Objective To discuss the clinical manifestation of top of the basilar artery syndrome (TOBS) and its features of magnetic resonance imaging (MRI)/cerebral digital subtraction angiography (DSA). Method The clinical data of 30 cases with TOBS admitted during past four years were analyzed. Results The clinical manifestation of TOBS comprised the sudden vertigo and unconsciousness with the ophthalmoplegia and the abnormality of the pupils,as well as the paralysis,partial blindness or cortical blindness and ataxia and memory impairment. MRI showed the local infarction in thalami, cerebellum, midbrain, lobus occipitals , pons, temporal inner surface and splenium of corpus callosum. The features of DSA are occlusion and stenosis in basilar artery and vertebral artery. Conclusions The diagnose of TOBS depends mainly on the clinical manifestation and MRI. The DSA application in TOBS can offer the possible location of vascular lesion, and instruct treatment and prevention for TOBS.

Objective To study the surgical treatment of nodular goiter.Methods The surgical treatment effects of 248 cases with nodular goiter were reviewed.Results 123 paitients were cut off the merely nodle,83 patients suffered one side subtotal lobectomy,32 patients suffered doublr subtotal lobectomy, 10 patients suffered one side merely nodle plus oppside subtotal lobectomy, and 12 patients received operations twice,including 1 case (temporarily) of permanent injury of parathyroid, 1 case(temporarily) of injury of recurrent laryngeal never,3 cases of postoperative hemorrhage.All patients were followed up from 1 to 8 years,and no relapse was noted after operation.Conclusion One side thyroid gland nodle or nodles should cut off the merely nodle and it is unneceeary to deal with the blood vessel of the thyroid up and down,one side or double side thyroid gland nodles tightly should operated subtotal lobectomy, to ligate the blood vessel of the thyroid down, not to dispose the recuyyent laryngeal nerve,reserving the left over gland in cyst,and to prevent hypothyroidism and recurrent laryngeal nerve.

Objective To observe the protective effects of quercetin on esophageal mucosa in chronic mixed reflux esophagitis (RE) rats and the effect of quercetin on nuclear factor (NF)-κB/interleukin (IL)-6 signaling pathway.Methods Mixed RE model was successfully induced by cardia ligation and esophagoduodenostomy.48 healthy male Sprague-Dawley rats were equally divided into the following 6 groups using random number table method:normal control group,sham-operation group,model control group,omeprazole group,low-dose quercetin group,and high-dose quercetin group.The 6 groups were treated with peritoneal injection of 2 ml normal saline (normal control,sham-operation,model control groups),20 mg/kg omeprazole,100 mg/kg quercetin (low-dose) and 200 mg/kg quercetin (high-dose) once daily,respectively.The rats were sacrificed after 6 weeks of intervention.The microscopic pathological changes of esophageal mucosa were scored.NF-κB p65 and IL-6 protein levels in esophageal mucosa and serum were assessed using immunohistochemistry and enzyme-linked immunosorbent assay,respectively.Results In normal control group,shamoperation group,model control group,omeprazole group,low-dose quercetin group and high-dose quercetin group,the pathological scores of esophageal mucosa were 0.250 ± 0.463,0.250 ± 0.463,2.625 ± 0.518,1.500 ±0.535,1.250 ±0.463,and 1.375 ±0.518; the NF-κB p65 protein scores in esophageal mucosa were 0.500±0.535,0.625 ±0.518,3.500 ±0.535,1.875 ±0.649,1.750 ±0.707,and 2.000 ±0.535; the IL-6 protein scores in esophageal mucosa were 1.125 ± 0.641,1.125 ± 0.835,5.375 ± 0.518,2.375 ± 0.518,2.000 ±0.535,and 2.250 ±0.463; the serum NF-κB p65 protein levels were (68.618 ± 18.500),(77.824 ± 22.228),(184.882 ± 49.165),(106.693 ± 45.312),(76.215 ± 16.588),and (108.207 ± 42.107) pg/ml; the serum IL-6 protein levels were (24.826 ±4.008),(23.599 ±4.351),(51.378 ± 9.697),(32.370 ± 11.657),(23.085 ± 4.660),and (26.243 ± 4.955) pg/ml.In terms of the 5 indicators,there were no statistically significant differences between the normal control group and the sham-operation group (P =1.000,P =0.642,P =1.000,P =0.518,P =0.673) ; the results in the normal control,shamoperation,omeprazole,low-dose quercetin,and high-dose quercetin groups were significantly different from those in the model control group (P < 0.001,P < 0.001,P < 0.001,P =0.002,P =0.001 ; P < 0.001,P < 0.001,P<0.001,P=0.004,P=0.002; P=0.001,P<0.001,P<0.001,P=0.025,P=0.023; all P <0.001 ; P <0.001,P <0.001,P <0.001,P =0.023,P <0.001) ; there were no statistically significant differences between low-dose quercetin group and omeprazole group,nor between high-dose quercetin group and omeprazole group (P=0.334,P=0.717,P=0.176,P=0.121,P =0.074; P =0.642,P=0.678,P=0.619,P =0.949,P =0.225); there were no statistically significant differences between low-dose quercetin group and high-dose quercetin group (P =0.619,P =0.438,P =0.334,P =0.086,P =0.243).The microscopic pathological score of esophageal mucosa was positively correlated with NF-κB p65 and IL-6 protein scores in esophageal mucosa (r =0.803,P < 0.001 ; r =0.758,P < 0.001),also positively correlated with serum NF-κB p65 and IL-6 protein levels (r=0.486,P=0.004; r=0.544,P=0.001).Conclusions The expression levels of NF-κB p65 and IL-6 protein in esophageal mucosa and serum increase with the severity of esophageal mucosal injury.Quercetin can reduce the severity of esophageal mucosal injury in RE,possibly through down-regulating NF-κB and IL-6 expression and mitigatng esophageal inflammatory status.

