1.Study on Hydrolysis of chitosan by Lysozyme
Sunying ZHOU ; Ping YU ; Sheng CHEN
Chinese Journal of Marine Drugs 2000;0(06):-
The effects of reaction temperature, pH, substrate and enzyme concentration, deacetylation degree of chitosan on the hydrolytic velocity of chitosan by Lysozyme were investigated. The results showed that the specificity of catalyst obey the dynamics of michae-lis-Menten. The optimum temperature and pH were 50℃ and 6. 0 respectively, the reaction velocity was reduced while the deacetylation degree of chitosan was raised. The mean molecular weight of low molecular chitosan has been determined by high performance liquid chro-matography method, the mean molecular weight was 8328.
4.Clinical characteristics of Mallory-Weiss syndrome in children.
Long XU ; Bao-ping YU ; He-sheng LUO
Chinese Journal of Pediatrics 2005;43(10):791-792
Adolescent
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Child
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Child, Preschool
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Female
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Humans
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Male
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Mallory-Weiss Syndrome
5.Overexpression and RNAi vectors built for key secondary metabolic pathway genes PAL, HMGR, PGT of Arnebia euchroma.
Teng XIE ; Yu-Zhong LIU ; Sheng WANG ; Tan LIU ; Li-Ping KANG ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2014;39(23):4538-4543
Arnebia euchroma is the main source for medicinal herb Zicao. and its most important component shikonin compounds have high medicinal and industrial value. This research is aimed to build overexpression vectors and RNAi vectors for key secondary metabolism genes of A. euchroma, and bulid platform for constructions of related transgenic lines using GATEWAY technology. To build genetic material based genetic research platform is to provide a great convenience for digging and functional verification of the genes on secondary metabolic pathway, and also to fill the gaps in transgenic research of A. euchroma. This study is also important for the cultivation of shikonin high-yielding strains of A. euchroma.
Boraginaceae
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genetics
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metabolism
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Genetic Vectors
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genetics
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metabolism
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Plant Proteins
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genetics
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metabolism
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RNA Interference
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Secondary Metabolism
6.Application of Three-Dimensional Computed Tomography for Detecting Femoral Neck Anteversion in Development Dislocation of Hip in Children
ke, SUN ; sheng-ping, TANG ; jun-chang, QIN ; wei, YU ; jing-ming, HAN ; bao-ping, LIU
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To evaluate the value of reconstruction of three-dimentional CT in development dislocation of hip(DDH)in children.Methods Twelve cases of DDH concluded 4 bilateral and 8 unilateral cases.To sum up,16 sick hips were operated and 8 normal hips were also obtained by three-dimensional CT(Hip speed Fi/x,GE Co).Results 3D reconstruction were used to show femoral head,(acetabulum) and relationship of acetabulum and femoral head respectively.The difference between FNA measurement of sick hips and those of normal hips were significant(P
7.Clinical Epidemiologic Studies on Children with Transient Synovitis of Hip
ke, SUN ; sheng-ping, TANG ; wei, YU ; bao-ping, LIU ; jing-ming, HAN ; jun-chang, QIN
Journal of Applied Clinical Pediatrics 1993;0(03):-
Objective To investigate the clinical epidemiologic features of transient synovitis(TS) of hip in children occurred in Shen-zhen district.Methods The medical files were reviewed and a standard questionnaire was filled according to the conditions of 705 cases such as pathogeny,clinical manifestation,therapy and prognosis.Results Transient synovitis occurred in a sporadic form all the year round.The peak age of patients with TS was 3-7 years old.The ratio of boys to girls was 2.9:1.About 19.3% patients were attacked an upper respiratory tract infection and 11.9% patients attributed the symptoms to trauma or severe activities before 1 week.A varying degree of painful limp and restriction of movement at the hip were found clinically.All of cases were cured by skin traction.The incidence of recurrence was 6.95%.Conclusions Male predominance is found in TS.It is characteristic of sporadic form in the 4 seasons and intently relation to an upper respiratory tract infection and trauma or severe activities.TS is recurrent and the prognosis is good by skin traction.
