Drugs, Chinese Herbal ; Humans ; Medicine, Chinese Traditional

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Severe Acute Respiratory Syndrome ; drug therapy ; prevention & control

Aging ; genetics ; physiology ; Diagnosis, Differential ; Humans ; Hypothalamo-Hypophyseal System ; physiology ; Kidney ; physiology ; Medicine, Chinese Traditional ; Neuroimmunomodulation ; physiology ; Pituitary-Adrenal System ; physiology ; Thymus Gland ; physiology ; Yang Deficiency ; physiopathology

Optogenetics is a novel technique which combines optics with genetics.Using genetic means,a selected opsin protein is ectopically expressed in target neurons,which are then stimulated by light to moderate the neuronal circuit,as a consequence to regulate the animal's behaviors.Retinal degeneration like retinitis pigmentosa and aged macular degeneration causes visual impairment and eventual blindness.Optogenetics techniques have opened the door to creating artificial photoreceptors in the remaining retinal circuits of retinal degeneration retinas via gene therapy.However,there are still limitations in optogenetics technique,for example,potential risk in virus infection,the choice of target cells and the low visual resolution of the experiment animal.It has been reported that vision was successfully restored to a certain extent in animal model using optogenetics technique.With higher photosensitivity of opsin protein,longer activation kinetics and higher transfection efficiency of virus vector,optogenetics techniques' application in ophthalmology will be improved.

Objective To invetigate the effect and safety of sufentanil mixed levobupivacaine on postoperative analgesia in pediatric caudal block anesthesia.Method Sixty pediatric patients (2 ～ 6 years old) who were undergoing elective abdominal surgery,such as repair hernia of high ligation,were randomly divided into three groups with 20 cases each.after intravenous induction,0.25％ levobupivacaine was injected in sacrum tube in group Ⅰ,0.5 μg/ml sufentanil mixed 0.25％ levobupivacaine and 1.0 μg/ml sufentanil mixed 0.25％ levobupivacaine were injected in sacrum tube in group Ⅱ and group Ⅲ respectively.The analgesia effect,the analgesia time,recover time and adverse reaction were observed and recorded 2,4,8,12,16,24 hours after the surgery.Results The analgesia effect in group Ⅱ、Ⅲ were significantly better than the group Ⅰ when 4、8、12 hours after the operation(P ＜0.05),and the analgesia effect in groupⅢ were significantly better than the group Ⅱ when 8 hours after the operation (P ＜ 0.05).There were no significant differences in three groups when 2、16、24 hours after the operation(P ＞0.05),the analgesia time in group Ⅱ、Ⅲ were significantly longer than the group Ⅰ (P ＜ 0.05),and the analgesia time in group Ⅲ were significantly longer than the group Ⅱ (P ＜ 0.05).There were no differences in the recovery time of three groups (P ＞ 0.05).There were no adverse reactions in three groups.Conclusions 0.5 μg/ml and 1.0 μg/ml sufentanil mixed 0.25％ levobupivacaine may be used on postoperative analgesia in pediatric caudal block anesthesia safely and analgesia effect and time were more better and longer than 0.25％ levobupivacaine singly.The analgesia effect in group with 1.0μg/ml sufentanil mixed 0.25％ levobupivacaine was the best in three groups with the fewest side effects.

Humans ; Integrative Medicine ; Medicine, Chinese Traditional ; methods ; Retrospective Studies

Animals ; Apoptosis ; genetics ; Gene Expression Regulation ; Humans ; Longevity ; genetics ; Medicine, Chinese Traditional ; Neoplasms ; genetics ; Rats ; T-Lymphocytes ; cytology

Purpose　The aim is to study α-glucosidase inhibitor from Schisandra Chinensis(Turcz.)Baill.Methods　The inhibitor was purified by soaking,ultrafiltration,ion-exchange chromatography and Lead Acetate deposition.Results　A α-glucosidase inhibitor was obtained.Conclusion　The inhibitor was a kind of non-competition inhibitor with large molecular weight of over 5×105D.

Purpose To explore the role of scanning laser tomography in the assessment of macular hole surgery. Methods Fifteen eyes of 14 patients with macular holes underwent scanning of their affected macular area using the Heidelberg retina tomograph (HRT). The significance of topographic changes postoperatively were determined in eleven eyes which received vitrectomy surgery. The scan field was set at 15? of the retina and the depth was set to 1.5 mm or 2.0 mm. All the measurements were taken for 3 times and the average value of the 3 measurements was used. Results The average hole area was (0.499?0.34) mm 2 and the maximal depth of the hole was (0.284?0.11) mm. Topographic difference analysis of the eleven eyes showed a significant reduction in the height of the retina after vitrectomy. The maximal depth of the hole was (0.063?0.04) mm postoperatively. Conclusion Scanning laser tomography provides an objective evaluation of the anatomic outcome of the macular hole surgery.

Objective To establish an HPLC method to determine simultaneously the content of six water-soluble chemical constituents (danshensu,protocatechuic aldehyde,caffeic acid,rosmarinci acid,salvianolic acid B,and salvianolic acid A) in Xiangdan Injection,and to compare their content discrepancy of Xiangdan Injection from 20 manufacturers.Methods The HPLC method was established.The column was Alltima C18 (250 mm?4.6 mm,5 ?m) at the gradient elution mode with the flow rate of 1.0 mL/min,column temperature was kept at 25 ℃ and the detector was set at 288 nm.Results Six components were separated clearly.The relationship between the concentration and the peak areas of the six compounds was all linear.The precision,stability,repetition,and average recovery were complied with the limit.Significant difference was found in contents of six chemical constituents in Xiangdan Injections from different manufacturers that the variation of salvianolic acid A content was the most distinct and danshensu was the most quietly.Conclusion The method has been successfully used to quanlify the six compounds in Xiangdan Injection,and then,can offer the reference for the quality control of Xiangdan Injections roundly.