Objective To observe the clinical nursing effect of doxofylline in the treatment of bronchial asthma and intervention. Methods 98 cases of bronchial asthma patients in our hospital from June 2014 to November 2016 received as the object of nursing, the patients were divided into control group and treatment group, 49 cases in each group, using multiple theophylline treatment, the control group was given routine care, the treatment group on the basis of the comprehensive nursing, the clinical effects of two groups were compared before and after, adverse reactions and treatment of pulmonary function. Results The total effective rate of treatment group was 89.80％, control group total effective rate was 73.47％, the total effective rate of treatment group was significantly better than the control group (P<0.05), the difference was statistically significant; the treatment group adverse reaction rate was 6.12％, the control group adverse reaction rate was 18.37％, incidence of adverse reaction in treatment group was significantly better than the control group (P<0.05), the difference was statistically significant; two groups of patients after treatment of FEV1, FFV1/FVC and PEF indexes were improved, but the treatment group was significantly better than the control group (P<0.05), the difference was statistically significant. Conclusion Doxofylline the treatment of bronchial asthma in the process of the comprehensive nursing has a good effect, and less adverse reactions. It is worthy of clinical application.

At the present,systemic lupus erythematosus is a heterogeneous disease in which diagnosis,especially early diagnosis,is always rather difficult.Because of this,the systems of classification criteria for clinical diagnosis,which include recommendations of the American college of rheumatology and the systemic lupus international collaborating clinics,have been utilized since 1971.However,there is still no single biomarker that is pathognomonic for the disease.Currently,the diagnosis still requiresold-fashioned clinical acumen with the assistance of standardized clinical and laboratory criteria.In the light of the commentary,future research may lead us to define subtypes of lupus from a genomic and proteomic standpoint.

Objective: To explore the anatomy of tibiofibular syndesmosis for the clinic treatment of tib-iofibular syndesmosis injury. Methods: Twelve embalmed adult lower legs were selected. The configurations , structures and plane which ligaments attached to were observed. Results:Tibiofibular syndesmosis consists of ligaments, plica and synostosis. It's tower-shaped. The plane which ligaments attached to has a shape of swallow's tail. Conclusion:Tibiofibular syndesmosis is strong and elastic. The fixation of injuried tibiofibular syndesmosis should be elastic.

The success of total hip replacement relies on detailed preoperative pla nning and reliable localization during operation.Currently,great advances have been made in the research and application of localization s ystem.The traditional preoperative planning is based on plain radiographs of the p elvis and femur as well as the profile outline of each prosthetic component.Now image-gu ided system(IGS)is used to perform virtual implantation.Surgeons used to localize during total hip replacement only wi th eyes or unreliable localization d evices,but now they begin to use imag e-guided system for surgical navigation during operation.[

Objective To study the mechanism of acute lung injury secondary to isolated severe head injury.Methods Twenty-two male SD rats were randomly divided into normal group(NG,n=4),and trauma group(TG,n=18)which were sacrificed respectively on day 1,3,7.The pathomorphological changes were observed by light and electron microscope.The levels of tumor necrosis factor-alpha(TNF-?)and interleukin-8(IL-8)in bronchoalveolar lavage fluid(BALF)were investigated by radioassay(RIA).The level of soluble intercellular adhesion molecule-1(sICAM-1)in BALF was measured by enzyme linked immunosorbent assay(ELISA).Results Capillary congestion,inflammatory cell infiltration and thickening of alveolar septum were detected in TG from day 1 to 7 by light microscope.The histological score of lung on day 3 in TG was significantly higher than that on day 1 and 7.The ultramicroscopic structure of lung showed swelling of vascular endothelial cells,infiltration of polymorphonuclear neutrophil and monocyte,injury of pulmonary epithelial cells in TG from day 1 to 7.The levels of TNF-?,IL-8 and sICAM-1 in BALF in TG were significantly higher than that in NG and the peak values were on day 3.The histologic score in TG was positively correlated with the levels of TNF-?,IL-8 and sICAM-1 in BALF.Conclusion Isolated severe head injury could induce acute lung injury that reached the most severe degree on day 3.The elevation of such proinflammatory cytokines as TNF-?,IL-8 and sICAM-1 in BALF plays an important role in the development of excessive inflammatory response of lung injury secondary to isolated severe head injury.

