Objective To investigate the clinical values of loop electrosurgical excision procedure (LEEP) in diagnosis and treatment of cervical intraepithelial neoplasia(CIN). Methods Fifty-two patients with cervical intraepithelial neoplasia underwent LEEP following cytology, colposcopy and multiple biopsies, were treated by LEEP. The cure rate, the operative time, bleeding volume and patients reaction of LEEP to the cervical intraepithelial neoplasia were investigated during the follow-up. Results The effective rate was 98.07 % in the patients treated by LEEP. The effective rate was 100 % in the patients in grade CIN Ⅰ or CIN Ⅱ by LEEP. No patients in grade CIN Ⅰ and Ⅱ had recurrence during the follow-up 1 year. The mean operative time was 7.8 minutes. The mean bleeding volume was 10 ml. No secondary bleeding and post-operative infection occurred. All these surgical specimens were checked successfully for pathology diagnosis. Conclusion The advantages to use the LEEP to manage cervical intraepithelial neoplasia include its simpleness to handle, short operative time,less bleeding, less vaginal discharge, safety, and high cure rate. LEEP can offer intact sample for pathological diagnosis. LEEP electrotome is a very ideal therapy for the cervical intraepithelial neoplasia, and can block the development of precancerosis to infiltrating carcinoma effectively.

Epigenetic regulation involved in eukaryotic gene expression plays an important role in the progression of colorectal cancer. Colorectal cancer epigenetics is evolved in DNA methylation which is associated with the activation of oncogene and inactivation of tumor suppressor gene. The potential reversibility of DNA methylation offers exciting opportunities for therapy and diagnosis of colorectal carcinoma.

Objective To study the mechanism of toxicity of hexane. Methods SD rats inhaled 15 g/ma n-hexane statically for 8 hours. Results The levels of GSH in whole blood of rats declined significantly (t-test,P＜0. 01). The levels of MDA in serum of rats revealed increasing trend but without statistical significance (t-test,P＞0. 05). The activities of SOD and GSH-Px in liver of rats decreased significantly (t-test,P＜0.01). Conclusion n-Hexane could induce or enhance the reaction of oxygen free radical in organism,and result in damages of lipid peroxidation,which might be one of the mechanisms of the toxicity of alkane.

Despite a combination of all available treatment, the mortality of liver failure is very high,especially in children patients. Artificial liver support methods have been tested for over 50 years. Standard techniques of blood purification like hemodialysis, adsorption, hemo or plasma filtration as well as bioreactorbased approaches using liver cells or tissues have been used. It' s believed that the damaged liver has the ability to return to normal. Artificial liver support systems are expected to be useful for temporary support of liver function. If the liver does not regenerate to normal functions, an artificial liver support system may be useful as a bridge to liver transplantation. In conclusion, artificial liver support method appears to be a reliable therapy for advanced liver diseases and has significantly decreased the mortality of liver failure. Artificial liver support system has been used in children patients as well, but it still needs more researches.

Objective To evaluate retrospectively the efficacy of cistema magna plastic repair in the treatment of Chiari malformation complicated with syringomyelia Methods Fifty-one patients suffered from Chiari malformation complicated with syringomyelia were treated in our department from May 2004 to October 2009. Seventeen patients received cistema magna plastic repairment (the decompression of posterior cranial fossa, duraplastic repair,resection of hernia below cerebellar tonsil and separation of adherence of arachnoid membrane). Results Clinical symptoms disappeared or improved in 15 cases (effective rate of 90%). MRI showed tonsillar herniation disappeared,syringomyelia narrow. MRI showed cistema magna plastic repair, syringomyelia disappeared or significantly thinned and spinal cord subarachnoid space widened. Effective treatment obtained in 12 cases (effective rate 70%). Conclusions The efficiency of Cistern magna plastic repair treating Chiari malformation with syringomyelia is satisfied.

