1.Experimental study of human skin fibroblasts cultured in three-dimension(3D).
Zhi-guo LIU ; Jing-ning HUAN ; Yu-lin CHEN ; Sheng-de GE ; Zhi-yang FANG ; Tian-xiang OUYANG ; Xin XING
Chinese Journal of Plastic Surgery 2004;20(6):443-446
OBJECTIVETo investigate the biological characters of human skin fibroblasts in fibroblast populated collagen lattice (FPCL).
METHODSThe human fibroblasts were cultured in 3D and the collagen of the rat tail was also prepared. They were examined with the comprising cell cycle and apoptosis, mRNA expression of TGF beta1, and fibronectin, and cell morphology.
RESULTSThe flow cytometry showed that the G0/G1, stage cells were 79% +/- 3%, 87% +/- 2% after the 7 days and 14 days separately, and there were not apoptosis peak observed. RT-PCR analysis revealed that the mRNA expression of TGF beta1, and fibronectin had no difference between human skin fibroblasts cultured in 3D and 2D. Electron microscope showed the cells were plenty of chromatin and organelles.
CONCLUSIONSThe proliferation of the human skin fibroblasts in FPCL is slow, but its biological viability is better.
Animals ; Cell Culture Techniques ; Cell Division ; Cells, Cultured ; Collagen ; Extracellular Matrix ; Fibroblasts ; cytology ; Humans ; Rats ; Skin ; cytology ; Tissue Engineering ; methods
2.Value of 3D-DCE MRA in diagnosis of cavernous transformation of portal vein.
Ling-yu GE ; Sen-xiang YAN ; Shun-liang XU ; Qiang HUANG ; De-sheng SHANG
Journal of Zhejiang University. Medical sciences 2008;37(2):203-207
OBJECTIVETo evaluate three dimensional dynamic contrast-enhanced magnetic resonance angiography (3D-DCE MRA) in diagnosis of cavernous transformation of portal vein (CTPV).
METHODSTwenty-four patients with CTPV underwent 3D-DCE MRA examinations and the reconstructed images were retrospectively analyzed. A series of clinical, laboratory and imaging studies were performed on all these cases. Among all cases 14 underwent operations and 2 with hepatocellular carcinoma complicated portal thrombosis received transhepatic artery chemoembolization.
RESULTThe CTPA was located in the main trunk in 10 cases, in both the main trunk and left/right branches in 8, and in left or right branches of the portal vein in 4. In the remaining 2 cases CTPA was located at the level of superior mesenteric vein. MRA revealed multiple circuitous collateral veins striding over obstruction to extend into the liver in 9 cases,and in 7 it simultaneously showed streaky or dot-like low signal intensities representing thrombi in the extensively dilated network of portal system. MRA did not clearly demonstrate the structure of the portal vein but only showed multiple sinuous network of venous collaterals strangling together in 6 cases. In 15 cases it also showed the route and distribution of multiple hepatofugal venous collaterals.
CONCLUSION3D-DCE MRA can provide adequate information about the site and severity of CTPA.
Adult ; Aged ; Aged, 80 and over ; Contrast Media ; Female ; Hemangioma, Cavernous ; diagnosis ; etiology ; pathology ; Humans ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Liver Neoplasms ; complications ; Magnetic Resonance Angiography ; methods ; Male ; Middle Aged ; Portal Vein ; pathology ; Retrospective Studies ; Venous Thrombosis ; diagnosis ; etiology ; pathology
3.Clinical efficacy of transabdominal preperitoneal prosthesis based on inverted "T" peritoneotomy for lumbar hernia.
Si Tang GE ; He Xin WEN ; Lu Gen ZUO ; Shi Qing LI ; De Li CHEN ; Ping Sheng ZHU ; Cong Qiao JIANG ; Jie LUO ; Mu Lin LIU
Chinese Journal of Gastrointestinal Surgery 2021;24(12):1103-1106
4.Effects of montelukast on atherosclerosis and monocyte chemoattractant protein-1 expression in ahypercholesterolemic rabbit model..
Song GE ; De-Zhi LIU ; Guang-Yi ZHOU ; Wei HU ; Hong-Bing CHEN ; Guo-Dong XIAO ; Wu-Sheng ZHU ; Ge-Lin XU ; Xin-Feng LIU
Chinese Journal of Cardiology 2009;37(3):257-261
OBJECTIVETo investigate the effects of montelukast on atherosclerosis and monocyte chemoattractant protein-1 expression in a hypercholesterolemic rabbit model.
