1.Reliability and validity of Chinese Pediatric Quality of Life Inventory in influenza cases
WANG Shenyu ; GAN Zhengkai ; HU Xiaosong ; SHAO Yanzhi ; CHEN Yingping ; LIANG Zhenzhen ; XING Bo ; CHEN Zhiping ; LÜ ; Huakun
Journal of Preventive Medicine 2020;32(5):471-474
Objective:
To explore the reliability and validity of the Chinese Pediatric Quality of Life Inventory(PedsQL)in influenza children.
Methods:
From January 2017 to February 2018,we selected laboratory-diagnosed influenza cases and healthy children according to age and gender ratio by stratified random sampling and systematic sampling method. We employed Chinese version of PedsQL 4.0 to investigate their quality of life,used Cronbach's α to evaluate the reliability,and used Pearson correlation coefficient, t-test,confirmatory factor analysis(CFA)and ROC curve to evaluate the validity.
Results:
Totally 300 influenza cases and 300 healthy children were surveyed,with 294(98.00%)and 295(98.33%)valid questionnaires recovered. The general Cronbach's α was 0.89,and the Cronbach's α of each dimension ranged from 0.79 to 0.84. The Pearson correlation coefficients between the items and their belonged dimension ranged from 0.537 to 0.755,between the items and other dimension ranged from 0.203 to 0.384. The CFA resulted in RMSEA of 0.06,GFI of 0.88,AGFI of 0.90,CFI of 0.91 and NFI of 0.89. The scores in total and in all the dimensions in influenza cases were significantly different with those in healthy children(P<0.05). Taking the scores in influenza cases as a golden standard,the area under the ROC curve was 0. 985(P<0.05),the sensitivity was 0.92,and the specificity was 0.95. When the limit score was 82.18,the Youden index was the largest.
Conclusion
The Chinese version of PedsQL4.0 has good reliability and validity, which can be applied to quality of life assessment in children.
2. Disease burden of influenza in schools and child care settings in rural areas of Hangzhou, 2016-2018
Shenyu WANG ; Zhengkai GAN ; Yanzhi SHAO ; Zhiping CHEN ; Huakun LYU
Chinese Journal of Preventive Medicine 2019;53(7):713-718
Objective:
The objective of this study was to analyze the disease burden of influenza in schools and child care settings in rural areas of Hangzhou.
Methods:
Hospital visit influenza cases aged 3-17 years in hospitals that reported based on influenza surveillance system from 2016 to 2018 in Chun′an county, Hangzhou city were selected as study subjects and a total of 294 confirmed cases of influenza were selected using system sampling method. Questionnaires were designed to investigate the basic information and data on inpatients and outpatients among, health care and life quality, etc.. Direct and indirect economic burden and disability adjusted life year (DALY) were analyzed and compared among different age groups.
Results:
The mean age of investigated subjects was (8.88±3.92) years. A total of 143 (48.64%) investigated cases were male. In total of 283 (96.26%) cases were outpatients. The total economic burden was 124 743.95 CNY. The mean economic burden was 424.30 CNY per person. The mean direct and indirect economic burden was 361.33 and 62.97 CNY per person respectively. The difference of the mean direct, indirect and total economic burden per person between different age group was statistically significant (
3.Intracellular codelivery of anti-inflammatory drug and anti-miR 155 to treat inflammatory disease.
Chao TENG ; Chenshi LIN ; Feifei HUANG ; Xuyang XING ; Shenyu CHEN ; Ling YE ; Helena S AZEVEDO ; Chenjie XU ; Zhengfeng WU ; Zhongjian CHEN ; Wei HE
Acta Pharmaceutica Sinica B 2020;10(8):1521-1533
Atherosclerosis (AS) is a lipid-driven chronic inflammatory disease occurring at the arterial subendothelial space. Macrophages play a critical role in the initiation and development of AS. Herein, targeted codelivery of anti-miR 155 and anti-inflammatory baicalein is exploited to polarize macrophages toward M2 phenotype, inhibit inflammation and treat AS. The codelivery system consists of a carrier-free strategy (drug-delivering-drug, DDD), fabricated by loading anti-miR155 on baicalein nanocrystals, named as baicalein nanorods (BNRs), followed by sialic acid coating to target macrophages. The codelivery system, with a diameter of 150 nm, enables efficient intracellular delivery of anti-miR155 and polarizes M1 to M2, while markedly lowers the level of inflammatory factors and . In particular, intracellular fate assay reveals that the codelivery system allows for sustained drug release over time after internalization. Moreover, due to prolonged blood circulation and improved accumulation at the AS plaque, the codelivery system significantly alleviates AS in animal model by increasing the artery lumen diameter, reducing blood pressure, promoting M2 polarization, inhibiting secretion of inflammatory factors and decreasing blood lipids. Taken together, the codelivery could potentially be used to treat vascular inflammation.