Objective To evaluate the immunoprotective effect of Schistosoma japonicum (Chinese strain) recombinant signaling protein 14-3-3(rSj14-3-3), and to observe the synergism of rSj14-3-3 and rSjGST proteins as candidate vaccine and the effect of ??-T cells activated by Mtb against Schistosoma japonicum. Methods BALB/c mice immunized with rSj14-3-3 and rSjGST purified through SDS-PAGE, electroelution and dialysis were challenged by cercaria infection. Six weeks after challenging infection, the mice were killed and the worm and egg reduction rates were calculated. Results Worm reduction rate was found to be 32.20% in rSj14-3-3+Freund adjuvant group, 31.10% in rSj14-3-3+rSjGST+Freund adjuvant group, 27.96% in rSj14-3-3+Mtb group, 26.00% in rSj14-3-3+rSjGST+Mtb group, and 27.10 % in rSjGST+Mtb group, respectively, number of eggs in liver tissue was reduced by 50.40%, 53.30%, 51.10%, 58.60% and 51.30%, respectively. Conclusion rSj14-3-3 could induce partial immunity against Schistosoma japonicum in BALB/c mice, and might serve as a candidate vaccine; ??-T cell activated by Mtb played a role in anti-Schistosoma japonicum similar to the immune reactions induced by Freund adjuvant, but no synergistic effect combined with rSjGST was observed.

Objective To study the localization of the signaling protein 14-3-3 of Schistosoma japonicum (Sj14-3-3)in the parasite. Methods Cercariae were collected from the infected Oncomelania hupensis for the infection of rabbits. Fifteen-day-old schistosomula and adult worms obtained from infected rabbits 15 and 42 days post-infection were used for frozen sections and indirect immunofluorescence staining with monoclonal antibody to rSj14-3-3. Results The results showed that the Sj14-3-3 distributed mainly in the tegument, subtegument, muscle, and parenchyma of both adult worms and 15-day-old schistosomula. Conclusion The wide distribution and large sites of Sj14-3-3 in the parasite were clearly demonstrated, which established a significant clue for further studies of biologic actions and application of 14-3-3 protein.

OBJECTIVE To evaluate the effect of air disinfection methods to improve air quality in dental clinic.METHODS Electrostatic attraction method was used to disinfect air in dental clinic.Air samples here collected before,during and after daily and treatments compared.Bacterial colonies were counted. The air effect of disinfection was compared with undisinfected control group in terms of total number of germs.RESULTS Bacterial count of disinfected group was lower than that of undisinfected group(P

BACKGROUND: Basic fibroblast growth factor (BFGF) widely in human tissue participates in trauma repair of various tissues and is one of wound healing factors in the body. OBJECTIVE: To summarize the progress of BFGF in the fracture healing. RETRIEVAL STRATEGY: A computer-based online search of Pubmed Database was undertaken to identify the relevant articles on BFGF in the fracture healing published from January 2000 to December 2007 with the Keywords of "fibroblast growth factor 2 (basic fibroblast growth factor), fracture healing, regulation, signal transduction" in English. At the same time, Chinese relevant articles were searched in China Journal Full-text Database (CJFD) and Wanfang Database published between January 2000 and December 2007 with the same key words in Chinese. Exclusive criteria included articles with repetitive research and non-original study. LITERATURE EVALUATION: A total of 33 articles were collected about the distribution (3 articles), biological characteristics (3 articles) and the effect on the healing of bone fracture (27 articles) of BFGF. DATA SYNTHESIS: Bone healing is the process of regeneration after bone injury and the process of bone formation. BFGF can promote the cell migration by its chemotaxis and accelerate the healing by aggregating mesenchymal stem cells, macrophage and fibroblasts towards trauma region. Present animal studies on BFGF are deep and reports on clinical application are a few. Thus, the effect of BFGF is available. BGFG can affect bone formation by adjusting cell prolifeation and differentiation and changing the synthesis of cell products. BGFG not only can promote the bone growth, but also enhance the adhesion of osteoblasts and scaffold. CONCLUSION: The distribution and biological characteristics of BGFG have been recognized primarily. The further study on modulatory mechanism in the fracture healing of BFGF is carrying through. The bone restoration compounded by BFGF and its carriers is in the experiment and needs to be studied further.

Insulin resistance ,a key component of type 2 diabetes,is recognized as the pathological basis of the metabolic syndrome. H owever,its underlying mechanisms are still not fully understood.Recently,eleva ted levels of free fatty acids (FFA)have been observed in many insulin-resist ance states,which has gradually drawn attention.Not only do FFA interfere in se veral steps involved in glucose metabolism,but also play an important role in i nsulin signal transduction,resulting in decreased insulin-stimulated glucose t ransport. The thiazolidinediones,as insulin-sensitizing drugs,have entered th e market. A prominent effect of these agents is the lowering of circulating FFA levels and it is believed that targeting FFA metabolism may therefore offer wide r expectations in therapy for insulin resistance and type 2 diabetes.