2.Human leukocyte antigen (HLA)-haploidentical hematopoietic stem cell transplantation for 8 patients with leukemia and review of the literature.
Xin-Sheng XIE ; Ding-Ming WAN ; Hui SUN
Chinese Journal of Oncology 2010;32(8):636-637
Adolescent
;
Adult
;
Female
;
Graft vs Host Disease
;
etiology
;
prevention & control
;
HLA Antigens
;
immunology
;
Haplotypes
;
Hematopoietic Stem Cell Transplantation
;
adverse effects
;
methods
;
Histocompatibility Testing
;
Humans
;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
;
therapy
;
Leukemia, Myeloid, Acute
;
therapy
;
Male
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
therapy
;
Survival Analysis
;
Young Adult
3.Effectiveness and safety of acupuncture for supraventricular tachycardia: a systematic review and meta-analysis.
Wan-xin WEN ; Xian-sheng LI ; Xin-feng GUO ; Li ZHOU ; Wei-hui LV
Chinese Acupuncture & Moxibustion 2014;34(11):1146-1150
The effectiveness and safety of acupuncture for the treatment of supraventricular tachycardia were systematically reviewed. The randomized controlled trials (RCTs) regarding acupuncture for supraventricular tachycardia were searched in domestic and overseas databases, and the evaluation tool of bias risk in Cochrane Handbook 5.1.0 software was used to perform the evaluation of bias risk in literature, and RevMan 5.2 software was applied for statistics and Meta-analysis. Five RCTs involving 323 patients were included. The results showed that compared with the blank control group, the acupuncture reduced the heart rate by 18.8 times/min [95% CI (12.68, 24.92)]; the clinical effective rate in the acupuncture group was superior to that in the diltiazem group [OR= 3.11, 95% CI (1.50, 6.46)]; the difference of immediate effect between propafenone and acupuncture was not significant. No reports regarding adverse events was described in 5 RCTs. As was shown in the present evidence, acupuncture is safe and effective for the treatment of supraventricular tachycardia, but the level of evidence was low and the intensity of conclusion needed to be improved.
Acupuncture Points
;
Acupuncture Therapy
;
adverse effects
;
Humans
;
Randomized Controlled Trials as Topic
;
Tachycardia, Supraventricular
;
physiopathology
;
therapy
;
Treatment Outcome
4.Establishment and application of an in vitro mouse intestinal organoid culture system
sheng Guang WAN ; yan Xin ZHANG
Acta Laboratorium Animalis Scientia Sinica 2017;25(5):513-518
Objective To establish a small intestinal organoid culture system as an in vitro study model of intesti-nal epithelial cells, and to explore the relevant pathological detection techniques and provide a convenient platform for in vitro study of various intestinal diseases. Methods The mouse intestinal epithelium was isolated and cultured into or-ganoids to simulate the growth and development of intestinal epithelium in vitro. The proliferation and differentiation signals were detected by immunohistochemistry and three-dimensional immunofluorescence technique. Results The culture system of the mouse small intestine epithelium was established. Immunohistochemical staining and three-dimensional immunofluo-rescence technique were successfully used to detect the growth and development of small intestinal organoids. Conclusions The successfully established mouse small intestinal organoid culture system and application of immunoassay technology will gradually become a most favorable technical means for studies of various intestinal diseases.
7.Killing effect of double suicide genes mediated by retroviral vector on k562 cells.
Yi-Rong JIANG ; Ying-Chang LAI ; Xiao-Lin CHEN ; De-Sheng WAN ; Wan-Ning CHEN ; Miao-Hua QI ; Chun-Sheng LIU ; Xue-Liang CHEN ; Dao-Xin MA
Journal of Experimental Hematology 2007;15(1):47-51
The aim of study was to investigate the killing effect of double suicide gene system mediated by retroviral vector on K562 cells in vivo and ex vivo. CDglyTK gene was transfected into PA317 cells by using lipofectamine. K562 cells were infected with viral supernatant. K562/CDglyTK cells were treated with 5-fluorocytosine (5-FC) and/or ganciclovir (GCV). Mice were randomly divided into three groups: tumor formation, tumor inhibition and tumor therapy. Each mouse was implanted with K562/CDglyTK cells or K562 cells. The results indicated that the killing effect of 5-FC in combination with GCV on K562/CDglyTK was more significant than using 5-FC or GCV alone. In vivo study showed that after being injected subcutaneously with K562 cells and K562/CDglyTK cells, there was not obvious difference in tumor formation rate of mice, 5-FC + GCV could suppress tumor formation of the K562/CDglyTK cells. After being treated with 5-FC and GCV, the median tumor volume of mice implanted with K562/CDglyTK cells decreased obviously, compared with the control group. Their median survival was significantly prolonged. It is concluded that double suicide genes are more effective for killing effect on K562 cells in vivo and in ex vivo. It may be applicable to clinical gene therapy.
Cytosine Deaminase
;
genetics
;
Flucytosine
;
pharmacology
;
Ganciclovir
;
pharmacology
;
Genes, Transgenic, Suicide
;
genetics
;
Genetic Therapy
;
Genetic Vectors
;
genetics
;
Humans
;
K562 Cells
;
Protein-Tyrosine Kinases
;
genetics
;
Receptor Protein-Tyrosine Kinases
;
biosynthesis
;
genetics
;
Recombinant Fusion Proteins
;
genetics
;
Recombination, Genetic
;
Retroviridae
;
genetics
8.Kinetic patterns of cytokine expressions in H-2 haploidentical nonmyeloablative bone marrow transplantation in mice.
