Objective To investigate the effect of autologous transplantation of circulating endothelial progenitor cells (EPCs) on oleic acid-induced acute lung injury (ALI) in rabbits. Methods Thirty New Zealand long ear rabbits weighing 1.8-2.0 kg were randomly divided into 2 groups ( n = 15 each): normal saline group (group NS) and EPC group. ALI was induced by iv oleic acid 80 mg/kg. EPC (106/200 μl) or equal volume of normal saline (NS) was administered iv at 4 h after iv oleic acid injection. Arterial blood samples were obtained before (T0) and at 4, 8, 12, 24 and 48 h (T1-5) after oleic acid injection for blood gas analysis and determination of serum vascular endothelial growth factor (VEGF) concentration. The animals were then scrificed (at T5 ) and the lungs were removed for microscopic examination and determination of W/D lung weight ratio, Pulmonary infiltration of PMN and non-PMN was counted and hyaline membrane formation and hemorrhage were examined. Results PaO2/FaO2 ratio and serum VEGF concentration were significantly higher in group EPC than in group NS. Infiltration of leukocytes in the lung was significantly reduced by EPC transplantation. EPC also decreased lung water content, hyaline membrane formation and hemorrhage in the lungs. Conclusion Autologous transplantation of circulating EPC can ameliorate oleic acid-induced acute lung injury in rabbits.

BACKGROUND: For the purpose of keeping the merits of traditional actual cautery, we improved traditional actual cautery and developed a pistollike electrocautery which has the function of heating and spraying oilpreparation (it has been issued national patent, patent number: 17634; patent number of medical electrocautery: 872106187). OBJECTIVE: To observe the effect of unique electrocautery of traditional Chinese medicine (TCM) on treating chronic tonsillitis as compared with cautery.DESIGN: Random sampling and case-controlled study.SETTING: Department of Otolaryngology, Affiliated Hospital of Liaoning College of Traditional Chinese Medicine; Surgical Department of Liaoning Oncology Hospital.PARTICIPANTS: Totally 80 patients with hypertrophy of tonsil were selected from the Department of Otolaryngology of the Affiliated Hospital of Liaoning College of Traditional Chinese Medicine in 1989. Inclusion criteria: degrees Ⅱ and Ⅲ of tonsil hypertrophy, without other serious cardiovascular and cerebral diseases, and consent. Exclusion criteria: attempted cauterization, cease of treatment, incomplete case history having complication, and not acceptance. Totally 40 patients were coincidence with the criteria including 22 males and 18 females aged from 5 to 40 years.METHODS: Mild and potent cauterization was adopted according to whether there was chronic congestion in tonsil. For patients with degree Ⅲ of tonsil hypertrophy without obvious congestion, potent cauterization with large size cautery was adopted at 700-800 ℃, if with chronic congestion,potent cauterization with large or middle size cautery at 500-600 ℃ and mild cauterization was adopted; For patients with degree Ⅱ of hypertrophy of tonsil without obvious congestion, potent cauterization with middle size cautery was adopted at 700-800 ℃, if with chronic congestion, mild cauterization with middle or small size cautery was adopted at 500-600 ℃; for patient with first degree of hypertrophy of tonsil without obvious congestion, potent cauterization with small size cautery was adopted at 600-700 ℃, if with chronic congestion, mild cauterization with small size cautery was used at 500 ℃. Cauterization of TCM was not of rather good therapeutic effect on degree Ⅰ of tonsil hypertrophy than on degrees Ⅱ and Ⅲ of hypertrophy of tonsil. Regardless of which degree of hypertrophy of tonsil and congestion, mild cauterization should be adopted at low degree of heat if there was pain radiating to the ear region or pharyngopalatine arch becoming surface-like structure. The average interval between each cauterization was 2-3 days.MAIN OUTCOME MEASURES: Size of tonsil and histological changes of patients with degrees of Ⅱ and Ⅲll tonsil hypertrophy after cauterization.RESULTS: Totally 40 patients with degrees Ⅱ and Ⅲ of tonsil hypertrophy were treated with cautery without any loss. ① Tissues were thickened after each cautery, 38 cases received only 4-10 times of cauterization, and half of the total number of the patients received 7-8 times of cauterization (9/22.5%, 9/22.5%). ② Electrocautery could reduce course of treatment to 1/4 of that of actual cautery. Degree of hypertrophy of tonsil was of no direct ratio to cauterizing times in that 22 patients with degree Ⅱ of hypertrophy of tonsil received 4-10 times of cauterization; while 18 cases of patients with degree Ⅲ of hypertrophy, 2 patients received 13 times of cauterization, the other received 7-10 times of cauterization. ③ A patch of black and brown cauterized scab was formed after each electrocautery on the surface of tonsil; superfical layer was of faveolate structure with different sized eosin dyed mesh; the deep layer was of dull grey oblique and interdigitating cord like structure with necrotic tissues without being dyed and survival lymphocyte in the space between cord, the lymphocyte had the changes of karyo pyknosis and nuclear fragmentation or disappearing of red dyed nuclear. Compared with actual cautery, cauterization of necrotic layer was obviously thickening and easy to exfoliate in electrocautery of TCM. ④ After 24 hours of cauterization, necrotic tissues migrated to normal lymphatic area presented with such inflammatory changes as vascular dilation, widening of lymphocyte space and infiltration of neutrophil. The degrees of above mentioned changes between electrocautery of TCM and actual cautery achieve no significant difference and the inflammatory changes disappeared in 4-5 days.CONCLUSION: Because of high temperature, few cauterized times and short course, electrocautery of TCM has a remarkable effect on degrees Ⅱ and Ⅲ of tonsil hypertrophy; however, various textures, degrees of hyperemia, ages and co-operations can cause different cauterized times.

