Objective:To investigate the effects of Yizhi Oral Solution on protein and RNA synthesis of brain tissue of mice. Methods: The radioactive intensities of 3H leucine in brain protein and 3H uridine in RNA of brain tissue of young mice were observed by the methods of incorporation in vivo and group control observation.Results: The radioaetive intensity of 3H leucine of Yizhi Oral Solution group was higher than that of cycloheximide pathological model group ( P

In order to observe the febrifugal response of needling "Chang Qiang Point", and analyse its mechanism lumbar spinal cord of rabbits were damaged in the expermental study. The experimental findings were as follows: 1. It was found that cAMP content in csf was striking increased, when leukocytic pyrogen fever of rabbits was significant. During needling "Chang Qiang Point" of febrile rabbits, however, febrile response was markedly inhibited(at 60 min~?T℃ was 0.23?0.07 and TRI4 0.61?0.03). There was significant difference(P0.05). 3. After damaging lumbar spinal cord of rabbits, there were certain change of rectal temperature and the febrile response, but it had no effect on cAMP content in csf. The authors infered that the impulses from "Chang Qiang Point" transmiled Via spinal cord might be closely associated with reflex mechanism and the change of cAMP content might play an important role in the mechanism of febrifugal effect during needling "Chang Qiang Point" in febrile rabbits.

Objective To explore the role of ripply1 in zebrafish dorsal-ventral development .Methods Using ze-brafish whole-mount in situ hybridization to examine the ripply1 expression pattern in early embryo development .To analyse the expression pattern changes of dorsal-ventral marker genes at shield stage and the morphological changes at 24 hpf (hours post-fertilization) after overexpression of ripply1 by injecting synthetic mRNA at 1-cell stage.Using Tol2 transposon technology to obtain a ripply1 promoter driven GFP transgenic fish and to identify promoter region that recapitulates endoge -nous ripply1 expression pattern .Results The in situ hybridization results revealed that ripply1 specifically expresses in the future dorsal region at shield stage .Overexpression of ripply1 caused an enhanced expression of dorsal marker genes and a reduction of ventral marker genes .Embryos overexpressing ripply1 also showed severely dorsalized phenotype , with enlarged head, reduced ventral yolk extension , and shortened posterior trunk and tail regions , and the formation of a secondary trunk axis.Transgenic fish revealed the maternal expression of ripply1 and suggested that a 1.2 kb promoter-driven GFP is able to recapitulate the endogenous gene expression pattern .Conclusion ripply1 may participate in the early development of dor-sal-ventral axis in zebrafish embryo .

Objective To evaluate the therapeutic effects of combining acupuncture and physical therapy with drugs on treating Duchenne muscular dystrophy (DMD).Methods Sixty infants with DMD were randomly divided into a treatment group and a control group (n =30 in each).Both groups were injected with Bozhi glycopeptide and given levocamitine and fructose sodium diphosphate orally.Moreover,the treatment group was additionally given acupuncture and physical therapy.Before and after 90 days of treatment,the time to walk 10 metres and to climb stairs of 2 metres high was measured,as were the serum creatine phosphate kinase (CPK),lactate dehydrogenase (LDH) and aspertate aminotransferase (AST) levels of all participants.Results After treatment,the average walking and stair climbing times of both groups were significantly shorter,and those of the treatment group were significantly shorter than those of the control group.Moreover,the average CPK,LDH and AST levels in the treatment group were significantly lower than before treatment and than those of the control group after treatment.There was a significant difference in the overall response rates,with 93％ observed in the treatment group and 73％ in the control group.Conclusion Combining acupuncture and physical therapy with drugs can significantly increase the mobility and improve the serum CPK,LDH and AST levels of children with DMD.

Objective: For preparing the reference material of alkaline phosphatase(ALP), the purification and properties of ALP from porcine kidney were studied. Methods: The ALP purification procedure included isolation microvillus of porcine kidney cortices by solubilization the membrance-binding enzymes with 1-butanol, precipitation with ammonium sulfate, chromatography on DEAE-Sephacel,ConA-Sepharose,Sephadex G-200 and a immuno-affinity column of anti-GGT antibodies. Results: The purified enzyme had a specific activity of 402 kU/g protein at 37℃and was almost free of contaminating enzymes. The apparent Michaelis constants was 1.35 mmol/L, and the optimum pH was 10.40. Conclusion: The kinetic properties of the preparation were very close to those of the enzyme present in the human serum, The product was suitable as enzyme preparation for producing the enzyme reference material.