Objective To construct recombinant baculovirus expression vector containing hepatitis C virus(HCV) truncated core gene and EGFP gene,and to study the antigenicity of the fusion protein expressed in Sf 9 cells.Methods PCR-amplified HCV truncated core gene and EGFP gene were cloned into the transposed vector pFastBac1 to construct a recombinant plasmid pFastCt-EGFP,by which E.coli DH10Bac was transformed to get the recombinant BacmidCt-EGFP.Insect Sf 9 cells were transfected with BacmidCt-EGFP and the expression of fusion protein Ct-EGFP was screened by SDS-PAGE and Western blotting.Results SDS-PAGE and Western blot analysis showed that fusion protein Ct-EGFP was expressed with the molecular mass of 40 kD.ELISA results showed that the fusion protein reacted with 15 of 28(54%) anti-HCV positive sera.Conclusion The fusion protein Ct-EGFP was expressed in insect Sf9 cells and showed partial antigenicity.

Much progress has been made in the diagnosis and treatment of Hodgkin's lymphoma, which has become a highly curable malignancy.However, prolonged survival makes clinicians pay more attention to long-term toxicities of treatment.Consequently, individualized treatment based on disease stage and risk factors is a research hotspot at present.This article reviews recent advances in the individualized treatment of early-stage classical Hodgkin's lymphoma.

BACKGROUND:Studies have shown that neural stem cells isolated from embryonic rat cerebral cortex can proliferate and differentiate into neurons, astrocytes and oligodendrocytes in col agen gels.
OBJECTIVE:To investigate the effect of neural stem cells combined with col agen gel on the apoptosis of nerve cells in the brain of rats after spinal cord injury.
METHODS:Forty-five spinal cord injury rat models were made through spinal cord hemisection and randomly divided into three groups. At 1 week after modeling, rats in the celltransplantation group were injected al ogeneic neural stem cellsuspension into the injured site, rats in the combination group were administered with al ogeneic neural stem cells/col agen gel suspension into the injured site, and rats in the model group received no treatment. RESULTS AND CONCLUSION:From 1 to 8 weeks after injury, the Basso, Beattie and Bresnahan (BBB) locomotion scores in the combination group were significantly higher than those in the other two groups (P<0.05). Hematoxylin-eosin staining showed that at 1 week after transplantation, there were a few necrotic cells and Bcl-2 positive cells, but a large amount of Bax positive cells in the three groups. Then, the number of Bax-and Bcl-2-positive cells was reduced gradual yin the three groups. At 8 weeks after transplantation, the number of Bax-positive cells was significantly higher in the model group than the other two group (P<0.05), but the number of Bcl-2-positive cells were dramatical y lower (P<0.05). Meanwhile, there were no necrotic cells in the three groups. These findings indicate that neural stem celltransplantation combined with col agen gel scaffold can arrest apoptosis of nerve cells in the brain of rats after spinal cord injury, and promote functional recovery after spinal cord injury.

Objective: To observe the therapeutic effect of vertigo treated by pinching the spine. Methods: In the treatment group, 80 cases of vertigo were treated by pinching the spine and An-pressing and Rou-stationary circular pressing. In the control group, 66 cases were treated by An-pressing and Rou-stationary circular pressing. Results: The total effective rate was 95.0% in the treatment group, and 66.7% in control group. The therapeutic effect in treatment group was better than that in control group (P＜0.01). Conclusions: Pinching the spine to treat the vertigo could get rapid and good therapeutic effect, and may avoid the toxic and side effect of drug treatment.

Objective To discuss the application effect of joint remifentanil propofol anesthesia on upper gastrointestinal peristalsis in the painless gastroscopy.Methods 130 patients who taken painless gastroscopy were choosed,and using the random number table method,they were divided into the observation group and control group. Propofol anesthesia was used in the control group,joint remifentanil anesthesia was used in the observation group,and the anesthetic effect were recorded.Results In the observation group group,the excellent rate of anesthesia was 93.85%,the control group was 80.00%,there was statistically significant difference between the two groups (χ2 =5.481,P <0.05).The propofol dosage in the observation group was (1.73 ±0.24)mg/kg,patients awake time was (5.91 ±1.03)min,and departure from the hospital time was (14.58 ±2.79)min,while in the control group,the propofol dosage was (3.06 ±0.57)mg/kg,patients awake time was (9.44 ±1.87)min,and departure from the hospi-tal time was (22.05 ±4.86)min,there were statistically significant difference between the two groups (t =17.337, 13.330,10.747,all P <0.05).24 cases of observation group of patients with mild 36 cases,moderate,severe in 5 cases;23 cases of control group in patients with mild and moderate 27 cases,15 cases were severe,there was statistically significant difference between the two groups (χ2 =5.376,P <0.05).Conclusion Joint remifentanil propofol used in painless gastroscopy of anesthesia is better than only propofol anesthesia,and with little impact on patients with upper gastrointestinal system,it can reduce the dosage of propofol,shorten the patient awake time and time of departure from the hospital,so it is worth popularization and application in clinic.