Objective To investigate the effects of propofol on activation of NF-κB in polymorphonuclear neutrophils (PMNs) in rats with LPS-induced acute lung injury (ALI). Methods Sixty healthy SD rats of both sexes, aged 3 months, weighing 250-350 g, were randomly divided into 5 groups (n = 12 each):control group (group C), ALI group and 3 different dose of propofol groups (group P1, P2, P3). The animals were anesthetized with intraperitaneal 3% pentobarbital sodium 40 mg/kg. LPS 5 mg/kg was injected via femoral vein in group ALI.Propofol 5, 10 and 15 mg· kg- 1· h- 1 was infused intravenously over 2 h immeliately after injection of LPS 5 ng/kg through femoral vein in group P1, P2 and P3 respectivey. In group C normal saline 10 ml was injected via femoral vein instead. All rats were killed by exsanguination at the end of infusion of propofol. The right lung was removed for microscopic examination. The morphologic changes were scored 0-3 (0 = normal, 3 = severe morphologic changes). Blood samples were collected from carotid artery for determination of the expression of total NF-κB and activated NF-κB in PMNs by Western blot. Results Compared with group C, morphologic change scores and activated NF-κB expression in PMNs were significantly increased in group ALI, P1 and P2, and morphologic change scores increased in group P3. Morphologic change scores in group P1 and P2 and activated NF-κB expression in PMNs in group P1, P2 and P3 were significantly decreased compared with those in group ALl. Morphologic change scores and activated NF-κB expression in PMNs were decreased gradually in group P1, P2 and P3 . There was no significant difference in total NF-κB expression in PMNs among all groups. Conclusion Propofol can attenuate ALI induced by LPS through inhibition of the activation of NF-κB in PMNs in rats.

Objective To observe the effect of intravenous injection of different doses of propofol on the ceil apoptosis and NF-kB p65 in the acute lung injury(ALl)induced by LPS in rats.Method Sixty SD rats were randomly(random number)divided into five groups,namely,control(NS)group,Au model group and propofol intervention groups(P1,P2,P3 groups).The lung injury was evaluated by using microscopy with hematoxylin and eosin(HE)staining and arterial blood gas,and Western blotting Was applied to evaluating the nuclear translocation of NF-KB P65 in lung tissues.The apoptosis rate of lung tissue Was determined by flow cytometric analysis.Results Lung injury in model group reached the pathologic criteria of acute lung injury,and it was attenuated apparently in propofol intervention groups(P1,P2,P3 groups)in dose-dependent manner.Western blotting results showed that the nuclear translocation of NF-KB P65 and the apoptosis rate increased significantly in ALI model group compared with control group(P<0.05),and decreased in propofol intervention groups compared with ALl model group(P<0.05).Conclusions Propofol Can attenuate acute lung injury induced by LPS in rats,and significantly inhibit the nuclear translocation of NF-KB P65 and the cell apoptosis in lung tissues.The effect of propofol attenuating acute lung injury induced by LPS in rats may be attributed to the inhibition of nuclear translocation of NF-KB P65and ceil apoptosis in lung tissues.

Objective To explore an optimal treatment and to study the prognosis related factors of breast cancer patients with local recurrence after mastectomy. Methods From 2002. 7 to 2005. 2, 477female patients with loco-regional recurrence of breast cancer treated in Tianjin Cancer Hospital were analyzed retrospectively. Results In 477 cases, recurrence within 1 year after mastectomy accounted for 26. 2% , recurrence within 2 years accounted for 61. 2%. There were 310 cases with metastasis after local recurrence was diagnosed, accounting for 65.0%. 5-year total survival rate after recurrence is 48.4%.Local control rates varied in subgroups with different recurrence site, clinical subtypes, radiotherapy fields,with or without radiotherapy, surgical resection or excisional biopsy ( P<0. 05 ). There was a statistical difference in distant metastasis rate and 5-year survival rate among subgroups which had different clinical stage of primary tumor, disease-free interval, clinical subtypes or treatment methods ( P<0. 05 ). Simplistic treatment option, late clinical stage of primary tumor and triple-negative breast cancer were the independent factors predicting poor prognosis for recurrent breast cancer ( P<0.05 ). Conclusions Multi-site recurrence and triple-negative breast cancer lead to a poor local control. Local expansion of radiotherapy combined with surgery improves the local control rate. Patients with late clinical stage of primary tumor,recurrence within 2 years, triple-negative breast cancer are likely to have distant metastasis when recurrence is diagnosed. Combined treatment program improves survival rate.

