2.Epstein-Barr virus positive anaplastic-like plasmacytoma: report of a case.
Wen-sheng LI ; Xiao-ge ZHOU ; Jian-lan XIE
Chinese Journal of Pathology 2013;42(3):203-204
ADP-ribosyl Cyclase 1
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metabolism
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Aged
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Epstein-Barr Virus Infections
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Herpesvirus 4, Human
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isolation & purification
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Humans
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Interferon Regulatory Factors
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metabolism
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Ki-67 Antigen
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metabolism
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Male
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Nasal Cavity
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Nose Neoplasms
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metabolism
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pathology
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surgery
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virology
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Plasmacytoma
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metabolism
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pathology
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surgery
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virology
3.One-stage transanal rectosigmoidectomy by laparoscopy for Hirschsprung's disease: report of 10 cases.
Jian-sheng LIANG ; Yu-zhou LI ; Gan YAO
Journal of Southern Medical University 2006;26(5):691-692
Anal Canal
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surgery
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Child
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Child, Preschool
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Colon, Sigmoid
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surgery
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Digestive System Surgical Procedures
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methods
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Female
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Hirschsprung Disease
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surgery
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Humans
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Infant
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Laparoscopy
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Male
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Rectum
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surgery
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Treatment Outcome
4.Study on Molecular Characterization of Microbial Communities in Tibetan kefir Grain
Jian-Zhong ZHOU ; Ming-Sheng DONG ; Han-Hu JIANG ;
Microbiology 1992;0(04):-
The diversity in Tibetan kefir grains and dynamics of the microbial community during the fermentation of Tibetan kefir by PCR-DGGE fingerprinting technique were studied.The results showed that bacterial community of Tibetan kefir grains was more complex than that of yeast.The bacteria communities between different Tibetan kefir grains showed 78%~84% similarity, and yeast 80%~92%.Bands B, E and N of DGGE profiles of the microbial community during fermentation of Tibetan kefir were present throughout the fermentation.Analysis of sequence date showed the majority of the DGGE bands of bacteria community corresponded to LAB, and the most intense band (band E) was completely homology to Lactococcus lactis.
5.Expression and Purification of Receptor Tyrosine Kinase PDGFR?
Jian-Sheng MAO ; Xiang-Shan ZHOU ; Yuan-Xing ZHANG ;
Microbiology 2008;0(10):-
A fusion expression vector pPIC3.5K-PDGFR? was constructed to express recombinant receptor tyrosine kinase PDGFR? and the right Pichia pastoris transformants were screened on his-deficient plates and YPD-G418 plates by turns after electroporation of strain GS115, a high yield strain named M3 was screened. The strain M3 was cultured in a 5 L fermentor and His-GFP-PDGFR? fusion protein was purified by Ni2+ chelating affinity chromatography. One distinct peak was obtained after elution with 250 mmol/L imidazole. Fusion protein was proved to be 90.08 kD by western blotting, and have tyrosine kinase activity by ELISA. Results showed that the receptor tyrosine kinase PDGFR? was successfully expressed in P. pastoris and could be used as a target for small molecule selective inhibitors screening.
6.Effect of Myriocin on Hypertrophy of Glomerular Mesangial Cells and Extracellular Matrix Production Induced by High Glucose
zhao-hua, XIAO ; jian-hua, ZHOU ; heng-sheng, WU
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To observe the changes of extracellular matrix(ECM) production and hypertrophy of glomerular mesangial cells(GMCs) induced by high glucose(HG),and the inhibitory role of myriocin(ISP-1).Methods GMC cultured with normal glucose(5.6 mmol/L D-Glucose,NG),HG(25.2 mmol/L D-Glucose) and HG plus ISP-1(100 mg/L) for different durations(0,24,48,(72 h)).The sizes of GMC were indicated by forward scatter intensity,measured by flow cytometery,and the levels of fibronection(FN),collagen Ⅳ(Col Ⅳ),laminin(LN),precollagen Ⅲ(Pcol Ⅲ) and hyaluronic acid(HA) in the supernatant of cultured GMC were detec-(ted) by ELISA.Results Compared with NG,HG could induce GMC hypertrophy(P
7.Clinical effects of arthroscopic-assisted allograft reconstruction of anterior cruciate ligament injuries
Quan LIU ; Jian-Sheng ZHOU ; Zhen-Hua LIU ; Yu-Zhou XIAO ;
Chinese Journal of Trauma 2003;0(09):-
Objective To determine 2-4 year intermediate-term clinical effects of deep-freezing bone-ACL-bone allograft plus endobutton plate fixation in reconstruction anterior cruciate ligament(ACL) injuries after ACL injury and estimate the feasibility and validity of the technique.Methods We car- ried out a retrospective study on 15 cases with ACL injury of lateral knee joint reconstructed with deep- freezing bone-ACL-bone allograft plus endobutton plate fixation method from September 1999 to October 2002.The average follow up was 36.9 months.The myodynamic recovery,muscular activity,Rom Lach- man test,Pivot shift test,Lysholm score and X-ray of tunnels enlargement at intermediate term were com- pared with normal ones.Results The circumference of thigh was(0.976?0.119)cm shorter than that of the normal side.Extension limitation of less than 3?was found in 12 cases and knee flexion limita- tion for below 5?in 13.Lachman test showed negative results.Lyshohn score was increased from preoper- ative 66.2?4.6 to postoperative 89.4?3.2.X-ray showed no tunnel enlargement.Conclusions Re- construction of ACL with deep-freezing bone-ACL-bone allograft plus endobutton plate fixation under ar- throscopy can attain equal length and anatomic reconstruction of ACL.Deep-freezing bone-ACL-bone is beneficial to functional recovery of ACL.
