Circulating tumor cells (CTCs)are special kind of tumor cells in the peripheral blood of patient with tumor.Now CTCs detection has been used in the survival time prediction,post-operational recu-rrence detection,individualized treatment and other aspects in the patients with gastric cancer.As the research going,CTCs will provide new help for the comprehensive treatment of gastric cancer.

Objective To study the biocompatibility of human hair keratin artificial tendon(HHKAT) material in vitro. Methods Human embryonic tendon cell (HETC) was incubated with various concentrations (100%,50%,10%) of extracted liquid of HHKAT material for 1,3,5 and 7 days, respectively. Collagen I expression in HETC was detected by immunocytochemistry. The effects of HHKAT material on the proliferation and ultrastructure of HETC were observed. Results Various concentrations of extracted liquid of HHKAT had no effect on the morphologic, collagen I expression and ultrastructure of HETC. HETC could adhere, grow and proliferate on the surface of HHKAT material. Conclusion HHKAT is a medical biomaterial with good biocompatibility in vitro.

Purpose: To study the immunophenotype and the expression of cytotoxic granule proteins of nasal NK/T cell lymphoma and its significance. Methods: 44 cases of nasal NK/T cell lymphoma were studied by the two-step method of DAKO EnVisionTM using a series of antibodies including CD3, CD20, CD43, CD45, CEI45RO, CD56, CD57, CD79?, TIA-1, granzyme-B and perform. Results: All the cases of nasal NK/T cell lymphoma were CD45 positive. 43% of the cases expressed CD3 with positive signal located in the cytoplasm, which was different from peripheral T cell lymphoma. 45% and 52% of the cases were CD43 and CD45RO positive respectively. Cases that reacted to CD56 accounted for 52% of the cases, 43% of which were also positive to CD3. Concerning the reactions to both CD3 and CD56, 10 cases showed CD3 + CD56 + , 13 showed CD3-CD56 + , 9 were CD3 + CD56- and 12 were CD3-CD56-. None of the 44 cases showed positive reaction to CD20, CD79? and CD57. All cases were reactive to TIA-1. 93% and 95% of the cases showed the reactions to granzyme-B and perform. All the controls were negative to TIA-1, granzyme-B and perform. Conclusions: The immunophenotypes of nasal NK/T cell lymphoma showed less consistency. CD56 was not always positive in the cases of this tumor. The different locations of the positive signal to CD3 showed that the cell lineage of this tumor was different from T lymphocytes. The high frequency of the staining by cytotoxic granule proteins, TIA-1, granzyme-B and perform, showed that these cells may have originated from NK cells. The distinctive differences in immunohistochemical staining of cytotoxic granule proteins in nasal NK/T cell lymphoma make their detection very useful and important in diagnosis and differential diagnosis.

Packing the nose after septoplasty is common practice. The use of postoperative packing has been proposed to reduce the dead space between the subperichondrial flaps and minimize postoperative complications such as hemorrhage, septal hematoma, and formation of synechiae. Additionally, postoperative packing is thought to stabilize the remaining cartilaginous septum and minimize persistence or recurrence of septal deviation. Despite these theoretic advantages, evidence to support the use of postoperative packing is lacking. Additionally, nasal packing is not an innocuous procedure. The use of nasal packing actually cause these complications such as postop- erative pain, mucosal injury, bleeding, worsening of breathing due to sleep disorders, and postoperative infections. Routine use of anterior nasal packing after septoplasty should be challenged for not presenting proven benefit. As alternatives to traditional packing, septal suturing, septal stapler and fibrin glue have been used recently. The purpose of this article is to summarize the progress of traditional packing to no packing after septoplasty.
Bandages ; Epistaxis ; Humans ; Nasal Cartilages ; surgery ; Nasal Septum ; abnormalities ; surgery ; Pain, Postoperative ; Postoperative Complications ; Postoperative Period ; Rhinoplasty ; methods ; Surgical Flaps ; Sutures ; Tampons, Surgical ; statistics & numerical data

Endoscopy ; Female ; Humans ; Mesenchymoma ; surgery ; Nose Neoplasms ; surgery ; Young Adult

Corneal endothelial cell(CEC)is the most critical part for the cornea, of which activity can influence the postoperative vision.It is very important for the clinical cornea preservation considering the function and its self-purification of donor cornea.There are a variety of classical methods, which can significantly prolong the saving time of donor cornea with its good quality of CEC.We reviewed the published papers about present preservation methods of cornea, which can give us many suggestions for the clinical cornea preservation.

