Objective To investigate the clinical effectiveness of hysteroscopy in cervical conization for benign lesions. Methods A total of 1026 patients with benign cervical lesions were treated by cervical conization under a hysteroscope from January 2000 to December 2006. Results The operation time was 5-25 min and the intraoperative blood loss was 5-100 ml. The patients received re-examination 3 menstrual cycles after the operation. Among them, 990 patients were cured (cure rate,96.5%); the other 36 patients (34 improved and 2 ineffective) were cured after the second operation. No surgery related complications occurred. Conclusion Hysteroscopy is effective for cervical conization. The method is worth being widely used for fewer complications.

Objective To construct the bait plasmid of HNP-3 mature peptide in yeast two-hybrid system and examine whether the recombinant bait plasmid has self-activating and toxicity effect.Methods Using RT-PCR technique,the cDNA fragments of HNP-3 mature peptide gene were amplified from the extracted RNA in cultured HL-60 cells.The fragment was firstly cloned into pBluescript-SK-II vector,confirmed by sequencing,then sub-cloned into the bait plasmid pGBKT7 and identified with PCR and sequence analysis techniques.The recombinant plasmid was introduced into the yeast cell AH109,and its self-activating and toxicity effect was tested by auxotrophic selective culture.Results DNA sequencing indicated that the inserted fragment in pBluescript-SK-II vector was HNP-3 mature peptide gene sequence,and the sub-cloned recombinant pGBKT7-HNP-3 was no mismatch.The recombinant bait plasmid didn't have self-activating effect and did not show toxicity to yeast AH109 cell.Conclusion The bait plasmid of HNP-3 mature peptide was constructed successfully.This was helpful for investigating the proteins interacting with HNP-3 mature peptide by yeast two-hybrid technique.