8.Outcomes of decompressive craniectomy for severe traumatic brain injury in children
Hansong SHENG ; Shangyu XU ; Hui ZHOU ; Jian LIN ; Bo YIN ; Nu ZHANG
Chinese Journal of Trauma 2012;28(3):211-214
ObjectiveTo evaluate the mortality and morbility of children with severe traumatic brain injury (sTBI) following treatment with decompressive craniectomy and further analyze its long-term outcomes.Methods Seventeen children with sTBI undergone decompressive craniectomy between 2004 and 2010 were retrospectively studied.Quality of life of the patients who survived the operation was assessed by using the King' s outcome scale for childhood head injury (KOSCHI).ResultsOf 17 children with sTBI,the mean preoperative Glasgow Coma Scale (GCS) score was 5.27.Five children (29%) died postoperatively,of whom three children were died of cerebral infarction.Twelve children who survived the operation were followed up for average 4.6 years,which showed the mean KOSCHI score of 4.75.Among the 12 survivors,five patients (42%) experienced posttraumatic shunt-dependent hydrocephalus and four (33%) suffered ipsilateral and/or contralateral hygroma.ConclusionsAlthough a high mortality rate is observed in the children with sTBI after decompressive craniectomy,the survived patients have satisfactory outcomes. Posttraumatic hydrocephalus and hygroma are two common complications after decompressive craniectomy for children with sTBI.
9.An epidemiological study of kidney stone in Pudong New Wrea of Shanghai JIANG Ning,
Ning JIANG ; Jing ZHANG ; Guozeng WANG ; Chang SHENG ; Panshi ZHOU ; Jian WANG ; Yuemin WANG ; Jingcun ZHENG
Chinese Journal of Urology 2008;29(10):687-690
Objective To examine the epidemiology of kidney stone in Pudong New Area ofShanghai and analyze its risk factors. Methods 12 565 residents with the age above 16 years weresurveyed. All subjects needed to answer a questionnaire concerning their sex,age,geographic loca tions,occupation,education status and family history of renal calculi,etc. Ultrasound examinationwas used to diagnose the kidney stone. Results The average prevalence of kidney stone was 3.15%(396/12565),4. 05%(247/6096)in the men and 2.30%(149/6469) in the women,respectively (P<0. 05). The prevalence increased significantly with age of men,whereas the prevalence was highest forwomen aged 50-59. The prevalence between city and rural was not significantly different before age60 (2.58% vs 2. 62%,P>0. 05),whereas the prevalence were significantly different after age 60(6.28% vs 3.36%,P<0.05). The prevalence of subjects with a family history of renal calculi washigher than that without family history (32.02% vs 2.06%,P<0. 01). The prevalence in manage ment staff and vehicle drivers were the highest. Conclusions The prevalence of kidney stone in Pud ong New Area of Shanghai was lower than that in south of China. The relative risk increased in sub jects of aged men,women aged 50 59,with family history of renal calculi,management staff and ve hicle drivers. An understanding of the epidemiology particularly the interactions among different fac tors,may help lead to approach that reduce the risk of stone formation.
10.Uses of fresh herbs with antioxidant effect and prospect for population ecology reconstruction in Macau.
Chun-song CHENG ; Liang-yun ZHOU ; Shi-qiang XU ; Jian-sheng LANG ; Zhi-feng ZHANG
China Journal of Chinese Materia Medica 2015;40(3):410-413
The average life expectancy in Macau is ranking the second in the world, the consumption of fresh medicinal plants is a profound culture in Macau. The paper focus on the distribution of the antioxidant herbs, a comprehensive investigation and analysis the amount of the plant resources was carried out. The antioxidant activity of alcohol extracts was determined by using the DPPH method, and six kinds fresh herbs with high antioxidant free radical activity were screened out. Reference to adult daily dose of vitamin C, it is calculated that the daily dose amount of fresh herbs is less than 200 g. For the expected shortage of resources and the ecological status of Macau, we give some suggestions of herbal introduction in population ecology reconstruction.
Antioxidants
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pharmacology
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Ecosystem
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Free Radical Scavengers
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pharmacology
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Macau
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Plant Extracts
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pharmacology