Nutritional and metabolic disorders are the common problems in PICU. They often lead to deterioration in child nutrition and vice versa. Reasonable assessment of the energy consumption can provide theoretical basis for proper nutrition support in critically ill children. In this review, the methods of evaluating the energy consumption which included energy metabolism measurement and prediction equations were introduced, and the importance of indirect calorimetry was emphasized. Meanwhile, the research situation of energy consumption assessment at home and abroad and the current status of its clinical application were also reviewed.

AIM:To study the effects of Se-methylselenocysteine(MSC) on the growth and apoptosis of human liver carcinoma cell line,HepG_2 cells and the molecular mechanism of effects.METHODS: After HepG_2 cells treated with various concentrations of MSC,survival and apoptosis were determined by MTT assay and light microscope,apoptosis and cell cycle were determined by flow cytometry.Malignant phenotype was determined by soft agarose growth assay.Cyclin D1 mRNA expression was determined by RT-PCR and Caspase-3 activity was measured by Caspase-3 activity assay kit.RESULTS: After being treated with 25(?mol?L~(-1)) MSC for 24 h,the survival of HepG_2 cells was decreased,a marked apoptosis and S phase arrest characteristic was observed in time-and dose-dependent manner.Soft agatose growth assay showed MSC inhibited HepG_2 cells growth in soft agarose.RT-PCR and Caspase-3 assay showed that HepG_2 cells treated with 25(?mol?L~(-1)) MSC for 24 and 48 hours,the expression of Cyclin D1 mRNA was down-regulated by 38% and 47%,while Caspase-3 activity was up-regulated by((39.61)?(6.65))%and((118.73)?(6.48))%.HepG_2 cells treated with 50(?mol?L~(-1)) MSC for 24 and 48 h,the expression of Cyclin D1 mRNA was down-regulated by 53% and 82%, whereas Caspase-3 activity was up-regulated by((80.66)?(9.31))% and((152.67)?(7.95))%.CONCLUSION: MSC can inhibit the growth of HepG_2 cells,and induce apoptosis and S phase arrest of HepG_2 cells.The phenotype alterations of HepG_2 cells might relate with inhibition of Cyclin D1 expression and Caspase-3 activity by MSC in the cells.

Objective: To study the effect of combining bcr abl Aspo and c myb Aspo on K562 cells. Methods: Cells were exposed to oligomers. Cell inhibitory rate was determined by typan blue dye exclusion. CFU K562 cells were cultured in 0.8% methylcellulose. P210 was measured by flow cytometry. Cellular bcr abl mRNA was detected by RT PCR semiquantitative analysis. Cell apoptosis was measured by flow cytometry and observed by electron microscope. Results: When the concentration of both bcr abl Aspo and c myb Aspo was 5 ?mol/L, K562 cells were still growth in clone state. The growth inhibitory rate was 61 7% at 120 h. P210 was depressed at 24 h and went up to 25.7% at 120 h. The apoptosis rate was 22.5%. While K562 cells were dealt with 10 ?mol/L bcr abl Aspo and 10 ?mol/L c myb Aspo, the cells were growth in dispersal. The cell growth inhibitory rate reached to 92.2% and 64.3% of K562 cells were induced to apoptosis at 120 h. P210 was complelely depressed untill 120 h. The decrease of bcr abl mRNA was from 69.2% to 85.3% after incubation 48 h with 5 ?mol/L Aspo and 10 ?mol/L. Conclusion: It emerges coordination to combine bcr abl Aspo and c myb Aspo on K562 cells, and enhances the anti leukemia effect.

53 male Wistar rats were divided into three groups, i.e. experimental ulcer group, saline control group and normal control group. The antral tissues were prepared for immunohistochemical staining at 4th, 10th, 14th, 21st and 28th days after operation. Sternberger's PAP method was used to demonstrate the gastrin cells (G cells) and somatostatin cells (D cells)in the antral mucosa in order to observe their changes during experimental gastric ulcer. The morphological relationships of G cells and D cells were examined by simultaneous double immunostaining method.The results indicated that the G cells count as well as D cells count in the antral mucosa was increased (P

Cadherins that mediate the adhesion of the same type of cells by specific binding to calciumdependent adhesions of the same type with cadherins mainly consist of three subtypes including E-cadberin,P-cadherin and N-cadherin.In recent years,more and more studies have indicated that cadherins are closely related to triple-negative breast cancer(TNBC)and play an important role in prognosis and treatment of TNBC.

