Objective To investigate the effect of autologous transplantation of circulating endothelial progenitor cells (EPCs) on oleic acid-induced acute lung injury (ALI) in rabbits. Methods Thirty New Zealand long ear rabbits weighing 1.8-2.0 kg were randomly divided into 2 groups ( n = 15 each): normal saline group (group NS) and EPC group. ALI was induced by iv oleic acid 80 mg/kg. EPC (106/200 μl) or equal volume of normal saline (NS) was administered iv at 4 h after iv oleic acid injection. Arterial blood samples were obtained before (T0) and at 4, 8, 12, 24 and 48 h (T1-5) after oleic acid injection for blood gas analysis and determination of serum vascular endothelial growth factor (VEGF) concentration. The animals were then scrificed (at T5 ) and the lungs were removed for microscopic examination and determination of W/D lung weight ratio, Pulmonary infiltration of PMN and non-PMN was counted and hyaline membrane formation and hemorrhage were examined. Results PaO2/FaO2 ratio and serum VEGF concentration were significantly higher in group EPC than in group NS. Infiltration of leukocytes in the lung was significantly reduced by EPC transplantation. EPC also decreased lung water content, hyaline membrane formation and hemorrhage in the lungs. Conclusion Autologous transplantation of circulating EPC can ameliorate oleic acid-induced acute lung injury in rabbits.

BACKGROUND: For the purpose of keeping the merits of traditional actual cautery, we improved traditional actual cautery and developed a pistollike electrocautery which has the function of heating and spraying oilpreparation (it has been issued national patent, patent number: 17634; patent number of medical electrocautery: 872106187). OBJECTIVE: To observe the effect of unique electrocautery of traditional Chinese medicine (TCM) on treating chronic tonsillitis as compared with cautery.DESIGN: Random sampling and case-controlled study.SETTING: Department of Otolaryngology, Affiliated Hospital of Liaoning College of Traditional Chinese Medicine; Surgical Department of Liaoning Oncology Hospital.PARTICIPANTS: Totally 80 patients with hypertrophy of tonsil were selected from the Department of Otolaryngology of the Affiliated Hospital of Liaoning College of Traditional Chinese Medicine in 1989. Inclusion criteria: degrees Ⅱ and Ⅲ of tonsil hypertrophy, without other serious cardiovascular and cerebral diseases, and consent. Exclusion criteria: attempted cauterization, cease of treatment, incomplete case history having complication, and not acceptance. Totally 40 patients were coincidence with the criteria including 22 males and 18 females aged from 5 to 40 years.METHODS: Mild and potent cauterization was adopted according to whether there was chronic congestion in tonsil. For patients with degree Ⅲ of tonsil hypertrophy without obvious congestion, potent cauterization with large size cautery was adopted at 700-800 ℃, if with chronic congestion,potent cauterization with large or middle size cautery at 500-600 ℃ and mild cauterization was adopted; For patients with degree Ⅱ of hypertrophy of tonsil without obvious congestion, potent cauterization with middle size cautery was adopted at 700-800 ℃, if with chronic congestion, mild cauterization with middle or small size cautery was adopted at 500-600 ℃; for patient with first degree of hypertrophy of tonsil without obvious congestion, potent cauterization with small size cautery was adopted at 600-700 ℃, if with chronic congestion, mild cauterization with small size cautery was used at 500 ℃. Cauterization of TCM was not of rather good therapeutic effect on degree Ⅰ of tonsil hypertrophy than on degrees Ⅱ and Ⅲ of hypertrophy of tonsil. Regardless of which degree of hypertrophy of tonsil and congestion, mild cauterization should be adopted at low degree of heat if there was pain radiating to the ear region or pharyngopalatine arch becoming surface-like structure. The average interval between each cauterization was 2-3 days.MAIN OUTCOME MEASURES: Size of tonsil and histological changes of patients with degrees of Ⅱ and Ⅲll tonsil hypertrophy after cauterization.RESULTS: Totally 40 patients with degrees Ⅱ and Ⅲ of tonsil hypertrophy were treated with cautery without any loss. ① Tissues were thickened after each cautery, 38 cases received only 4-10 times of cauterization, and half of the total number of the patients received 7-8 times of cauterization (9/22.5%, 9/22.5%). ② Electrocautery could reduce course of treatment to 1/4 of that of actual cautery. Degree of hypertrophy of tonsil was of no direct ratio to cauterizing times in that 22 patients with degree Ⅱ of hypertrophy of tonsil received 4-10 times of cauterization; while 18 cases of patients with degree Ⅲ of hypertrophy, 2 patients received 13 times of cauterization, the other received 7-10 times of cauterization. ③ A patch of black and brown cauterized scab was formed after each electrocautery on the surface of tonsil; superfical layer was of faveolate structure with different sized eosin dyed mesh; the deep layer was of dull grey oblique and interdigitating cord like structure with necrotic tissues without being dyed and survival lymphocyte in the space between cord, the lymphocyte had the changes of karyo pyknosis and nuclear fragmentation or disappearing of red dyed nuclear. Compared with actual cautery, cauterization of necrotic layer was obviously thickening and easy to exfoliate in electrocautery of TCM. ④ After 24 hours of cauterization, necrotic tissues migrated to normal lymphatic area presented with such inflammatory changes as vascular dilation, widening of lymphocyte space and infiltration of neutrophil. The degrees of above mentioned changes between electrocautery of TCM and actual cautery achieve no significant difference and the inflammatory changes disappeared in 4-5 days.CONCLUSION: Because of high temperature, few cauterized times and short course, electrocautery of TCM has a remarkable effect on degrees Ⅱ and Ⅲ of tonsil hypertrophy; however, various textures, degrees of hyperemia, ages and co-operations can cause different cauterized times.

