Objective: To explore the infection of mycoplasma in the venereal diseases and information about their drug susceptibility. 　Methods: Materials from the 12 528 patients were cultivated and drug susceptibility made testing was made using the mycoplasma IST Kit.　Results: The results showed that 9433 of 12 528 (75.3%)were infected by mycoplasma, Uu infective rate 58.3 %(7 305 cases)was found significantly higher than Mh infective rate 3.0%(379 cases)and mixed infection of both Uu and Mh was 14.0%(1 749 cases,)P＜0.01. The no susceptible conditions were in turn that ofloxacine(50.5%), Erythromycine(41.9%), tetracycline(14.6%), doxycycline(4.7%), josamycine(2.9%), and pristinamycine(2.0%) respectively.　Conclusions:The results suggested that non-gonococcal genitourinary tract infection was firstly ureaplasma urealyticum, concentracted infection was at age 21-40 years. no susceptible stat of mycoplasma infection to drug was serious, so that susceptibility testing of mycoplasma had a important significance to clinical drug treatment.

The therapeutic effect and mechanism of moxibustion in the treatment of Herpes Zoster were studied. It was found that the level of T lymphocyte subsets of the patients with Herpes Zoster was within the normal range before treatment, but slightly lower than in the health group. Th cells and NK cells were significantly decreased in the patients with Herpes Zoster as compared with those in the health group (P＜0. 05),suggesting the cellular immunity of the patients with Herpes Zoster was decreased. After treatment with moxibustion, the level of all the T lymphocyte subsets was increased, close to the normal. Among them the levels of Th cells and NK cells were significantly different from those before the treatment (P＜0. 05). The disappearance of exanthema and relief of neuralgia in the patients was also quicker than in the controls (P＜0. 01). It was indicated that moxibustion had a good effect in the treatment of Herpes Zoster, which was contributed to the enhancement of the body antiviral ability by the improvement of immunity, especially the cellular immunity.

Objective To investigate signal transduction mechanism of phospholipase C?1 (PLC?1) in colorectal cancer cells. Methods Gel electrophoresis mobility shift assay (EMSA), immunocytochemistry, zymography and RT-PCR were performed to investigate the function of PLC?1 on nuclear factor-Kappa B (NF-?B), matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in LoVo cell. Results Compared with control group, nuclear positive rate of cells treated with epidermal growth factor (EGF) increased significantly (from 26.91%?2.84% to 40.83%?4.36%), while that of cells treated with 2.5mol/L U73122 decreased to 12.20%?1.89%. Meanwhile, pretreatment with 2.5mol/L U73122 before EGF treatment decreased nuclear positive rate of cells from 40.83%?4.36% to 18.21%?1.34%. The results of EMSA further verified that PLC?1 can regulate the activity of NF-?B. RT-PCR results showed that EGF, PLC?1 or NF-?B had no significant effect on the expression of MMP-2, TIMP-2 at mRNA level. Furthermore, zymography indicated that the activity of MMP-2 did not change dramatically by EGF, PLC?1 or NF-?B. Conclusion These data suggested that EGF-PLC?1-NF-?B signaling pathway was operative in LoVo cell, but MMP-2 and TIMP-2 may not be regulated by EGFR-PLC?1-NF-?B signaling pathway.

Objective:To observe the clinical effect and safety of diammonium glycyrrhetate(DG) in the treatment of psoriasis vulgaris.　Methods:48 patients were randornly divided into two groups treated group(28 cases)and controlled group(20 cases). Both were given DG or common drugs, respectively.　Results:Effective rate was 67.9%(19/28), mean effect-begining time was 17.42±2.36 days in the treated group, they were significantly increased in comparison with controlled group(Effective rate was 30.0%, mean effect-begining time was 25.65±3.54 days). Any side effect was not occurred in treated group. 　Conclusion:DG is effective and safe in the treatment patients with psoriasis vulgaris.

Objective To investigate the expression of fragile histid ine triad (FHIT)gene and its relationship with the proliferation and apoptosis of tumor cells in cutaneous malignant melanoma (CMM).Methods The expression o f FHIT gene and PCNA were detected by streptavidin peroxidase method with skin s pecimens taken from 57 primary cutaneous melanoma and 20 normal controls.Apopto sis of tumor cells was detected by terminal deoxynuclneotidyl transferase mediat ed dUTP nick end labeling (TUNEL).Results The expression level of FHIT protei n was significantly lower in CMM than that in the normal skin tissue (P