History, 20th Century ; History, 21st Century ; Periodontics ; history

Arginine ; therapeutic use ; Burns ; drug therapy ; immunology ; Critical Care ; methods ; Critical Illness ; therapy ; Glutamine ; therapeutic use ; Humans ; Linolenic Acids ; therapeutic use ; Nutritional Support ; methods ; Wounds and Injuries ; drug therapy ; immunology

Objective To explore the isometric attachment site of the posterolateral structure in the femoral lateral condyle and introduce the method of knee posterolateral complex(PLC) reconstruction with the semitendinosus tendon. Methods Isometric reconstruction of the chronic PLC injury of knee joint in 18 cases, were performed with the semitendinosus tendon from February 2001 to February 2005. Of all patients, there were 12 males and 6 females, average age was 39 years old(range, 19-52 years old). All cases were chronic injuries, with duration of 1.5-14 months. 3 cases were simple PLC injury, 10 cases were PLC injury with posterior cruciate ligament(PCL) injury, 2 of which had undergone PCL reconstruction about 6.5 months. 3 cases combined with anterior cruciate ligament(ACL) injury, 2 cases combined with ACL and PCL injury. To put in the double semitendinosus tendon from the bony tunnel of anatomical insertion of the fibular collateral ligment in caput fibulae and put out from the bony tunnel of anatomical insertion of the popliterofibular ligment in the caput fibulae, then fix them respectively in the isometic bony tunnel of the femoral lateral condyle. The posterolateral stability of the knee was evaluated mainly through examination varus instability of knee and external rotation range of leg. Results It was relatively isometric from the femoral attachment site of the popliteal tendon to the anatomical insertion of the popliterofibular ligment in the caput fibulae. The better isometric attachment site was from the anatomical caput fibulae attachment site to 8-10 mm in front of the anatomical femoral attachment site of the lateral collateral ligament. The average follow-up period was 14 months(12-24 months). There was no varus knee instability in full extension. At 30? flexion, one-grade varus instability was found in 2. At 30? and 90? flexion, external rotation range increased by 6? in 3 cases, remained the same in 14 cases, and decreased in 1 case, all compared with the healthy side. Conclusion The method provided good stability for the knee and acquired satisfactory clinical results.

Objective To evaluate the value of MR-guided neurolytic celiac plexus block(NCPB)for treatment of upper abdominal cancer pain.Methods 13 neurolytic celiac plexus blocks were carried out in 12 patients with severe upper abdominal pain caused by malignant tumors.The pain-relieving effect of the block was both evaluated using visual analogue scale(VAS) and analyzed statistically in all cases.Results The placement of the needle MR-guided was easy and accurate,the successful rate of the puncture was 92%.There were no severe complications.The pain before and after the procedure had obvious difference and the pain relief could last for a long time.Conclusion MR-guided NCPB is a simple and effective technique for treatment of upper abdominal cancer pain.

Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems.The gene that encoding.HCV E2 protein was cloned in pQE30 and pEF1/HisC.After the expression of E2 protein in E.Coli M15 and COS 7 cell,the expressed proteins were used to detect their antigenicity with ELISA and WB.The results showed that protein E2 was expressed in both prokaryotic and eucaryotic cells.Special reaction could be detected using the expressed proteins and sera from HCV infected people.The studied E2 gene could express the desired proteins in both prokaryotic and eucaryotic expression systems,and glycosylation of the E2 protein happened in COS 7 cell.

AIM To study the effects of neferine on gastric car cinoma apoptosis induced by vincristine in vitro. METHODS Cytotoxicity assay was tested by MTT method. The influence of neferine to affect vincristine to induce gastric carcinoma apoptosis was detected by PI staining flow cytometry, AO/EB double fluorescence stain and terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL). RESULTS 2 5, 5,10 ?mol?L -1 neferine enhanced vincristine to inhibit the proliferation of SGC7901 cells; 10 ?mol?L -1 neferine enhanced vincristine (0 1, 0 5, 2, 4 mg?L -1 ) to induce SGC7901 cells apoptosis. CONCLUSIONS Neferine enhanced vincristine to induce gastric carcinoma cells apoptosis. It is inferred a kind of low poisonous and high effective chemosenstizers.

Objective To establish a multi-parameter diagosis criteria for Myelodysplastic syndromes(MDS).Methods Eight diagnostic parameters for MDS obtained from our retrospective study were prospectively studied.From Jan.2000 to June 2004,leukemic transformations in 38 MDS patients diagnosed according to the multi-parameter criteria plus WHO criteria were dynamically followed up,and compared with that in 79 MDS cases who were diagnosed only according to WHO criteria contemporaneously and that in 123 MDS cases historially who fit in with WHO criteria.Results Eighteen of the 38 MDS patients transferred into leukemia.Among the 79 MDS patients diagnosed only according to the WHO criteria,18 transferred(P

Objective To optimize an extracting technology for Fufang Baige capsule. Methods The orthogonal design was used for the inspection of water-extracting process and alcohol-precipitating process. Water volume, extracting time and extracting times were screened as inspection elements for water-extracting process. And liquid concentration, alcohol-precipitating concentration and incubating time were screened as inspection elements for alcohol-precipitating process. The transferring rate of paeoniflorin which is the indicator component in drug-Jun was screened as the inspection indicator for each fraction of extraction process. Results The optimal extraction conditions were as follows:for water- extracting process, that was extracting 2 times with 15-fold water, 2 hours for each time ; For alcohol-precipitating process, that was concentrating liquid to 1.0 g/mL Chinese herbs containing, then adding alcohol untill alcohol concentration ammouted to 70%, and at last putting it static for 18 hours. Conclusion The extracting technology was simple, economical and practical, and was able to increase production efficiency.