Objective To establish allergic rhinitis(AR) model in rats to observe the changes in IL-5,IL-12 and IL-18 in serum of AR rats,and explore the influence of glucocorticoid on IL-5,IL-12 and IL-18 expression,and to elucidate further the pathogenesis of AR and the mechanism of glucocorticoid.Methods Thirty-six rats were divided into three groups:normal control(NC) group,AR group and dexamethasone intervention(Dex) group(12 each).Animals in AR and Dex group were sensitized with egg albumin,and in NC group were treated with normal saline as control.After sensitization and reproduction of AR model,rats in AR group received no treatment,while those in Dex group were treated with dexamethasone(5mg/kg,once a day) for 5 days.The changes in behavior was examined,and pathology of nasal mucosa were observed with HE staining,and the expressions of IL-5,IL-12 and IL-18 in serum were determined with ELISA.Results Compared with NC group,the frequency of sneesing and nose-scratching,and the expressions of IL-5 and IL-18 were increased obviously(P

Objective To explore the clinical effect of SaO2and heart rate(HR)for the diagnosis of acute mountain sickness (AMS).Methods A total of 1 062 male soldiers on garrison duty in rapidly entering to high altitude at 3 700-5 400 m from May 2013 to August 2015 were included as the research subjects.The demography data were collected and the AMS symptoms investiga-tion was performed.SaO2and HR were detected and the relationship between SaO2and HR at different altitudes with AMS symp-tom score was analyzed.Results The cut-off value of SaO2for diagnosing AMS in rapidly entering to high altitude at 3 700-<4 300 m was 84.5%(AUC=0.781)with the screening sensitivity of 78.31% and specificity of 72.02%;which of HR for diag-nosing AMS was 89.5 times/min(AUC=0.640)with the screening sensitivity of 71.27% and specificity of 54.63%.When SaO2 was serially connected with HR,its sensitivity was 58.87% and the specificity was 89.23%,while the parallel connection yielded a sensitivity of 90.70% and a specificity of 37.43%.In rapidly entering the altitude at above 5000 m,the cut-off value of SaO2for di-agnosing AMS was 80.5%(AUC=0.825)with the screening sensitivity of 84.62% and specificity of 68.85%;which of HR for di-agnosing AMS was 93.5 times/min(AUC= 0.718)with the screening sensitivity of 53.00% and specificity of 85.25%;when SaO2was serially connected with HR,its sensitivity for diagnosing AMS was 47.01% and specificity was 93.44%,while the paral-lel connection yielded a sensitivity of 90.60% and a specificity of 60.66%.Conclusion SaO2combined with HR can serve as an ob-jective index for the on-site diagnosis of AMS and is convenient for the AMS preliminary screening of large populations.

This experiment aimed to investigate the effect of adrenergic system in the subnucleus commissuriu of nucleus solitrius tractus (CNTS) on renal nerve discharges. Norepinephrine (NE) was microinjected into the CNTS of rabbits and mean arterial blood pressure (MAP) and renal nerve discharges (FRND) were synchronously recorded. The results indicated that (1) microinjection of norepinephine into the CNTS of rabbit could significantly attenuate the frequency of renal nerve discharge, and at the same time decrease markedly the mean arterial pressure. (2) Microinjection of 0.3 nmol yohimbin into CNTS had no significant influence on FRND and MAP, but could attenuate and even reverse the effects of NE on FRND and MAP. These results suggest that microinjection of NE into CNTS may activate the alpha-adrenorecptor located in CNTS and secondarily produce a depressor effect by attenuating the activity of periphenal sympathetic nervous system.
Blood Pressure/drug effects ; Depression, Chemical ; Kidney/*innervation ; Microinjections ; Norepinephrine/*pharmacology ; Solitary Nucleus/*physiology ; Sympathetic Nervous System/drug effects ; Sympathetic Nervous System/*physiopathology ; Vasomotor System/physiopathology

OBJECTIVE: To observe expression and distribution of histamine H4 receptor in nasal mucosa in normal people and allergic rhinitis patients,and understand role of histamine H4 receptor in allergic rhinitis. METHOD: Select normal people and allergic rhinitis patients each 10, take the nasal mucosa, detect expression and distribution of histamine H4 receptor at proteins and transcription level respectively by immunohistochemical method and RT-PCR, and compared. RESULT: Histamine H4 receptor at proteins and transcription level were found in normal nasal mucosa (25 509 +/- 6 441, 0.42 +/- 0.08), increased significantly in nasal mucosa of allergic rhinitis patients (49 676 +/- 8 541, 0.69 +/- 0.11, P < 0.05), which in structural cells and immune cells. CONCLUSION Histamine H4 receptors exist in normal nasal mucosa, its express significantly enhance, flew histamine H4 receptor may be mediated histamine in pathogenesis of allergic rhinitis ,who is one of the ligands of histamine.
Case-Control Studies ; Humans ; Nasal Mucosa ; immunology ; metabolism ; pathology ; Receptors, G-Protein-Coupled ; metabolism ; Receptors, Histamine ; metabolism ; Receptors, Histamine H4 ; Rhinitis, Allergic, Perennial ; immunology ; metabolism ; pathology

Summary: This experiment aimed to investigate the effect of adrenergic system in the subnucleus commissuriu of nucleus solitrius tractus (CNTS) on renal nerve discharges. Norepinephrine (NE) was microinjected into the CNTS of rabbits and mean arterial blood pressure (MAP) and renal nerve discharges (FRND) were synchronously recorded. The results indicated that (1) microinjection of norepinephine into the CNTS of rabbit could significantly attenuate the frequency of renal nerve discharge, and at the same time decrease markedly the mean arterial pressure. (2) Microinjection of 0.3 nmol yohimbin into CNTS had no significant influence on FRND and MAP, but could attenuate and even reverse the effects of NE on FRND and MAP. These results suggest that microinjection of NE into CNTS may activate the alpha-adrenorecptor located in CNTS and secondarily produce a depressor effect by attenuating the activity of peripheral sympathetic nervous system.