OBJECTIVE:To provide reference for rational use of recombinant human granulocyte-colony stimulating factor (rhG-CSF) in cancer patients. METHODS:Referring to the expert advice and guideline of clinical application of rhG-CSF at home and abroad,DUE criteria on rhG-CSF for cancer patients was established;questionnaire was designed,and the medical re-cords of rhG-CSF in a third grade class A hospital was evaluated. RESULTS:The DUE criterion on rhG-CSF for cancer patients included 3 parts,such as drug indications,the course of medication and medication results,14 items in total. Through the imple-mentation of DUE,retrospective evaluation of 220 medical records showed that the application of rhG-CSF was up to the stan-dard in respects of drug indications,no contraindication,routine blood test and drug interaction monitoring at least twice every week,there still was irrational phenomenon. CONCLUSIONS:The established DUE criterion on rhG-CSF for cancer patients is with the characteristics of definite content,target and paracticabilitg,which can provide reference for the work development of clinical pharmacists.

Lung cancer is the main cause of cancer-related death worldwide. Non-small cell lung cancer (NSCLC) accounts for about 80% of lung cancer cases, but only 25%-30% of initially diagnosed patients have the option of radical surgery because of the lack of effective measures for early diagnosis. For locally advanced and advanced NSCLC, radiotherapy alone or comprehensive treatment with chemoradiotherapy is the main treatment method; however, the curative effect is unsatisfactory. Recently, increasing evidence sug-gests that targeted drugs, such as epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), combined with radiotherapy/chemoradiotherapy represent a promising treatment modality for NSCLC. This review will discuss the research status of EGFR-TKIs and radiotherapy for locally advanced and advanced NSCLC.

Objective To study the application of census method in estimating the size of population of commercial sex workers(CSW),and estimatie the size of population in CSW in a city.Methods Drawing the map of the place which applied sex service,collect the number of CSW which the manager of place report.Sample 20% place of total,check the amount of all CSW,then adjust the sum of managers reported according the number of investigator checked and the number of manager reported to estimating the size of population in CSW in the city.Results The result of census in estimating the CSW in the city was 18 702.Conclusions It is simple and direct to estimate the size of population in CSW by applying the census method.The result is reliable,but the method need the investigators know very well the palce applied sex service in the area.

Objective To study the application of Capture-Recapture method in estimating the size of population of injecting drug users (IDUs), and estimate the size of population in IDUs in a city using the data of detoxification center. Methods Capture-Recapture method was applied to estimating the size of population in IDUs in the city based on the data of detoxification center. Results The result of Capture-Recapture in estimating the IDUs in the city was 13 017. Conclusions It is economic and feasible to estimate the size of population in IDUs by applying the Capture-Recapture method. The result is reliable and the method is suitable in districts where health resources are limited.

Background and purpose: The ataxia-telangiectasia mutated (ATM) gene results in ataxia-telangiectasia (A-T) and it is closely associated with tumors. ATM is an important signal transducer that is involved in the repair of DNA double-strand break damage by phosphorylating numerous target proteins . This study was aimed to investigate the correlation between a single nucleotide polymorphism (SNP) in ATM gene (IVS62+60G>A) and the risk of non-small cell lung cancer(NSCLC) in a case-control study. Methods: From June 2004 to December 2005, a total of 264 patients with NSCLC were recruited, 264 healthy people as control. All of specimens were collected from Zhejiang Tumor Hospital. DNA was extracted from peripheral blood and then was used to determine. ATM genotype by Taqman SNP genotyping assays. Logistic regression model was employed to analyze the relationship between SNP and NSCLC risk. Results: The percentage of NSCLC patients in 86 patients with A/A genotype, 139 patients with A/G and 39 patients with G/G were 32.6% (86/264), 52.6% (139/264), 14.8% (39/264), respectively. The percentage in 68 healthy people with A/A genotype, 139 healthy people with NG and 55 healthy people with G/G were 26.0% (68/262), 53.0% (139/262) and 21.0% (55/262), respectively. The proportion of G/G genotype in 264 patients was obviously lower than that in the 264 healthy control (14.8% vs 21.2%, P<0.05). The people with G/G genotype had lower risk to NSCLC than there with A/A genotype (OR=0.561, 95% CI=0.334-0.942, P=0.029). Conclusion: The ATM SNP(IVS62+60G>A)was associated with the NSCLC risk, and homozygous G alleles may be a protective factor to NSCLC.

Objective: To establish the rational drug use standard for elderly hypertensive patients after comprehensive assessment, so as to provide evidence for rational drug use in clinics. Methods: Such standards as Beers, STOPP and START were summarized, key items were extracted,duplicate contents were deleted,and the initial standard was formed and used as the first round questionnaire. The Delphi method was used to conduct the second round expert consultation questionnaire,and the positive coefficients, authority and coordination degree of the experts were evaluated. Results: A total of 10 experts participated in the consultation, and the effective re-covery rate of the second round was 100% and the average authority coefficient was 0. 88. The coordination coefficient of the two rounds was 0. 57 and 0. 76, respectively. The standard of rational drug use for the elderly patients with hypertension after comprehensive as-sessment was finally completed. The standard contained 13 comprehensive assessment contents and 21 drug warning entries. Conclu-sion: After 2 rounds of expert consultation,the opinions tend to be uniform with high degree of coordination. The standard can provide reference for clinicians and clinical pharmacists to use drugs rationally. At the same time,it also provides ideas for the establishment of rational drug use standards for other diseases.

