Drugs, Chinese Herbal ; Humans ; Medicine, Chinese Traditional

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Severe Acute Respiratory Syndrome ; drug therapy ; prevention & control

Aging ; genetics ; physiology ; Diagnosis, Differential ; Humans ; Hypothalamo-Hypophyseal System ; physiology ; Kidney ; physiology ; Medicine, Chinese Traditional ; Neuroimmunomodulation ; physiology ; Pituitary-Adrenal System ; physiology ; Thymus Gland ; physiology ; Yang Deficiency ; physiopathology

Optogenetics is a novel technique which combines optics with genetics.Using genetic means,a selected opsin protein is ectopically expressed in target neurons,which are then stimulated by light to moderate the neuronal circuit,as a consequence to regulate the animal's behaviors.Retinal degeneration like retinitis pigmentosa and aged macular degeneration causes visual impairment and eventual blindness.Optogenetics techniques have opened the door to creating artificial photoreceptors in the remaining retinal circuits of retinal degeneration retinas via gene therapy.However,there are still limitations in optogenetics technique,for example,potential risk in virus infection,the choice of target cells and the low visual resolution of the experiment animal.It has been reported that vision was successfully restored to a certain extent in animal model using optogenetics technique.With higher photosensitivity of opsin protein,longer activation kinetics and higher transfection efficiency of virus vector,optogenetics techniques' application in ophthalmology will be improved.

Humans ; Integrative Medicine ; Medicine, Chinese Traditional ; methods ; Retrospective Studies

Objectives To investigate the expression features and roles of the costimulatory molecules and T lymphocyte subsets from patients with chronic nephritis.Methods The expression of the costimulatory molecules CD 28 and CD 137 on PBMC (peripheral blood mononuclear cells) and T lymphocyte subsets from 52 patients with chronic nephritis were studied by immunophenotyping and flow cytometry analysis.Results The T lymphocyte subsets from patients with chronic nephritis showed an obvious imbalance with reversing CD 4/CD 8 ratio,decreasing CD + 4T cells and increasing CD + 8T cells.The expression of the costimulatory molecule CD 28 on T cells was significantly lower compared with normal controls (P

Objective To perform Low dose dynamic MSCT( multi-slice CT) in sleeping obstructive sleep apnea syndrome ( OSAS) patients correcting the imprecise measure values in waking state, and to exactly analyse the location and extension of the dynamic changes about the condition. Methods Sixteen OSAS patients were scanned both in waking and naturally sleeping period ( end phase of inspiration and expiration). Measured at the narrowest part of the retropalatal ( RP) and retroglossal ( RG) and 5 mm under the tip of epiglottis at the epiglottal ( EPG) at the end period of inspiration in sleeping, respectively, and compared the accurate pos(?)on of the narrowest or occlusive level in 3 phases. All patients were also scanned using cine mode at the narrowest level at the end period of inspiration in sleeping to show the pharyngeal cavity changes during sleep. Results The smallest XSA of RP region (Mw = 47. 50 mm2 ,Me =73. 00 mm2 , Mi =2. 00 mm2 ;Zwe =2. 897,Pwe =0. 003 ;Zwi =4. 192,Pwi

Purpose　The aim is to study α-glucosidase inhibitor from Schisandra Chinensis(Turcz.)Baill.Methods　The inhibitor was purified by soaking,ultrafiltration,ion-exchange chromatography and Lead Acetate deposition.Results　A α-glucosidase inhibitor was obtained.Conclusion　The inhibitor was a kind of non-competition inhibitor with large molecular weight of over 5×105D.

OBJECTIVE: To create a rhinogenic model of acute sinusitis in the rabbits. METHOD: Thirty-two New Zealand white rabbits were divided into four groups: A group (nasal cavity-implanted with synthetic sponge), B group (nasal cavity-implanted with synthetic sponge and bacteria inoculation), C group (bacteria inoculation group), D group (control). The animals were examined by the methods of endoscope, histology and bacteriology after two weeks. RESULT: The success rate of model were 60% (A group), 100% (B group) and 0% (C,D group). Both maxillary sinus and ethmoid sinus had developed inflammation. CONCLUSION A rhinogenic model of acute sinusitis can be created by inserting synthetic sponge instilled with pathogenic bacteria into nasal cavity. This is a simple method and has the potential to be valuable to study on FESS.
Acute Disease ; Animals ; Disease Models, Animal ; Inflammation ; Rabbits ; Sinusitis

Objective To investigate the insulin resistance and the changes of pancreaticβ-cell function in order to improve the clinical diagnosis and therapy .Methods 99 cases of pregnant women in the late ministers were randomly selected for the study and divided into three groups(normal glucose tolerance group ,impaired glucose tolerance group ,and gestational diabetes group) .The in-sulin resistance and isletβ-cell function of the three groups were evaluated by HOMA model and its related indicators .Results The blood pressure and blood lipid level was not significant different among the three groups(P>0 .05) .Pairwise comparisons of glu-cose infusion rate ,the first phase insulin secretion ,the second phase insulin secretion ,fasting glucose ,fasting insulin ,HOM A-IR , and HOMA-βwere made among the three groups ,and there were difference with statistical significance(P<0 .05) .Conclusion The situation of insulin resistance and the dysfunction of pancreaticβ-cell in trimester pregnant women would become bad associated with the abnormal glucose tolerance .