Objective To discuss the influence of Danshen Chuanxiongqin injection on renal function and relevant indexes of urine in rats with sepsis -induced acute kidney injury(AKI))caused by cecal ligation and punc-ture (CLP).Methods Seventy male Sprague Dawley(SD)rats were randomly divided by random number table into normal group,sham -operation group,sepsis group and Danshen Chuanxiongqin group.The three latter groups were divided into groups of 6,12,24,48 -hour subgroups,five rats in each subgroup.Danshen Chuanxiongqin group was injected with Danshen Chuanxiongqin injection at 0,24,48h from caudal vein seperately,and the other groups were injected with saline solution.The blood and urine were collected in the set four time points.The relevant indexes like serum creatinine (SCr),blood urea nitrogen(BUN),IL -6 and TNF -αwere tested and kidney injury molecule -1 (KIM-1)and neutrophil gelatinase associated lipocalin (NGAL)were detected.Changes of renal pathology were observed under light microscope.Results In the sepsis group,the concentrations of SCr and BUN 6h after CLP star-ted to rise,reached peak in 24h to (96.31 ±12.64)μmol/L and (27.17 ±4.98)mmol/L,and decreased in 48 h, while concentrations of IL -6 and TNF -αwere reached peak in 12h (28.45 ±3.22)pg/mL and 6h (72.35 ± 10.84)pg/mL respectively,and then decreased,which were all higher than those in the normal group and sham -operation group (P <0.05).After Danshen Chuanxiongqin injection therapy,the concentrations of Scr,BUN,IL -6 and TNF -αin 6h,12h,24h and 48 h were all lower than those in the corresponding points in time in the sepsis mod-el group,and reached minimum values[(14.89 ±2.41)μmol/L,(17.29 ±3.37)mmol/L,(14.89 ±2.41)pg/mL, (17.29 ±3.37)pg/mL,all P <0.05].The contents of KIM-1 and NGAL 6 h after CLP started to increase,reached peak in 24h (5.47 ±0.39)ng/mL and 12h (6.35 ±0.81)ng/mL respectively,and decreased in the sepsis group were all remarkably higher than those in the normal group and sham -operation group.After given Danshen Chuanx-iongqin injection,the contents of KIM-1 and NGAL in 6h,12h,24h and 48h were greatly lower than those in the corresponding points in time in the sepsis group,reached minimum values[(2.27 ±0.16)ng/mL,(2.03 ±0.27)ng/mL,all P <0.05)].In the Danshen Chuanxiongqin group,the change of renal pathology was better than that of the sepsis group in 12h.Conclusion Danshen Chuanxiongqin injection plays the role of renal protection in AKI caused by sepsis.

Our previous study found that plate factor-4 variant (CXCL4L1) was downregulated in the serum of patients with prostate cancer (PCa). The aim of the present study was to investigate the prognostic value of CXCL4L1 in PCa. In total, 213 PCa patients treated with radical prostatectomy were enrolled and peripheral blood samples of all patients were collected. Expression of serum CXCL4L1 in patients with different tumor stages and grades were measured by enzyme-linked immunosorbent assay (ELISA). The Kaplan-Meier method was applied to estimate the progression to castration-resistant prostate cancer (CRPC), metastasis, biochemical recurrence (BCR)-free survival, and overall survival (OS). Prognostic factors for BCR-free survival and OS were determined by univariate and multivariate analyses using the Cox proportional hazards regression model. The expression of CXCL4L1 was significantly lower in PCa patients with advanced pathological tumor stage, high-grade Gleason score, and metastasis. Moreover, downregulation of CXCL4L1 not only strongly correlated with aggressive clinicopathological features, but also predicted tumor progression and unfavorable outcomes. Finally, multivariate Cox regression analyses identified CXCL4L1 as an independent prognostic factor for both BCR-free survival (hazard ratio [HR]: 2.03, 95% confidence interval [CI]: 1.26-3.27; P = 0.004) and OS (HR: 2.26, 95% CI: 1.07-4.79; P = 0.033). In conclusion, our results indicate that CXCL4L1 might serve as a novel and promising prognostic biomarker for patients with PCa and potential therapeutic target in the future.