Genomics ; Medicine, Chinese Traditional

This article was aimed to study the factors associated with sleep quality of anger-out and anger-in population based on the frequent pattern-tree (FP-Tree) growing algorithm with data mining. The algorithm of structuring frequent model FP-tree and mining frequent itemsets were designed. The database information scanned was recorded by using FP-Tree growing algorithm through state-trees. The frequent itemsets met minimum support required was generated through reducing the search space of project sets and scanning database only one. The data mining of all factors associated with emotional diseases was actualized. The results showed that factors associated with sleep quality of anger-out and anger-in population were disturbance in respiration, cough or snoring, feeling cold, hot or nightmares. The total time for program analysis was 2 seconds. It was concluded that data mining algorithm based on FP-Tree frequent itemsets can effectively realize the useful information receiving from factors associated with emotional diseases.

The ideal molecular imaging probe should have characteristics of specificity,sensitivity and safety.Aptamers are sing-strand DNA or RNA oligonucleotides which can bind their targets with high specificity and high affinity.Aptamers are new types of molecular imaging probe because they have many advantages,such as their large sum of possible targets,small molecule weights,easily production and modification,low immuno-genicity,high tissue penetration and so on.Now more and more aptamers research on tumor molecular imaging provides a new technology and tool for tumor diagnosis and therapy.

ObjectiveTo explore the correlation between open field test(OFT) and light-dark box (LDB),as two animal models of state anxiety in Kunming mice.MethodsThe behavior of adult,male,Kunming mice in OFT and LDB was recorded by sequence,for five minutes,with a one-week inter-trial interval.The following parameters were evaluated:percentage of time exploring in the OFT central area( OFT-Ctime％ ) ; percentage of squares crossing in the OFT central area( OFT-Ccross％ ) ; total number of squares crossing in OFT(OFT-Cross) ;total number of rears in the OFT(OFT-Rear) ; number of fecal boli in OFT(OFT-FB) ; percentage of time exploring in the LDB light area( LDB-Ltime％ ) ; percentage of squares crossing in the LDB light area( LDB-Lcross％ ) ;and percentage of rears in the LDB light area( LDB-Lrear％ ) ; transitions between two areas in LDB( LDB-Transition) ; total number of squares crossing in LDB(LDB-Croas) ; total number of rears in LDB(LDB-Rear) ; number of fecal boli in LDB (LDB-FB).Subsequently,factor analysis,cluster analysis and correlation analysis were calculated for these parameters.ResultsFactor analysis and cluster analysis of variances from either OFT or LDB revealed a three behavioral dimensions:anxiety factor ( loaded by OFT-Ctime％,OFT-Ccross％ or LDB-Ltime％,LDB-Lcross％,LDB-Lrear％ ),activity factor ( loaded by OFT-Cross,OFT-Rear or LDB-Transition,LDB-Cross,LDB-Rear) and emotionality factor(loaded by OFT-FB or LDB-FB).While all the variances from both OFT and LDB in combination were analyzed,it could be seen as five components:' LDB-anxiety factor ( loaded by LDBLtime％,LDB-Lcross％ and LDB-Lrear％ ),' OFT-anxiety factor ( loaded by OFT-Ctime％ and OFT-Ccross％ ),'LDB-activity factor (loaded by LDB-Transition,LDB-Cross and LDB-Rear),'OFT-LDB-emotionality factor( loaded by OFT-FB and LDB-FB) and 'OFT-activity factor (loaded by OFT-Cross and OFT-Rear).Good correlation were found between OFT/LDB factors alone and in combination,such as OFT-emotionality factor and LDB-emotionality factor(Pearson =0.383,P＜0.05),LDB-anxiety factor and 'LDB-anxiety factor( Pearson =0.989,P＜0.01 ),OFT-anxiety factor and 'OFT-anxiety factor( Pearson =0.934,P ＜ 0.01 ),LDB-activity factor and 'LDB-activity factor ( Pearson =0.956,P ＜ 0.01 ),OFT/LDB-emotionality factor and ' OFT-LDB-emotionality factor ( Pearson =0.835,P＜0.01 ;Pearson =0.696,P＜0.01 ),OFT-activity factor and 'OFF-activity factor( Pearson =0.926,P＜0.01 ).ConclusionEither OFT or LDB comprised three behavioral dimensions:anxiety factor,activity factor and emotionality factor; however,it was difficult to establish face validity as a point-to-point concordance between OFT and LDB.Attention should be paid to heterogeneity of animal models when OFT and LDB were in combination as a behavionomics to evaluate anxiolytics in Kunming mice.

