1.The Extraction Method of Bacterial DNA from Activated Sludges
Wei ZHENG ; Chun-Shan QUAN ; Yong-Zhe PIAO ; Jun-Hua WANG ; Sheng-Di FAN ;
China Biotechnology 2006;0(07):-
Methods for studying the population diversity of microorganism in activated sludge usually require enrichment of bacterial genome.The efficient information on microbial species composition provided and shifted in diversity revealed are dependent on the effective DNA recovery technique.The method was based on washing by alkaline phosphate buffer and digestion with extended heating of the activated sludge suspension in the presence of lysozyme and freeze-thawing in high-salt-SDS buffer.The extraction was tested for four activated sludge differing in places and dates.The DNA fragment from all sludge was integrity.DNA yields ranged from 105 to 823 ?g/g sludge and were of sufficient purity for PCR-based 16S ribosomal DNA analysis and restriction digested.In general,all methods produced DNA pure were not enough for PCR amplification and libraries construction.As basis of experimental goals,the study provides an appropriate extraction method of microbial DNA in sludge.
2.Surveillance and analysis of Haemaphysalis longicornis with SFTS virus in Tumen river basin located in the frontiers of Russia, Korea and Northeast China
Ji-xu LI ; Shin-Hyeong CHO ; Wen PIAO ; Guang-jun JIN ; Zhen-hai SONG ; Sheng-huan QUAN ; Guang-ming PIAO
Chinese Journal of Disease Control & Prevention 2019;23(2):201-205,211
Objective To mastered the distribution characteristics of Haemaphysalis longicornis in the Tumen River basin along the border between Russia, Korea and Northeast China, and understand the status of Haemaphysalis longicornis carrying the virus of fever with thrombocytopenia syndrome (SFTS), then, isolate the virus and analyze its genetic characteristics. Method Ticks were collected from Hunchun, Tumen, Helong and Longjing cities in the Tumen River basin of Jilin Province from April to September, 2017. Haemaphysalis longicornis was selected and grouped. SFTS virus was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Virus isolation was carried out in Vero cells, besides, S,M,L gene segments were amplified . The homology of S, M and L gene segments was compared, phylogenetic tree was established, and their gene characteristics were analyzed. Result Haemaphysalis longicornis mainly distributed in Hunchun and Tumen City in the lower reaches of Tumen River. It was the dominant species in the two counties, reaching 71.85% and 87.62% respectively. A virus named YBHC-TICK2-2017/CHINA was isolated from Haemaphysalis longicornis collected in Hunchun. The sequences of S segment (1 746 bp), M segment (3 336 bp) and L segment (6 376 bp) of the virus were 98.00%-99.00%, homologous to those of SFTS virus isolates from China and Korea recorded in National Center for Biotechnology Information (NCBI) Gen Bank. Phylogenetic tree analysis showed that the S segment gene sequence of the virus strain was divided into a cluster with Jilin strain (KT890282) in China, and M segment and L segment gene sequence with Jiangsu strain (KR230781) in China. Conclusions Haemaphysalis longicornis are widely distributed in the lower reaches of the Tumen river. It was the first time that SFTS virus was isolated from Haemaphysalis longicornis in this area, suggesting that this area is important for SFTS prevention.
3.NaFeEDTA fortified soy sauce showed higher iron absorption rate in Chinese females.
Jun-Sheng HUO ; Xiao-Guang YANG ; Jian-Hua PIAO ; Jun-Quan GAO ; Hong MIAO ; Bo YU ; Cheng-Qian LU ; Jun-Shi CHEN
Biomedical and Environmental Sciences 2007;20(2):126-130
OBJECTIVENaFeEDTA was considered as a promising iron fortificant for controlling iron deficiency anemia. Soy sauce is a suitable food carrier for iron fortification and is a popular condiment in China. Iron absorption rates of NaFeEDTA and FeSO4 were observed and compared in adult female subjects.
METHODSThe stable isotope tracer method was used in Chinese females consuming a typical Chinese diet. Ten healthy young Chinese women were selected as subjects in the 15-day study. A plant-based diet was used based on the dietary pattern of adult women in the 1992 National Nutrition Survey. Six milligram of 54Fe in 54FeSO4 soy sauce and 3 mg 58Fe in Na58FeEDTA soy sauce were given to the same subjects in two days. Food samples and fecal samples were collected and analyzed.
RESULTSIron absorption rates of NaFeEDTA and FeSO4 were 10.51% +/- 2.83 and 4.73% +/- 2.15 respectively. The 58Fe (NaFeEDTA) absorption was significantly higher than that of 54Fe (FeSO4) (P < 0.01). The iron absorption rate from NaFeEDTA was 1.2 times higher than that from FeSO4 in Chinese adult women consuming a typical Chinese diet.
