Objective To study the respiratory tract virus infection in dengue fever patients in Guangzhou. Methods Totally 280 patients with dengue fever in 2014 and 32 patients from May to December 2015 were select-ed as group A and group B respectively. The distribution of clinical symptoms and signs ,age and onset time of the 2 groups were compared. Simultaneously ,other respiratory viruses in all patients were detected. Results In group B,the incidence of fever,bone pain,myalgia/body weight fatigue,anorexia,diarrhea,rash diagnosed and hemorrhage was higher than that in group A. The incidence difference of headache,bone pain,myalgia/body weight fatigue,anorexia and rash in 2 groups indicated no statistical significance(P > 0.05)but the incidence of anorexia was significantly different(P<0.05). In group A,there were a larger proportion of patients aged from 21to 30(28.57%)and from 31 to 40(25.36%)while in group B,there were a larger proportion of patients aged from 41 to 50 (19.69%) and from 51 to 65 (25.00%). There was no significant difference in the distribution of other age groups(P > 0.05)except that of 51 ~65 years in 2 groups(P < 0.05). In 2014,the peak inci-dence of dengue fever was in October (64.29%),followed by November (28.21%) and in 2015 ,October was the peak incidence of dengue fever (84.38%),followed by November (12.49%). The incidence in 2 groups in October was significantly different (P < 0.05). In 280 cases of group A ,positive rate of 9 respiratory virus was 30.36%(85/280) and there were 38 cases with MP (44.71%) and 24 with LP1 (27.24%);in 32 cases of group B ,positive rate of 9 respiratory virus was 32.50%(11/32) and there were 4 with MP (36.36%) and 3 LP1(27.27%). The detection rate of other viruses indicated no significant difference(P > 0.05). Conclusions The incidence of dengue fever is different in Guangzhou area at different time. The patients present different clinical symptoms and signs. Analysis of different pathogenic characteristics provides important basis for clinical treatment of dengue fever.

Objective To explore the feasibility and application value of saving the left colic artery (LCA) feasibility in laparoscopic anterior resection of rectal carcinoma (Dixon).Methods Collecting the clinical data of 63 patients diagnosed by rectal cancer and underwent laparoscopic anterior resection of rectal carcinoma from January 2009 to June 2012,including 32 cases underwent saving LCA (saving LCA group) and 31 cases not saving LCA (no saving LCA group).The amount of bleeding volume,operation time,the number of lymph node dissection of inferior mesenteric artery in the operation,pass wind time,anastomotic ischemia,anastomotic leakage,and recurrence and metastasis were compared.Results No significant difference was found in the bleeding volume [(62.82 ± 19.72) ml vs.(60.68 ± 21.39) ml],operation time [(129.48 ± 13.69) min vs.(129.45 ± 19.78) min],the number of lymph node dissection of inferior mesenteric artery in the operation (2.93 ± 1.54 vs.3.21 ± 1.30) between two groups (P ＞0.05).No significant difference was found in pass wind time [(2.82 ± 1.16) d vs.(3.14 ± 0.92) d] after operation (P ＞ 0.05).The saving LCA group was not performed freeing colon splenic area and the terminal ileum stoma,the proximal intestinal blood circulation disorder and anastomotic leakage were not found.In no saving LCA group,4 cases were underwent ileostomy because of the proximal intestinal blood circulation disorder,2 cases occurred anastomotic leakage.During the follow-up of 2-24 months,1 case had a recurrence,3 cases had metastasis in saving LCA group.1 case had a recurrence,4 cases had metastasis in no saving LCA group.Conclusion Saving LCA in Dixon operation can ensure the anastomotic part with adequate blood supply,and reduce the occurrence of anastomotic leakage.

Objective To study the clinical effect of endoscopic thoracic sympathectomy at different segments on palmar hyperhidrosis and research the incidence of postoperative compensatory hyperhidrosis.Methods One hundred palmar hyperhidrosis patients who underwent endoscopic thoracic sympathectomy were divided into 2 groups according to treatment method,the patients in A group (46 cases) received T4 surgery,the patients in B group (54 cases) received T3-4 surgery.The success rate of postoperative,incidence of postoperative compensatory hyperhidrosis and postoperative satisfaction rate between the 2 groups were compared.Results The surgery of 2 groups were successful.There was no statistical difference in total effective rate between the 2 groups (P ＞ 0.05).The incidence of postoperative compensatory hyperhidrosis in A group was significantly lower than that in B group [4.3％ (2/46) vs.20.4％ (11/54)],the postoperative satisfaction rate was significantly higher than that in B group [93.5％(43/46) vs.79.6％ (43/54)],there were statistical differences (P ＜ 0.05).Conclusion Endoscopic thoracic sympathectomy T3-4 or T4 surgery is very effective and safe treatment of palmar hyperhidrosis,but T4 surgery has a lower incidence of postoperative compensatory hyperhidrosis.

