Objective:To explore the status and characteristic of psychology for primipara and gravida with second child so as to improve psychological status of them and take them keep positive mentality in whole gestation.Methods: 176 gravidas randomly were selected in this study, and a self-designed questionnaire of psychology was applied to evaluate and compare the psychological status and self-care capacity between primipara and gravida with second child by using method of questionnaire survey. The primipara and gravida with second child were implemented hierarchical management depended on their differently psychological problem, and personalized intervening measurement was adopted to enhance their self-care capacity.Results: The psychological enduring capacity, self-care capacity and the ability of deal with problem about interpersonal relationship of gravidas with second child were obviously better than that of primiparas, while the neglect degree for antenatal care of them was higher than that of primiparas.Conclusion: The whole society should pay attention to the process for gestation of pregnant woman, and give them higher social support so as to promote the maternal and child health and effectively decrease the occurrence rate of delivery risk.

Objective To observe the level of urinary microalbumin(MAU) and carotid artery intima-media thickness(CIMT) in patients with subclinical hypothyroidism,and to explore the relationship between subclinical hypothyroidism and MAU,arteriosclerosis.Methods 70 patients with subclinical hypothyroidism were randomly divided into the two groups according to the level of CIMT.33 patients were selected as the arteriosclerosis group and 37 patients were selected as the non-arteriosclerosis group,and 30 healthy people were selected as the control group.The levels of FT3,FT4,TSH,blood fat,MAU,CIMT observed.Results Compared with the healthy controls,the levels of TC,TG,LDL-C,CIMT were significantly higher in the arteriosclerosis group (P =0.008,0.007,0.009,0.006),the level of HDL-C was significantly lower in the arteriosclerosis group(P =0.006).The levels of TC,TG,LDL-C,CIMT were significantly higher in the non-arteriosclerosis group (P =0.042,0.037,0.008,0.035),the level of HDL-C was significantly lower in the non-arteriosclerosis group(P =0.038).The levels of TC,TG,LDL-C,CIMT in arteriosclerosis group were significantly higher than those in the non-arteriosclerosis group (P =0.032,0.041,0.039,0.007).The level of HDL-C in the arteriosclerosis group was significantly lower than the non-arteriosclerosis group(P =0.044).The level of MAU had no significant difference among the three groups(P =0.052,0.087,0.092).TSH had no relationship with MAU (r =0.195,P ＞ 0.05),TSH had positive correlation with CIMT(r =0.578,P ＜ 0.01).Conclusion The patients with subclinical hypothyroidism have lipid metabolism disorder and CIMT incrassation,which can increase the risk of arteriosclerosis.

Objective To discuss the correlation between the distribution of TCM constitutional types and carotid artery intima-media thickness (CIMT) in patients with subclinical hypothyroidism;To select the biased constitution type with high risk factors for atherosclerosis;To provide theoretical basis for preventing this disease from the perspective of TCM physique. Methods Totally 120 patients with subclinical hypothyroidism and 50 cases of normal healthy subjects were selected. TCM constitutional types of two groups were distinguished by KY3H TCM physique recognition software. CIMT was determined, and the correlation between the distribution of TCM constitutional types and CIMT in patients with subclinical hypothyroidism was analyzed. Results In 120 cases of subclinical hypothyroidism, there were 3 cases of gentleness type (2.50%), 30 cases of qi-depression type (25.00%), 28 cases of qi-deficiency type (23.33%), 25 cases of yang-deficiency type (20.83%), 13 cases of phlegm-damp type (10.84%), 9 cases of yin-deficiency type (7.50%), 6 cases of blood stasis type (5.00%), 5 cases of dampness heat type (4.17%), 1 case of particular type (0.83%). The main TCM constitutional types of subclinical hypothyroidism were qi-depression type, qi-deficiency type, yang-deficiency type and phlegm-damp type. The morbidity of atherosclerosis in patients with qi-depression type and qi-deficiency type were higher than other constitutional types. Conclusion The main TCM constitutional types of subclinical hypothyroidism were qi-depression type, qi-deficiency type, yang-deficiency type and phlegm-damp type.

PTH-related protein (PTHrP) is an abundant factor in bone which shows paracrine,endocrine,autocrine functions combined with PTHrP receptor and plays an important role in bone metabolism.Rencent years have witnessed that PTHrP plays a significant therapeutic effect on osteoporosis and exhibits some new functions.

Objective: To construct the miR-186 overexpression lentiviral vector and package the lentivirus, and to explore the infection efficiency and the expression level of miR-186 in the HEK293T cells. Methods: The Hsa-miR-186 precursor sequence was used as a template to design and synthesize the primer, and the the pre-miR-186 gene was amplified by PCR. The pre-miR-186 gene sequence was cloned into the lentiviral vector FV040 carrying EGFP/Puromycin cassette. The recombinant lentiviral vector was digested by EcoR I and Age I restriction endonuclease and confirmed by sequencing. The recombinant FV040 Vector and FV040 miR-186 were co-transfected into the HEK293T cells with the helper plasmids using Lipofectamine 2000, respectively; the FV040 Vector lentivirus (control group) and the FV040 miR-186 lentivirus (experiment group) were collected and used to infect the HEK293T cells 48 h after transfection, respectively. The green fluorescence distribution in the HEK 293T cells was observed 48 h after transfection, and the expression level of miR-186 was determined by real-time fluorescence quantitative PCR. Results: The sequencing analysis results indicated that the sequence of miR-186 overexpressing lentivirus was identical with the sequence of miR-186 published on GenBank. The titers in control group and experiment group were 6×108 TU · mL-1 and 5 × 108 TU · mL-1, respectively. The relative expression level of miR-186 in the HEK293T cells in experiment group (12. 640 0 ± 0. 788 4) was significantly increased by 15. 07 times (t=14. 72, P<0. 01) compared with control group (0. 838 7 ± 0. 145 6). Conclusion: The lentiviral vector which overexpresses miR-186 is constructed successfully and the miR-186 lentivirus is prepared. The HEK 293T cells are infected with miR-186 lentivirus successfully and the expression level of miR-186 in the HEK 293T cells is increased significantly.