CONCLUSIONThe higher absorption rate of NaFeEDTA suggested that NaFeEDTA would be a better iron fortificant used in soy sauce for the controlling of iron deficiency anemia in China.
Adolescent ; Adult ; China ; Edetic Acid ; pharmacokinetics ; Female ; Ferric Compounds ; pharmacokinetics ; Ferrous Compounds ; pharmacokinetics ; Food, Fortified ; Humans ; Iron ; pharmacokinetics ; Soy Foods
4.Parkinson's Disease with Fatigue: Clinical Characteristics and Potential Mechanisms Relevant to α-Synuclein Oligomer.
Li Jun ZUO ; Shu Yang YU ; Fang WANG ; Yang HU ; Ying Shan PIAO ; Yang DU ; Teng Hong LIAN ; Rui Dan WANG ; Qiu Jin YU ; Ya Jie WANG ; Xiao Min WANG ; Piu CHAN ; Sheng Di CHEN ; Yongjun WANG ; Wei ZHANG
Journal of Clinical Neurology 2016;12(2):172-180
BACKGROUND AND PURPOSE: The aim of this study was to identify the clinical characteristics and potential mechanisms relevant to pathological proteins in Parkinson's disease (PD) patients who experience fatigue. METHODS: PD patients (n=102) were evaluated using a fatigue severity scale and scales for motor and nonmotor symptoms. The levels of three pathological proteins-α-synuclein oligomer, β-amyloid (Aβ)(1-42), and tau-were measured in 102 cerebrospinal fluid (CSF) samples from these PD patients. Linear regression analyses were performed between fatigue score and the CSF levels of the above-listed pathological proteins in PD patients. RESULTS: The frequency of fatigue in the PD patients was 62.75%. The fatigue group had worse motor symptoms and anxiety, depression, and autonomic dysfunction. The CSF level of α-synuclein oligomer was higher and that of Aβ1-42 was lower in the fatigue group than in the non-fatigue group. In multiple linear regression analyses, fatigue severity was significantly and positively correlated with the α-synuclein oligomer level in the CSF of PD patients, after adjusting for confounders. CONCLUSIONS: PD patients experience a high frequency of fatigue. PD patients with fatigue have worse motor and part nonmotor symptoms. Fatigue in PD patients is associated with an increased α-synuclein oligomer level in the CSF.
Anxiety
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Cerebrospinal Fluid
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Depression
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Fatigue*
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Humans
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Linear Models
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Parkinson Disease*
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Weights and Measures
5.Improved anti-tumor efficiency against prostate cancer by docetaxel-loaded PEG-PCL micelles.
Ming-ji JIN ; Sheng-jun PIAO ; Tie-xiong JIN ; Zhe-hu JIN ; Xue-zhe YIN ; Zhong-gao GAO
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(1):66-75
This study primarily focused on the systematic assessment of both in vitro and in vivo anti-tumor effects of docetaxel-loaded polyethylene glycol (PEG)2000-polycaprolactone (PCL)2600 micelles on hormone-refractory prostate cancer (HRPC). By using solvent evaporation method, PEG-PCL was chosen to prepare doxetaxel (DTX)-loaded mPEG-PCL micelles (DTX-PMs), with the purpose of eliminating side effects of the commercial formulation (Tween 80) and prolonging the blood circulation time. The prepared DTX-PMs had an average particle size of 25.19±2.36 nm, a zeta potential of 0.64±0.15 mV, a polydispersity index of 0.56±0.03, a drug loading of (8.72±1.05)%, and an encapsulation efficiency of (98.1±8.4)%. In vitro cytotoxicity studies indicated that DTX-PMs could effectively kill LNCap-C4-2B cells and show a dose- and time-dependent efficacy. The hemolysis test showed that DTX-PMs had less hemocytolysis than the commercial product of Duopafei®. A sustained in vitro release behavior and prolonged circulation time in blood vessels were observed in the DTX-PMs. Furthermore, when compared with Duopafei®, the DTX-PMs dramatically reduced the prostate specific antigen (PSA) level and tumor growth of prostate tumor-bearing nude mice in vivo. In conclusion, the DTX-PMs can lower systemic side effects, improve anti-tumor activity with prolonged blood circulation time, and will bring an alternative to patients with HRPC.
