Objective To evaluate the relationship between parameters of MS and prostate volume.Methods A total of 843 men aged 30 to 49 years were enrolled from August 2008 to July 2011.Prostate volume by ultrasonography,serum prostate specific antigen,digital rectal examination,urinary analysis,and MS-related parameters were investigated.We evaluated the correlations of prostate volume with MS and MS components.Results Prostate volume was largerin the MS group (28.3±5.5 ml) than in the non-MS group (23.2±5.6 ml,P＜0.05).The prostate volumes in subjects with abnormal levels of fasting plasma glucose (FPG) (25.4±5.5 ml),body mass index (BMI) (25.4±5.3 ml) and waist circumference (30.2±4.8 ml) were increased compared with those of subjects with normal parameters (23.6±5.5 ml,22.6±5.5 and 23.5±5.3 ml,respectively,all P＜0.05).The liner regression analysis showed that the FPG,BMI,WC,systolic pressure and triglyceride had a positive correlation with the prostate volume(r=0.216,0.397,0.411,0.170,0.184,respectively; all P＜0.05),but negatively related to HDL-C(r=-0.107,P＜0.05).Conclusions The patients with abnormal FPG,BMI and waist circumference have larger prostate volume.

Adult ; Biopsy ; methods ; Bronchoscopy ; Humans ; Lung ; pathology ; Male ; Middle Aged ; Pneumoconiosis ; diagnosis ; pathology

Objective To observe the clinical effectiveness and prognostic factors of autologous hemopoietie stem cell transplantation on diabetic peripheral angiopathy patients under different degrees of vascular lesions. Methods Thirty-six patients with diabetic were divided into mild vascular stenosis group (group A: 20 lower extremities, vascular stenosis less than 50% ), moderate vascular stenosis group(group B :26 lower extremities, vascular stenosis of 50%-70% ) and severe vascular stenosis group (group C: 25 lower extremities, vascular stenosis greater than 70% ) according to the degree of vascular lesions. All patients were given the stem cell mobilization for 5 days, then peripheral hemopoietic stem cells were gathered on the last day and transplanted to ischemic limb by using monoeyte stem cell suspend liquid with 3 cm × 3 cm intervals.Each index of three groups was regularly observed ( 1th,12th and 18th month respectively after the operation)and comprehensive evaluated. Meanwhile,the transplantation prognostic factors (age, disease duration, post-prandial 2 hours blood glucose, glycated hemoglobin, blood pressure, triglycerides) were analyzed by multiple regression. Results Twelve months after transplantation, the ankle brachial index (ABI) and percutaneous oxygen pressure (TcPO2) of all three groups increased compared with that before the transplantation (P<0.05). In the 18th month after transplantation, the ABI still increased in group A and B (P< 0.05), and no significant changes were found in group C compared with that in the 12th month. Eighteenth month after transplantation, lower extremities of 8 patients (40%) in group A, 8 patients (31%) in group B and 6 patients (24%) in group C were examined to have neovascularizatian. Age, disease duration, blood pressure, post-prandial 2 hours blood glucose, glycated hemoglobin and triglycerides were all the important factors that affected diabetic peripheral angiopathy recurrence after transplantation. Conclusions The long-term effica-cy of stem cell transplantation on diabetic peripheral angiopathy patients at a mild to moderate degree is superior to that with severe vascular lesions. Close monitoring of blood glucose, blood pressure and triglycerides in the operation perinatal stage is required.They are the important prognostic indicators.

Objective To study the clinical efficacy of full thoracoscopic lobectomy and thoracotomy in treat-ment of non -small cell lung cancer.Methods 200 patients with non -small cell lung cancer were divided into group A and group B according to the different surgical methods,with 100 cases in each group.Group A was given the full treatment thoracoscopic lobectomy,group B was received treatment thoracotomy.Pain situation were evaluated in visual analog scale(VAS)method,clinical efficacy and adverse reactions of the two groups were compared.Results The intraoperative blood loss,operative time,drainage,VAS scores and hospital stay of group A were (253.4 ± 1.9)mL,(181.3 ±9.3)min,(174.6 ±4.2)mL,(4.2 ±1.4)points and (7.2 ±0.6)d respectively,which were significantly better than (409.3 ±2.4)mL,(232.2 ±3.2)min,(312.9 ±2.5)mL,(6.3 ±0.9)points and (13.5 ± 0.6)d of group B,the differences were statistically significant(t =8.928,9.028,8.232,9.701,10.906,all P <0.05).The lymph node metastasis number and the number of lymph node dissection of the two groups had no statistically significant differences(t =2.098,3.092,2.983,all P >0.05).The incidence of complications of group A were significantly lower than those of group B,the difference was statistically significant(χ2 =9.033,P =0.002). Conclusion Full thoracoscopic lobectomy in the treatment of non -small cell lung cancer has better effect compared with thoracotomy,its harm to patients is small,and has fewer postoperative complications.