Objective To compare and analyze the influencing factors of the distribution and drug resistance of blood culture positive pathogens in neonatal intensive care unit (NICU) at different altitude areas.Methods The distribution of blood culture positive pathogens and clinical susceptibility of children in NICU of two different altitude hospitals in 2015 were retrospectively analyzed.Results In 2015,children in NICU in upper elevation district hospital mainly infected with 19 strains(18.4％) of epidermis staphylococcus,18 strains(17.5 ％) of Escherichia coli,14 strains(13.6 ％) of Klebsiella pneumoniae,14 strains(13.6 ％) of Hemolysis staphylococcus,12 strains(11.7 ％) of Stenotrophomonas maltophilia;Children in NICU at low altitude hospital mainly infected with 31 strains(19.7％) of epidermis staphylococcus,27 strains(17.2％) of Achromobacter xylosoxidans,18 strains(11.5％) of Hemolysis staphylococcus,14 strains(8.9 ％) of Klebsiella pneumoniae,14 strains (8.9 ％) of Acinetobacter baumannii.The detection rate of Gram-negative bacilli in high altitude hospital was higher,and the detection rate of Gram positive cocci in low altitude hospital was higher.In high-altitude district hospital,the detection rate of methicillin-resistant coagulase negative staphylococci (MRCNS) extended-spectrum β-lactamase (ESBLs),and multidrug-resistant Acinetobacter baumannii(MDRAB) were than low altitude hospital.Conclusion Escherichia coli and Stenotrophomonas maltophilia detection rate and common antibiotics sensitive rate are relatively high at upper elevation areas;Detection rate of coagulase negative Staphylococcus aureus and common antibiotics resistance rate are high in low altitude.Different altitudes environmental factors may play an important role in pathogens distribution and drug resistance from NICU blood culture.

