Objective:To study the mechanisms of neutrophil elastase (NE) induced expression of respiratory mucin MUC5AC. Methods:Using gene recombination techniques,four luciferase reporter gene plasmids containing different length of human MUC5AC gene promoter were constructed.Site-directed mutagenesis technique was used to establish mutants of Sp-l and NF-?B site in MUC5AC gene promoter; the relative luciferase activities were detected in the transfected human pulmonary A549 cells. Results:Series of luciferase reporter gene containing different sequences of human MUC5AC promotor were constructed successfully.NE could increase the expression of luciferase reporter gene plasmid containing -1300bp,-689bp and-324bp version of MUC5AC promoter in the transfected A549 cells (P

Adult ; Aggressive Periodontitis ; diagnosis ; diagnostic imaging ; therapy ; Combined Modality Therapy ; Dental Scaling ; Female ; Humans ; Orthodontics, Corrective ; Radiography ; Tooth Loss ; diagnosis ; diagnostic imaging ; therapy

PURPOSE: This study was designed to identify the degree of self-image and social support among Korean-Chinese adolescents and investigate the relationship between these variables. METHOD: A total of 621 Korean-Chinese adolescents in five middle schools in YanBian, China were recruited from March 1st to the 9th, 2005. Data was analysed using descriptive statistics, Pearson correlation coefficient, t-test, and ANOVA with the SPSS 11.5 program. RESULT: In Korean-Chinese adolescents, the total self-image score was statistically different for age, parents' education status, parents' job and living with parents. In the 12 subscales, scoresof emotional tone, impulse control, sexuality, social functioning, vocational attitudes and self-reliance had significant differences between groups regarding gender. The total self-image was in the average range. However, areas of mental health and family function were lower than average and the scale of idealism washigher than average. The adolescents perceived parent's support was higher then friend's support. There was a positive correlation between self-image and social support. CONCLUSION: The findings suggest there is a need to examine self-image and social support of Korean- Chinese adolescents according to their parents' marital status and a need to develop a program to help these broken family's adolescents.
Socioeconomic Factors ; *Social Support ; *Self Concept ; Male ; Korea ; Humans ; Female ; Family ; China/ethnology ; Child ; *Adolescent Psychology ; Adolescent

We describe two cases in which a forceps imprintdeveloped in the AcrySof ReSTOR IOL optic whileinserting these IOLs into the cartridge with straightclamping forceps. In case 1 ,the AcrySof ReSTOR IOL wasexplanted and observed under scanning electronmicroscopy (SEM). The SEM showed that the stepdesign of ReSTOR Multifocal IOL was well maintained. Incase 2, visual acuity, contrast sensitivity and wavefrontmeasurements were performed and no specific changeswere found. Strong evidence does not exist that suggeststhe on-axis forceps imprint can significantly compromisevisual acuity.

Objective To explore the clinical effects of aortic valvuloplasty in treatment of ventricular septal defect(VSD)with moderate or severe aortic valve insufficiency(AI)in children.Methods Thirteen children less than 14 years old underwent open heart surgery as well as aortic valvuloplasty for VSD with moderate or severe AI.There were 9 cases of subpulmonary VSD,perimenbranous VSD in 4 cases.AI was mo-derate in 4 cases,severe in 9 cases.Results There was no hospital death.Post-operative echocardiogram and X-ray revealed that both cardiothoracic ratio and left ventricular end diastolic diameter decreased markedly(P

A new teaching method was developed in the curriculum of Harmful Microorganisms Control Technology.It is characterized by students’ self-learning followed by student’s instruction.Both students and teacher have succeeded in this model after four stages of practice,in which a pleasant learning atmosphere was created in the classroom.An effective interaction between teacher and students was achieved.Students are viewed as main objects in the classroom and they are encouraged to ask questions,to formulate their own ideas,or to find things out for themselves.Thus,students’ abilities including presentation,communication,competition,and cooperation were enhanced.By adapting their role to the new teaching method,teachers have also improved their teaching skill and strategies.

