Objective To investigate the protective effect of heme oxygenase-1 for xeno-heart transplantation and the possible mechanism.Methods Cobalt protoporphyrin (CoPP), HO-1 inducer, and zinc protoporphyrin (ZnPP), HO-1 inhibitor, were used to intervene donors and recipients on the model of NIH-Wistar cardiac transplants respectively and simultaneously some of recipients were treated with immunesuppressor cyclosporine A (CsA), and control group and CsA treated group were set up respectively. The expression of HO-1 protein, HO-1 mRNA, caspase-3 and STAT-3 in transplanted heart tissue was detected by immunohistochemistry, RT-PCR and Western Blot. At the same time, HO-1 enzymatic activity was examined in heart tissue, and the cardiac cell apoptosis was examined by means of TUNEL. The differences among various factors were compared.Results The survival time of cardiac transplants in CoPP group was longer than that in control and ZnPP groups (P

Objective To explore the mechanism of delayed xenograft rejection(DXR) by dynamic observation of histological and immunohistologic changes in mouse-to-rat cardiac xenografts.Methods A model of mouse-to-rat cardiac heterotopic xenotransplantation in neck was established by cuff technique.NIH mouse hearts not transplanted served as controls(n=4).Some xenograft recipients were killed and cardiac xenografts harvested at end of 3,8,16,24 h(n=4 for each time point) after transplantation.The cardiac hearts(n=16) of some xenograft recipients were not harvested until rejection time to determine their survival time.All heart samples were examined by HE and immunohistochemistry for semi-quantitative determination of antibodies including C3,IgM,IgG,E-selectin and macrophage marker——CD68.Results During the period after transplantation,the degree of rejection of xenografts became more and more serious till ultimate rejection.The mean survival time of the xenografts was(49.3?16.2) h.Immunohistochemical examination showed C3 were not detected in the xenografts at any time during the course of rejection;From 3 h after transplantation,obvious deposition of IgM was found in the grafts and IgG deposition got abundant;E-selectin expression was found as early as 3 h after transplantation and increased gradually;There was progressive infiltration by macrophages in the grafts.Conclusion Mouse-to-rat cardiac xenotransplantation can serve as an animal model of DXR.Endothelial cells activation,IgM and IgG,macrophage infiltration involve in DXR development except C3.

Objective To discuss the effects of 6Emodel on stress distress in patients with colorectal cancer after permanent intestinal stoma and to provide reference for the implementation of psychological intervention. Methods A total of 168 patients with permanent intestinal stoma were divided into control group(85 cases)and intervention group(83 cases).In the control group,routine nursing was adopted,while in intervention group,nursing based 6Emodel (including experience,environment,event,engaging,effect,expand)was adopted.The time of intervention was 1 year,and the psychological distress scale for cancer patients was tested.Results After interven-tion,the psychological distress of the intervention group was (39.62 ±14.62)points,which was lower than (46.21 ± 13.68)points of the control group,the difference was statistically significant between the two groups(t =3.057,P =0.002).Conclusion The 6Emodel can reduce the level of psychological distress of colorectal cancer after per-manent intestinal stoma,and has positive effects on the early distress following stoma.The intervention can be one of the effective ways to implement psychological intervention for patients with stoma.

Objective To discuss the effects of simulation -based team training on early adaptation and stress in patients with permanent intestinal stoma.Methods 164 patients with permanent intestinal stoma were divided into control group(82 cases)and intervention group(82 cases).In control group,routine peri -operational health education was adopted,while in intervention group,health education based simulation -based team training was adopted. Then,the intervention effects of ostomy adjustment inventory and stress distress were evaluated.Results After the intervention,there was significant difference between two groups at the level of ostomy adjustment inventory and stress distress(all P <0.05).Conclusion The simulation -based team training could increase the level of ostomy adjust-ment inventory,but reduce the level of stress distress.

