Objective To investigate the effects of long-term consumption of Fallopia multiflora on mouse hematopoietic system.Methods Forty 10-month old female C57BL/6J mice were equally divided into two groups at random, the control group fed with normal food , and the experimental group , given food with added Fallopia multiflora. After 10 month, the mice were sacrificed, and the peripheral blood, spleen, thymus and bone marrow cells were examined by flow cytometry.Results In the mice fed with Fallopia multiflora, the percentage of B cells in the spleen and CD 4 +cells in the thymus were increased , and CD8 + cells in the thymus and bone marrow hematopoietic stem cells were decreased , among the bone marrow cells , G0 cells were increased , but G1 and G2/S/M cells decreased .Conclusions Long-term proper consumption of Fallopia multiflora can delay the ageing of the hematopoietic system , and sustain its stability.

Objective: To observe the effect of ultrasound ablation combined with the balloon angioplasty in treatment of patients with arteriosclerosis obliteration of lower extremity and diabetic foot. Methods: The 60 limbs of 47 patients with arteriosclerosis obliteration of lower extremity (AOLE) were treated comprehensively by anti-infection,enlarging blood vessel, improving circulation,and anticoagulation; they also received ultrasound ablation,balloon angioplasty and stenting. Results: The patent rate of the 60 limbs was 93.3%. One month and 3 months after treatment with ultrasound ablation combined with balloon angioplasty,the MRI of artery of lower extremity,ultrasound manifestation,ankle-brachial index (ABI),the skin temperature of lower extremity and the sense to coolness,numbness,pain,ochrodermia or cyanosed were all improved to some extents compared with those before treatment. The healing of the diabetic foot was also accelerated. Conclusion: Ultrasound ablation combined with the balloon angioplasty and stenting, together with conventional treatment like anti-infection, anticoagulation, enlarging blood vessel, improving circulation, can reopen the occluded blood vessels and improve blood supply, thus provides a favourable condition for treatment of diabetic foot.

BACKGROUND:With the development of tissue engineering, porous bioceramics are more and more used to repair bone defects. Current research focuses on the biological synthesis of this bioceramics and its performance evaluation.
OBJECTIVE:To discuss the preparation of a new kind of bone cement and to determine its physicochemical properties and biocompatibility with osteoblasts.
METHODS:Biphasic tricalcium phosphate powders were prepared using co-precipitation method. The powder was turned into granular stuff by arabic gum. After sintering, porous hydroxyapatite/tricalcium phosphate bioceramics were harvested, and then mixed with alpha-hemihydrate to prepare the bone cement.
RESULTS AND CONCLUSION:X-ray diffraction confirmed that the synthetic substance was a kind of biphasic calcium phosphate ceramic having a porous structure. The bone cement could be in the plastic state within 3 minutes. The curing time was 15 minutes, and the curing temperature was 36.5℃. The maximum compression strength was 5.82 MPa, and the MTT toxicity was level 0. Osteoblasts could grew wel on the material surface.

ObjectiveTo study the etiology, preoperative diagnosis and treatment of nonocclusive mesenteric ischemia(NOMI). MethodsClinical data of 3 cases of NOMI from 1992 to 1998 were analyzed retrospectively. ResultsMajor clinical presentations were abdominal pain, gastrointestinal bleeding and abdominal irritation. None was correctly diagnosed preoperatively. Partial ileectomy in 2 cases and right colectomy in 1 case was performed with a mortality of 2/3. Conclusion Early diagnosis and accurate assessment of the bowel viability in exploration were quite difficult. Resection of the infarcted bowel or ′second-look procedure′ were recommended if necessary.

Objective To reinforce management in training various kinds of medical personnel and improve training efficiency.Methods Data related to factors influencing the transfer efficiency of medical personnel training were gathered by means of interviews with and questionnaire surveys of participants in hospital training in Tianjin and processed with the statistical software of SPSS 10.0.Then factors significantly relevant to the transfer efficiency of medical personnel training were identified.Results Measures for increasing the transfer efficiency of medical personnel training were put forward from such aspects as designing training programs, creating sound organizational environments,defining training objectives,choosing training methods,selecting trainers,and strengthening medical personnel's self-motivation.Conclusion The above-mentioned measures can effectively increase the transfer efficiency of medical personnel training.

Objective To make clear the relationship between the genetic polymorphism of MMP-1 and implant early failure.Methods We conducted a case control study,53 cases patients (no smoking history and without periodontitis) who have underwent dental implant were divided into experimental group(with dental implant failure) and control group(with dental implant success) according to early failure of dental implant (within one month).DNA of 53 patients in Han nationality were extracted by using Chelex-100 method and polymorphisms of the mmp-1-1607 gene were detected by restriction fragment length polymorphism after polymerase chain reactions.Results Patients with MMP-1-1607(1G/2G) genotype exhibited a significantly higher occurrence of implant early failure than those with MMP-1-1607(1G/1G) gene type(P＜0.05).The OR of the 1G/2G versus the 1G/1G of the MMP-1-1607 genotype was 13.894(P＜0.05).Conclusion MMP-1-1607(1G/2G) genotype is associated with implant early failure.