METHODSThirty four male New Zealand white rabbits were randomized into four groups including normal control group (n = 6), placebo group (n = 8), atorvastatin group (1.5 mgxkg(-1)xd(-1), beginning at 8(th) weeks for 4 weeks, n = 10) and montelukast group (1 mgxkg(-1)xd(-1), beginning at 8(th) weeks for 4 weeks, n = 10). Rabbits except those in normal control group were fed a high cholesterol diet for 12 weeks. Serum lipids were measured at 0, 8 and 12 weeks after intervention. The intima/media ratio, percentages of macrophages or smooth muscle cells in intima and the expression of MCP-1 mRNA were examined.
RESULTSAtherosclerosis was evidenced in placebo group and atorvastatin or montelukast treatment significantly reduced neointima (0.32 +/- 0.12 and 0.34 +/- 0.10 vs. 1.12 +/- 0.36, P < 0.05) and macrophage content [(9.8 +/- 4.6)% and (11.2 +/- 3.7)% vs. (34.6 +/- 8.8)%, P < 0.05], increased SMC content [(18.6 +/- 6.9)% and (19.2 +/- 8.6)% vs. (5.2 +/- 2.3)%, P < 0.05] and inhibited expression of MCP-1 mRNA (0.42 +/- 0.08 and 0.40 +/- 0.06 vs. 2.36 +/- 0.48, P < 0.01). Montelukast had similar anti-atherogenetic effects as atorvastatin but had no influence on plasma lipids.
CONCLUSIONSMontelukast could attenuate atherosclerosis in this hypercholesterolemic rabbit model which might be attributed to its anti-inflammatory effects.
Animals ; Atherosclerosis ; metabolism ; Chemokine CCL2 ; metabolism ; Hypercholesterolemia ; Macrophages ; metabolism ; Rabbits ; Tunica Intima
5.Protective role of MMP-9 inhibitor batimastat in acute lung injury after cardiopulmonary bypass.
Sheng-lin GE ; Wen-hui GONG ; Cheng-xin ZHANG ; Lei ZHANG ; Pei-hua HAN ; Sheng-quan ZHANG ; Jun-bo FENG ; De-cun ZHOU
Chinese Journal of Surgery 2010;48(1):57-61
OBJECTIVETo investigate the protective effect of exogenous inhibitor of matrix metalloproteinases-9 (MMP-9), batimastat, in the lung injury induced by cardiopulmonary bypass (CPB) in dogs.
METHODSThirty healthy mongrel puppies were randomly divided into 3 groups: control group, low-dose group [batimastat 10 mg/(kg.d) for 3 days before operation] and high-dose group [batimastat 30 mg/(kg.d) for 3 days before operation]. The off-pump puppies' model of acute lung injury was established, and hemodynamic and respiratory parameters were monitored. The preoperative and postoperative alveolar-arterial oxygen difference (A-aDO(2)) and respiratory index (RI) were calculated. From the beginning of surgery, blood samples were taken at the time 0, 60, 120, and 270 min. Plasma concentrations of MMP-9 were measured by ELISA, and blood MMP-9 mRNA expressions were determined by RT-PCR. The myeloperoxidase (MPO) activity of centrifugal bronchoalveolar lavage fluid were measured by Colorimetry. And MMP-9 activity was determined by Gelatin zymography. Light and electronic microscope were used to observe the morphological changes of lung tissue. A small piece of left lung tissue was taken, weighed and baked to calculate the wet weight (W/D) index.
RESULTSAfter cardiopulmonary bypass, the concentrations of MMP-9 and mRNA expressions of the control group were increased significantly, and lung injury was apparent. At 270 min, the MMP-9 plasma concentration of high-dose group (17.36 +/- 1.18) microg/L was significant reducing than control group (30.47 +/- 2.22) microg/L (P < 0.05). After operation, A-aDO(2) and RI of high-dose group were significantly improved than control group (P < 0.05). The W/D index of the high-dose group (2.8 +/- 0.48) was significantly lower than that of control group (4.7 +/- 0.6) (P < 0.05). And the pathological changes of lung tissue were significantly improved in the high-dose group. However, there was no significant difference in the MMP-9 mRNA expression in three groups.
CONCLUSIONSBatimastat plays a role in the protection of the lung injury of CBP by reducing the concentration and activity of MMP-9, the degradation of the cell membrane and pulmonary neutrophil infiltration and reduction of pulmonary edema.