8.Treatment of GartlandⅢ Supracondylar Fracture of Humerus in Children by Overhead of Olecranon Traction
ke, SUN ; sheng-ping, TANG ; wei, YU ; bao-ping, LIU ; jing-ming, HAN
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To discuss the indication and complication of overhead traction of olecranon for displaced extension-type GartlandⅢ supracondylar fracture of the humerus in children.Methods Total of 87 patients(Gartland Ⅲ) proceeded with overhead skeletal traction of ulnar olecranon,including 68 cases of ulnar deviation and 19 cases of radial deviation.Eighteen cases had complicating revolve deviation.According portable X-ray results in the 2nd and 5th,three-dimension adjustment was performed within 1 week.Five patients were treated by open reduction because of symptoms nerves and blood vessel compression.When X-ray examination showed good callus formation and stable reduction,the patient was discharged after the elbow was stabilized in flexion position with plaster fixation,which was removed in 2 weeks.Results Sixty-seven of 87 patients were followed-up for 18 months.All of them had excellent results except one who had permanent ulnar nerve injury.No cubitus varus was observed.Conclusions The overhead olecranon skeletal traction is a simple,effective method,because it can increase joint motion,relieve elbow swell and pain rapidly,and improve upper limb line of traction.However,open reduction shall be done promptly if there is evidence of nerve,blood vessel or soft tissue between broken ends of fractured bone within 5 days.
9.Therapeutic effects of artesunate on cytomegalovirus pneumonia in mice
Ping WANG ; Sheng CHEN ; Xiao-tao HUANG ; Xiao-mei XIAO ; Qing-ping ZHAN ; Ai-ping QIN ; Xi-yong YU
Acta Pharmaceutica Sinica 2020;55(11):2651-2656
To investigate the therapeutic effect of artesunate on mouse cytomegalovirus pneumonia, the BALB/c-nu mice were infected with murine cytomegalovirus-green fluorescent protein (MCMV-GFP) by nose dropping method. The experimental protocol was approved by the Medical Laboratory Animal Ethics Committee of Guangzhou Medical University. The BALB/c-nu mice were randomly divided into five groups: control group, MCMV pneumonia group, and artesunate (60, 120, and 240 mg·kg-1) groups. The survival rate, weights, and virus loads in lungs among the groups were observed. The degree of histopathologic changes in lungs was assessed directly by hematoxylin-eosin (HE) assay. MCMV-GFP expression was assessed by immunofluorescence. In addition, reverse transcription polymerase chain reaction (RT-PCR) analysis was performed to investigate the content of major immediate early 1 (
10.Study on extraction technics and variety trends of the flavonoids in Sarcandrae glabra.
China Journal of Chinese Materia Medica 2007;32(4):307-309
OBJECTIVETo study on extraction technics and variety trends of the flavonoids in Sarcandrae glabra.
METHODBy using S. glabra in Guizhou as materials and the experiment of L9 (3(4)) by ethanol as extracting way, The variety trends of the flavonoids from S. glabra are studied under the optimum extracting conditions during different raw stored times, different gathering season, different S. glabra position.
RESULTThe flavonoid's contains was more low when the raw material was stored more long and descended average 24.74% annually. The flavonoid's contains had the tallest in 10-12 month by gathering indifferent season. The flavonoids contains were the tallest in the leaf of S. glabra in Guizhou than in the root and stem, and the contains were the lowest in stem.
CONCLUSIONThe optimum conditions of extracting flavonoids from S. glabra are obtained when the condition is 60% of density of ethanol at 10 times of volume, 80 degrees C of extracting temperature, 3 h of extracting hours, 3 times of extracting number of times in 10-12 month, in the leaf, the raw material was stored more shortest, the flavonoid's contains had the tallest.
Ethanol ; chemistry ; Flavonoids ; analysis ; isolation & purification ; Magnoliopsida ; chemistry ; Plant Leaves ; chemistry ; Plant Roots ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Seasons ; Technology, Pharmaceutical ; methods ; Temperature ; Time Factors