ONYX-015 and H101 are E1B 55-kDa protein-deficient replicating C group adenoviruses that are currently in clinical trials as antitumor agents. However, their application in cancer gene therapy is limited by the native tropism of C group adenoviruses. This is in part due to low expression of the C group adenovirus receptor (coxsackievirus-adenovirus receptor, CAR) on malignant tumors. An H101-based chimeric virus vector containing sequences encoding the Ad35 fiber domain instead of the Ad5 fiber (H101-F35) was constructed. This modification allowed infection of tumor cells through CD46, a membrane protein over-expressed on tumors. The CAR and CD46 RNA expression was evaluated by RT-PCR method. H101-F35 conferred a stronger cytocidal effect than H101 and ONYX-015 in tumor cell lines that lacked CAR expression (MDA-MB-435 and MCF-7), while the cytocidal effect of H101-35, H101 and ONYX-015 was similar in high-level CAR expressing cancer cell lines (A549, NCI-H446, Hep3B, LNCaP, ZR-75-30 and Bcap-37). In an MDA-MB-435 xenograft mouse tumor model, tumor growth in mice receiving H101-F35 was significantly inhibited compared with mice injected with H101. These results suggest that the chimeric oncolytic adenovirus H101-F35 vector might be a useful candidate for gene therapy of cancer.

Objective To observe the clinical efficacy of autologous hair follicle stem cell trans-plantation for hair restoration in patients with alopecia.Methods Ten patients with alopecia,inclu-ding five with androgenic alopecia and five with seborrheic alopecia,were selected from November 2022 to May 2023.They were treated with autologous hair follicle stem cell transplantation.Data be-fore treatment were used as the control group,and data after treatment were used as the study group.Follow-up was conducted for 3 to 8 months.The FPF scoring system was designed to collect data and evaluate the treatment effect.Results Nine patients had an improvement score of ≥7 points,and one patient had an improvement score of 2 points,with a treatment excellence rate of 90％(9/10).Compared with the total score of(2.81±0.92)points in the control group,the total score of the study group was(9.00±2.21)points,and the difference was statistically significant(P＜0.001).Conclusion Autologous hair follicle stem cell transplantation for hair restoration is safe and effec-tive,and has a broad range of indications.

Objective To observe the clinical efficacy of autologous hair follicle stem cell trans-plantation for hair restoration in patients with alopecia.Methods Ten patients with alopecia,inclu-ding five with androgenic alopecia and five with seborrheic alopecia,were selected from November 2022 to May 2023.They were treated with autologous hair follicle stem cell transplantation.Data be-fore treatment were used as the control group,and data after treatment were used as the study group.Follow-up was conducted for 3 to 8 months.The FPF scoring system was designed to collect data and evaluate the treatment effect.Results Nine patients had an improvement score of ≥7 points,and one patient had an improvement score of 2 points,with a treatment excellence rate of 90％(9/10).Compared with the total score of(2.81±0.92)points in the control group,the total score of the study group was(9.00±2.21)points,and the difference was statistically significant(P＜0.001).Conclusion Autologous hair follicle stem cell transplantation for hair restoration is safe and effec-tive,and has a broad range of indications.

Translationally controlled tumor proteins (TCTP) and SNF1- related protein kinase (SnRK1) are conserved and widely present in eukaryotic cells. TCTP regulates cell division, plant growth and development, and mediates plant resistance against pathogen infection. SnRK1 participates in a range of physiological processes including sugar metabolism and resistance to abiotic and biotic stresses. Previous work in our laboratory demonstrated that wheat TCTP can respond to Puccinia triticina infection and induce host defense responses. In order to further investigate the mechanism of TaTCTP in wheat resistance to Puccinia triticina infection, we used TAP (tandem affinity purification) and mass spectrometry to screen the potential interactants of TaTCTP. A SNF1- related protein kinase (SnRK1) was identified as a potential interacting protein of TaTCTP. The results of yeast two-hybrid assay showed that TCTP could interact with SnRK1 in yeast, and the yeast carrying TCTP and SnRK1 could grow on SD/-Leu/-Trp/-His/-Ade (SD/-LWHA) medium. The fluorescence signal of the interaction between TCTP and SnRK1 was found to be distributed in the cytoplasm in the Bi-fluorescense complementation experiment. Co-IP experiments further showed that TCTP and SnRK1 could interact in plant cells. This study lays an important foundation for further studying the mechanism of TaTCTP in the interaction between wheat and Puccinia triticina, and it play a great influence on further improving the molecular mechanism of wheat resistant to Puccinia triticina.
Basidiomycota ; Humans ; Neoplasms ; Protein Biosynthesis ; Protein-Serine-Threonine Kinases ; Triticum