【Objective】To investigate the effect of serum containing Yifa Compound(YC) on the growth of hair papilla cells.【Methods】Hair papilla cells were cultured in vitro.After subculture for 4～6 generations,hair papilla cells at logarithmic growth phase were cultured with YC-containing serum(at the concentrations of 1.25,2.5 and 5.0?g/L respectively) and blank serum respectively.After the culturing,the proliferation curve of hair papilla cells was drafted by methylthiazolyltetrazolium(MTT) assay and their growth was observed under scanning electron microscope.【Results】Optical density of hair papilla cells was higher at logarithmic growth phase while lower at growth lag phase in high-dose YC-containing serum group than that in blank serum group.Moderate-and high-dose YC-containing serum promoted the agglutinating radial growth.【Conclusion】Serum containing YC can promote the proliferation of hair papilla cells.

Objective Study of the feasibility that the former changing trend of tumour after the first BAI can forecast the later curative effect on tumour.Methods Bronchus artery and relational body arteries opacification and BAI have been introduced in 82 primary pulmonary carcinoma cases.In the statistic analysis,SPSS 13.0 for Windows Stat.Software Package has been used.At the meanwhile chi square test,one-sample t-test,GLM-univariate analysis have been adopted as well to study the relationship between the former changing trend of tumour and Later Curative Effect after BAI in mid-advanced stage pulmonary carcinoma.Result There are significance statistical significances between the former changing trend of tumour and the later curative effect after the first BAI in mid-advanced stage pulmonary carcinoma.Conclusion The former changing trend of tumour after the first BAI is the factor influencing the later curative effect.

Recently diverse magnetic resonance imaging technology is used in glioma grading,which based on the theory of Gaussian distribution.Diffusion kurtosis imaging emerges as an extension of DWI and DTI,which could reflect the real and actual hydron non-Gaussion distribution of tumor and peritumoral microenvironment.Displaying the correlation with immunohistochemical label Ki-67,DKI convinces more scholars inland and abroad with its practicability.

Objective To study later eurative effects after BAI with single factor analysis.MethodsBronchus artery and relational body arteries opacification and BAI had been introduced in 82 primary pulmonary carcinoma cases.In the statistic analysis,SPSS 13.0 had been used.ResultsThe tumour blood supply types and the times of effective BAI treatment were the factors influencing the later treatment period.Tumour pathologic types,the branches of tumour blood supply,tumour clinic cTNM stages,chems project influenced faintly later curative effect.ConclusionThe blood supply types of primary pulmonary carcinoma and the times of effective BAI treatment are the factors influencing the later treatment period.

Objective To investigate prenatal ultrasound diagnosis of clubfoot and its clinical value.Methods Three thousands pregants ranging gestational age from 12 through 40 weeks were checked using Ascuson Sequoia 512 equipment.The diagnosis of clubfoot was made prospectively at the time of the scan by the following criteria: the ball of foot had to be visible in the same plane as the longitudinal section of bones of the lower leg,unchanging with fetal activity and reproducible for several images.All prenatal ultrasonographic dignoses of clubfoot were proved by autopsy or newborn physical examination.Results A total of 17 cases clubfoot fetuses were prenatal diagnosed by ultrasonography.Fourteen of 17 clubfoot fetuses had associated with complex abnormalities,three of them were isolated clubfoot.Fourteen of 17 cases with clubfoot prenatal diagnosed by ultrasound were proved by autopsy.Another three cases with clubfoot were proved by newborn physical examination,two of them were false positive diagnosis.Conclusions Prenatal diagnosis of clubfoot is reliable by ultrasound.It is helpful in diagnosis with other structural abnormalities associated with clubfoot.It can also provide direction for clinical obstetrical management.

Objective To investigate prenatal ultrasound diagnosis of radial ray abnormalities and its clinical value. Methods All the fetus that entered usual prenatal ultrasound diagnosis were scanned each limb using a systematic continuous sequence approach (SCSA) with Acuson Sequoia 512 and GE Logiq 400 equipments. All the artificial aborted fetal radius malformation also had ultrasound examination in a simulating intrauterine estate and /or X-ray examination. Results Correctly diagnosis was made in 13 cases fetal radial ray abnormalities by prenatal ultrasonography following SCSA through the scaning course. All the fetal radial ray abnormalities had radius absence(only one skeleton in the forearm) or radius dysplasia and characteristic radial club hand. Ray abnormalities can be classifyied into three types on ultrasonography: type Ⅰ, radius absence; type Ⅱ,radius part absence; type Ⅲ,radius dysplasia.Conclusions Mastering characteristic radial club hand and SCSA are the keys to diagnose fetal radial ray abnormalities by prenatal ultrasonography.

