Objective: To observe the safety and clinical efficacy of tumor antigen-pulsed dendritic cell(DC) vaccine in treatment of advanced malignant tumor.Methods: Ninety-one patients with non-small cell lung cancer,colon and rectal cancer,melanoma,renal carcinoma,breast cancer and other malignant tumors were enrolled in this study.All patients met the selecting standard and signed informed consent.Human dendritic cells were obtained from peripheral blood monocytes by culturing them with granulocyte macrophage-colony stimulating factor and interleukin-4.DC vaccine was prepared from tumor antigen pulsed immature dendritic cells in vitro.Patients received the vaccine therapy once every week and one cycle was defined as once every week for 3 weeks.Results: All the patients received 96 cycles of DC vaccine treatment.Symptoms of toxicity included fever,shivering,aching pain of muscle,asthenia,itching,stifle and transient fatigue;most of the symptoms automatically recovered.Clinical efficacy of the treatment was evaluated in 76 patients.Thirty-one of the 76 patients were stable after treatment and 45 were in progressive situation,with the clinical benefiting rate being 40.8%.Eighty-five patients were followed up.The median time for progression was 2.6 months;the overall survival time was 0.9-30.6 months;and the median survival period was 4.5 months,with the one year survival rate being 9.2%.Conclusion: The results suggest that the DC vaccine therapy is well tolerated in treating patients with advanced malignant tumors and has satisfactory clinical benefit;the clinical value of DC vaccine therapy needs to be further observed.

Objective To explore the community rehabilitation guidance for patients of vertebral osteoporosis fracture after percutaneous vertebroplasty. Methods The study collected 262 cases of osteoporotic fracture patients in community rehabilitation stage after PVP who leaved hospital from February 2010 to February 2014. Using the methods of drugs, diet, common exercise and systematic back muscle exercise. According to block random number method, the participants were divided into two groups: group A ( general guidance) and group B ( specialized guidance) . The incidence of the pain relapse and the fracture relapse would be observed. Results In group A, the incidence of pain relapse were 26 cases and fracture relapse were 6 cases; in group B, the incidence of pain relapse were 12 cases and fracture relapse were 5 cases. The differences of the incidence in pain relapse between two groups had statistical significance (χ2 =5. 54,P <0. 05), but the incidence of fracture relapse had no statistical difference (χ2 =0. 07,P>0. 05). Conclusions The community rehabilitation nursing is an important stage of holistic nursing mode and a guarantee to fully play the curative effect on the percutaneous vertebroplasty.