1.Investigation report on the technical standards of TCM diagnosis, treatment and rehabilitation equipment.
Hua SHEN ; Tangyi LIU ; Huayuan YANG
Chinese Acupuncture & Moxibustion 2016;36(2):217-220
The questionnaire was adopted so as to investigate the attitudes and recognition of the manufacture eneprises of TCM diagnosis, treatment and rehabilitation equipment (DTRE) to the technical standards of, relevant products. It was found that the construction of the industrial standard and the national standard was lagged behind on TCM DTRE. Under the new situation, the enterprises are highly willing to participate in the development of the industrial, national and international standards and have a certain of understanding on the standard development. Nearly 80 % of enterprises believed that it was necessary to set up the relevant mirror organization for the development of industrial, national and international standard of TCM DITRE. In the future, the standard construction of TCM DTRE must face to the new situation. The constant increasing of the enterprises. and scientific research organizations in the standard construction must promote the development of TCM DTRE.
Equipment and Supplies, Hospital
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standards
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Humans
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Medicine, Chinese Traditional
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instrumentation
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standards
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Rehabilitation
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instrumentation
;
standards
2.Unhealthy psycho-behavioral practices for weight control in adolescent students with overweight or obesity
Bian YANG ; Huijing SHI ; Shen LIU
Chinese Journal of General Practitioners 2012;(10):778-780
A questionnaire survey was conducted in 724 students from three junior high schools in Shanghai Jinshan district.Total 642 students completed the questionnaire with a response rate of 88.7%,among them 75 were overweighted or obese.The positive rate of 6 out 8 unhealthy psycho-behavioral practices related to weight control in overweight or obese students was significantly higher than that of students with normal weight (all P < 0.05).Binary logistic backward stepwise regression analysis showed that overweight or obesity was risk factor for the unhealthy psycho-behavior practices related to weight control in adolescent students.
3.A case report of a secondary tonsil follicular dendritic sarcoma after non-Hodgkin's lymphoma.
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(6):428-429
Follicular dendritic sarcoma is a rare and low-grade malignant soft tissue tumors , often occurs in the lymph nodes, we report a case of tonsil follicular dendritic sarcoma which occured after Non-Hodgkin's lymphoma had be cured. The chief complaint was oropharyngeal foreign body sensation with hemoptysis three years, found in the left neck mass increased with more than 4 months. The left side of the pharyngeal wall thickening and disappearance of parapharyngeal space with the surrounding lymph nodes extremely enlarged and integrated was demonstrated by the contrast-enhanced CT of neck. Finally,the pathological diagnosis was tonsil follicular dendritic sarcoma.
Female
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Humans
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Lymph Nodes
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pathology
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Lymphoma, Non-Hodgkin
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therapy
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Neck
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Oropharyngeal Neoplasms
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diagnostic imaging
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pathology
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Palatine Tonsil
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diagnostic imaging
;
pathology
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Radiography
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Sarcoma
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diagnostic imaging
;
pathology
4.Review of Application Technology of Electroacupuncture Instrument and New Idea of Its Development
Hua SHEN ; Tangyi LIU ; Huayuan YANG
Shanghai Journal of Acupuncture and Moxibustion 2016;35(8):1016-1020
Electroacupuncture instruments as Chinese medicine treatment equipment clinically used most extensively play an important role in improving clinical effect of acupuncture treatment. Although the history of electroacupuncture instruments only has more than 60 years, the development of electroacupuncture equipment has experienced a very rich course. Especially in recentyears, the development of electroacupuncture instruments took on a new developing state. It is certain that thedevelopment of electroacupuncture instruments has entered a new era and the future developing speed and quality of electroacupuncture instruments will be significantly raised. By a review of the developing history of electroacupuncture equipmen, this article technically classifies electroacupuncture instruments and deeply analyzes some problems existent in the development of electroacupuncture instruments so as to propose more strict requirements for the future design of electroacupuncture instruments andsatisfy the demand of clinical acupuncture practice.