Animals
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Antineoplastic Agents
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pharmacokinetics
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pharmacology
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Area Under Curve
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Cell Line, Tumor
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Cell Survival
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drug effects
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Dose-Response Relationship, Drug
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Guinea Pigs
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Hemolysis
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drug effects
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Humans
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Male
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Mice
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Mice, Nude
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Micelles
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Particle Size
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Polyesters
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chemistry
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Polyethylene Glycols
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chemistry
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Prostatic Neoplasms
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drug therapy
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pathology
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Rats
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Rats, Sprague-Dawley
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Taxoids
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chemistry
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pharmacokinetics
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pharmacology
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Treatment Outcome
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Tumor Burden
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drug effects
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Xenograft Model Antitumor Assays
6.Nicotine exacerbates tacrolimus-induced renal injury by programmed cell death
Yu Ji JIANG ; Sheng CUI ; Kang LUO ; Jun DING ; Qi Yan NAN ; Shang Guo PIAO ; Mei Ying XUAN ; Hai Lan ZHENG ; Yong Jie JIN ; Ji Zhe JIN ; Jung Pyo LEE ; Byung Ha CHUNG ; Bum Soon CHOI ; Chul Woo YANG ; Can LI
The Korean Journal of Internal Medicine 2021;36(6):1437-1449
Background/Aims:
Cigarette smoking is an important modifiable risk factor in kidney disease progression. However, the underlying mechanisms for this are lacking. This study aimed to assess whether nicotine (NIC), a major toxic component of cigarette smoking, would exacerbates tacrolimus (TAC)-induced renal injury.
Methods:
Sprague-Dawley rats were treated daily with NIC, TAC, or both drugs for 4 weeks. The influence of NIC on TAC-caused renal injury was examined via renal function, histopathology, oxidative stress, mitochondria, endoplasmic reticulum (ER) stress, and programmed cell death (apoptosis and autophagy).
Results:
Both NIC and TAC significantly impaired renal function and histopathology, while combined NIC and TAC treatment aggravated these parameters beyond the effects of either alone. Increased oxidative stress, ER stress, mitochondrial dysfunction, proinf lammatory and profibrotic cytokine expressions, and programmed cell death from either NIC or TAC were also aggravated by the two combined.
Conclusions
Our observations suggest that NIC exacerbates chronic TAC nephrotoxicity, implying that smoking cessation may be beneficial for transplant smokers taking TAC.
7.Chronic hippocampus electrical stimulation for treatment of patients with temporal lobe epilepsy
Tao YU ; Guo-Jun ZHANG ; Dong-Sheng XIAO ; Yuan-Yuan PIAO ; Wei DU ; Yong-Jie LI
Chinese Journal of Neuromedicine 2013;12(12):1201-1204
Objective To evaluate the effectiveness and safety of chronic hippocampus electrical stimulation in treatment of patients with temporal lobe epilepsy.Methods The patients with temporal lobe epilepsy who were not suitable for anterior temporal lobectomy were selected.The patients had a 6-month-seizure count as baseline frequency.Medtronic electrodes were implanted directed into the amygdalohippocampal foci.After electroencephalography recording and interval stimulation for one week,implantable pulse generators were implanted and began to stimulate chronically.Seizure frequency was counted in the follow up.The efficacy and side-effect were also detected.Results Two patients were selected according to the criteria.There was no obvious side effect except for transient dizziness in one patient.One patient had a seizure reduction of>80% in a 22-month follow-up (decreasing from 3-4 times/month to 2-3 times/month); the seizure time was shorten and severity was alleviated than before treatment The other patient had a seizure reduction of>50% in a 10-month follow-up (decreasing from 6-10 times/month to 3-6 times/month); the seizure time was shorten than before treatment with few secondary generalized tonic-clonic seizures.Conclusion Chronic hippocampus electrical stimulation is a safe treatment for some patients with temporal lobe epilepsy who are not suitable for anterior temporal lobectomy; it may be helpful in controlling the seizures.
8.Distribution of High-sensitivity C-reactive Protein Status in an Urban Population in China.
Yan Bin TANG ; Jun Sheng HUO ; Jian HUANG ; Hong LI ; Jian Hua PIAO ; Jing SUN ; Li Juan WANG
Biomedical and Environmental Sciences 2020;33(6):396-402
Objectives:
To evaluate the distribution by age and sex of serum high-sensitivity C-reactive protein (hsCRP) in an urban Chinese population and to provide a profile prediction for the risk of bacterial infection, inflammatory diseases, or tissue damages in the body.
Methods:
Serum hsCRP was determined using the Roche Tina-quant immuno-turbidimetric assay on a Hitachi 7600-010 automatic biochemical analyzer (Roche Diagnostics) in 1,572 males and 1,800 females, including 78 pregnant women, who were derived from the National Health and Nutrition Survey in 2010-2012.
Results:
The average hsCRP concentration in urban China was 0.68 mg/L for males and 0.65 mg/L for females. Significant differences in hsCRP were found among different age groups ( < 0.05). Monitoring results showed no significant differences among the 6-11, 45-59, and ≥ 60-year-old groups in the comparison of hsCRP between males and females in large cities. However, hsCRP concentration was significantly higher in men aged 12-17 and 18-44 years than in women.
