Objective To clone and express hypervariable region 1 (HVR1) fragments of different genotypes of HCV strains from different cities of China.Then detect the reactivity of the expressed HVR1 fusion proteins with sera of chronic he-patitis C patients to analyzing it's signification in clinical utilization. Methods HVR1 genes of four HCV strains (genotype 1b and 2a) were amplified from pGEMT-E2 plasmids and cloned into pQE40 vectors, respectively to construct four recombinant prokaryotic expression plasmids which expressed HVR1 as fusion proteins with DHFR in Escherichia coli strain TG1. Then we used the purified DHFR-HVR1 proteins to detect the anti-HVR1 antibodies in 70 serum samples of chronic hepatitis C patients. Results Four DHFR- HVR1 fusion proteins were successfully expressed in E.coli (320～ 800 ?g fusion proteins per 100 ml culture). Each fusion protein (SH1b, BJ1b, SD1b and SD2a) reacted with 72.8%(51/70), 60%(42/70), 48.6%(34/70), and 58.6%(41/30) of the anti-HCV positive patients' sera, respectively by ELISA. 57% (4/7) of the non responders' sera taken before therapy reacted with all four HVR1 fusion proteins, while only 15.3% (2/13) of the sera from responders reacted with all of them. The A values of sera from IFN therapy responders were significantly higher than non responders (P

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.

Objective To explore quality evaluation system for standardized training of general residents based on the six core competencies of World Organization of Family Doctors(WONCA).Methods The literatures meeting the requirements were selected by literature screening method under the framework of WONCA's six core competencies.The qualitative data were collected in the form of open questionnaires,and the indicator system was determined after two rounds of consultation.The evaluation indexes for residents training quality were selected by Colaizzi 7-step analysis method based on phenomenology.The weights of all indexes were calculated by analytic hierarchy process(AHP)to formulate quality evaluation system.Results 33 indexes in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.)were determined to construct quality evaluation system for standardized training of general residents.The weights of all indexes were determined by AHP.In all the indexes,weights of trainees'income(0.082),superiors attention(0.081)and the total completion assessment(0.113)were the highest.Conclusion The research results show that in the evaluation of quality system for standardized training of general residents,it is necessary to pay close attentions to the indexes with the highest weights in the 10 dimensions(information about residents,understanding about training content,basic information about the training hospital,et al.),especially for trainees'income,superiors attention and the total completion assessment to improve the evaluation system.