Antibodies, Monoclonal ; immunology ; therapeutic use ; Antibodies, Monoclonal, Humanized ; Carcinoma, Squamous Cell ; drug therapy ; Cetuximab ; Head and Neck Neoplasms ; drug therapy ; Humans ; Receptor, Epidermal Growth Factor ; immunology

Objective To understand the effects of NO signal system on the ciliary beating frequency (CBF) of airway epithelial cellMethods Nine normal male Sprague-Dawley rats were anesthetized with isoflurane Their tracheas were rapidly removed using aseptic technique The mucosa of trachea were cut into 1mm2 explants and cultured in DMEM The explants were divided into 5 groups as bellow: L-Arg group, 1-Hydroxy-2-oxo-3-(N-ethyl-2-aminoethyl)-3-ethyl-1-triazene (NOC-12) group, D-Arg group, 8-Br-cGMP group, and phosphate buffered saline (PBS)group Actively beating ciliated cells were observed, and their motion was quantified by measuring CBF using phase-contrast microscopy and videotape analysis Results L-Arg increased CBF from (7 43?0 75)Hz to(8 59?0 93)Hz (P

Objective To understand the modulating mechanisms in ciliary motility by NO signal pathway. Methods L arginine, the preferred substrate of NOS, was employed to act upon the cultured rat ciliated epithelia. After pre incubating with L NMMA, a NOS inhibitor, or ODQ,a sGC inhibitor, or Rp 8 Br cGMPS, an antagonist of PKG respectively, tissues were contacted with L arginine again. Ciliary beating frequency (CBF) was measured by phase contrast microscope and videotape analysis. Results L arginine increased CBF siginficantly. The effect of L arginine on CBF was blocked by L NMMA, ODQ or Rp 8 Br cGMPS.Conclusion L arginine may increase CBF via NO sGC cGMP PKG pathway.

As a common disease in clinical, the treatment of lumbar disc herniation (LDH) focused on local intervertebral disc, such as surgery and other interventional therapy treatment, but postoperative complications and recurrence rate has been a difficult problem in the field of profession. With the development of spine biomechanics and anatomy, researches on lumbar herniation also increased. Researchers discovered that the incidence and prognosis of LDH were inseparable with local muscle and soft tissue. As the deep paraspinal muscles, multifidus muscle plays an important role to make lumbar stability. Its abnormal function could reduce the stable of lumbar spine, and the chronic lumbar disease could also lead to multifidus muscle atrophy.
Animals ; Humans ; Intervertebral Disc Displacement ; physiopathology ; surgery ; Lumbosacral Region ; physiopathology ; surgery ; Paraspinal Muscles ; physiopathology

Lumbar intervertebral disc herniation clinical symptoms and signs are very complicated, lumbocrural pain is the most characteristic clinical symptoms,and show the site (range or area), intensity, nature, evolution, influence factors and adjoint symptoms and so on has certain characteristics and rules. Among them, the dermatomal pain, kinesthetic dysesthesia, dynamic changing pain and lesions segment vertebral side deep tenderness are the most impotent characteristics and rules of pain, therefore, can be regarded as the main basis of clinical diagnosis of the disease, and also the core content of this article.
Back Pain ; diagnosis ; etiology ; pathology ; Humans ; Intervertebral Disc Displacement ; diagnosis ; pathology ; Lumbar Vertebrae ; injuries ; pathology

Objective Tongue shape and motion is one of the important references for tongue diagnosis.However, current tongue image objectification of tongue diagnosis cannot express the characteristics of tongue.Methods In order to increase shape and dynamic information about the tongue, the methods of key frame selection and linear interpolation were used to tongue 3D dynamic visualization reconstruction model on the basis of 3D static visual model.For the convenient use of the tongue 3D dynamic visualization model, this paper put forward a model of 3D dynamic visualization interaction system which was realized based on DirectX.Results The 3D dynamic visualization model not only had a static visual model of texture and depth information, but also could intuitively describe the tongue motion.Conclusions It provides a method for the auxiliary tongue diagnosis of traditional Chinese medicine (TCM).

BACKGROUND:The relationship of fibroblast growth factor receptor 3 (FBGFR3) and malignant tumor has been confirmed. However, domestic and foreign studies on whether FBGFR-3 plays a role in odontogenic tumor with special biological characteristic are few.OBJECTIVE: To observe the expression of FBGFR-3 in odontogenic tumor.DESIGN: A randomized controlled observation.SETTING: Department of Stomatology, Medical College, Medical College of Huzhou Teachers College; Stomatology Hospital Affiliated to Zhejiang University.MATERIALS:This experiment was carried out in the Stomatology Hospital, Medical College Affiliated to Zhejiang University between January 2003 and December 2004.All the samples were from the odontogenic tumor patients admitted to Department of Pathology, Stomatology Hospital Affiliated to Medical College of Zhejiang University between year 1999 and year 2000, including 29 patients with ameloblastoma, 20 keratocyst and 36 primordial cyst. Normal dental crypt or residual dental lamina epithelium tissue served as control. All the samples were embedded by paraffin, sliced into 3 to 5 μm-thickness sections, placed on polylysine-spread slide, and dried for later use.METHODS: The expressions of FBGFR-3 in the normal dental crypt or residual dental lamina epithelium tissue,odontogenic ameloblastoma, keratocyst and primordial cyst were detected by immunohistochemical method.MAIN OUTCOME MEASURES: Expressions of FBGFR-3 in ameloblastoma, keratocyst and primordial cyst.RESULTS: The expression of FBGFR-3 in the ameloblastoma, keratocyst and primordial cyst was positive, presenting 59%, 45% and 8%, respectively, and there were significant differences in expression of FBGFR-3 among these three;FGGFR-3 was not expressed in the normal dental crypt and residual dental lamina epithelium tissue. FBGFR-3-positive cells gathered in the maturation region of tumor cells.CONCLUSION: FBGFR-3 may be related to the pathogenesis of ameloblastoma and keratocyst, and terminal differentiation mechanism.