Objective To assess the effect of immunosuppression conversion from cyclosporine(CsA) to tacrolimus(FK506) on progression of chronic allograft nephropathy(CAN).Methods A retrospective study was performed in 36 cyclosporine treated renal transplant recipients.Patients were included if they were biopsy-proven CAN and they were converted from cyclosporine to tacrolimus due to CAN.The FK506 dose was 1/50 to 1/100 of the cyclosporine's and the dose was adjusted according to the trough level of tacrolimus,body weight of the patient and the situation of CAN.Other immunosuppressive agents kept unchanging.Serum creatinine and FK506 trough level were monitored after conversion.Fasting blood triglyceride,total cholesterol and glucose were determined at baseline and 6th month after conversion to tacrolimus.Results After switching over to FK506 for 6 months,there was a significant improvement in function of renal allograf(P

To investigate the change in renal CD11a and myeloperoxidase (MPO) contents and renal function during ischemia-reperfusion injury (IRI) of rat kidney, as well as the role of lymphocyte function associated antigen-1 (LFA-1) played in renal IRI, we utilized a rat model of renal IRI to detect the renal tissue contents of CDlla and MPO and renal functioa The results showed that the levels of CD11a and MPO were very low in normal renal tissue, but increased significantly in ischemia reperfusion. Renal failure was presented in rats with IRI. The levels in the ischemia-60min-reperfusion group were higher than those in the ischemia-30min-reperfusion group in the same time points. Our conclusion is the levels of CD11a and MPO in rat renal tissue increase significantly during renal IRI. LFA-1-mediated leukocyte adherence plays an important role in renal IRI. Up-regulation of CDlla further elucidates the mechanism of relation between IRI and acute rejection: more heavier IRI is, more possible acute rejection would be.

Objective To clarify the actions of tranilast,an anti allergic drug,on chronic constriction mononeuropathy through an experiment using an animal model according to the method of Bennett et al. Methods 36 rats were divided into 2 groups: one was treated with tranilast(200mg/kg?d-1,p.o every experimental day),the other was controlled with solvent only. Chronological changes of heat evoked withdrawal latencies on a hot plate,nerve coducting velocitics, and histopathological changes were compared between the two groups from one to four weeks after loose ligation in the sciatic nerve. Results The changes of the withdrawal latency and nerve conducting velocities in the tranilast treatment group were significantly longer than those in the control. The rats without tranilast treatment showed inflammation in and around the constricted nerve bundles,axonal degeneration,phagocytes invasion and interstitial edematous changes at 7 days,numerous axonal sprouts and remyelination at 14 days,and regeneration in the nerve bundles at 28 days. In contrast,the rats treated with tranilast showed less inflammation or nerve fiber degeneration,and better regeneration than those of the control. Conclusion The actions of tranilast appear to have beneficial effects on prophylaxis of a chronic constriction mononeuropathy and on preservation of the nerve functions in rats.

Objective To compare the usefulness of contrast-enhanced T1 high resolution isotropic volume excitation (THRIVE) sequence and fat-saturated TSE sequence in detection of spinal metastases.Methods Thirty-one consecutive patients with spinal metastases were recruited.All patients received post-contrast TSE T1WI followed by a fat-suppressed THRIVE sequence.The number of lesions,SNR and CNR for both sequences,and the scoring of image quality concerning motion-artifact and tumor conspicuity were compared.Results Acquisition time of the post-contrast TSE T1W sequence and the THRIVE sequence was 2 min 55 s and 33 s,respectively.No significant difference was found in the number of lesions detected between the two sequences (Z=-0.816,P=0.414).The SNR (432.54±271.60) and CNR (233.27± 197.65) of the THRIVE sequence were significantly lower than the SNR (674.32±375.79) and CNR (312.38±207.49) of the TSE T1W sequence respectively (t=-4.366,-2.660,P＜0.001,0.012).Tumor conspicuity of the post-contrast TSE sequence was better than that of THRIVE sequence (Z=-4.082,P＜0.001),while the motion-artifact in TSE sequence was more severe (Z=2.291,P=0.022).Conclusion The post-contrast THRIVE sequence is capable of decreasing acquisition time and motion-artifact.Besides,its detected efficacy is equal to that of TSE sequence.There is practical possibility to replace the conventional post-contrast TSE T1WI sequence with the THRIVE sequence in the imaging of spinal metastases.