History, 20th Century ; History, 21st Century ; Periodontics ; history

The accreditation of Proficiency Testing Provider ( PTP) has gone through more than a decade in China.Over the past ten years, from nonexistence to existence, the domestic PTPs in the area of laboratory medicine have gradually standardized and developed.By reviewing the international and domestic practice, this paper gives an outline of the present status of the accreditation of PTP in the area of laboratory medicine, explores the difference with other countries, the main problems and some suggestions for improvement, and makes a prospect of the development of the accreditation of PTP in the area of laboratory medicine in China.

Objective To explore the correlation between adverse childhood experiences, personality characteris?tics and alcohol dependence. Methods A total of 102 patients with alcohol-dependence (Patients group) and 106 volun?teers (control group) were rated by the adverse childhood experience scale (ACEs) and the big five personality scale (BFP). Results The prevalence of ACEs are significant higher in patients group than in control group, especially regard?ing emotional abuse, physical neglect, witness violence and substance abuse. The patients group has lower scores of de?light, conscientiousness and higher scores of psychoticism. Psychoticism and parents abuse alcohol in childhood were the risk factors of alcohol dependence. Conclusions There is a correlation between alcohol dependence with their ACEs, per?sonality characteristics and parents abused substance. The psychoticism increase the risk of alcohol dependence.

Objective: To evaluate the therapeutic effect of combining use of acupuncture and drugs on rheumatoid arthritis(RA) in the early and the active stages of by the imaging of bone and joint with 99m TC-methlene diphosphate(MDP). Methods: 102 cases of RA in the early and the active stages were randomly allocated into the compound Fengshining group (Group A),acupuncture group(Group B), and compound Fengshining plus acupuncture group(Group C).They were treated for 30 days. All patients were examined before treatment and after treatment by 99m TC-MDP imaging of the whole body, hands and feet, and analyzing method of random interested region was used to count the ratio of radioactivity in the affected area of RA to radioactivity in adjacent normal bone(T/TN). Meanwhile, joint swelling index, joint tenderness index, morning stiffness, gripping force, and erythrocyte sedimentation rate (ESR) were also observed. Results: T/TN of the Group A after treatments had no significant difference comparing with that before treatment(t = 1.35, P＞0.05), while the Group B and C had significant differences(t = 5.31, t=8.97, P＜0.01). The changes of clinical and experimental examination in the Group C had more significant differences than those in the Group A and B(P＜0.05).Conclusion: Acupuncture therapy could promote RA recovery, and combining use of acupuncture and compound Fengshining had better effect.