Acute Lung Injury ; etiology ; prevention & control ; Animals ; Cardiopulmonary Bypass ; Disease Models, Animal ; Dogs ; Lung ; pathology ; Matrix Metalloproteinase 9 ; metabolism ; Matrix Metalloproteinase Inhibitors ; Phenylalanine ; analogs & derivatives ; pharmacology ; Postoperative Complications ; prevention & control ; Thiophenes ; pharmacology
6.Multi-center study of clinical treatment on the flat type of sudden hearing loss.
Guo-hao CHEN ; Rong ZHANG ; Ying-ge WANG ; Sheng-nan YE ; Chang LIN ; Jin-mei CHENG ; Jian-xin QIU ; Kai-le WU ; De-zhi YU ; Bu-sheng TONG ; null
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2013;48(5):374-378
OBJECTIVETo investigate the effect of different treatment options for the flat type of sudden hearing loss.
METHODSProspective, multi-center clinical study was carried out using internationally used standardized clinical research method. Patients with the flat type of sudden hearing loss between 18 and 65 years old, within two weeks duration, and without any medical treatment were recruited. Treatment options were randomly selected according to the designed random table.
RESULTSFrom August 2007 to October 2011, 402 patients with the flat type of sudden hearing loss who met the criteria (account for 39.26% of the total number of patients) from the 33 hospitals were collected; the total effective rate was 82.59%, and no significant difference was detected between different treatments, (χ(2) = 10.95, P = 0.28). In the 402 cases, 139 were cured (34.58%); 118 were markedly improved (29.35%); 75 were effective (18.66%); 70 were invalid (17.41%).
CONCLUSIONSThe therapeutic efficacy of flat type of sudden hearing loss overall is good; the treatment of improving the inner ear blood rheology and/or reducing blood fibrinogen has clinical significance; the therapeutic efficacy of using glucocorticoid systemically is good as well; there is no obvious difference between combination and single medication.
Adult ; Aged ; Ear, Inner ; Glucocorticoids ; therapeutic use ; Hearing Loss, Sudden ; therapy ; Humans ; Middle Aged ; Prospective Studies ; Treatment Outcome ; Young Adult
7.Effect of qubi zhentong recipe on synovial gene expression in collagen-induced arthritis in rats.
Jian-ming YU ; Yun-qing WANG ; Xi-de LIU ; Pi-sheng QU ; Fan TAO ; Qing-ge GUO
Chinese journal of integrative medicine 2013;19(10):749-754
OBJECTIVETo investigate the effect of the Chinese medical formula Qubi Zhentong Recipe(, QZR) on the synovial gene expression profile in collagen-induced arthritis (CIA) rats.
METHODSTen rats were randomly chosen from 60 rats as the control group, and the other 50 rats were used for the CIA models. The CIA model group was constructed by bovine injection of type II collagen through the rats' neck and tail. Twenty rats were randomly chosen from 34 successful CIA models and randomly assigned into two groups: the model group (n =10) and the QZR group (n=10). The QZR group was fed intragastrically with QZR 22.9 g/(kg·d) (10 times the clinical adult dose), and the CIA model group was given the same dose of normal saline. Both model and QZR groups were administered treatment once a day. Total RNA was collected from the knee joint synovium after 30 days. The change in gene expression profile was analyzed by a whole gene chip.
RESULTSA total of 76 genes showed a difference in expression between CIA model group and the control group; 35 genes were down-regulated and 41 were up-regulated. A total of 67 genes showed a difference in expression between the model group and the QZR group; 48 genes were down-regulated and 19 were upregulated.
CONCLUSIONSQZR may affect CIA by stimulating multiple genes and targets, which are related to oncogenes, apoptosis, metabolism, the immune system, ion channels, and transport proteins.
Animals ; Arthritis, Experimental ; genetics ; Cattle ; Disease Models, Animal ; Down-Regulation ; drug effects ; genetics ; Drugs, Chinese Herbal ; pharmacology ; Electrophoresis, Agar Gel ; Extremities ; pathology ; Gene Expression Regulation ; drug effects ; Male ; Rats ; Rats, Wistar ; Synovial Membrane ; drug effects ; metabolism ; pathology ; Transcriptome ; Up-Regulation ; drug effects ; genetics
8.Erythroleukemia - a subtype of myelodysplastic syndrome?.