Aged ; Humans ; Lung Diseases, Fungal ; complications ; Silicosis ; complications ; microbiology ; Tuberculosis, Pulmonary ; complications

Response surface methodology (RSM) was used to optimizing the ultrasonic-assisted extraction technology of pigment from Coreopsis tinctoria. The results showed that the flavonoids were the main constituents of the pigment Based on single factor experiments, a four-factor-level experiment design were developed by box-benhnhen central composite design method with causal factors of ultrasonic temperature, ultrasonic time, ratio of liquid to raw material, the concentrations of ethanol in solvent and the extract absorbance value for the response. The interactive effects of four crucial technological parameters were assessed by response surface methodology (RSM). The optimum ultrasonic-assisted extraction conditions were as follow: ultrasonic temperature was 70 °C, ultrasonic time was 60 min, the concentrations of ethanol in solvent was 72.25% and the ratio of liquid to raw material was 32.05:1 mL . g-1. Under the optimum extraction technology conditions, the absorbance value was 0. 936. The conditidns are suitable for the extraction process regression analysis and parameter optimization.
Coreopsis ; chemistry ; Ethanol ; Flavonoids ; isolation & purification ; Pigments, Biological ; isolation & purification ; Plant Extracts ; isolation & purification ; Plants, Medicinal ; Regression Analysis ; Solvents ; Temperature ; Time Factors ; Ultrasonics

Objective To determin and compare the significance of the expression of chemotactic cytokines in pleural effusions monocyte chemoattractant protein-1(MCP-1)and regulated on activation,normal,T-cell expressed and secreted(RANTES)levels in tuberculous and malignant pleural effusions.Methods The effusion and serum levels of MCP-1 and RANTES were measured in 37 patients with tuberculous pleural effusion and 35 patients with malignant pleural effusion,and selected 20 healthy people acting as control group.Results The effusion and serum levds of MCP-1 and RANTES in tuberculous effusion patients was significantly higher than that in malignant group(P＜0.01);In tuberculous group,the content of MCP-1 in serum was higher than control group,the serum level of RANTES was no statistic significant difference between control group and tuberculous group.The contents of MCP-1 and RANTES were not correlated with pathologic type of lung.cancer.Conclusion MCP-1 and RANTES technique has an important clinical value in diagnosis and differential diagnosis of tuberculosis and malignant pleural effusion.

BACKGROUND: This study aimed to determine the potential protective effect of inducing hypertransfusion to the gastrointestinal tract following a porcine model of cardiac arrest and cardiopulmonary resuscitation (CPR) by evaluating the influence of gastrointestinal ultrastructure, ATPase and serum diamine oxidase. METHODS: Ventricular fibrillation was induced by programmed electrical stimulation in 16 male domestic pigs (n=8/group). Four minutes after ventricular fibril ation, CPR was performed. The pigs that successfully restored spontaneous circulation received intravenous infusion of either norepinephrine to maintain the mean arterial pressure at 130％ of the baseline before ventricular fibril ation or normal saline. Serum diamine oxidase and gastrointestinal ATPase activity were determined, and histopathological examination of the gastrointestinal tract was performed by light and electron microscopy. RESULTS: CPR caused significant injury to the gastrointestinal tract, elevating serum diamine oxidase and causing destruction of intestinal microvillus in control animals. Na+-K+ ATPase and Ca2+ATPase activity in gastric tissue were significantly elevated in animals receiving hypertransfusion treatment compared with the control animals. Hypertransfusion also significantly reduced serum diamine oxidase to below control levels after CPR. Moreover, severe injury sustained by the gastrointestinal tissue was markedly ameliorated under hypertransfusion conditions compared with the control animals. CONCLUSIONS: Gastrointestinal injury and abnormal energy metabolism were strikingly evident following CPR. Hypertransfusion inducing hypertension can improve energy metabolism and ameliorate gastrointestinal mucosal injury, indicating that hypothermia significantly ameliorates gastrointestinal injury sustained following cardiac arrest.