Objective To isolate and identify leukemia stem cells from acute myeloid leukemia patients for further research. Methods By Ficoll density gradient centrifugation, mononuclear cells were firstly separated from bone marrow of patients. According to specific surface markers, CD+34 CD+123 of leukemic stem cells were sorted by flow cytometer. Their ability of self-renewal and differentiation were evaluated by colony formation and cobblestone forming ability. At the same time, the purity and cell morphology of CD+34 CD+123 cells was analysed. Results Comparared with total mononuclear cells, the proportion of the CD+34 CD+123 cells after sorting was 10.7 %, and these cells showed the ability of colony forming and cobblestone forming, and the purity proportion of CD+34 CD+123 cells was 62.1%. Conclusion The leukemia stem cells were isolated successfully and could be used in further study.

Objective To evaluate the maneuvre of curettage and aspiration(LTCA) in laparoscopic hepatectomy. MethodsWe used Peng′s multifunctional operative dissector(PMOD) to perform laparoscopic liver transection by maneuvre of curettage and aspiration in 20 cases undergoing laparoscopic hepatectomy. Results Procedures were all successful. The recovery was uneventful without any complications. Mean operative time was 105 minutes, the average bleeding volume was 420 ml, the largest excised sample size was 10 cm?9 cm?7 cm. All patients were discharged within one week. ConclusionsThe new technique-LTCA can be used in laparoscopic hepatectomy, it has the advantages of clear anatomy, good exposure of canal construction, rapid liver transection, satisfactory hemostasis and clear operative field.

Objective To explore the effect of ectopic splitting combined with portal vein ligation on rat liver regeneration.Methods Sixty Sprague-Dawley rats were randomly divided into four groups (n =15):portal vein ligation group (PVL group),associating liver partition with PVL group (ALPP group),associating spleen partition combined with PVL group (ASPP group) and associating kidney partition combined with PVL group (AKPP group).Hepatic regeneration rate (HRR),ALT,AST and hepatocyte proliferating cell nuclear antigen (PCNA) were detected on the 2nd,4th and 7th day after operation.Results On the 4th and 7th day,in ALPP group,ASPP group and AKPP group,there was an obvious hyperplasia (F =3.750,P ＜ 0.05;F =16.398,P ＜ 0.01) in the right middle lobe of the liver and higher PCNA (F=6.860,P＜0.01;F=3.810,P＜0.05) in the liver tissue than in PVL group.And ALPP group had significantly higher ALT and AST level on the 2nd and 4th day (ALT:F =8.812,F =21.929;AST:F =36.393,F =6.468;P ＜ 0.01).Conclusions Ectopic splitting combined with PVL can significantly promote rat's liver regeneration,possibly by inflammatory response,stress response and high expression of certain cytokines.

Antineoplastic Agents ; chemistry ; pharmacology ; Carcinoma, Hepatocellular ; drug therapy ; immunology ; pathology ; Cytokines ; immunology ; Drug Screening Assays, Antitumor ; Glypicans ; antagonists & inhibitors ; immunology ; Humans ; Ligands ; Liver Neoplasms ; drug therapy ; immunology ; pathology ; T-Lymphocytes ; drug effects ; immunology