3.Study on the regulation of HPAT axis molecular network in patients with aging-physiologic shen-deficiency syndrome.
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(9):841-843
Aging
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genetics
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physiology
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Diagnosis, Differential
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Humans
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Hypothalamo-Hypophyseal System
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physiology
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Kidney
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physiology
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Medicine, Chinese Traditional
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Neuroimmunomodulation
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physiology
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Pituitary-Adrenal System
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physiology
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Thymus Gland
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physiology
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Yang Deficiency
;
physiopathology
4.The application of optogenetics in the treatment of retinal degeneration disease
Yumeng SHEN ; Yiqiao XING ; Yin SHEN
Chinese Journal of Ocular Fundus Diseases 2016;32(3):338-342
Optogenetics is a novel technique which combines optics with genetics.Using genetic means,a selected opsin protein is ectopically expressed in target neurons,which are then stimulated by light to moderate the neuronal circuit,as a consequence to regulate the animal's behaviors.Retinal degeneration like retinitis pigmentosa and aged macular degeneration causes visual impairment and eventual blindness.Optogenetics techniques have opened the door to creating artificial photoreceptors in the remaining retinal circuits of retinal degeneration retinas via gene therapy.However,there are still limitations in optogenetics technique,for example,potential risk in virus infection,the choice of target cells and the low visual resolution of the experiment animal.It has been reported that vision was successfully restored to a certain extent in animal model using optogenetics technique.With higher photosensitivity of opsin protein,longer activation kinetics and higher transfection efficiency of virus vector,optogenetics techniques' application in ophthalmology will be improved.
6.Clinical study of the insulin resistance and pancreaticβ-cell function in patients with gestational diabetes
Yin YANG ; Jie SHEN ; Gugen XU
International Journal of Laboratory Medicine 2014;(21):2891-2892
Objective To investigate the insulin resistance and the changes of pancreaticβ-cell function in order to improve the clinical diagnosis and therapy .Methods 99 cases of pregnant women in the late ministers were randomly selected for the study and divided into three groups(normal glucose tolerance group ,impaired glucose tolerance group ,and gestational diabetes group) .The in-sulin resistance and isletβ-cell function of the three groups were evaluated by HOMA model and its related indicators .Results The blood pressure and blood lipid level was not significant different among the three groups(P>0 .05) .Pairwise comparisons of glu-cose infusion rate ,the first phase insulin secretion ,the second phase insulin secretion ,fasting glucose ,fasting insulin ,HOM A-IR , and HOMA-βwere made among the three groups ,and there were difference with statistical significance(P<0 .05) .Conclusion The situation of insulin resistance and the dysfunction of pancreaticβ-cell in trimester pregnant women would become bad associated with the abnormal glucose tolerance .
7.The measurements of multiple slices CT on the narrow pharyngeal cavity in obstructive sleep apneahypopnea syndrome
Jie SHEN ; Ji QI ; Jian-Zhong YIN ;
Chinese Journal of Radiology 2001;0(08):-
Objective To define the characteristic values of the pharyngeal cavity by comparing the values of measures and ratios between the patients with obstructive sheep apnea-hypopnea syndrome(OSAS) and controls.Methods Sixty-eight OSAS patients who were diagnosed using polysomnography,and 56 healthy people were scanned by MSCT(multiple slices computed tomography)in the awake state.Then, the values were measured on the reformated images and were compared between two groups.Results Of the 63 values,49 values were different between the two groups,and among those,4 values were entered into the Discriminant Functions.These were the left-right diameter(LR)[OSAS:LR=(9.4?3.7)mm,controls: LR=(20.1?5.0)mm,t=-13.820,P=0.000)and the cross-sectional area(XSA)[OSAS:XSA= (54?27)mm~2,controls:XSA=(164?77)mm~2,t=-10.944,P=0.000]of RP,the heights of tongue [OSAS:(75.4?8.3)mm,controls:(58.4?9.8)mm,t=10.476,P=0.000],the lengths of uvula/the lengths of airway(OSAS:0.139?0.039,controls:0.154?0.048,t=-1.983,P=0.050).Conclusions Many measures and ratios were different between the two groups,but there were overlaps between them. Measures using the Discriminant Functions can help the diagnosis of OSAS.
8.Study on the characteristics of α-glucosidase inhibitor from Schizandra Chinensis
Linfeng XU ; Zhongming SHEN ; Jianwei YIN
Chinese Journal of Biochemical Pharmaceutics 2001;22(3):127-129
Purpose The aim is to study α-glucosidase inhibitor from Schisandra Chinensis(Turcz.)Baill.Methods The inhibitor was purified by soaking,ultrafiltration,ion-exchange chromatography and Lead Acetate deposition.Results A α-glucosidase inhibitor was obtained.Conclusion The inhibitor was a kind of non-competition inhibitor with large molecular weight of over 5×105D.
9.Alteration and significance of vascular endothelial growth factor in the serum of patients with glomerular diseases
Yan SHEN ; Aiping YIN ; Xueliang FENG
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(02):-
Objective To study the concentration alteration of serum vascular endothelial growth factor (VEGF) in patients with primary and secondary glomerular diseases (GD), the effect of immune inhibitors on the concentration, and analyze the relationship between VEGF concentration and some clinical indexes. Methods Serum VEGF concentration of GD patients was determined by sandwich ABC-ELISA. Results All of the GD patients had higher level of serum VEGF than the control group. Of the 5 groups divided according to the clinical classification, patients of chronic glomerulonephritis(CGN) group, latent glomerulonephritis(LGN) group, Henoch-Schonlein purpura nephritis(HSPN) group and lupus nephritis(LN) group had higher serum VEGF level than the control group, respectively, with no difference among the above groups. The patients without taking immune inhibitors had higher serum VEGF level than the control group,while no difference was observed between the patients taking immune inhibitors and the control group.Patients with glomerulonephritis had a significantly lower serum VEGF level after receiving immunosuppression aggressive therapy than before. The serum VEGF level of nephrotic syndrome in nephrotic phase was higher than in remission stage. In membranous glomerulonephritis serum VEGF correlated positively with 24-hour proteinuria excretion. The serum VEGF level correlated positively with anti-dsDNA Ab titer and the concentration of ? globulins in LN. Conclusion Cytokine VEGF is involved in the pathogenesis of glomerulonephritis, and in the occurrence and progression of proteinuria. Immunosuppression therapy can inhibit the expression of VEGF. In LN patients, the serum VEGF level is related to LN activity.
10.The Application of Low Dose MSCT in Diagnosing the Pharyngeal Disease
Jie SHEN ; Ji QI ; Jianzhong YIN
Journal of Practical Radiology 2001;0(05):-
Objective To study the best parameters of low dose MSCT in examining the pharyngeal disease.Methods 20 volunteers were scanned by MSCT using 10 mm collimation,10 mm interval,120 kV,200 mA,100 mA,50 mA,25 mA parameters,respectively.All of the data was reformed(collimation 2.5 mm,interval 1.3 mm) on ADW 4.0 workstation.The image quality in different dose were scored respectively by 4 experienced radiolagists. Results The best appropriate parameter was 10 mm collimation,10 mm interval,120 kV,50 mA.Conclusion In examining the pharyngeal disease,the application of low dose(120 kV,50 mA) scan mode not only can assure the image quality but can decrease the exposure to patients.