Objective To investigate the association between the osteogenic differentiation in the soft tissue lump and the clinicopathological characteristics of osteosarcoma patients. Methods We retrospectively reviewed the clinical data of conventional osteosarcoma patients with soft tissue lumps, including Enneking stages, chemotherapy sensitivity, overall survival and post-metastatic survival time. The ossification level in soft tissue lumps was assessed by imaging and the proportion of osteoid matrix was assessed by pathological examination. Results A total of 189 cases were included in this study. In patients with Enneking IIIB, non-osteoblastic, partially osteoblastic and osteoblastic types accounted for 30.2%, 9.6% and 6.3%, respectively. Non-osteoblastic osteosarcoma patients had a higher rate of initial metastasis (P < 0.05); Chemotherapy efficiency of non-osteoblastic, partially osteoblastic and osteoblastic types were 60.5%, 59.6% and 31.3%, respectively. The osteoblastic osteosarcoma held the worst rate of chemotherapy sensitivity (P < 0.05). The overall survival of non-osteoblastic osteosarcoma was shorter than those of partially osteoblastic and osteoblastic types (P < 0.05). Post-metastatic survival time of osteoblastic osteosarcoma was longer than that of non-osteoblastic osteosarcoma (P=0.078). Conclusion For conventional osteosarcoma, the osteogenesis level in soft tissue lumps is related to the surgical stage, chemotherapy sensitivity and prognosis of tumors, which may provide guidance for the individual decision regarding chemotherapy and surgery timing on patients.

Objective In May 2013,an abnormal increase of surgical-site infection among post cesarean section patients was reported at one hospital in Zhongshan. An investigation was conducted to identify the risk factors and related control measures. Methods All the reported surgical-site infection records among post cesarean section patients were checked. A review of cesarean section schedules of health workers was also performed. An 1∶2 case-control study was conducted among surgical-site infection cases in May 2013. Microbiologic cultures were performed on 2 surgical site secretion samples and 12 samples from the environment. All the positive isolates were molecular typed by pulsed field gel electrophoresis(PFGE). Results In May 2013,4 post cesarean section patients who developed surgical-site infection symptom at one hospital in Zhongshan were reported,with an attack rate as 10.3%(4/39). The emergence time of symptom was 2-3 days after operation. All of the 4 cases underwent an emergency operation. The median time interval for cases from admission to operation was 7.2 hours(ranged from 2 to 9 hours),lower than that seen in the controls,with a median time of 20.8 hours (Z=5.50,P=0.03). Two of the 4 cases took type-B ultrasonic inspection 1.4 h and 8.4 h before the operation,and the other two cases took continuous fetal heart monitoring 2 hours before the operation. Skin of the operation area on the 4 cases had been exposed to ultrasonic couplant,without a thorough clean. The proportion of type-B ultrasonic inspection or continuous fetal heart monitoring was much higher in cases than in controls(χ2=5.19, P=0.01). Burkholderia cepacia (BC) isolates were discovered from:one surgical site secretion,2 type-B ultrasonic probe samples,one ultrasonic couplant in use and one ultrasonic couplant unopened. All the isolates were identified as 100%identical by PFGE. Conclusion The skin of operation area of cesarean section patients had been exposed to BC contaminated ultrasonic couplant without thorough cleaning,which seemed to be related to the outbreak of surgical-site infection,in our case.

Radiotherapy is a critical approach for the comprehensive treatment of non-small cell lung cancer.Deep understanding of the individualized radiosensitivity of lung cancer patients plays a pivotal role in the selection of radiotherapy dosage and regime and establishment of comprehensive therapeutic strategies.Currently,multiple researchers have identified a variety of biomarkers in predicting the radiosensitivity of lung cancer patients.In this article,research progress on the biomarkers in predicting radiosensitivity of non-small cell lung cancer was reviewed.

ObjectiveTo explore the component factors of anhedonia in first-episode schizophrenia patients and the relationship with clinical symptoms， cognitive and social functioning. MethodsA total of 31 patients with first-episode schizophrenia who met the diagnostic criteria of the International Classification of Diseases， tenth edition （ICD-10） and another 33 healthy controls were enrolled. Then， the anhedonia level， mental symptoms， cognitive and social functioning were assessed using Temporal Experience of Pleasure Scale （TEPS）， Positive and Negative Syndrome Scale （PANSS）， Repeatable Battery for the Assessment of Neuropsychological Status （RBANS） and Personal and Social Performance Scale （PSP）. Thereafter， Pearson correlation was used to discuss the correlation of anhedonia level with clinical symptoms， cognitive and social functioning. ResultsThe consummatory anhedonia score in TEPS of first-episode schizophrenia patients was lower than that of healthy controls， with statistical difference ［（27.71±5.48） vs. （31.58±5.92）， t=2.705， P=0.009］. Correlation analysis showed that consummatory anhedonia had no correlation with PANSS， RBANS and PSP scores in first-episode schizophrenia patients（P>0.05）. ConclusionFirst-episode schizophrenia patients have consummatory anhedonia， and the consummatory anhedonia may be independent of clinical symptoms， cognitive and social functioning.