OBJECTIVE:To investigate the demands of medical staff for relevant medication knowledge in order to provide more targeted pharmaceutical service.METHODS:The consultation records in 2006 were collected and reviewed for an analysis in respect of classification of drugs and contents of consultation.RESULTS:Antimicrobials were most frequently consulted(27.87%).The chief contents in the drug consultation included common name and price of drugs,therapeutic regimen,dosage and administration,adverse reactions etc.CONCLUSION:Retrospective analysis of drug consultation revealed that medical staff should have the knowledge about therapeutic regimen and individuation treatment.Clinical pharmacists are expected to improve their rational drug use level by carrying out the corresponding work.

Objective To investigate the effect of fraxetin on primary cardiomyocyte hypertrophy induced by phenylephrine . Methods Primary SD cardiomyocyte hypertrophy was induced by phenylephrine ,and we observed the effects of fraxetin on cardio‐myocyte hypertrophy induced by phenylephrine .Image‐ProPlus 5 measured the area of cardiomyocyte;[3 H ]‐leucine incorporation assay detected the protein synthesis rate of cardiomyocyte;Real‐time PCR measured the Nrf2 and molecular markers (ANP ,BNP) mRNA expression levels of cardiomyyocyte hypertrophy .Results (1)Primary neonate SD Cardiomyocyte hypertrophy model was successfully established by 80 μmol/L phenylephrine for 48 h ,and Nrf2 expression levels significantly increased in cardiomyocyte hypertrophy model;(2)The increase of cardiomyocyte area ,protein synthesis rate and molecular markers expression of cardiomyyo‐cyte hypertrophy were significantly inhibited by fraxetin in a dose‐dependent manner .Conclusion Fraxetin could significantly inhib‐it the cardiomyyocyte hypertrophy induced by PE .

China ; Hearing Loss, Noise-Induced ; prevention & control ; Hearing Tests ; standards ; Humans ; Noise, Occupational ; adverse effects ; legislation & jurisprudence ; prevention & control ; Occupational Diseases ; prevention & control

Adult ; Facial Nerve Injuries ; etiology ; Foreign Bodies ; complications ; Humans ; Male ; Neck ; Parotid Gland

Language ; Medicine, Chinese Traditional ; Translations

Bone Diseases ; Diagnosis, Differential ; Femur Head Necrosis ; Humans ; Medicine, Chinese Traditional ; methods ; Osteoporosis

Objective To establish the separation and expansion method of human umbilical cord derived mesenchymal stem cells (hUCMSCs) and explore the influence of 1,25 (OH)2VD3 on hUCMSCs in vitro.Methods UCMSCs were cultured from adherent tissue pieces and detected by immunohistochemistry.Taking the third generation with good growth state UCMSCs make intervene experiment.Set 10-7 mol/L,10-9mol/L and 10-11 mol/L 3 kind of concentration of 1,25(OH)2VD3 as the experimental group,and a control group (DMEM medium).The morphologic change of UCMSCs was observed by inverted microscope.Observe the cell proliferation by the method of MTT.Detect alkaline phosphatase assay and type Ⅰ collagen assay from immunohistochemistry.And the calcium tubercle formation would be examined after 21 days.Results UCMSCs were cultured from adherent tissue pieces and strongly positive for CD44,CD105 and negative for CD34,CD45.consistent with the hUCMSCs characteristics.Under different concentration of 1,25 (OH)2VD3hUCMSCs proliferation were promoted within 7 days but were suppressed beyond 7 days.The high doses group ( 10-7 mol/L group ) obvious inhibitted the stem cell proliferation.Different concentrations of 1,25 (OH)2VD3 and days have interactive effect (P ＜ 0.05).The differences between different groups with ALP and type Ⅰ collagen assay has statistical difference (P ＜ 0.05).The secretion of ALP and type Ⅰ collagen assay of 10-7 mol/Lconcentration group was higher than others.Typical mineralization nedus was found in intervene groups.Conclusion HUCMSCs can be successfully cultured from the adherent tissue pieces.1,25(OH)2VD3 can effectively induce hUCMSCs to differentiate into osteoblasts in vitro.

0.05).Conclusion The expression of TM in different TCM syndromes of gastric carcinoma was different.There exists a certain relation in the expression of TM1 and TM2 in different TCM syndromes of gastric cancer.

To investigate the effects of basic fibroblast growth factor (bFGF) on intracellular free Ca 2+ of heating injury, and observe the relationship between mitogen activated protein kinases(MAPKs)signal pathways and Ca 2+ mobilization. Cultured human fibroblasts were heated at 45?C for 10min, then divided into four groups :①basic fibroblast growth factor (10ng/ml) treatment; ②preincubated cells with PD98059 (10?mol/L) for 30 min followed by bFGF (10ng/ml); ③preincubated cells with SB203580 (10?mol/L) for 30min followed by bFGF (10ng/ml); ④preincubated cells with PD98059 (10?mol/L) and SB203580 (10?mol/L) for 30min followed by bFGF (10ng/ml). The cells were incubated with fluorescence Ca 2+ dye fluo 3/AM at 37 C for 30min, then measured by using laser scanning confocal microscope. The results showed fluorescent intensity of fibroblast heating injury was weak, the concentration of intracellular free Ca 2+ increased after stimulation with bFGF treatment. PD98059 and SB203580 could induce calcium oscillation. A rapid decrease of fluorescent intensity was observed after cells were preincubated with PD98059 and SB203580 at the same time. bFGF induced an increase of cytoplasmic and intracellular free Ca 2+ concentrations. It is suggested that MAPKs signaling pathway has a feedback regulation for free Ca 2+ mobilization.

OBJECTIVE:To study the absorptive effect of activated carbon and desorptive effect of organic solvent on endotoxin.METHOD:The effect of different pretreatment modes,time,temperature,amount of activated carbon as well as treatment time on the absorptive effect of activated carbon were analyzed using limulus reagent test.Different organic solvents and treatment time were used to study the desorptive effect of organic solvent on endotoxin.RESULTS:Activated carbon was treated by high temperature baking or adding 0.5 mol?L-1 NaOH represented same effect in removing endotoxin.It showed that optimal time for absorption of endotoxin was 12 min and the amount of activated carbon was at 1.5%.The absorptive effect was similar under condition of room temperature and 37 ℃.The best effect of desorption achieved by using 75% alcohol as desorption reagent after 8 minutes of shaking.CONCLUSION:The activated carbon could be used to absorb endotoxin effectively under suitable conditions and organic solvent could be used to desorb endotoxin from activated carbon.