OBJECTIVE:To investigate the demands of medical staff for relevant medication knowledge in order to provide more targeted pharmaceutical service.METHODS:The consultation records in 2006 were collected and reviewed for an analysis in respect of classification of drugs and contents of consultation.RESULTS:Antimicrobials were most frequently consulted(27.87%).The chief contents in the drug consultation included common name and price of drugs,therapeutic regimen,dosage and administration,adverse reactions etc.CONCLUSION:Retrospective analysis of drug consultation revealed that medical staff should have the knowledge about therapeutic regimen and individuation treatment.Clinical pharmacists are expected to improve their rational drug use level by carrying out the corresponding work.

Objective To investigate the effect of fraxetin on primary cardiomyocyte hypertrophy induced by phenylephrine . Methods Primary SD cardiomyocyte hypertrophy was induced by phenylephrine ,and we observed the effects of fraxetin on cardio‐myocyte hypertrophy induced by phenylephrine .Image‐ProPlus 5 measured the area of cardiomyocyte;[3 H ]‐leucine incorporation assay detected the protein synthesis rate of cardiomyocyte;Real‐time PCR measured the Nrf2 and molecular markers (ANP ,BNP) mRNA expression levels of cardiomyyocyte hypertrophy .Results (1)Primary neonate SD Cardiomyocyte hypertrophy model was successfully established by 80 μmol/L phenylephrine for 48 h ,and Nrf2 expression levels significantly increased in cardiomyocyte hypertrophy model;(2)The increase of cardiomyocyte area ,protein synthesis rate and molecular markers expression of cardiomyyo‐cyte hypertrophy were significantly inhibited by fraxetin in a dose‐dependent manner .Conclusion Fraxetin could significantly inhib‐it the cardiomyyocyte hypertrophy induced by PE .

Objective To establish the separation and expansion method of human umbilical cord derived mesenchymal stem cells (hUCMSCs) and explore the influence of 1,25 (OH)2VD3 on hUCMSCs in vitro.Methods UCMSCs were cultured from adherent tissue pieces and detected by immunohistochemistry.Taking the third generation with good growth state UCMSCs make intervene experiment.Set 10-7 mol/L,10-9mol/L and 10-11 mol/L 3 kind of concentration of 1,25(OH)2VD3 as the experimental group,and a control group (DMEM medium).The morphologic change of UCMSCs was observed by inverted microscope.Observe the cell proliferation by the method of MTT.Detect alkaline phosphatase assay and type Ⅰ collagen assay from immunohistochemistry.And the calcium tubercle formation would be examined after 21 days.Results UCMSCs were cultured from adherent tissue pieces and strongly positive for CD44,CD105 and negative for CD34,CD45.consistent with the hUCMSCs characteristics.Under different concentration of 1,25 (OH)2VD3hUCMSCs proliferation were promoted within 7 days but were suppressed beyond 7 days.The high doses group ( 10-7 mol/L group ) obvious inhibitted the stem cell proliferation.Different concentrations of 1,25 (OH)2VD3 and days have interactive effect (P ＜ 0.05).The differences between different groups with ALP and type Ⅰ collagen assay has statistical difference (P ＜ 0.05).The secretion of ALP and type Ⅰ collagen assay of 10-7 mol/Lconcentration group was higher than others.Typical mineralization nedus was found in intervene groups.Conclusion HUCMSCs can be successfully cultured from the adherent tissue pieces.1,25(OH)2VD3 can effectively induce hUCMSCs to differentiate into osteoblasts in vitro.

Objective To investigate the test-retest reliability and inter-rater reliability of the Fugl-Meyer assessment (FMA),Wolf's motor function test (WMFT),the box-block test (BBT) and the nine-hole peg test (NHPT) in assessing the upper limb motor function of stroke patients.Methods Eighteen stroke patients participated in the study.Each patient was assessed twice within 2 hours with the U-FMA,WMFT,the BBT and the NHPT.Two raters recorded each session separately.Test-retest reliability was investigated by comparing the assessment results of different sessions; interrater reliability was identified by analyzing the assessment results of different raters.Results The intraclass correlation coefficients (ICC) for the U-FMA were 0.988 on test-retest reliability and 0.959 on inter-rater reliability.The ICC of WMFT by mean and median time were respectively 0.996 and 0.393 on test-retest reliability and both 1.000 on inter-rater reliability.The ICC of WMFT by score mean was O.989 for test-retest reliability and 0.977 for inter-rater reliability.The ICC for test-retest reliability of the BBT using affected hand counts and the difference in counts between the affected and un-affected hand were respectively 0.975 and 0.982.The ICC of the NHPT using affected hand times and the difference between the affected hand and unaffected hand times were respectively 0.937 and 0.936 for test-retest reliability,and both 1.000 for inter-rater reliability.Conclusion The U-FMA,WMFT,the BBT and the NHPT are all measurements with good test-retest reliability and inter-rater reliability.They are reliable for assessing the upper extremity motor function of stroke patients.

China ; Hearing Loss, Noise-Induced ; prevention & control ; Hearing Tests ; standards ; Humans ; Noise, Occupational ; adverse effects ; legislation & jurisprudence ; prevention & control ; Occupational Diseases ; prevention & control

Language ; Medicine, Chinese Traditional ; Translations

Adult ; Facial Nerve Injuries ; etiology ; Foreign Bodies ; complications ; Humans ; Male ; Neck ; Parotid Gland

Bone Diseases ; Diagnosis, Differential ; Femur Head Necrosis ; Humans ; Medicine, Chinese Traditional ; methods ; Osteoporosis

Objective To investigate the distribution regularity of TCM science basic syndrome for multilple sclerosis (MS}. Methods Through retrospective analysis for general data of case 223 patients and the information of TCM diagnosis with the method of case analysis and modem mathematical statistic, the syndrome types of the case collected were established. Results General fatigue, weariness and no desire to speak, quadriplegia, megrim, acroanesthesia and so on, which reflect the main symptoms of MS, with occurrence frequency of above 60 percent. Five basic symptoms: Syndrome of haemostasis blocking collaterals (18.0%), dark purple tongue is the most important factor; Syndrome of dampness-heat blocking collaterals (17.2%), yellow greasy furred tongue is the important factor; Syndrome of yin-asthenia and obstruction of collateral (14.8%), rapid and thining pulse is the important factor; Syndrome of endogenous wind of liver and obstruction of collateral due to wind-phlegm (14.1%), physical convulsion is the important factor; Syndrome of asthenia of both qi and blood, and channel qi unfavorable (35.9%), feeble pulse is the important factor. Conclusion The location of MS is channels and collaterals. The characteristic of MS is haemostasis, moist heat, deficiency of yin and wind-sputum, deficiency of qi and blood. General fatigue, weariness and no desire to speak, quadriplegia, megrim, acroanesthesia and so on are the main characteristics of MS.

Objective To study the distribution of rare blood groups in Chinese populations.Methods The authors detected Di b-and Wr b-(Diego),Tja-(P),Jk(a-b-)(Kidd),K+ and K 0 (Kell),Mur+(Miltinberger),Ge-(Gerbich) and Ena-(MNS) using monoclonal,polyclonal antibodies and urea with IAT and U type 96 well microplate technology.Results One Di(a+b-)was found out of 645 donors,6 Mur + out of 900 donors,7 K+ out of 10057 donors,2 Jk (a-b-) out of 48400 donors and no Wr b-,K 0,Ge-,Ena-phenotypes were found among 4000 random donors.Conclusion The frequencies of Di b-,Mur+,Jk(a-b-)in Chinese are higher than those in Caucasian and black people.