Yi NIU ; Shu-Chang CHEN ; Bin JIANG ; De-Gao LI ; Chang-Wen GE ; Rong-Sheng LI
Journal of Experimental Hematology 2007;15(2):219-223
In order to study whether erythroleukemia was really a subtype of acute leukemia, the clinical laboratory characteristics and development of disease in 21 cases of erythroleukemia were analyzed. The results indicated that the percentage of patients with leucocytopenia, anemia and thrombocytopenia were 42.9%, 81% and 81% respectively at the time of diagnosis. These were 85.7% of patients with myelocytes and premonocyte, 52.4% of patients with erythroblast in their blood smear respectively. All of the bone marrow showed active or significantly active proliferation. The median percentage of erythro-lineage, myeloblast of NEC and displasia were (58.3 +/- 8.0)%, (58.0 +/- 18.4)% and 66.7% respectively, that is different from typical AML. 52.4% of M(6) patients transferred to RAEB/RAEB-T and AML-M(2) subtype in the disease progression. 11/19 cases (57.4%) achieved remission (CR 10; PR 1) after chemotherapy. The median remission length were 6 months for CR patients and 2 months for PR patients, but most of CR patients displayed obvious displasia of bone marrow and cytopenia of blood in the period of CR. The median survival length of M(6) and MDS-->M(6) from time of diagnosis were 13.0 +/- 13.2 and 2.3 +/- 1.3 months respectively. It is concluded that there are differences between M(6) and typical AML. Most of M(6) patients would rather be classified MDS RAEB and RAEB-t with over-hyperplasia of erythron lineage than a subtype of AML.
Adult
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Aged
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Bone Marrow Examination
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Diagnosis, Differential
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Female
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Humans
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Leukemia, Erythroblastic, Acute
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blood
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diagnosis
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Male
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Middle Aged
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Myelodysplastic Syndromes
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classification
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diagnosis
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Retrospective Studies
9.Results of myasthenia gravis treated with thymectomy.
Hai-tao ZHANG ; De-ruo LIU ; Yong-qing GUO ; Bing-sheng GE ; Yan-chu TIAN ; Bin SHI ; Chao-yang LIANG
Chinese Journal of Surgery 2007;45(22):1546-1548
OBJECTIVETo evaluate the effectiveness of thymectomy for myasthenia gravis (MG) and the relative risk factors for postoperative myasthenic crisis.
METHODSThe clinic data of 78 cases with MG who underwent thymectomy from June 1985 to June 2005 were analyzed retrospectively. The relative risk factors of postoperative myasthenic crisis were analyzed and the differences between new and old region of perioperative management were compared.
RESULTSThe symptom of MG was complete remission in 21 cases, significantly improved in 38 cases, improved in 11 cases and unchanged in 8 cases, respectively. The symptom duration before operation, preoperative serum level of anti-acetylcholine receptor antibody, Osserman stage and pathological type of thymoma were independent relative risk factors for postoperative myasthenic crisis. The new region of perioperative management was significant better than the old one.
CONCLUSIONSurgical treatment shows significant clinical benefits for patients with MG.
Adolescent ; Adult ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Muscle Weakness ; etiology ; prevention & control ; Myasthenia Gravis ; surgery ; Postoperative Complications ; prevention & control ; Retrospective Studies ; Thymectomy ; adverse effects ; methods ; Treatment Outcome
10.Clinical observation of the long-term effects of rhEGF on deep partial-thickness burn wounds.
Guang-yi WANG ; Zhao-fan XIA ; Shi-hui ZHU ; Hong-tai TANG ; Jing-ning HUAN ; Yu-lin CHEN ; Sheng-de GE
Chinese Journal of Burns 2003;19(3):167-168
OBJECTIVETo evaluate the safety and long-term effect of recombinant human epithelial growth factor (rhEGF) on deep partial-thickness burn wounds.
METHODSThirty-seven burn patients were enrolled in this study and were observed by randomized, double-blinded and placebo-controlled protocol. An area of deep partial-thickness burn wounds from each patient was divided into control (C) and treatment (T) portions. The wound in C was treated with normal saline while that in T with rhEGF. The patients were followed-up for 1 and 4 years after wound healing. The healed wounds were evaluated by modified Vancouver scar scale in terms of scar index (SI).
RESULTS1 year after wound healing, it was found that the SI in T group (7.19 +/- 1.67) was obviously lower than that in C group (8.92 +/- 1.78, P < 0.01). The SI in T group (6.12 +/- 1.54) was still evidently lower than that in C group (8.09 +/- 1.81, P < 0.01) four years after wound healing. There were no signs of development of tumor or cancer in all the tested burn wound areas.
CONCLUSIONExternal application of rhEGF might be beneficial to the healing quality of deep partial-thickness burn wound with less scar formation and better long-term effects, and it is safe.
Adult ; Burns ; drug therapy ; Double-Blind Method ; Epidermal Growth Factor ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Male ; Recombinant Proteins ; therapeutic use ; Treatment Outcome ; Wound Healing ; drug effects ; Young Adult