Objective: To explore the correlation between ADC value of cerebral nuclei and age in patients with Alzheimer disease (AD) with different ages. Methods: Thirty AD patients (AD group) and 30 matched volunteers (control group) were enrolled and divided into 6 subgroups according to age (55-59 years old [n=3], 60-64 years old [n=4], 65-70 years old [n=9], 71-74 years old [n=5], 75-80 years old [n=6], >80 years old [n=3]). ADC values of left and right hippocampus, red nucleus, caudate nucleus, amygdala and putamen were measured. The paired t-test, independent sample t-test, one-way ANOVA and Pearson correlation analysis were performed. Results: There were significant differences of ADC values between left and right red nucleus in AD group (P=0.022), also of ADC values in right hippocampus, bilateral caudate nucleus and right caudate nucleus in different age subgroups of AD group (all P<0.05). There were significant differences of ADC values in bilateral hippocampus, putamen and caudate nucleus among different subgroups of AD group and control group (all P<0.05). Positive correlations of age and right hippocampus (r=0.615, P<0.001), right putamen (r=0.653, P=0.001) and bilateral caudate nucleus (left: r=0.397, P=0.030; right: r=0.429, P=0.020) were found in AD group. Conclusion: ADC values in bilateral hippocampus, putamen and caudate nucleus of AD patients increase with age. ADC values can provide references for clinical prediction and early diagnosis of neurodegenerative lesions in bilateral hippocampus, putamen and caudate nucleus of AD brain.

Alzheimer disease (AD) is the most common degenerative disease of central nervous system. Animal models can simulate the pathological changes of early AD. MRI is an important method for the diagnosis of AD. 7.0T small animal MRI promotes the study of AD. Multimodal imaging provides help for the diagnosis of early AD. The application of 7.0T small animal MRI technologies in AD research were reviewed in this paper.

Periodontitis is a chronic infectious disease in which periodontal tissue loss is caused by dental plaque biofilm. Atherosclerosis is a chronic inflammatory disease that occurs in the walls of arteries and is characterized by lipid accumulation. Recently, many studies have suggested that there is a certain relationship between periodontitis and atherosclerosis. From an epidemiological perspective, a previous literature review indicated that patients with periodontitis have a higher incidence of atherosclerosis. IL-17 secreted by Th17 cells may aggravate the progression of the two diseases by elevating the levels of matrix metalloproteinases, which may damage the connective tissue. Treg cells reduce the activation of T cells and limit the development of inflammation by secreting anti-inflammatory factors and expressing coinhibitory molecules. Periodontal intervention may contribute to the treatment of atherosclerosis by reducing inflammatory markers in atherosclerosis. Many studies have shown that periodontitis and atherosclerosis may interact with each other, but further studies are needed to explore the concrete mechanism of the interaction between periodontitis and atherosclerosis.

A large number of studies have confirmed that periodontitis is closely related to systemic multisystem diseases. Local inflammation of periodontal tissue may cause systemic inflammation. As an inflammatory factor, C-reactive protein (CRP) is widely used to monitor various inflammatory states. CRP is not only highly related to periodontitis but also closely related to systemic diseases. This article reviews the relationship between periodontitis and systemic disease epidemiology as well as the mechanism of CRP in the relationship between them. The literature review shows that periodontitis is closely related to cardiovascular diseases, diabetes, premature and low-birth-weight infants, chronic kidney disease, rheumatoid arthritis and other systemic diseases. Periodontitis, as a chronic inflammatory disease, can increase the level of serum CRP, thus increasing the risk of cardiovascular disease and affecting blood glucose control in diabetic patients, which may be related to the increased risk of premature delivery and low-birth-weight infants, may affect the development of chronic kidney disease, and may be used as one of the sensitive indicators of rheumatoid arthritis in the active stage. Through periodontal treatment, serum CRP levels can be reduced, which is helpful for the treatment of systemic diseases. CRP levels can be used as indicator for prevention, diagnosis and treatment of periodontitis and systemic diseases.