Objective To describe the echocardiographic and clinical findings of patients with accessory mitral valve(AMV).Methods Four adult patients were diagnosed to have AMV by Doppler echocardiography.Results All the four patients had cardiac murmur but 2 were asymptomatic.Echocardiogram showed that two cases had isolated AMV with significant left ventricular outflow tract (LVOT)obstruction.Another patient was complicated by idiopathic hypertrophic subaortic stenosis and his systolic pressure gradient across the LVOT reduced to normal after taking negative inotropic drugs.The fourth patient was associated with complex congenital cardiac anomalies but without LVOT obstruction.Conclusions AMV may not be very rare as previously reported.An echocardiographic examination Can detect AMV and difierentiate it from other causes of LVOT obstruction.

Objectives To study the survival,growth and function restoration of fetal ovary allo transplant. Methods Murine fetal ovaries were grafted subcutaneously and under renal capsule to spayed adult female rats.On day 5,10,25-35,beyond 35 after transplantation,the grafts were removed and underwent histological and histochemical examination. Serum estrodiol and progesterone levels were determined. Results It can be divided three periods according the HE staining:On the day 5 of transplantation, there were many blood cells in the graft.On day 10,there were a lot of primordial and primary follicles in ovarian cortex of the graft.On day 25 and later,there were follicles in different developing stages, corpora luteas and interstitial glands in the graft.The positive reaction of 3-β-HSDH and oil red O and the results of serum estrodiol and progesterone showed that transplanted fetal ovary restored secreting function. Conclusions Fetal ovary allotransplant can be survival,develop and restore the function of secreting sexhormone.

Objective To explore the isometric attachment site of the posterolateral structure in the femoral lateral condyle and introduce the method of knee posterolateral complex(PLC) reconstruction with the semitendinosus tendon. Methods Isometric reconstruction of the chronic PLC injury of knee joint in 18 cases, were performed with the semitendinosus tendon from February 2001 to February 2005. Of all patients, there were 12 males and 6 females, average age was 39 years old(range, 19-52 years old). All cases were chronic injuries, with duration of 1.5-14 months. 3 cases were simple PLC injury, 10 cases were PLC injury with posterior cruciate ligament(PCL) injury, 2 of which had undergone PCL reconstruction about 6.5 months. 3 cases combined with anterior cruciate ligament(ACL) injury, 2 cases combined with ACL and PCL injury. To put in the double semitendinosus tendon from the bony tunnel of anatomical insertion of the fibular collateral ligment in caput fibulae and put out from the bony tunnel of anatomical insertion of the popliterofibular ligment in the caput fibulae, then fix them respectively in the isometic bony tunnel of the femoral lateral condyle. The posterolateral stability of the knee was evaluated mainly through examination varus instability of knee and external rotation range of leg. Results It was relatively isometric from the femoral attachment site of the popliteal tendon to the anatomical insertion of the popliterofibular ligment in the caput fibulae. The better isometric attachment site was from the anatomical caput fibulae attachment site to 8-10 mm in front of the anatomical femoral attachment site of the lateral collateral ligament. The average follow-up period was 14 months(12-24 months). There was no varus knee instability in full extension. At 30? flexion, one-grade varus instability was found in 2. At 30? and 90? flexion, external rotation range increased by 6? in 3 cases, remained the same in 14 cases, and decreased in 1 case, all compared with the healthy side. Conclusion The method provided good stability for the knee and acquired satisfactory clinical results.

Objective To evaluate the value of MR-guided neurolytic celiac plexus block(NCPB)for treatment of upper abdominal cancer pain.Methods 13 neurolytic celiac plexus blocks were carried out in 12 patients with severe upper abdominal pain caused by malignant tumors.The pain-relieving effect of the block was both evaluated using visual analogue scale(VAS) and analyzed statistically in all cases.Results The placement of the needle MR-guided was easy and accurate,the successful rate of the puncture was 92%.There were no severe complications.The pain before and after the procedure had obvious difference and the pain relief could last for a long time.Conclusion MR-guided NCPB is a simple and effective technique for treatment of upper abdominal cancer pain.