacity of insulin-producing cells can be gained by controlling concentration of glucose as 20.6-25.6 mmol/L when pancreatic duct stem cells differentiated into insulin-producing cells.

Objective To analyze the effect of clinical pathway management for capillary bronchitis in Department of Pediatrics.Methods 75 children with capillary bronchitis after the implementation of clinical pathway management were selected as the observation group,and 68 children with capillary bronchitis before the implementa-tion of clinical pathway management were selected as the control group.The hospitalization cost,average hospital stay, antibiotic use rate,cure rate,nonsocomial infection rate,rehospitalization rate within two weeks,service satisfaction and other comprehensive indices were compared.Result The total cost of drug of the observation group[(1 198.49 ± 397.48)yuan]was lower than (1 324.05 ±376.57)yuan of the control group(Z =2.00,P <0.05).The average hospital stay of the observation group[(6.99 ±2.39)d]was shorter than (8.01 ±2.22)d of the control group(Z =2.62,P <0.05).The service satisfaction rate of the observation group(95%)was higher than 82% of the control group(χ2 =5.44,P <0.05).The antimicrobial use rate of the observation group(49%)was apparently lower than 95% of the control group (χ2 =27.59,P <0.01).There were no statistically significant differences between the two groups in cure rate(hospital discharge rate),nosocomial infection rate and rehospitalization rate within two weeks(all P >0.05).Conclusion Clinical pathway management for capillary bronchitis in Department of Pediatrics of primary hospital can play a role in standardizing medical practice,reducing average hospitalization days,controlling medical cost,and improving service satisfaction under the precondition of ensuring medical quality.

Dried roots and rhizomes of Salvia miltiorrhiza (Danshen) are among the most commonly used traditional Chinese medicines in clinic. The material basis for its efficacy mainly includes hydrophobic tanshinones and hydrophilic salvianolic acids. The traditional effects of Danshen are "removing stasis and relieving pain, activating blood to promote menstruation, clearing heart fire and tranquilization". According to modern pharmacological studies, Danshen and its main components have cardiovascular and cerebrovascular protective effect. Recent studies showed that Danshen and its main components also demonstrated protective effects on liver injury models induced by carbon tetrachloride, D-galactosamine, acetaminophen and alcohol. In this paper, the hepatoprotective effect and mechanism of Danshen were summarized and studied.
Animals ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Liver ; drug effects ; Protective Agents ; chemistry ; pharmacology ; Salvia miltiorrhiza ; chemistry

Adult ; Chlorine ; poisoning ; Humans ; Male ; Middle Aged ; Phosgene ; poisoning ; Respiratory Distress Syndrome, Adult ; chemically induced

Abnormalities, Multiple ; blood ; diagnosis ; metabolism ; physiopathology ; Child ; Diagnosis, Differential ; Dwarfism ; blood ; diagnosis ; metabolism ; physiopathology ; Estradiol ; blood ; Female ; Follicle Stimulating Hormone ; blood ; Humans ; Luteinizing Hormone ; blood ; Silver-Russell Syndrome ; blood ; diagnosis ; metabolism ; physiopathology