Objective To investigate the therapeutic effect of ipratropium bromide on bronchial spasm of patients with asthma and chronic obstructive pulmonary disease (COPD).Methods 174 patients with asthma and COPD were selected and divided into two groups, 87 cases in the control group treated with budesonide and formoterol fumarate powder for inhalation , 87 cases in the experimental group received ipratropium bromide on the basis of the control group, pulmonary ventilation function, airway resistance and cytokine levels in induced sputum, the clinical effect and incidence of adverse reactions were compared after the treatment.Results The effective rate in the control group(81.61%)was lower than the experimental group (93.11%), with significant difference (P<0.05); compared with the control group, levels of peak expiratory flow (PEF), maximum midexpiratory flow (MMEF), forced expiratory volume in one second to forced vital capacity ratio (FEV1%), forced vital capacity (FVC) were higher in the experimental group after treatment, levels of airway resistance (Raw), Raw% were lower, level of specific airway conductance (Gsp) was higher after treatment, induced sputum levels of vascular endothelial growth factor (VEGF), intercellular adhesion molecular-1 (ICAM-1), interleukin-13 (IL-13), IL-17 were lower after treatment, with significant difference (P<0.05);there was no significant difference in the incidence of adverse reactions between the two groups.Conclusion The clinical effect of ipratropium bromide in the treatment of asthma and COPD was exactly , ipratropium bromide can significantly improve the pulmonary ventilation function in patients, relieve bronchial spasm, reduce airway resistance, inhibit airway inflammation, and the safety is higher.

Objective To evaluate age-related changes of early plasma renal injury markers in rats with sepsis, which can serve as evidence for the early detection of acute kidney injury (AKI). Methods Twenty-one male Sprague Dawley rats were divided into 3 groups based on their age: young group(12 weeks, n=7), adult group (12 months, n=7), and old group (D-galactose-induced 12 months, 500 mg/(kg.d), for 6 weeks, n=7). All rats were received cecal ligation and puncture (CLP) procedure to induce sepsis. Plasma samples of each rat were collected 0.5 h before CLP, and 0.5 h, 4.5 h, 8.5 h after CLP. For each sample, plasma level of kidney injury markers, including plasma creatinine (Cr), blood urea nitrogen (BUN), neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), interleukin-6 (IL-6), Cystatin C (CysC), insulin-like growth factor-binding protein 7 (IGFBP-7), tissue inhibitor of metalloproteinases-2 (TIMP-2) and [TIMP-2]*[IGFBP-7] were quantified. We adopted the criteria for AKI: plasma creatinine elevation =1.5 times of the baseline level. Within-group longitudinal changes and between-group changes were evaluated. Results Compared with samples collected at 0.5 h before CLP, plasma Cr levels were significantly increased at 0.5 h, 4.5 h and 8.5 h after CLP in all three groups (P<0.05), while plasma IL-6 [(121.1±14.9) pg/ml vs. (98.24±13.37) pg/ml, P=0.009] and BUN [(5.43±0.73) mmol/L vs. (5.06±0.82) mmol/L, P=0.041] significantly decreased at 4.5 h after CLP in adult rats, Only the young rats suffered AKI. In between-group comparison, plasma level of IL-6 [(98.24±13.37) pg/ml vs. (116.86±19.75) pg/ml, P=0.046], TIMP-2 [(4042.56±781.68) pg/ml vs. (5662.57±1527.8) pg/ml, P=0.013], [TIMP-2]*[IGFBP-7] [(0.112±0.03) (ng/ml)2/1000 vs. (0.17±0.052) (ng/ml)2/1000, P=0.021] significantly increased at 4.5 h after CLP and BUN [(5.34±0.43) mmol/L vs. (6.29±0.51) mmol/L, P=0.017] at 8.5 h after CLP in the young group, compared with the adult rats. There was no significant difference in the plasma levels of other markers when compared with 0.5 h before CLP and also no significant between-group difference (P>0.05). Conclusion Only the young rats suffered earlier and more serious sepsis-induced AKI. Plasma level of Cr may be useful for early detection of sepsis. Age may not be a risk factor for early AKI in sepsis. Plasma TIMP-2, [TIMP-2]*[IGFBP-7] are potential markers for the detection of early AKI in sepsis in both young and adult animals.

Objective To investigate the indications and efficacy of endovascular stenting for the treatment of thoracic aortic aneurysm.Methods From March 2005 to October 2008,21 patients with Stanford type B aortic dissection,5 cases of pseudoaneurysm,and 2 patient with intramural hematoma complicated with ulcer of the aortic wall were treated by endovascular stenting under general(2 cases) or local(26 cases) anesthesia in our hospital.Medtronic Talent stent was employed in 10 cases,Medtronic Valiant stent was used in 12,and microinvasive straight-type stent was used in 6 patients.Results The operation was completed successfully in all of the 28 cases.Angiography showed that the gaps in the aorta were closed without endoleak.No patient had chest pain or neurological complications.The patients were discharged in(4.5?1.1) days(range 3-7 days).Five patients developed fever after the operation and were cured by indomethacin in one month.One week after the surgery,in 21 cases,CT scan showed enlarged aortic dissection,improved blood supply,thrombus in the false lumen of the thoracic aorta,and a false lumen in the abdominal aorta.In the 5 cases with pseudoaneurysm,CT scan confirmed that the rupture was closed with thrombus being detected in the lumen.The aortic ulcers that were detected in the 2 cases,who had intramural hematoma,were covered by the stents.Of the patients,a follow-up of(21.3?10.2) months(range 1-40 months) was available in 23 patients,who had no complications during the period.Conclusions Endovascular stenting is a simple,safe and effective method for Stanford type B aortic dissection,pseudoaneurysm,and intramural hematoma,resulting in quick recovery,less complications,and short hospital stay.