Objective To investigate the expression patterns and significance of aquaporin 5 (AQP‐5) and multidrug‐resistance associated genes in human colon cancer with different differentiation degree and their correlation .Methods The expression of aqua‐porin 5 and resistance genes P‐gp ,GST‐π,TopoⅡ ,and TS in human 45 cases colon cancer tissues with different differentiation de‐gree and 36 cases of adjacent mucosa tissues as well as 58 cases of normal colonic epithelium were detected by quantitative RT‐PCR ,Western blot and immunohistochemistry .Results Immunohistochemistry results showed that AQP‐5 distributed mainly in the cell membrane and the cytoplasm .Fluorescence quantitative RT‐PCR and Western blot showed that AQP‐5 expression could not be detected in adjacent mucosa tissues and normal colonic epithelium tissues .The AQP‐5 expression level was higher in colon cancer tissues compared with adjacent mucosa tissues and normal colonic epithelium tissues (P<0 .05) ,and the expression intensity was correlated with the differentiation degree of colon cancer tissues (P<0 .05) .The results of immunohistochemistry indicated that P‐gp distributed mainly in the cell membrane and the cytoplasm ,GST‐πmainly distributed in the nuclei and cytoplasm ,Topo Ⅱ main‐ly distributed in the nucleus ,and TS distributed mainly in the cytoplasm .Fluorescence quantitative RT‐PCR and Western blot re‐sults showed that the expression levels of all resistance genes detected were higher in colon cancer tissues compared with adjacent mucosa tissues and normal colonic epithelium tissues (P<0 .05) .Furthermore ,P‐gp ,GST‐π,and Topo Ⅱ expression were negative‐ly correlated with the differentiation degree of colon cancer tissues ,the more poor differentiation level of tissue ,the higher expres‐sion level of P‐gp ,GST‐π ,Topo Ⅱ .However ,the expression level of TS did not change significantly in different differentiation de‐gree colon cancer tissues (P>0 .05) .Positive correlation was found between the expression of AQP‐5 and P‐gp ,GST‐π,Topo Ⅱ(P<0 .05) .Negative correlation was found between the expression of AQP‐5 and TS (P>0 .05) .Conclusion The AQP‐5 and re‐sistance gene expression were increased in colon cancer tissues .The AQP‐5 expression level was higher in colon cancer compared with adjacent control or normal tissues ,which may promote the transfer and progress of colon cancer .

10.3969/j.issn.1007-3969.2013.04.007

Purpose: Effective predictors of the response to neoadjuvant chemotherapy (NAC) are still insufficient. This study aimed to investigate the predictive value of serum lipid profiles for the response to NAC in breast cancer patients. Methods: A total of 533 breast cancer patients who had received NAC were retrospectively studied. The pretreatment of serum lipids, including total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and lipoprotein-α, and clinicopathological characteristics were collected to assess their predictive roles. Results: Breast cancer patients had significantly lower TC, TG, HDL-C, and LDL-C levels than normal individuals. Among these indicators, TG and LDL-C levels and HDL-C level increased and decreased significantly after NAC, respectively. In estrogen receptor (ER)-positive patients, increased LDL-C level was associated with better outcomes. Moreover, the receiver operating characteristic curve analyses suggested that TG and HDL-C levels at diagnosis can be used as predictors of the response to NAC only in the ER-positive subgroup.According to univariate analyses, patients with low TG level (< 1.155 mmol/L) or high HDL-C level (≥ 1.305 mmol/L) in the ER-positive subgroup had more favorable clinical responses than the other patients in the subgroup. Furthermore, according to multivariate analyses, a high HDL-C level (≥ 1.305 mmol/L, p = 0.007) was an independent predictor of NAC efficacy. Conclusion High HDL-C level (≥ 1.305 mmol/L) before NAC and increased LDL-C level after NAC were associated with the better treatment response in ER-positive breast cancer patients.These results are potentially considered beneficial in establishing treatment decisions.