The robust nuclear-cytoplasmic transport is vital for eukaryotes'life cycle.Not only proteins and RNAs are transferred to their destination by this system, the important cellular activities such as mitosis are also regulated by the transport pathways via adjusting the local concentrations of some critical cargoes.tRNA is one of the most important biomacromolecules in the cell, synthesized in nucleus, and taking part in protein synthesis in cytoplasm.It is a long-held belief that tRNA functions only as a key player in protein synthesis, and tRNA nuclear-cytoplasmic transport is a unidirectional movement from nucleus to cytoplasm.However, the recent discoveries are overturning the traditional opinions.Nuclear-born tRNA can be exported out of nucleus, while cytoplasmic-residing tRNA can also be retrogradely transport into nucleus.The new concept tRNA nuclear-cytoplasmic dynamics was coined in 2008 to describe the tRNA communication between nucleus and cytoplasm, which was found able to regulate protein synthesis and cell cycle in S.cerevisiae.The latest results in this field are going to change the landscape of eukaryotic tRNA biology.

Sixty cases of the patients sick with simple obesity complicated with hyperlipidemia were randomly divided into the magnetic acupuncture group and control group, to observe the improving situation in the indexes of obesity and blood lipid. In the moment the therapeutic effect in reducing body weight, magnetic acupuncture is obviously better in improving cholesterol (TC) in indexes of blood lipid than single acupuncture (P＜0.05).

Objective To evaluate effect of propofol on the first spike latency-amplitude curve of rat inferior colliculus neurons and to find out mechanisms that propofol leds to the disappearance of auditory nerve electrophysiological .Methods 43 healthy specefic pathogen free(SPF) grade sprague dawley(SD) rats(weighing 200-250 g) were used to established wakeful animal models by ventilating with animal respirator after infusing vecuronium .A microelectrode was penetrated in the inferior colliculus (IC) ,and then research first spike latency-amplitude(FSL-A) using a Tucker-Davis Technologies System 3(TDT3) before and after intraper-itoneal injection of propofol 100 mg/kg of each 10 minutes interval .Results CFs ranging from 2 .5 to 44 kHz .An acoustic response of neurons showed offset response ,the remaining 42 neurons showed onset response .r2 of FSL-A curve equations are significant difference between administered propofol before and after 10 minutes(P<0 .05) ,all of them are larger than 0 .95(P<0 .05) .FSL-A curve after administration can shift the lower curve and coincident with the previous administration in the same rat .Conclusion Propofol affect auditory information transmission by convert localization of FSL-A curve of rat inferior colliculus neurons ,but does not change the meaning of the information encoded .

Circulating tumor cells(CTCs)are essential for establishing metastasis and recurrence in non-small cell lung cancer(NSCLC). With the development of detection technique,results from recent studies suggest that CTC level could be a supplement for TNM staging system and a prediction marker of prognosis and therapeutic efficacy,and even could be applied for risk assessment of early NSCLC. Furthermore,as a non-invasiveliquid biopsy,detection of CTC can reflect the molecular biology and genetics characteristics of the primary tumor,and subsequently assist in selecting an optimal individualized treatment.

Face ; surgery ; Hand Transplantation ; Humans ; Organ Transplantation ; Transplantation, Homologous

Allostasis ; physiology ; Chronic Disease ; General Adaptation Syndrome ; physiopathology ; Humans ; Stress, Psychological ; physiopathology

Parathyroid carcinoma is a rare maligant tumor.The main clinical symptoms are primary hyperparathyroidism and all kinds of metabolism disorder and the damage of homologous tissues and organs.The consummate laboratory examination and the accurate imaging localization and the credible histopathologic feature are key points to diagnosis of parathyroid carcinoma.Parathyroid carcinoma is unsensitive to chemotherapy and radiotherapy,and en bloc resection of the carcinoma is the preferred treatment approach.AntiPTH immunization as a new treatment of parathyroid carcinoma has been paid close attention.The prognosis of parathyroid carcinoma is mainly depended on whether the tumor was cut off completely and the control as well as the monitor of the symptomatic hypercalcemian after operation.This article reviews the latest researches and introduces the latest advancement in the diagnosis and treatment of parathyroid carcinoma.

