Objective To explore the effect of Kangfuxin Fluid by local infiltration for preventing the postoperative infection and hemorrhage after after cold knife conization (CKC) in the patients with grade Ⅲ cervical intraepithelial neoplasia (CIN).Methods Eighty cases of grade Ⅲ CIN treated by CKC in this hospital from January 2015 to December 2016 were selected and randomly divided into the control group and observation group,40 cases in each group.The observation group conduct the Kangfuxin Fluid infiltrated gauze for compression hemostasis after CKC,while the control group used the povidone iodine infiltrated gauze for conducting compression hemostasis.The short term complications (fever infection rate,vaginal bleeding time and cervical wound healing time) and long term complications(cervical adhesion/narrow,late bleeding) were observed and compared between the two groups.Results The incidence of postoperative fever infection,vaginal bleeding time,wound healing time and rate of cervical adhesion in the observation group were lower than those in the control group,and the differences were statistically significant (P＜0.05);the late bleeding occurrence rate had no statistical difference between the two groups(P＞0.05).Conclusion Kangfuxin Fluid can effectively prevent the complications such as wound infection after CKC,and has clinical application value.