Objective To evaluate the values of contrast-enhanced ultrasound in diagnosis of cystic renal cell carcino-ma. Methods A total of 73 patients with renal cystic lesions were included in this study. The image features of ultrasound and contrast-enhanced ultrasound examination were analysed. All of patients underwent surgical treatment and had patholog-ical results. The diagnostic values of the ultrasound and contrast-enhanced ultrasound were analyzed by evaluating the im-age features of cystic renal cell carcinoma. Results There were 64 cases of cystic renal cell carcinoma, 9 cases of benign cyst. With ultrasound and color doppler ultrasound,irregular shape, thickness wall, solid ingredients, divisions and more blood flow signals were found in cystic renal cell carcinoma. Renal cyst showed regular shape, few solid component and thin separation and inconspicuous blood flow signals. In contrast-enhanced ultrasound, cystic renal cancer contrast agent appear-ing time was (15.13±4.21)s, and reached the peak time (23.42±5.68)s, fade time was (28.42±4.27)s. The enhanced mode for fast in and fast out was found in 22 cases (34.3%), fast in and slow out in 30 cases (46.8%), slow in and fast out in 2 cases (3.2%), slow in and slow out in 4 cases (6.4%), and synchronously in and out in 6 cases (9.3%). The hyper-enhancement was found in 42 cases (65.6%), the iso-enhancement and hypo-enhancement in 22 cases (34.4%). In renal cyst, There were three cases out of contrast filling. In other 6 cases, the contrast agent appearing time was (16.67±2.73)s, the peak time was (25.83±3.06)s and fade time was (34.17±4.26)s. The enhanced mode for fast in and fast out was found in 1 case (16.7%), fast in and slow out in 1 case (16.7%) and synchronously in and out in 4 cases (66.6%). The hyper-enhancement was found in 2 cases (33.3%), the iso-enhancement and hypo-enhancement in 4 cases (66.7%). The sensitivity, specificity, positive predic-tive value, negative predictive value and accuracy of ultrasound were 85.9%, 66.7%, 94.8%, 40.0%and 83.6%. The sensitiv-ity, specificity, positive predictive value, negative predictive value and accuracy of contrast-enhanced ultrasound were 92.2%, 77.8%, 96.7%, 58.3%and 90.4%. Conclusion Contrast-enhanced ultrasound can be used in benign and malignan-cy identification of renal cystic lesion.

Objective: To investigate the effect of protein transduction domain-oligomerization domain-hemagglutinin (PTD-OD-HA) fusion proteins on tumorigenic ability of chronic myeloid leukemia (CML) BaF3-P210 cells in mice. Methods: The untreated BaF3-P210 cells and BaF3-P210 cells treated with 40 μmol/L PTD-OD-HA for 48 h were injected into BALB/c mice through the tail vein, respectively. The general status and survival time of mice in each group were observed. The number of peripheral white blood cells (WBCs) was counted. The Wright-Giemsa-stained blood and bone marrow smears were examined. The pathological changes of liver, spleen and lung tissues were observed by HE staining. The expression level of bcr/abl protein in bone marrow cells from mice was determined by Western blotting. Results: The incidence rates of CML in mice in the untreated BaF3-P210 cell group and the PTD-ODHA-treated BaF3-P210 cell group were 90% (9/10) and 80% (8/10), respectively; WBCs counts in the two groups were (44.3±4.8)×109/L and (20.6±3.2) ×109/L (P<0.05), respectively; the expression levels of bcr/abl protein in bone marrow cells in the two groups were 5.13±0.46 and 1.32±0.29 (P<0.05), respectively. The average survivals in the untreated BaF3-P210 cell group and the PTD-OD-HA-treated BaF3-P210 cell group were (101.3±6.2) d and (185.4±8.7) d (P<0.05), respectively. Wright-Giemsa staining showed that the infiltration degree of leukemic cells in bone marrow, liver and spleen was lower in the PTD-OD-HA-treated BaF3-P210 cell group than that in the untreated BaF3-P210 cell group. Conclusion: PTD-OD-HA can inhibit the tumorigenic ability of CML with BaF3-P210 cells in BALB/c mice. Copyright© 2011 by TUMOR.

Carcinoma, Non-Small-Cell Lung ; genetics ; pathology ; therapy ; Combined Modality Therapy ; Gene Expression Profiling ; Humans ; Lung Neoplasms ; genetics ; pathology ; therapy ; Lymphatic Metastasis ; Neoplasm Staging ; Oligonucleotide Array Sequence Analysis ; Prognosis