Objective To investigate the expression of uPAR and VEGF-C in hepatocellular carcinoma (HCC) and its effect on the biological behavior of HCC. Methods The expression of uPAR and VEGF-C in liver tissue specimen of 33 patients with HCC was detected by immunohistochemical staining. Results The positive expression of uPAR and VEGF-C in HCC tissues was located in cytoplasma of cancer cells. The positive rates of uPAR and VEGF-C were 69.7 %(23/33) and 78.8 %(26/33) respectively in 33 cases of HCC. The expression rates of uPAR and VEGF-C were significantly higher in HCC with metastasis than in HCC without metastasis(P<0.05). But there were not significant correlation between the expression of uPAR or VEGF-C and tumor size, tumor grade, AFP level, hepatic cirrhosis. There was a close correlation between uPAR and VEGF-C in HCC(r=0.676, P<0.01). Conclusion The expression of uPAR and VEGF-C is correlated with metastasis, uPAR and VEGF-C may be signs of aggressive phenotype for evaluating treatment and prognosis of HCC. Examination of these two proteins may be helpful to .judge biological behavior of HCC.

Mesenchymal stem cells (MSCs) are a group of stem cells derived from mesodermal mesenchyme.They can be recovered from a variety of tissues,including bone marrow,umbilical cord tissue,umbilical cord blood,peripheral blood,and adipose tissue.Under given conditions,MSCs can differentiate into bone,fat,nerve cells,bepatocytes,and many other cells.Thus,MSCs can be used as a new treatment to substitute for organ transplantation.In recent years,the incidence of end-stage liver disease has been increasing and liver disease has become one of the major diseases affecting human health.Donor scarcity and immunological rejection limit the clinical application of liver transplantation.Numerous studies have confirmed the therapeutic effects of MSCs on hepatic fibrosis,cirrhosis,and other liver diseases,which may be related to the differentiation of MSCs into functional hepatocytes.This paper reviews the capacity,regulation,and molecular mechanism of MSC differentiation,and it discusses the therapeutic effects of different sources of stem cells for liver fibrosis.

Objective To study the effects of electromyography-triggered neuromuscular stimulation on upper extremity functional recovery of acute stroke patients. Methods 60 stroke patients who lost wrist extension even in the electromyography (EMG) within 4 weeks were randomly assigned to experimental group and control group. The experimental group received electromyography-triggered neuromuscular stimulation, and the control group received neuromuscular stimulation. They were assessed with EMG, Fugl-Meyer assessment (FMA), Barthel index (BI) before and 4 weeks after treatment. Results The EMG, scores of FMA and BI improved in all the patients after treatment (P=0.000), and improved more in the experimental group (P<0.05). Conclusion Electromyography-triggered neuromuscular stimulation can improve the motor function of hemiplegia, even in the patients without change of EMG.

Objective To understand the epidemiological characteristics of metabolic syndrome (MS ) among adults in Wanzhou District of Chongqing City in order to provide reference for carrying out the prevention and treatment of MS in this re‐gion .Methods The multi‐stage random sampling method was adopted to perform the questionnaire survey ,body measurements and laboratory detection in the adult residents aged 18 years old and over extracted from 26 villages ,towns and urban streets .The MS prevalence rate was calculated and MS component distribution situation was analyzed .Results A total of 2 587 people were effec‐tively surveyed .The MS total prevalence rate was 13 .8% ,which was risen along with the age increase .The prevalence rates of ab‐dominal obesity ,hypertriglyceridemia ,low HDL‐C level ,hypertension and hyperglycemia were 16 .5% ,23 .5% ,23 .3% ,35 .5% and 27 .2% respectively .The proportions of people possessing at least 1 ,2 ,3 ,4 components and 5 components of MS were 71 .6% , 36 .2% ,13 .8% ,3 .9% and 0 .5% respectively .Conclusion The prevalence rate of MS reaches a certain level in this region ,it is necessary to carry out the health education of MS ,change the adverse lifestyle ,control the corresponding factors ,which could reduce the MS risk and promotes its reversion .

Objective To study the effect of Coxsackie virus B3 (CVB3) infection on islet cells in vitro, and to explore the mechanism of islet cells caused by CVB3. Methods Bone marrow mesenchymal stem cells( BMSCs) were separated from the bone marrow and cultured. Then they were induced to differentiate into islet-like cells using nicotinamide and mercaptoethanol. Differentiated cells were detected by morphology , special staining and RT-PCR. Observe CVB3 infection on islet cells under inverse microscope and detect the specific gene fragment by RT-PCR. Results BMSCs showed half suspended shape and gathered to form a cluster after induction. Cells became red brown by dithizone specific staining. RT-PCR also proved the existence of mRNA expressing insulin. Infected islet cells appeared typical pathological changes like shrinks, refraction decreases. RT-PCR detected the desired specific gene fragment of 299 bp in infected islet cells. Conclusion CVB3 can directly injury islet cells, and damage the function of islet cells of secreting insulin.

Evidence-Based Medicine ; Humans ; Occupational Diseases ; prevention & control ; Occupational Medicine ; methods

Carcinoma, Hepatocellular ; Hepatitis B virus ; Humans ; Liver Neoplasms ; MicroRNAs ; Trans-Activators

Alcohol Drinking ; adverse effects ; Cardiovascular Diseases ; etiology ; prevention & control ; Health Promotion ; Humans ; Risk Assessment

Objective: To compare the effects of engine-driven rotar y nickel-titanium(ni-ti) file and stainless steel hand K-file (ss-K-file) in posterior teeth’s root canal preparation. Methods: 118 root c anals of 45 cases were instrumented with ni-ti file (ProFile)by crow-down te chnique and 127 root canals of 49 cases were treated with ss-K-file by step- back technique respectively. The incidence of pain after preparation and the res ults of obturation were observed. Results:Total pain incidence i n ni-ti file preparation group and in ss-K-file group was 20.00% and 46.94% respectively (P

Objective To investigate the effect of TanshinoneⅡA (TSN) on the cell hypertrophy induced by angiotensinⅡ(AngⅡ) in the primary culture of neonatal rat cardiomyocytes. Method The effect of TSN on cardiomyocyte was evaluated by the 3-[4, 5-dimethylthiazol-2-yl] -3, 5-diphenylformazan (MTF) assay. As the index of eardiomyocyte hypertrophy, protein synthesis rate was measured by H-Leucine incorporation and the cell size was determined by phase contrast microscope. The proto-oncogene c-los mRNA and c-jun mRNA expression was assessed using reverse transcription polymerase chain reaction (RT-PCR). Results Exposure of the myocytes to TSN (5～80 mmol/L) for 24hours produced no cytotoxicity. Protein synthesis rate and proto-oneogene c-fos and c-Jun mRNA expression of eardinmyoeytes increased significantly after AngⅡtreatment, and TSN inhibited these effect of AngⅡ.Conclusions TSN can prevent the hypertrophy of eardiomyocytes induced by AngⅡ, which be attributable relate to the decreased expression of proto-oncogene c-los and c-jun mRNA by TSN.