5.Expression and Characterization of Human/Murine Chimeric Antibodies with Specificity for CD3 Antigen
Zhihua YANG ; Decheng SHEN ; Xiangyang LIU
Chinese Journal of Cancer Biotherapy 1995;0(02):-
Exons encoding the variable regions of the light and heavy chain of the murine McAb HIT3a against CD3 antigen were isolated from HIT3a gene library and inserted into mammalian expression vectors containing the human K and y1 region exons. The chimeric genes were transfected into murine SP2/0 hybridoma cells by Lipofectin. Antibody levels of culture supernatants from transfectomas ranged 21~32 ?g/ml. The chimeric antibodies bound specifically to human T cells and competed effectivelly with the parental anti-CD3 murine McAb for binding to CD3 antigen on human T cells. The ability to promote antibody-dependent cell-mediated cytolysis was significantly enhanced with the chimeric antibodies as compared with anti-CD3 murine McAb. The mitogenic activity of human T cells can be suppressed and enhanced by the anti-CD3 chimeric antibodies. The cytotoxicity to tumor cells and proliferation of human T cells mediated by the anti-CD3 chimeric antibodies were much higher than IL-2 alone. Chimeric HIT3a antibody is a clinically relevant, genetically engineering antibody with potential use in treatment of graft-versus-host disease in tranplantation, autoimmune diseases and some tumors.
6.Application of RNA interference on gene expression of survivin
Bo SHEN ; Guizhi JU ; Yang LIU
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To construct pSUPER-SVV vector and obtain its expression in HeLa cells,in order to interfere the expression of survivin.Methods Oligonucleotide sequences specific for survivin were designed for RNA interference(RNAi),after annealed they were ligated to pSUPER.basic vector.After sequencing,the vector of pSUPER-SVV was transfected into HeLa cells by LipofectAmine.The expression of survivin was detected by RT-PCR and FCM.Results The RNAi expression vector of survivin was constructed successfully,which depressed the expression of survivin efficiently.Survivin was significantly depressed on the level of mRNA in HeLa cells compared with the control groups(P
7.Clinical effect of different operative methods for cervical spondylotic myelopathy
Yanbing LIU ; Yong SHEN ; Dalong YANG
Orthopedic Journal of China 2006;0(19):-
[Objective]To explore the therapeutic effect of three surgical procedures(anterior approach,posterior approach and posterior-anterior approach) for cervical spondylotic myelopathy(CSM). [Methods]A total of 111 patients with cervical spondylotic myelopathy treated with three surgical procedures from June 2002 to December 2007 were retrospectively analyzed.Group A(anterior approach) comprised 54 patients with a mean age of 57.1 years(ranged,44~75 years).Group B(posterior approach,Laminoplasty) comprised 33 patients with a mean age of 61 years(ranged,41~73 years).Group C(posterior-anterior approach) comprised 24 patients with a mean age of 62 years(ranged,48-78 years).All patients were followed-up,neural function and cervical sagittal alignment were recorded.According to JOA score,preoperative and postoperative follow-up scores were compared,improvement rates were calculated,respectively.[Results]Patients were followed up for 3 months to 5 years with an average of 2 years.JOA scores of the three groups at 3,6,12 months and at the last follow-up after operation were compared with those of preoperation(P0.05).Cervical sagittal alignment(D numerical value) had no statistical difference at the postoperative and the final follow-up between group A and group C(P=0.434,P=0.492,P=0.569).There was significant difference between group B and group A/C(P
8.The application of certified reference materials for clinical mass spectrometry
Lingxiao SHEN ; Jinfei MA ; Qingqing YANG ; Pengyun LIU ; Huafen LIU
Chinese Journal of Laboratory Medicine 2021;44(5):437-441
Reference materials are one of the major approaches to achieve measurement accuracy and metrological comparability. Different functions of reference materials should also be distinguished when applied to mass spectrometry as an emerging technology in clinical laboratory. Proper reference materials for validation, calibration and quality control of measurement method can ensure the accuracy and comparability of test results. Based on the problems of reference materials in clinical mass spectrometry, the precautions for the use of reference materials are summarized in the aspects of measurement method validation, calibrator usage and quality control.
10.The effects of Tong Xin-Luo on human umbilical vein endothelial cells impaired by lysophosphatidylcholine
Min YANG ; Jingfang LIU ; Ming SUN ; Huifang SHEN ; Hongyan ZHOU
Journal of Chinese Physician 2012;14(5):607-609
Objective To investigate the effects of Tong Xin-Luo on cultured human umbilical vein endothelial cells (HUVECs) impaired by Lysophosphatidylcholine.Methods The herbage-contained serum of TXL was prepared,HUVECs were cultured in vitro.The study was designated to 4 group:normal control,LPC group,TXL group,and TXL + LPC intervened group.The cell function was determined by cell morphology and MTT colorimetric assay.Results Compaired with normal control group (0.380 ±0.023 ),LPC ( 0.320 ± 0.024 ) could significantly decrease the cells activity,promote cells death ( P <0.05 ).After TXL intervened(0.424 ±0.034),cells activity was significantly increased,cells death was d significantly decreased( P <0.05 ).Conclusions Tong Xin-Luo could protect human umbilical vein endothelial cells function by against the LPC-induced damage.