Objective : To explore the etiology, clinical manifestations, diagnosis, differential diagnosis and treatment of idiopathic gingival fibromatosis, and to provide references for clinical diagnosis and treatment. Methods : The clinical data and related literatures of a case of idiopathicgingival fibroma that occurred in the oral cavity were retrospectively analyzed. Results : Total periodontal treatment was performed for the patient, and the gingival morphology was improved after periodontal surgery in the anterior region. Idiopathic gingival fibromatosis is a rare disease characterized by gingival tissue hyperplasia. The etiology and pathogenesis are unknown. The disease can occur in young children. Generally, it occurs after the permanent teeth erupt, and it manifests as extensive gingival hyperplasia, which can affect the entire gingival margin, gingival papilla and attached gingival, and can even reach the membrane-gingival junction. The pathological changes include thickening of the spinous layer of the gingival epithelium, significant increases in the epithelial styloid process, increases in the connective tissue volume, and filling with large collagen fiber bundles and a large number of fibroblasts. The blood vessels are relatively small, and inflammation is not obvious. Clinically, this disease needs to be distinguished from drug-induced gingival hyperplasia and chronic gingivitis with hyperplasia as the main manifestation. At present, the treatment of idiopathic gingival fibromatosis is mainly gingival angioplasty. The disease easily relapses after surgery. The recurrence rate is related to the quality of oral hygiene. After recurrence, it can be treated again. Conclusion Idiopathic gingival fibromatosis is relatively rare, and the diagnosis mainly depends on the history of inquiry, clinical manifestations and pathological examination. The treatment is mainly surgical resection, and future research should focus on finding a more effective treatment.

AlM: To investigate the effect of C. sinensis extracts on HBx (Hepatitis B virus X protein) induced cell proliferation and extracellular matrix (ECM) accumulation and the underlying mechanism in cultured human mesangial cells (HMCs). METHODS: Human mesangial cells were stable transfected with pCMV-HBx to establish an HBx over-expression model, and a control group was transfected with an empty vector. Cell proliferation was determined by MTT assay and DNA synthesis assay. Western blotting was used to measure the expression of PI3K/Akt signaling pathway-related proteins and extracellular matrix. RESULTS: HBx transfection induced cell proliferation, matrix accumulation. HBx-transfected mesangial cells had increased activity of the PI3K/Akt pathways, and treatment with C. sinensis suppressed this effect. CONCLUSlON: C.sinensis attenuates the HBx-induced human mesangial cell proliferation and matrix production in human mesangial cells via inhibition of the PI3K/Akt pathways.

Humans ; Leukemia, Lymphocytic, Chronic, B-Cell ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive

OBJECTIVETo explore the clinical effects of debridement and bone autografting combined with proximal femoral anatomical plate in treating benign tumor in proximal femur.

METHODSFrom January 2010 to October 2014, 30 patients with benign tumor in proximal femur were treated with debridement, autogenic ilium, autogenic ilium and allogeneic bone implant, and anatomic plate fixation. Among them, there were 13 males and 17 females aged from 12 to 68 years old with an average of 42 years old. The courses ranged from 1 month to 2 years with an average of 9 months. MSTS scoring were observed and compared before and after operation, and also applied to evaluate lower-extremity function. X-ray was examined to evaluate healing of focus. Postoperative complications were observed.

RESULTSAll patients were followed up from 12 to 48 months with an average of 29 months. MSTS score at the final following-up (27.06±2.59) was higher than preoperative (16.44±1.35), and there was significant difference(<0.05). X-ray at the final following-up showed bone graft fusion, pathological fracture were recover consciously, internal fixation was well, no loosening, deformation and displacement occurred. One case occurred incision fat liquefaction and 1 patient with giant cell tumor of bone relapsed at 13 months after operation.

CONCLUSIONSDebridement and bone autografting combined with proximal femoral anatomical plate is an effective method in treating benign tumor in proximal femur. It could control tumor, relieve pain, promote function and prevent occurrence of pathologic fractures.