Aim The goal is to make a further comprehension of the pathogenesis of Parkinsons disease (PD) and hence add further knowledge for PD diagnosis by observing the functions of nitric oxide (NO) in striatum (STR). Method Microelectrodes were used to observe the effects of sodium nitroprusside (SNP), L-glutamic acid (GLU) and ?-aminobutyric acid (GABA) on STR neurons' spontaneous firing rates, and at the same time, we observed the effects of NO on GLU and GABA. Results 77% (51/66) of the tested neurons were excited by SNP, NO synthase inhibitor L-NAME antagonized the excitatory effect of SNP. Duringthe periods of microelectrophoresis GLU and GABA, SNP amplified the excitatory effect of GLU and weakened the inhibitory effect of GABA. L-NAME antagonized the excitatory effect of GLU.Conclusion These results demonstrated clearly that NO, GLU and GABA functions might converge in the same STR neurons. NO and GLU functions are excitatory whereas GABA function is inhibitory on the firing activities in STR neurons.

Aim To investigate the vasodilatory effect and the mechanisms of urocortin(Ucn) on the thoracic aorta of spontaneously hypertensive rats(SHR).Methods Rings cut from SHR thoracic aorta were used in vitro.The endothelium dependent and independent vasorelaxing effects of Ucn were measured.Furthermore,it was also explored whether the relaxing effects of Ucn were affected by N~((?)) nitro-L-arginine methyl ester(L-NAME), methylene blue(MB) and glybenclamide.Results Ucn(1 nmol?L~(-1)～1 ?mol?L~(-1)) caused concentration dependent relaxation in SHR thoracic aorta with endothelium and without endothelium(P

Objective To compare the effects of combination of maternal hydration and hands and knees posture with simple hands and knees posture on correction of breech pregnancy. Methods One hundred patients who were diagnosed breech pregnancy from November 2015 to November 2016 were assigned to experimental group and control group with 50 cases each according to individual willingness. The patients in experimental group received the combination of maternal hydration and hands and knees posture;while the patients in control group received simple hands and knees posture. Results Amniotic fluid index was (18.94±2.44)cm in experimental group and (15.97±2.33)cm in control group, the difference had statistic significance (t=6.239, P<0.05). The effective transfer rate and successfully used time was 92%(46/50) , (2.10 ± 0.75) weeks in experimental group and 64%(32/50), (4.27 ± 0.98) weeks in control group, the difference had statistic significance (χ2=11.422, P<0.05; t=-12.463, P<0.05). For primipara and multipara, the effective transfer rate was 90.91%(30/33), 16/17 in experimental group, and 68.75%(22/32), 10/18 in control group, the difference had statistic significance (χ2=4.986, 6.806, all P<0.05). The rate of vaginal and cesarean section deliveries was 86%(43/50), 14%(7/50) in experimental group, and 56%(28/50), 44%(22/50) in control group, the difference had statistic significance(χ2=10.928, P=0.001). Patients who had fetal movement or who didn′t have time to do correction or missed was 17.10%(46/269), 1.49%(4/269) in experimental group, and 12.55% (22/255), 7.06% (18/255) in control group. Conclusions @Therapic effects of combination of maternal hydration and hands and knees posture is much better than that of the simple hands and knees posture therapy on correction of breech pregnancy, which is worthy of popularization and application in clinic.

BACKGROUND:Induced pluripotent stem cel technology have solved the contradiction between the ethics and immune rejection, and this high-efficient and safe technique is becoming the mainstream of today’s research. OBJECTIVE:To comprehensively review the safety and application of induced pluripotent stem cel s.METHODS:A computer-based online retrieval of PubMed and CNKI was performed to search relevant papers published from January 2006 to April 2016, with the key words of“induced pluripotent stem cel , reprogramming, clinical application, safety, transcription factor, disease mode”in English and Chinese, respectively. RESULTS AND CONCLUSION:In recent years, research on induced pluripotent stem cel s has attracted much attention from the scientific community and the medical community, and this technique has successful y gained induced pluripotent stem cel s and overcome the problems of immunity and ethics. However, it is limited to the theoretical and laboratory research due to the inability to solve the safety, efficiency and re-differentiation mechanism of induced pluripotent stem cel s. Therefore, we are faced with enormous difficulties and chal enges, which involve al aspects of basic research, including how to safely and effectively induce the differentiation of induced pluripotent stem cel s into the desired cel type and how to establish a suitable disease model as wel as a high-throughput drug screening platform.