AIM:To evaluate the effect of Huazhuo Jiedu Huoxue Recipe(Herba Agastaches,Herba Eupatorii,Rhizoma Pinelliae,Pericarpium Citri reticulatae,Poria,etc.) on plasma orphanin FQ(OFQ) and prostaglandins in the patients of endometriosis(EM) with pain.METHODS:The concentrations of plasma OFQ and prostaglandins were measured by radioimmunoassay before and after the treatment on the patients of EM with pain.29 patients of EM with severe pain(severe group),31 patients with slight pain(slight group) and 25 patients without pain(control group) were studied.RESULTS:The levels of OFQ、 PGF_(2a)、 PGE_2、 6-keto-PGF_(1a) and TXB_2 in plasma were significantly higher in patients of EM with pain than that of patients without pain(P

Objective To investigate the influence of Zengshiling oral liquid on gastric emptying,plasma motilin (MOT) and small intestine propulsion of young rat. Methods Experiment animal were divided into five groups randomly:control group,large dose of Zengshiling oral liquid group,normal dose of Zengshiling oral liquid group,small dose of Zengshiling oral liquid group and Motilium group. The rats were gavaged by 0.9% of NaCl and corresponding drug. 7 days later,gastric emptying was assayed by phenol red,plasma MOT levels and carbon powder propelling rate of small intestine were also assayed. Results The rate of gastric emptying of Zengshiling oral liquid groups were significantly higher than control group (P

Objective To study the expression and cellular localization of STAT3 and SOCS-3 in the motor neurons of normal rat spinal cord. Methods Immunocytochemistry and morphometry methods were used in the present study. Results STAT3 immunocytochemical staining was mainly distributed in the cytoplasm of the motor neurons of the ventral horns, SOCS-3 immunoreactive products were extensively distributed in the neurons of the ventral and dorsal horns, glia and fibers of the spinal cord. In the ventral horn, SOCS-3 immunoreactivity was found in the nuclei and/or cytoplasm of the motor neurons.Conclusions STAT3 and SOCS-3 are extensively distributed in the normal adult rat spinal cord and SOCS-3 is existence in the forms of nuclear or cytoplasmic protein.

Objective Finding characteristic of senile colon diverticula in 70 cases senile colon diverticula diagnosed by colonoscope through comparing and summarizing. Methods From Jan 1997 to Dec 2001, 70 cases older than 60 years old senile colon diverticula diagnosed by colonoscope in endoscopy department of PLA general hospital. Male 54 cases ,female 16 cases .Age from 60 to 84 years old, average age 68.70?5.39. Results ①With age increased, detectable rate of colon diverticula and multiple colon diverticula rised; ②Detectable rate in male higher than female; ③Whether the single or multiple diverticula ,all predilection site is in the right colon, rate of the right to the left is 2.8∶1(42∶15), and the single diverticula is more obvious. Conclusions ①Occurrence of diverticula have relation to age; ②Incidence in the right colon of senile is lower than of young people; ③The left colon diverticula and bilateral colon diverticula in senile is higher than in young people.

Objective: To study stress distribution and displacement by different distract directioos on mandibular corpus with finite element method. Methods:A three-dimensional finite element model of mandibular distraction osteogenesis was established.Von Mises stress as well as the displac ement of gnathion and gonion under different loads were measured. Result s:The stress and displacement were positively related with distract forc e. Von Mises stress primarily accumulated in distracted areas and were higher un der bilateral distraction parallel to the occlusial plane. Gnathion and gonion w ere positively displaced on X and Z axes and negatively displaced on Y axis. Distraction parallel to the mandibular body induced positive displaceme nt of mandible on X and Y axes and negative on Z axis. Concl usion:Transversal displacement to the opposite side is greater during un ilateral distraction, while displacement on the saggittal plane is greater durin g bilateral distraction.