Objective To study the chemical constituents from Onychium japonicum.Methods Che-mical constituents were isolated by repeated column chromatography and preparative HPLC,and their structures were elucidated on the basis of spectroscopic method.Results Ten compounds were identifed as:4,3',4'-trihydroxy-2,6-dimethoxychalcone(1),chrysoeriol(2),luteolin(3),butin(4),protocatechuic acid(5),3,4-dihydroxy-acetophenone(6),caffeic acid(7),vanillic acid(8),2,4-dihydroxybenzaldehyde(9),syringic acid(10),and ?-sitosterol(11).Conclusion Compound 1 is a new product named japonicone D,compounds 2-10 are isolated from the plants of Onychium Kaulf for the first time.

OBJECTIVETo study the anti-tumor metastatic constituents from Ardisia Crenata.

METHODChemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.

RESULTNine compounds(1-9) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: 5-hydroxymethyl-2-furalclehyde(1), ethyl-beta-D-fructopyranoside(2), syringic acid(3), n-butyl-beta-D-fructofuranoside(4), n-butyl-alpha-D-fructofuranoside(5), methyl-alpha-D-fructofuranoside(6), (+)-bergenin(7), ardisiacrispins B(8), asperuloside acid(9). The isolated compounds(1-9) showed positive anti-tumor metastatic activities, and compounds 1, 5, and 8 showed significant anti-tumor metastatic activities. At the concentration of 0.8 mg x L(-1), compound 5 revealed the value of metastatic inhibition ratio on MDA-MB-231 was 93.8%.

CONCLUSIONCompounds 2-6 and 9 were isolated from this plant for the first time. compounds 1, 5 and 8 showed significant anti-tumor metastatic activities.

Antineoplastic Agents ; pharmacology ; therapeutic use ; Ardisia ; chemistry ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Neoplasm Metastasis ; drug therapy

OBJECTIVETo study the anti-tumor metastatic constituents from Lindera glauca.

METHODConstituent isolation and purification was carried by repeated column chromatography (silica gel, Toyopearl HW-40 and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.

RESULTTen compounds (1 - 10) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: Laurotetanine (1), N-methyllaurotetanine (2), reticuline (3), pallidine (4), N-trans-feruloyltyramine (5), N-cis-feruloyltyramine (6), atheroline (7), norisosocorydine (8), [9,9,9-(2) H3]-(1S*, 3S*, 4S*, 8S*)-p-menthane-3,8-diol (9), [9,9,9-(2) H3 ]-(1S*, 3R*, 4S*, 8S*)-p-Menthane-3,8-diol (10). Compounds 1, 2, 4, 5, 7 and 9 showed positive anti-tumor metastatic activities,and compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.

CONCLUSIONCompound 3 was isolated from this plant for the first time. Compounds 9 and 10 were isolated from Lindera genus for the first time. Compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.

Alkaloids ; chemistry ; isolation & purification ; Antineoplastic Agents, Phytogenic ; chemistry ; isolation & purification ; Aporphines ; chemistry ; isolation & purification ; Benzylisoquinolines ; chemistry ; isolation & purification ; Cell Line, Tumor ; Chromatography, High Pressure Liquid ; methods ; Humans ; Lindera ; chemistry ; Monoterpenes ; chemistry ; isolation & purification ; Neoplasm Metastasis ; prevention & control ; Plant Extracts ; chemistry ; isolation & purification

OBJECTIVETo study the immunosuppressive constituents from Tetraena mongolica.

METHODChemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The MTT assay was applied to evaluate the isolated compounds on the inhibition effect of lymphocyte transformation.

RESULTSix triterpenes were isolated and their structures were identified as follows: 3beta-hydroxy-11alpha, 12alpha:13beta,28-diepoxyoleanane(1), 3beta-(3, 4-dihydroxycinnamoyl)-erythrodi-ol(2), olean-28-al-3beta-yl-caffeate(3), erythrodiol (4), 12-oleanaen-3beta-caffeate(5), 3-O-(E) -coumaroylerythrodiol(6). Compound 24 exhibited the inhibition effects on lymphocyte transformation.

CONCLUSIONCompounds 1-6 were isolated from this plant for the first time, and compound 1 was a new nature product. Compound 2-4 showed significant immunosuppressive activity.

Animals ; Cells, Cultured ; Immunosuppressive Agents ; chemistry ; isolation & purification ; pharmacology ; Lymphocyte Activation ; drug effects ; Magnetic Resonance Spectroscopy ; Male ; Mice ; Mice, Inbred BALB C ; Triterpenes ; chemistry ; isolation & purification ; pharmacology ; Zygophyllaceae ; chemistry