Objective To investigate the effects of RO20-1724 on repetitive ketamine administration-induced learning and memory impairment in immature rats.Methods Forty-eight 21-day-old SD rats of both sexes weighing 45-55 g were randomly divided into 4 groups (n =12 each):control group(group C); ketamine group (group K); ketamine + RO20-1724 group (group K+ R) and ketamine + vehicle (ethanol) group (group K+ A).Ketamine 70 mg/kg was injected intraperitoneally (IP) once a day for 7 consecutive days in groups K,K+ R and K+ A.RO20-1724 0.5 mg/kg and equal volume of ethanol were injected IP at 30 min after IP ketamine once a day for 7 consecutive day in groups K + R and K + A respectively.Morris water maze test was used to assess learning and memory ability.The escape latency and the number of times of passing the safe zone were recorded.The animals were killed after water maze test and their brains removed for microscopic examination of hippocampus and determination of p-CREB protein expression in hippocampus (by Western blot).Results Repetitive ketamine administration significantly prolonged the escape latency,decreased the number of times of passing the safe zone and down-regulated the expression of p-CREB protein in hippocampus on the 3rd and 4th day in group K as compared with group C.RO20-1724 significantly attenuated the above changes induced by repetitive ketamine administration in group K + R as compared with group K.Electron microscopic examination showed that RO20-1724 significantly ameliorated repetitive ketamine administration-induced hippocampal neuronal damage.Conclusion RO20-1724 can ameliorate cognitive dysfunction induced by repetitive ketamine administration.Up-regulation of cAMP /CREB signaling pathway is involved in the mechanism.

876 women with polycystic ovary syndrome (PCOS) were collected from Sep 2004 to Oct 2006. Among them 800 patients with polycystic ovary ultrasound image belonged to group A; the other 76 women without polyeystic ovary ultrasound image belonged to group B. As compared with group A, group B showed significantly higher hirsutism scores, serum testosterone, cholesterol, and lower density lipoprotein. Significantly higher prevalences of diabetes mellitus and hypertension appeared in the first degree relatives in group B than in group A.

Objective To investigate the effect of RO20-1724 on the cognitive function in immature rats after ketamine anesthesia.Methods Ninety-six SD rats of both sexes,aged 21 days,weighing 45-55 kg,were randomly divided into 8 groups ( n =12 each):control group (group C) ; ketamine group (group K) ; ketamine + normal saline group (group K + N) ; ketamine + anhydrous alcohol group (group K + A) ; ketamine + 4 different doses of RO20-1724 groups (group K + R1-4 ).The rats were anesthetized with intraperitoneal injection of kctamine 70 mg/kg in groups K,K+N,K+A and K+.R1-4.Normal saline 2 ml,anhydrous alcohol (in normal saline 2 ml),and RO20-1724 0.25,0.50,0.75 and 1.00 mg/kg (in anhydrous alcohol 8 μl and then in normal saline 2 ml) were injected intraperitoneally in groups K + N,K + A and K + R1-4 respectively 30 min later.Six rats from each group were randomly selected at 24 h after administration and Morris water maze was used to test the ability of learning and memory.Six rats from each group were sacrificed at 48 h after administration and hippocampus and cerebral cortex were removed for determination of the expression of CREB and phospho-CREB (p-CREB) by Western blot.Ressults Compared with group C,the escape latency was significantly prolonged at 2-4 days after administration,the number of animals' swimming across the platform decreased,and the expression of CREB and pCREB in hippocampus and cerebral cortex down-regulated in groups K,K+ N,K+ E,K+ R1 and K+ R2(P ＜0.05 ).Compared with group K,the escape latency was significantly shortened at 2-4 days after administration,the number of animals' swimming across the platform increased,and the expression of CREB and p-CREB in hippocampus and cerebral cortex up-regulated in groups K + R3 and K + R4 ( P ＜ 0.05).Compared with groups K + R1 and K + R2,the escape latency was significantly shortened at 2-4 days after administration,the number of animals' swimming across the platform increased,and the expression of CREB and p-CREB in hippocampus and cerebral cortex up-regulated in groups K+ R3 and K+ P4(P ＜ 0.05).There were no significant differences in the escape latency,the number of animals' swimming across the platform,and the expression of CREB and p-CREB in hippocampus and cerebral cortex between groups K + R1 and K + R2,and between groups K + R3 and K + R4 ( P ＞ 0.05 ).Conclusion RO20-1724 0.75-1.00 mg/kg can improve ketamine-induced cognitive dysfunction by up-regulating CREB and p-CREB expression in hippocampus and cerebral cortex in immature rats.