The A/G polymorphism of cytotoxic T lymphocyte associated antiger 4(CTLA-4) was determined by PCR-RFLP in 120 patients with recurrent Graves′ disease (GD), including 85 cases with early relapses within 3 years and 35 cases with relapses more than 3 years after withdrawal of antithyroid medication.66 initial GD patients and 100 healthy controls were included in the present study.The results suggest that A/G polymorphism of CTLA-4 was associated not only with GD, but also with early relapes after withdrawal of antithyroid medication.

OBJECTIVE:To promote the rational use of Chinese patent drugs(CPD).METHODS:The efficacy of several groups of CPD which share the similar formulation were compared and the components in each formulation were further analyzed in detail.RESULTS:The origins and pathogenesis of diseases treated with different CPD of the similar formulation varied,so did their indications.CONCLUSION:Only by following the principle of"Syndrome Differentiation Treatment"can the efficacy of CPD be maximized.

Objective To solve the problem of erectile dysfunction in clinical forensic medicine investigation by using the nocturnal penile tumescence (NPT) testing. Method 13 cases who complained erectile dysfunction after trauma were studied by using RIGISCAN PLUS SYSTEM. Results In 13 cases, the results of NPT testing showed that the erectile function was normal in 3 cases, slight dropping in 5 cases, medium dropping in 2 cases, and completely losing in 3 cases. In 5 cases which were serious erectile dysfunction as tested by IIEF, the NPT showed that the erectile function was normal in 1 case, slight dropping in 1 case, medium dropping in 1 cases, and losing completely in 2 cases. Conclusion The nocturnal penile tumescence testing can solve the problem of erectile dysfunction in forensic medicine investigation including the estimatieon of the severity of injury, labor ability assessment and some related questions.

The -1031T/C polymorphism of tumor necrosis factor-? (TNF-?) gene was determined by PCR-RFLP in 54 patients with thyroid-associated ophthalmorpathy (TAO), 60 patients with autoimmune thyroid disease (AITD) without ophthalmopathy and 76 healthy subjects. The results showed that the frequencies of TC+CC genotype and C allele in TAO group, especially in male patients, were significantly higher than those in the other two groups (both P

Objective To explore the relationship between calcium channel ?1 subunit (Cav1.1) gene intron 26 -67 A/G polymorphism and thyrotoxic periodic paralysis(TPP). Methods Cav1.1 gene polymorphism at position -67 was determined by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) in 46 male patients with TPP, 68 male Graves’ disease (GD) patients without TPP and 72 healthy male controls. The difference of genotype and the variation of allele frequencies were analyzed by Chi-square test. Results (1) Frequencies distribution of AG+GG genotype in TPP, GD and control groups were 47.83%, 14.71% and 29.17% respectively, and those of allele G were 44.57%, 13.24% and 27.78% for the three groups respectively. (2) Frequencies of -67 AG+GG genotype in TPP group were significantly higher than those in GD and CON group(OR=5.32, P

OBJECTIVE:To inverstigate the inhibitory effects of 14 kinds of traditional Chinese medicines on tyrosinase activity METHODS:The effective components of traditional Chinese medicines were extracted by 50% ethanol The speed of enzymic catalysis of tyrosinase was determined by ultraviolet spectrophotometry to evaluate the inhibitory effects of extracts of traditional Chinese medicines RESULTS:14 reactive time curves of enzymic catalysis of tyrosinase were obtained CONCLUS_ION:13 kinds of traditional Chinese medicines in vitro showed a better inhibitive effect on tyrosinase activity(P

The G395R mutation of human sodium/iodide symporter gene was investigated by PCR-RFLP in 52 children with congenital hypothyroidism and 106 health children. The result suggested that G395R mutation may not be the main cause of congenital hypothyroidism in Qingdao.

Objective To explore the sleep problem and its related factors,and to provide evidence for improving sleep quality of patients with ankylosing spondylitis (AS). Methods Pittsburgh sleeping quality index (PSQI),self-rating depression scale (SDS),self-rating anxiety scale (SAS) and a self-designed questionnaire were applied to survey 318 patients with AS.Data were analyzed by the SPSS 17.0 software.The relationship between the various relevant factors were revealed by t-test,x2 test,Wilcoxon rank-sum test and Pearson correlation analysis.Results The mean total score of PSQI was 6.6±3.6, and 35.4％ of AS outpatients showed poor sleep quality.The difference of PSQI scores and detection rate of poor sleep quality between male and female were not statistically significant.There were significant difference of early morning stiffness(Z=-3.077,P=0.002),BASFI(Z=-4.766,P=0.000),BASDAI(Z=-6.880,P=0.000),nocturnal pain VAS(VAS)(Z=-6.502,P=0.000),total back pain VAS(Z=-6.675,P=0.000),ESR(Z=-2.370,P=0.018),CRP(Z=-2.063,P=0.039) between the poor sleep and the good sleep patients (P＜0.05).The PSQI score was positively correlated with age (r=0.165,P=0.003) but not gender and disease duration (r=0.078,P=0.165; r=0.094,P=0.097).PSQI score difference were also observed between patients with negative mood and patients without negative mood (t=-7.613,P=0.000; t=-7.287,P=0.000).Conclusion High incidence of sleep disorder exists among AS outpatients,and it is closely related with age,anxiety,depression and activity of AS.Attention should be paid to AS patients with sleep problems.

Basic biochemical index were measured weekly in hyperuricemia rats.Real-time quantitative PCR technology was employed to measure glucose transporter-4 (GLUT-4),insulin receptor substrate (IRS)-1,and IRS-2 mRNA expression in the skeletal muscle and adipose tissue.The gene expression of GLUT-4 and IRS-2 are significantly lower in the last weeks of the hyperuricemia rat.Insulin resistance caused by hyperuricemia is probably related to the lowering expression levels of GLUT-4 and IRS-2 mRNA in the target tissues.

Objective To elucidate the relationship between serum uric acid (SUA) and cardiovascular risk factors in Shandong coastal inhabitants. Methods A random, stratified cluster sampling method was conducted. A population.based cross-sectional survey for hyperuricemia was performed and 4 988 dwellers of Shandong coast were investigated. Fasting SUA,lipid profiles,insulin concentrations,etc.,were determined by automatic biochemical analysis device and electrochemiluminescence analyzer.The investigation of general,healthy and nutritional conditions was carried out by questionnaire. Results In male and female subjects, the cardiovascular risk factors such as systolic blood pressure,diastolic blood pressure, selum ereatinine,total cholesterol.triglvceride,waist circumference and body mass index all increased with rising SUA,and the level of senlm high densitv lipoprotein fell accordingly.Homeostasis model assessment insulin resistance index (HOMA- IR) in female subjects increased by the quartile group,not in male subjects.Compared with SUA<280 μmol/L group.odds ratio(OR) values of hypertension in SUA 280-319,320-349,≥350 μmol/L groups were 1.54,1.88 and 2.70 respectively in female,and 2.29,4.15 and 4.96 for metabolic syndrome (MS).As for male,compared with SUA<280 μmol/L group OR value of hypertension in SUA ≥420 μmol/L group was 1.52,and OR values of MS of 1.41,1.87 and 3.27 occurred in SUA 320～379,380～419,≥420 μmol/L groups.After being adjusted for confounding factors,logistic stepwise regression revealed that elevated SUA was an independent risk factor for hypertension in both genders.Conclusion Elevated SUA level is closely related to the incidence and development of cardiovascular diseases,and should not be ignored in clinical practice.