Xin LI ; Wan-Jun SUN ; Ye YUAN ; Ning MAO ; Hui-Sheng AI
Journal of Experimental Hematology 2005;13(4):687-691
In order to explore what role relative cytokines play in acute GVHD (aGVHD) of mice transplanted with H-2 haploidentical nonmyeloablative bone marrow, a murine model was established by using C57BL/6J mouse as donor and (C57BL/6J x BALB/C) F1 mouse as the recipient. The recipient mice were given CsA and mycophenolate mofetile (MMF) for prophylaxis of aGVHD. The expression levels of IL-2, INFgamma, IL-4 and IL-10 in the spleen were detected by semi-quantitative RT-PCR at different time points after transplantation. The results showed that the expression levels of these cytokines increased slightly in the group only received nonmyeloablative conditioning. The expression levels of IL-2 and INF-gamma elevated significantly after transplantation in group 2 (without aGVHD prophylaxis), peaked at day 21 and day 14 respectively, then dropped rapidly, returned to the basal levels at day 35. The study on kinetic pattern of expression of IL-2 and INF-gamma in group 3 (with aGVHD prophylaxis) gave a similar result to group 2, but the peak value of cytokine expression in group 3 was much lower than that in group 2. The expression of IL-4 in Group 2 and group 3 all peaked at day 14, but the peak value in group 3 was higher than that in group 2, and decreased slowly in group 3. The expression of IL-10 increased gradually after transplantation, peaked at day 14, decreased after day 21, elevated again until day 35. It is concluded that the expression levels of these cytokines in the spleen all increase after H-2 haploidentical nonmyeloablative bone marrow transplantation. CsA and MMF can reduce the incidence and severity of aGVHD by down-regulating the expression levels of IL-2 and INF-gamma.
Animals
;
Bone Marrow Transplantation
;
immunology
;
methods
;
Cyclosporine
;
administration & dosage
;
Cytokines
;
genetics
;
Gene Expression
;
drug effects
;
Graft vs Host Disease
;
prevention & control
;
H-2 Antigens
;
genetics
;
Interferon-gamma
;
genetics
;
Interleukin-2
;
genetics
;
Kinetics
;
Mice
;
Mice, Inbred BALB C
;
Mice, Inbred C57BL
;
Mycophenolic Acid
;
administration & dosage
;
analogs & derivatives
;
Reverse Transcriptase Polymerase Chain Reaction
9.TF/F Ⅶa complex induce the expression of MMP-7mRNA via P38 signal pathway in LOVO cells of colon cancer in vitro
Jian-Quan ZHANG ; Yuan-Lian WAN ; Yu-Cun LIU ; Xin WANG ; Jian-Qiang TANG ; Tao WU ; Jing ZHU ; Yi-sheng PAN
Chinese Journal of General Surgery 2000;0(12):-
Objective To investigate the expression of matrix metalloproteinase 7(MMP-7) mRNA in LOVO cells of colon cancer induced by TF/F Ⅶ a and its signal pathway.Methods We transfected LOVO cells stably with RNAi plasmid targeting to tissue factor to get TFRNAi LOVO cells and detected efficiency of interference in TFRNAi LOVO cells based on Western blot analysis;Expression of MMP-7 was evaluated in LOVO cells treated with 100 nmol/L FⅦa in 0 h、4 h、8 h、12 h、24 h based on RT-PCR and Northern blot.Expression of MMP-7mRNA was determined in quiescent LOVO cells treated with different doses of FⅦa(0 nmol/L、10nmol/L、50 nmol/L、100 nmol/L、200 nmol/L)for 8 h based on Northern blot.Quiescent LOVO cells were treated for 0 h、4 h、8 h、12 h、16 h、24 h with 100 nmol/L FⅦa to evaluate the expression of p-P38;The expression level of MMP-7mRNA induced by 100 nmol/L FⅦa for 8 h in LOVO cells blocked by 10retool SB203580 0.5 h previously and in TFRNAi LOVO cells were measured by Northern blot.Results Northern blot analysis revealed that FⅦa markedly increased the expression of MMP-7mRNA in a time-and dose-dependent manner.Western blot analysis confirmed that FⅦa stimulates p-P38 in a time-dependent manner.SB203580 block 59.2% expression of MMP-7mRNA in LOVO cells induced by TF/FⅦa.In TFRNAi LOVO cells,the expression of MMP-7mRNA induced by TF/FⅦa was 48% less than that in normal LOVO cells.Conclusions TF/FⅦa Complex induces the expression of MMP-7mRNA in LOVO cells in vitro,possibly through P38 pathway.
10.Investigation of increasing efficacy of aterial infusion of NaHCO3 to solid malignant tumors in interventional chemotherapy
Ping-Sheng FAN ; Yu-Xiu WAN ; Ji-De LI ; Hu BEI ; Ke-Hai FENG ; Shi-Ceng WANG ; Xin-Min LI ; Ru-Hua LIU ; Li-Yuan HU ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
Aim To observe the effect of interventional therapy of 5-fluorouracil(5-Fu), mitomycin C (MMC) and adriamycin (ADM) on solid malignant turmors in association with aterial infusion of NaHCO3.Methods Patients were randomly divided into two groups.With Seldinger technique,through the femoral atery to tumor atery.The patients in the control group were infused by anticarcinoma agents simply ,and patients in the treatment group were initially infused by NaHCO3,and then by NS 30 ml and anticarcinoma agents seperately. Results Partial remission (PR) in the group treated with NaHCO3 and anticarcinoma agents was significantly higher than in the group treated simply with anticarcinoma agents.Conclusion Aterial infusion of NaHCO3 into malignant tumors can increase the efficacy of ADM,MMC and 5-Fu.