Utilizing the ~(125)I-Calmodulin-overlay technique, we have detected calmodulin-binding proteins (CaMBPs) in crude extracts of lymphocytes. The results indicated that appendix (B cell), mesenteric lymph node (T, B cell) and spleen (T, B cell) from rabbit contained 19, 20 and 16 species of CaMBPs respectively. Ten CaMBPs with molecular weight 80, 78, 70, 63,50, 41, 36, 22, 17 and 13.5 K D were present in all lymphocytes detected. Lymphocytes were rich in two kind of CaMBPs with molecular weight 17 and 13.5 K D which bound calmodulin in partial calcium-dependent manner.

Objective To establish a coupled model combining the rat brain nuclei microelectrode recordings and the behavioristics for rehabilitation experiment. Methods The modified indwelling tube connection fixed device was put inside the rats' back, and the microprobes were implanted into related neural nucleus. A signal connection was made between self-administration system and electrophysiological data acquisition system. The rat was addicted after training by self-administration system. The related cerebral nucleus electrophysiological sig-nals were recorded in different states of addiction. Results and Conclusion The modified indwelling tube connection fixed device has a bet-ter quality for reducing the phenomenon of leak. The signal was well in the combination of two different systems. The signals for the rat's ac-tion and neural electrical were recorded in the same time.

Objective To compare the clinical effect of first-line chemotherapy containing oxaliplatin on left and right colon cancer.Methods A total of 120 patients with colon cancer admitted to Second Affiliated Hospital of Shanghai University from January 2020 to January 2024 were selected and divided into left colon cancer group(60 cases)and right colon cancer group(60 cases)according to tumor location.Both groups were treated with first-line chemotherapy containing oxaliplatin for 2 cycles.Disease control rate,serum tumor markers,serum inflammatory factors,cancer-causing symptom scores,quality of life and incidence of adverse reactions were compared between two groups.Results After treatment,disease control rate in left colon cancer group was significantly higher than that in right colon cancer group(x2=22.224,P＜0.00 1).The levels of serum carcinoembryonic antigen,carbohydrate antigen 125,carbohydrate antigen 153,C-reactive protein and interleukin-6 of left colon cancer group were lower than those of right colon cancer group(P＜0.05).The scores of cancer-related pain and fatigue of left colon cancer group were lower than those of right colon cancer group(P＜0.05).The quality of life in left colon cancer group was better than that in right colon cancer group(P＜0.05).Conclusion First-line chemotherapy containing oxaliplatin has better short-term efficacy in patients with left colon cancer,with better control of tumor progression,and can improre clinical symptoms and quality of life.

Objective To explore the improvements of high-fat intake on lung injury induced by Paragonimus proliferus infection in rats, and to preliminarily explore the mechanisms underlying the role of cytochrome P450 4A1 (CYP 4A1) in the improve ments. Methods SD rats were randomly assigned into three groups, including the normal control group (n = 10), the infection and normal diet group (n = 12) and the infection and high-fat diet group (n = 12). Rats in the normal control group were fed with normal diet and without any other treatments, and animals in the infection and normal diet group were subcutaneously injected with 8 excysted metacercariae of P. proliferus via the abdominal wall, followed by feeding with normal diet, while rats in the infection and high-fat diet group were subcutaneously injected with 8 excysted metacercariae of P. proliferus via the abdominal wall, followed by feeding with high-fat diet. All rats were sacrificed 28 weeks post-infection, and serum samples and lung specimens were collected. Following hematoxylin-eosin (HE) staining of rat lung specimens, the rat lung injury was observed under an optical microscope, and alveolitis was evaluated using semi-quantitative scoring. Serum interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) levels were measured using enzyme-linked immunosorbent assay (ELISA), and the cytochrome P450 4A1 (CYP 4A1) expression was quantified in rat lung specimens at transcriptional and translational levels using quantitative real-time PCR (qPCR) and Western blotting assays. Results Alveolar wall thickening, edema and inflammatory cell infiltration were alleviated 28 weeks post-infection with P. proliferus in rats in the infection and high-fat diet group relative to the infection and normal diet group, and no alveolar consolidation was seen in the infection and high-fat diet group. The semi-quantitative score of alveolitis was significantly higher in the infection and normal diet group [(2.200 ± 0.289) points] than in the normal control group [(0.300 ± 0.083) points] and the infection and high-fat diet group [(1.300 ± 0.475) points] (both P values < 0.05), and higher serum IL-1β [(151.586 ± 20.492)] pg/mL and TNF-α levels [(180.207 ± 23.379) pg/mL] were detected in the infection and normal diet group than in the normal control group [IL-1β: (103.226 ± 3.366) pg/mL; TNF-α: (144.807 ± 1.348) pg/mL] and the infection and high-fat diet group [IL-1β: (110.131 ± 12.946) pg/mL; TNF-α: (131.764 ± 27.831) pg/mL] (all P values < 0.05). In addition, lower CYP 4A1 mRNA (3.00 ± 0.81) and protein expression (0.40 ± 0.02) was quantified in lung specimens in the infection and normal diet group than in the normal control group [(5.03 ± 2.05) and (0.84 ± 0.14)] and the infection and high-fat diet group [(11.19 ± 3.51) and (0.68 ± 0.18)] (all P values < 0.05). Conclusion High-fat intake may alleviate lung injuries caused by P. proliferus infection in rats through up-regulating CYP 4A1 expression in lung tissues at both translational and transcriptional levels.