Objective To analyze the surveillance results of soil-borne nematodiasis in Hefei City from 2012 to 2015,so as to provide the evidence formulating the Hefei"Thirteen-five"parasitic disease prevention strategy.Methods The population in monitoring points in Changfeng County,Lujiang City and Chaohu County were investigated for Ascaris lumbricoides,Trichuris trichiura and hookworm infections with Kato-Katz technique every year from 2012 to 2015,and the children aged less than 6 years were checked for Enterobius vermicularis infection by using the cellophane tape method.Results A total of 12 496 people were investigated from 2012 to 2015,the number of infections was 84,and among them,the number of hookworm infection was the most(70 cases)and the number of A.lumbricoides was the least(3 cases).The infection rate in 2014 was higher than that in other years,and there was a statistically significant difference(P<0.008).There was a statistically significant difference among all age groups over 6 years of age in the infection rates(Χ2=27.229,P<0.05),and the infection rate of the age group over 66 years was higher than that of the other age groups.The infection rate in the female was higher than that in the male(Χ2=5.378, P<0.05).The infection rate in the monitoring point in Changfeng County was higher than that in the other monitoring points (P<0.017).Conclusions From 2012 to 2015,the infection rate of soil-borne nematode infections is lower than 5% in Hefei City.The number of hookworm infection is the most.Therefore,during the〝Thirteen five〝period,the key point of prevention of parasitic infections is hookworm infection.

Objective To explore the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) after focal cerebral ischemia-repeffusion injury in rats. Methods Totally 40 Wistar rats were randomly divided into 2 groups: sham-operated group and iscbemia-reperfusion group. A middle cerebral artery occlusion model was constructed in the 25 rats with Longa's method. The expressions of MMP-9 and TIMP-1 were investigated with immunohistochemistry and HE staining at 6, 24, 48, 72 h and 7 d of reperfusion following 2 h ischemia. Results MMP-9 began to be expressed at 6 h reperfusion, and was obviously increased at 24 h and reached the peak level at 48 h, and then, the expression of MMP-9 began to decrease at 72 h to a low level at 7 d. TIMP-1 positive cells began to arise at 6 h reperfusion, peaked at 24 h, decreased at 48 h and remained a low level at 7 d. The expressions of MMP-9 and TIMP-1 were mainly located in vascular endothelial cells, neurons and gitter cells. The expressions of MMP-9 and TIMP-1 were negative in sham-operated group. Conclusion The expressions of MMP-9 and TIMP-1 are induced to increase by focal cerebral ischemia-reperfusion, and vascular endothelial injury may be the main cause to the high expressions of MMP-9 and TLMP-1.

Adhesives ; chemistry ; Benzene ; analysis ; Chromatography, Gas ; Hexanes ; analysis ; Toluene ; analysis ; Trichloroethylene ; analysis

OBJECTIVETo investigate magnetic resonance imaging(MRI) manifestations of perianal abscess and to evaluate MRI diagnosis for perianal abscess.

METHODSFifty cases of perianal abscess between July 2007 and March 2009 were included in this study. MRI was performed using T1 weighted sequence in axial plane, T2 weighted sequence with fat saturation in axial, coronal and/or sagittal plane, and T1 weighted enhanced sequence with fat saturation in axial, coronal and/or sagittal plane. The location, size, shape, signal characteristics, and enhancement patterns of perianal abscess were analyzed.

RESULTSFifty cases with 51 perianal abscesses were identified. The abscess appeared slight hypo- or isointense signal on T1 weighted imaging, obviously hyperintense signal at cavity and isointense signal at wall on T2 weighted with fat saturation imaging, and marked enhancement at wall. The largest diameters ranged between 0.5 cm and 9.0 cm (mean 3.4±1.7 cm ) in abscess, between 0.2 cm and 8.0 cm(mean 2.7±1.7 cm) in cavity. The abscess was round, oblong and crescent in 23, 18 and 7 cases respectively, and was unilocular in 41 cases and multilocular in 10 cases. The abscess located between levator ani and intersphincteric groove in 23 cases, under intersphincteric groove in 3 cases, across levator ani and intersphincteric groove in one case, on levator ani in 7 cases, on intersphincteric groove in 16 cases and above levator ani in one case.

CONCLUSIONMRI is a noninvasive, convenient and highly accurate procedure for diagnosing perianal abscess, which can demonstrate the anatomical relationship between abscess and anal canal.

Abscess ; diagnosis ; pathology ; Adolescent ; Adult ; Aged ; Anus Diseases ; diagnosis ; pathology ; Child ; Female ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Retrospective Studies ; Young Adult