Objective: To investigate the function and mechanism of phospholipase Cγ1 (PLCγ1) in cell-matrix adhesion in colorectal cancer. Methods: Highly metastatic colorectal cancer cell line LoVo and lowly metastatic colorectal cancer cell line SW480 were subjected to cell-matrix adhesion assay. U73122 (a specific inhibitor of PLC) and pyrrolidine dithiocarbamate (PDTC) (an inhibitor of NF-κB) were used to study the effect of PLCγ1 and NF-κB on cell-matrix adhesion. Furthermore, Western blot and gel electrophoresis mobility shift assay (EMSA) were performed to detect the mechanism of PLCγ1 in colorectal cancer cell adhesion to matrix. Results: Inhibition of PLCγ1 or NF-κB resulted in reduction of cell-matrix adhesion in a dose-dependent manner in LoVo cells(P<0.05), but had no marked effect on SW480 cells. Western blot analysis showed that epidermal growth factor (EGF) stimulated the phosphorylation of PLCγ1 in LoVo. The results of EMSA indicated that inhibition of PLCγ1 signaling pathway also down-regulated the activity of NF-κB while EGF reversed the function. Conclusion:These data suggest that PLCγ1 plays a pivotal role in the EGF-induced cell-matrix adhesion of highly metastatic colorectal cancer cells and that NF-κB is also functional in this signaling pathway.

The cell culture technology is the basis for the postgraduate students to undertake researches in furure.The department of cell biology of our university carried out the teaching reformation on the cell culture course by means of writing outstanding teaching materials, making supporting DVD, arranging suitable teaching contents and plans and setting up flexible examining systems, which promoted the teaching effects and profited students to master this skill quickly.

Objective: To preare recombinant lentivirus stably suppressing PLC?1 in human colorectal carcinomas Lo-Vo cells, so as to establish LoVo cell lines deficient in PLC1 and to investigate the role of this gene. Methods: Recombinant lentivirus produceing PLC-?1 siRNA were prepared. After LoVo cells were transduced with lentivirust, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC?1 was examined by Western-blot and RT-PCR analysis. The effect of the lentivirus on the cell proliferation and cell adhesion was analyzed by XTT method and ctll adhesion assay, respectively. Results: PLC?1 siRNA knocked down PLC?1 expression in LoVo cells obviously. The silenced efficiency of siRNA transducted by recombinant lentivirus was very high. Adhesion of human colorectal carcinomas LoVo cell Lines was significantly decreased, while proliferation was not affected. Conclusion: Our research confirm that PLC?1 plays an important role in cell adhesion of colorectal carcinomas, and provides experimental evidences for targeting PLC?1 in gene therapy against cancer.

Objective: To investigate the function and mechanism of phospholipase C?1 (PLC?1) in cell-matrix adhesion in colorectal cancer. Methods: Highly metastatic colorectal cancer cell line LoVo and lowly metastatic colorectal cancer cell line SW480 were subjected to cell-matrix adhesion assay. U73122 (a specific inhibitor of PLC) and pyrrolidine dithiocarbamate (PDTC) (an inhibitor of NF-?B) were used to study the effect of PLC?1 and NF-?B on cell-matrix adhesion. Furthermore, Western blot and gel electrophoresis mobility shift assay (EMSA) were performed to detect the mechanism of PLC?1 in colorectal cancer cell adhesion to matrix. Results: Inhibition of PLC?1 or NF-?B resulted in reduction of cell-matrix adhesion in a dose-dependent manner in LoVo cells(P

Objective To investigate the effects of different combinations of vitamin (Vit) C, Vit E and GSH on the biochemical markers and bone mineral density (BMD) in ovariectomized rats. Methods Seventy female SD rats aged 4 months were divided into two groups, 20 rats with sham operation in control group and 50 rats with bilateral oophorectomy in model group. 3 months later, 10 rats in the model group and 10 rats in the control group were randomly selected and their body weight, uterus weight, BMD of the left femurs and lumbar spines,biomeehanical characteristics of the left femurs, serum levels of Ca2+ , creatinine (Cr), alkaline phosphatase (ALP),superoxide dismutase (SOD) , malondialdehyde (MDA) , GSH-peroxidase (GSH-Px) and serum resisting abilities to OH- were determined. Then the rest rats were divided into five groups: A (sham), B (OVX control), C (Vit C +Vit E), D (GSH) and E (Vit C +Vit E +GSH). Vit C, Vit E and GSH were given 750rng/kg, 250 mg/kg, and 125 mg/kg, respectively daily for 3 months. And then the biochemical markers and BMD were measured. Results 3 months after treatment with antioxidants, BMD of left femurs and lumbars spines was increased, while the level of serum ALP was decreased markedly in B, C and D group as compared with that in B group. The serum level of SOD, GSH-Px and serum resisting ability to OH- were increased in D and E groups and the level of MDA decreased in C and D groups as compared with that in B group. Conclusion Vit C, Vit E and GSH increased BMD, prevented the decrease of SOD and GSH-Px and elevated serum resisting ability to OH-in ovariectomized rats.