Objective To improve the recognition of dermatomyositis (DM) complicated with intracranial neoplasma and to explore its pathogenesis aimed to collect the experience of diagnosis and treatment. Methods Twelve DM patients complicated with intracranial neoplasma were analysed and the literature of recent 23 years were reviewed. Results DM complicated with intracranial neoplasma was associated with the p53 genic mutation and it was rare because of blood-brain barrier and scanty of lymphocytes in brain tissue. Correct diagnosis depended on clinical manifestations and the computerized tomography or the magnetic resonance imaging of brain. Glucocorticoid was the first choice treatment.However, removal the neoplasma was necessary. Conclusion DM complicated with intracranial neoplasma is rare. Clinicians should make the diagnosis promptly and chosen the appropriate treatment.

Objective To investigate the diagnostic values of color ultrasound to the stenosis of extracranial carotid arteries.Methods Color ultrasound examination and digital subtraction angiography(DSA)were performed in 145 patients with ischemic cerebravascular disease.The sensitivity and specificity of color ultrasound for the stenosis of extracranial carotid arteries were analyzed according to the golden standard of DSA results.Results The sensitivity and specificity of color ultrasound for stenosis of carotid bifurcation,subclavicular artery and vertebral artery were 96.8%,80.6%,60.0% and 98.7%,33.3%,89.0%.The accuracy of color ultrasound for stenosis of carotid bifurcation,subclavicular artery and vertebral artery were 93.0%,92.8% and 82.1%.Conclusion Color ultrasound examination are effective for the diagnosis of carotid bifurcation sterosis,and less effective for the diagnosis of the subclavicular and vertebral artery stenosis because of the poor sensitivity.

Objective To identify the clinical features of patients with brucellosis in Liaoning Province in recent 5 years, and to improve the diagnostic level of the disease.Methods The clinical data including epidemiology, clinical features, laboratory data and diagnosis of 82 hospitalized patients who were diagnosed with brucellosis in the First Affiliated Hospital of China Medical University from 2011 to 2015 were collected and reviewed retrospectively.Results The majority of the 82 patients were middle-aged (45-59 years old) (47.6%) and male gender (63.4%).Fever (90.2%), muscle and joint pain (61.0%), hepatosplenomegaly (36.6%), lymphadenopathy (24.4%) and weight lose (32.9%) were the main complaints.The peripheral leucocyte counts were usually normal (70.7%).C-reactive protein (81.3%), procalcitonin (81.8%) and erythrocyte sedimentation rate (64.2%) increased in most cases.The results of the serum agglutination test in 67 patients were all positive (100.0%).The pathogen isolation was conducted in 60 patients and 49 patients were positive for Brucella species.Among them, 35(71.3%) isolates were identified as Brucella melitensis.A total of 90.2%(74/82) cases were firstly diagnosed with fever of unknown origin and were hospitalized.Misdiagnosis accounted for 52.4(43/82)%.Conclusions Contact history with cow and sheep should be inquired thoroughly in patients with clinical manifestations of fever, muscle and joint pain and hepatosplenomegaly.Pathogen isolation, agglutination test and other specific tests should be performed as soon as possible to diagnose the disease early.

Objective To improve the understanding of the vertebrobasilar dolichoectasia (VBD) by analyzing the MRA imaging features of VBD.Methods The data of 438 patients underwent MRA were analyzed in the study.The length and transverse deviation of the basilar arteries (BA) were measured for the diagnosis and classification of VBD.Of 438 patients, 342 patients were diagnosed as brain infarction (BI) by head MRI.All of the BI patients were divided into 2 groups (VBD and non-VBD) and the classifications of BI were compared between the two groups.Results Of 438 patients, 16 were diagnosed with VBD by MRA.According to the modality of VBD on MRA, VBD were classified into 4 types: S type, U type, L type and spiral type.Among the 16 patients, there were 7 patients with S type, 5 patients with U type, 3 patients with L type and 1 patient with spiral type.S type had the highest incidence and the mildest symptom.The incidence of VBD in BI patients was 3.98%, and the incidence of BI in VBD patients was 2.1 times greater than that of patients without VBD (P<0.05).Conclusion VBD can be classified into 4 types according to the MRA results.The clinical symptoms are varied with different imaging features, and VBD is closely related to the posterior circulation ischemia, especially brainstem infarction.