Conclusion
The distribution of the hsCRP status of residents in large cities in China was influenced by age and gender, and the hsCRP levels of both sexes increased gradually with age. In addition, hsCRP concentration was higher in healthy pregnant women than in non-pregnant women. Basing on our results, we recommend that this parameter be included in future national and international screening for early detection of various illnesses.
Adolescent
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Adult
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Aged
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Biomarkers
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blood
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C-Reactive Protein
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metabolism
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Cardiovascular Diseases
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epidemiology
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Child
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China
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epidemiology
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Female
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Humans
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Inflammation
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epidemiology
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Male
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Middle Aged
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Pregnancy
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Prevalence
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Urban Population
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statistics & numerical data
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Young Adult
9.Effect of Zhenzhu Tiaozhi Capsule on HbA1c in Patient with Type 2 Diabetes Mellitus: A Real-world Study
Miao-wen WU ; Xiao-qiang HUANG ; Hui-xia ZHAN ; Jin YANG ; Liu-fen PENG ; Qian LIU ; Ying-hua JIN ; Ying-yu CHEN ; Jun-jiao XU ; Si-ying LI ; Yuan-zhen MEI ; Sheng-hua PIAO ; Xiang-lu RONG ; Jiao GUO
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(19):110-117
Objective:To explore the long-term effect of Zhenzhu Tiaozhi capsule(FTZ) on hemoglobin A1c(HbA1c)in patients with type 2 diabetes mellitus (T2DM) based on real-world data. Method:T2DM patients who were provided with FTZ (FTZ group) and those receiving conventional hypoglycemic drugs (control group) were extracted from the hospital information system (HIS) of the First Affiliated Hospital of Guangdong Pharmaceutical University, followed by propensity score matching (PSM) for balancing the confounding factors between groups. With HbA1c as the efficacy evaluation index, the difference in efficacy between the two groups was compared using
10.Baicalein Mediated FAK Protein Regulates PI3K/Akt Signaling Pathway to Inhibit Proliferation and Migration of Gastric Cancer HGC-27 Cells
Dan QIAO ; Sheng-jun ZHANG ; Shi-yu WANG ; Guang-yuan YAO ; Ying-lan CAI ; Li-yan CHEN ; Ying-shi PIAO
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(7):73-80
ObjectiveTo study the possible molecular mechanism of baicalein (BAI)-mediated focal adhesion kinase (FAK) in the regulation of phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway to inhibit the proliferation and migration of gastric cancer HGC-27 cells. MethodThe gastric epithelial GES-1 cells and gastric cancer HGC-27 cells were respectively treated with BAI (0, 5, 15, 25, and 50 μmol·L-1) for 48 h, and then methyl thiazolyl tetrazolium (MTT) assay was adopted to detect effect of BAI on cell proliferation. Western blot (WB) was employed to detect the expression of FAK and the proteins related to epithelial-mesenchymal transition (EMT) and PI3K signaling pathway after intervention with different concentrations of BAI. The HGC-27 cells stably overexpressing FAK were constructed with lentivirus-mediated transfection technique, and the transfection of FAK was detected through WB and green fluorescent protein (GFP). The cells were divided into empty vector (NC) group, BAI group, FAK overexpression group, and BAI-treated FAK overexpression group, and cell proliferation activity was detected by MTT assay. The colony formation and cell migration were observed via colony formation assay and Transwell migration assay, respectively. The expression of proteins involved in EMT and PI3K signaling pathways were detected by Western blot. ResultCompared with the NC group, BAI (15, 25 and 50 μmol·L-1) inhibited the proliferation of HGC-27 cells in a dose-dependent manner (P<0.05, P<0.01) while did not affect that of GES-1 cells. BAI (5, 15 and 25 μmol·L-1) down-regulated the expression level of p-FAK (P<0.05, P<0.01). Compared with NC group, FAK overexpression group showed up-regulated expression level of FAK in HGC-27 cells. The HGC-27 cells in both NC group and FAK overexpression group had green fluorescence. Compared with NC group, BAI inhibited the growth, colony formation, and migration, while FAK overexpression promoted those of HGC-27 cells. The treatment of FAK overexpression group with BAI inhibited the enhancement of cell proliferation and migration (P<0.05). WB showed that compared with NC group, BAI (15, 25 μmol·L-1) significantly up-regulated the expression of E-cadherin protein and down-regulated that of Vimentin, Snail, p-PI3K, and p-Akt protein in HGC-27 cells (P<0.05, P<0.01). Compared with NC group, FAK overexpression group showed down-regulated expression of E-cadherin, up-regulated expression of p-FAK, Vimentin, and Snail, and increased ratios of p-FAK/FAK, p-PI3K/PI3K and p-Akt/Akt (P<0.05). This phenomenon would be reversed after BAI treatment. ConclusionBAI can affect the proliferation and migration of gastric cancer HGC-27 cells by mediating FAK to regulate PI3K/Akt signaling pathway.