With the increasing frequency of international exchanges and cooperations,students from foreign countries,such as India and Pakistan,have come to our country for their higher degree education.We studied retrospectively and explored the teaching methods of Histology in English,and further provide our thoughts and viewpoints on improving the teaching qualities of foreign students.

Adult ; Drowning ; Humans ; Hydrogen Sulfide ; poisoning ; Male ; Radiography, Thoracic ; X-Ray Film ; Young Adult

Adult ; Aggressive Periodontitis ; diagnosis ; diagnostic imaging ; therapy ; Combined Modality Therapy ; Dental Scaling ; Female ; Humans ; Orthodontics, Corrective ; Radiography ; Tooth Loss ; diagnosis ; diagnostic imaging ; therapy

China ; Education, Medical ; organization & administration ; standards ; Periodontics ; education ; Research Design ; Surveys and Questionnaires

Communicable Diseases ; epidemiology ; Disease Outbreaks ; Humans ; Population Surveillance ; methods

ObjectiveTo study the relationship between expression of a3 chain of collagen Ⅸ (COLIXA3)mRNA in the population exposed to fluorine and fluorosis,in order to reveal the role of COLIXA3 gene in the pathogenesis of endemic fluorosis.MethodsTwelve cases of mild drinking water-born skeletal fluorosis were selected as case groups in Regiment 123 and 128 of Xinjiang Production and Construction Corps Seven Division,6cases of healthy people living in fluorosis areas for more than 10 years as a internal control group and 6 heathly cases living in non-fluorosis areas for more than 10 years as a external control group.The expression of COLIXA3mRNA of peripheral blood lymphocyte of skeletal fluorosis patients and control groups were determined by using SYBR Green Ⅰ chimeric fluorescent method for real-time quantitative PCR.ResultsThe results of the relative expression of COLIXA3 mRNA of case group,internal control group and external control group were 2.16 ± 0.62,1.06 ± 0.09 and 1.05 ± 0.12,respectively.The COLIXA3 expression in case group was significantly higher than that of the internal control group and the external control group (all P ＜ 0.05),while the difference of COLIXA3expression between the internal control group and the external control group was not significantly different (P ＞0.05).ConclusionsFluorine contributes to the expression of COLIXA3 mRNA in peripheral blood lymphocyte,and the expression is up to 2 times higher than that of the control groups,meaning potential biomarkers.

Adult ; Arthritis ; diagnosis ; diagnostic imaging ; Female ; Humans ; Radiography ; Tuberculosis, Lymph Node ; diagnosis ; diagnostic imaging ; Tuberculosis, Osteoarticular ; diagnosis ; diagnostic imaging ; Ultrasonography

According to research results of the lipid-lowering Chinese medicine at home and abroad in recent years, the paper will elaborate on the research status of the antihyperlipidemia effects of chinese medicine from the aspects of its vitro screening model, effective monomer, compounding, single medicine and antihyperlipidemia traditional Chinese medicine patent prescription. Put the ideas of development and use on the antihyperlipidemia effects of chinese medicine effective monomer and components medicine compatibility, emphasize on through new medicine screening cell model to find the antihyperlipidemia effects of chinese medicine monomer and antihyperlipidemia mechanism, breakthrough the single mode to use the accumulate of experience in clinical research as the development of new drugs. Study against the changes of the single-ingredient fixed component, the modify of the effective components combination of different drugs, the component compatibility of the different pathological link and the properties of the effective monomer, accelerate the theoretical innovation about the combination of effective medicine monomer, improve the research levels of the medicine combination from pieces to component, make the action target, link, and mechanism of herbal pharmacology more clear, promote the new Chinese herbal research, the improvement of the clinical efficient and the theory innovation of traditional chinese medicine.
Animals ; Drug Combinations ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; therapeutic use ; Humans ; Hyperlipidemias ; drug therapy ; Hypolipidemic Agents ; chemistry ; isolation & purification ; therapeutic use ; Medicine, Chinese Traditional ; methods ; trends ; Phytotherapy ; methods ; trends ; Plants, Medicinal ; chemistry

The L-ATC hydrolase and L-SCC amidohydrolase which convert L-ATC to L-cysteine in Pseudomonas sp.TS-1138 are purified about 83.9 and 90.3 fold by salting-out method, Sephadex G-75 gel chromatography, DEAE-cellulose 52 ion exchange and Sephadex G-100 gel chromatography, etc. The purified enzyemes are both demonstrated by SDS-PAGE to be a homogeneous protein. Their molecular weight are about 37.5kD and42.8kDa respectively. The optimum reaction temperature are both 35℃, and the optimum pH are 7.0 and 8.0 respectively. The Km of the two enzymes are 0.67 mmol/L and 0.15 mmol/L, and the Vmax are 0.39?10 -3mmol/L?min and 0.42?10 -3mmol/L?min respectively.

The L-cysteine desulfhydrase gene (cd) of Pseudomonas sp.TS1138 was amplified by PCR,and the amplified gene was recombined in the cloning vector pBluescript SKII.The 1.2kb DNA fragment containing cd was sequenced,and its homology with other desulfhydrases was blast; then the cd was cloned into the expression vector pET-21a(+), and afterward expressed by IPTG inducement.The expression protein was purified by Ni-NTA His-Bind Resin.Then the expression protein was identified by the method of activity staining of desulfhydrase, and the characterization of L-cysteine desulfhydrase and the critical role it played in the L-cysteine biosynthetic pathway were discussed.