Objective To investigate the effects of propofol on activation of NF-κB in polymorphonuclear neutrophils (PMNs) in rats with LPS-induced acute lung injury (ALI). Methods Sixty healthy SD rats of both sexes, aged 3 months, weighing 250-350 g, were randomly divided into 5 groups (n = 12 each):control group (group C), ALI group and 3 different dose of propofol groups (group P1, P2, P3). The animals were anesthetized with intraperitaneal 3% pentobarbital sodium 40 mg/kg. LPS 5 mg/kg was injected via femoral vein in group ALI.Propofol 5, 10 and 15 mg· kg- 1· h- 1 was infused intravenously over 2 h immeliately after injection of LPS 5 ng/kg through femoral vein in group P1, P2 and P3 respectivey. In group C normal saline 10 ml was injected via femoral vein instead. All rats were killed by exsanguination at the end of infusion of propofol. The right lung was removed for microscopic examination. The morphologic changes were scored 0-3 (0 = normal, 3 = severe morphologic changes). Blood samples were collected from carotid artery for determination of the expression of total NF-κB and activated NF-κB in PMNs by Western blot. Results Compared with group C, morphologic change scores and activated NF-κB expression in PMNs were significantly increased in group ALI, P1 and P2, and morphologic change scores increased in group P3. Morphologic change scores in group P1 and P2 and activated NF-κB expression in PMNs in group P1, P2 and P3 were significantly decreased compared with those in group ALl. Morphologic change scores and activated NF-κB expression in PMNs were decreased gradually in group P1, P2 and P3 . There was no significant difference in total NF-κB expression in PMNs among all groups. Conclusion Propofol can attenuate ALI induced by LPS through inhibition of the activation of NF-κB in PMNs in rats.

Objective To investigate the expression of inducible nitric oxide synthase (iNOS) and p53 protein in hepatocellular carcinoma (HCC) and their relationship with angiogenesis. Methods Immunohistochemical method and image analysis technique were used to detect the expression of iNOS and p53 protein in tumor tissue sections of 59 HCC patients. Microvessel density (MVD) was counted by immunohistochemical staining with anti CD34 antibody.Results ①The expression rates of iNOS and p53 were 81.4%(48/59), 64.4%(38/59) in HCC patients, respectively. The expression intensities of iNOS and p53 were 5 635?1 287, 3 352?873 in HCC patients, respectively. ②MVD was 32.5?2.73 in the tumor tissue of HCC patients. ③The expression of iNOS was correlated with the expression of p53 and MVD in HCC patients ( P

Purpose Glioblastoma and metastasis are the common and aggressive type of brain tumors in adults,and a definitive diagnosis has an important guiding significance to the clinical practice.Our purpose is to investigate the value of post contrast-enhanced T1WI-based histogram analysis in the differential diagnosis of glioblastoma and solitary brain metastasis.Materials and Methods Sixty-eight consecutive patients with glioblastoma or solitary metastasis confirmed pathologically or surgically at Meizhou People's Hospital between January 2012 and November 2015 were included in the study.Glioblastoma was diagnosed in 34 patients and solitary brain metastasis was diagnosed in the rest 34 patients.All patients had undergone routine brain MR examination before surgical resection and the imaging data were retrospectively analyzed.The ROIs were manually placed in enhanced solid parts of the tumors on the maximal cross-sectional post contrasted-enhanced T1WI by Image J.Then the mean,standard deviation (SD),minimum,maximum,skewness and kurtosis were calculated respectively.Results Kurtosis of post contrast-enhanced T1WI of metastasis (1.260 ± 1.271) was statistically higher than that of glioblastoma (0.071 ± 0.667)(P＜0.05).When the optimal threshold criterion ofkurtosis was set at 0.736,the areas under ROC curve of kurtosis was 0.792 (95％ CI:0.676-0.881),and the sensitivity,specificity,positive predictive value and negative predictive value at the optimum cutoff value were 76.47％,88.24％,80.65％,78.38％,respectively.Conclusion Kurtosis of post contrastenhanced T 1 WI-based histogram is valuable in differential diagnosis of glioblastoma and brain metastasis,and thus provides reliable and objective basis for differentiating the two kinds of tumors.