Objective: To study the effect of combining bcr-abl Aspo and c-myb Aspo on K562 cells. Methods: Cellswere exposed to oligomers. Cell inhibitory rate was determined by typan blue dye exclusion. CFU-K562 cells were culturedin 0.8% methyleellulose. P210 was measured by flow cytometry. Cellular bcr-abl mRNA was detected by RT-PCR semiquantitative analysis. Cell apoptosis was measured by flow cytometry and observed by electron microscope. Results: When the concentration of both bcr-abl Aspo and c-myb Aspo was 5 μmol/L, K562 cells were still growth in clone state. The growth inhibitory rate was 61.7% at 120 h. P210 was depressed at 24 h and went up to 25.7% at 120 h. The apoptosis rate was 22.5%. While K562 cells were dealt with 10 μmol/L bcr-abl Aspo and 10 μmol/L c-myb Aspo, the cells were growth in dispersal. The cell growth inhibitory rate reached to 92.2% and 64.3% of K562 cells were induced to apoptosisat 120 h. P210 was complelely depressed untill 120 h. The decrease of bcr-abl mRNA was from 69.2% to 85.3% after incubation 48 h with 5 μmol/L Aspo and 10 mol/L. Conclusion: It emerges coordination to combine bcr-abl Aspo and c-myb Aspo on K562 cells, and enhances the anti-leukemia effect.

Objective To analysis the features of Montreal classification,serum markers,treatment and prognosis of Chinese patients with Crohn's disease(CD),and to estimate the relationships between clinical classification and anti-sarccharomyces cerevisiae antibody(ASCA),treatment and prognosis.Methods A retrospective study of 102 consecutive definite CD eases were performed and all subjects were classified into subtypes according to Montreal classification.The results of ASCA,extra-intestinal manifestation,treatment and disease behavior at follow-up were recorded and compared among different subtypes.Results The A2 subtype(52.9%)was dominated in Chinese CD patients.Ileocolon location(40.2%)and stricture lesion (53.9%)were common.The complication rate was dependent on disease course(P＜0.05),and intestinal fistula was associated with disease location(P=0.074).B1 subtype had higher progressive rate than B2 subtype(P=0.018).ASCA was not associated with disease loeation,disease behavior,treatment and disease progression(P＞0.05).Conclusions Crohn's disease mainly attack young people with male predominance.Early-onset CD patients have higher ASCA positive rate and disease progressive rate.The disease behavior progresses associated with disease course,and the rate of complication and the increase of surgery.The penetrating behavior is the main cause for surgery.Montreal classification is useful to predict the disease course,the need for surgery as well as the prognosis.

Animals ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Humans ; Idarubicin ; administration & dosage ; Leupeptins ; administration & dosage ; Neoplasms ; drug therapy ; pathology ; Neoplastic Stem Cells ; drug effects ; metabolism ; pathology ; physiology

Pregnancy complicated by Marfan syndrome (MFS) could increase adverse pregnancy outcomes. The most serious MFS-induced complication is aortic dissection (AoD), which could endanger both the mother and the fetus. This article summarizes the research progress in China and overseas in the diagnosis and treatment of pregnancy complicated by MFS in recent years in order to raise awareness and improve prenatal diagnosis and pre-conception counseling, thereby facilitating early diagnosis and timely perinatal multi-disciplinary standardized treatment with a view to improving the prognosis.

In recent years, with the changes of human living environment, the morbidity of respiratory tiact allergic diseases such as allergic rhinitis, infant wheezing, allergic asthma, and other diseases increased rapidly, thereby affecting the quality of children′s life, and increasing heavy burdens to families and society.Microbial infection, including virus, bacteria, mycoplasma pneumoniae/chlamydia, fungi and parasites, can regulate the occurrence and development of respiratory tract allergic diseases through the immune mechanism.In order to deepen the understanding of the relationship between respiratory allergy and microbial infection and its related mechanism, the research progress on the relationship between respiratory allergy and microbial infection was reviewed in this paper.