Objective To discuss expression of livin genes in colon cancer tissues chip and its correlation with Bcl-2 genes. Methods A total of 60 cases of patients with colon cancer, who chose to be treated with surgical operation in General Surgery Department were selected, and applied with immunohistochemical MaxVisionTM two step method to detect the expression of livin and Bcl-2 genesn in tissues chip. 25 cases of colon tissues chip with normal cutting edge were additionally chosen as control group. Results Positive livin genes expression rates in colon cancer tissues chip (63.33%) were much higher than those in colon tissues with normal cutting edge, which appeared statistical differences (24.00%) (&chi;2=10.93, P<0.01). Livin genes expression in colon cancer tissues chip had no obvious relevance with gen-der, age, tumor size, differentiation degree, lymphatic metastasis and other pathological characteristics (P>0.05), but ob-vious relevance with invasive depth, Duke’s stages, distant metastasis and other pathological characteristics (P<0.05 or P<0.01). Positive Bcl-2 genes expression rates in colon cancer tissues chip (68.33%)were much higher than those in colon tissues with normal cutting edge, which appeared statistical differences (4.00%) (&chi;2=29.22, P<0.01). Livin genes expres-sion was positively relatedto Bcl-2 genes in expression in colon cancer tissues chip (P<0.01). The invasive depth, Duke’s stages and distant metastasis were included in meta stasis of dinthe Logistic regression model for livin genes expression (P<0.05). Conclusion Livin genes play important roles in the disease process of colon cancer tissues chip and have close relevance with pathologic process of malignant invasion and transfer of tumor. The expression of Livin genes and Bcl-2 genes may play synergetic roles in process of colon cancer.

Objective To discuss curative effect of somatostatin on acute adhesive intestinal obstruction and its protec-tion effect on intestinal mucous membrane barrier function. Methods All 90 cases of patients with acute adhesive in-testinal obstruction were divided into two groups, with 45 cases of patients in research group and control group respec-tively. The patients in control group were given routine medical treatment, such like fasting, water-fast, continuous gas-trointestinal decompression, antibiotics to prevent infection, relieving spasm and pain, adjustment of water-electrolyte imbalance, total parenteral alimentation(TPA)and etc. The patients in research group were additionally given 3 mg so-matostatin injection, 50 mL normal saline(NS) with minipump by 4 to 6 mL per hour, until cacation or exhaust from anus, given transit operation treatment or termination of treatment, while the patients in control group were given the same medical treatment as that in research group except for somatostatin. The changes of serum endotoxin, diamineoxi-dase (DAO)and procalcitonin(PCT)levels of patients in two groups before and 5 days after medical treatment were ob-served and compared, and the clinical curative effect and untoward effect were evaluated as well. Results After 5 days'medical treatment, the serum endotoxin, DAO and PCT levels of patients in two groups were obviously declined than before (P<0.05 or P<0.01), and the declining rate of patients in research group was much higher than that in control group(P<0.05), and meanwhile, the total clinical efficiency of patients in research group(95.56%)was much higher than that in control group(80.00%)(&chi;2=5.07, P<0.05). 3 cases(6.67%)and 5 cases(11.11%)of untoward effect were appeared on patients in control group and research group during treatment, and no statistical differences were appeared after comparison(&chi;2=0.14, P>0.05). Conclusion The somatostatin has reliable curative effect on adhesive intestinal ob-struction after abdominal operation, and can obviously improve clinical symptom with high security, whose mechanism of action may have close effect on reducing serum endotoxin, DAO and PCT levels and protecting and repairing in-testinal mucous membrane barrier function.