Objective To investigate the effects of Xinnaoxuemianning combined with statins on carotid atherosclerotic plaque in patients with cerebral infarction. Methods 180 cerebral infarction patients were divided into the observation group (90cases) and the control group (90cases) by random number table method. The control group was given atorvastatin calcium tablets and the observation group was given additionally Xinnaoxuemianning. The changes and prognosis of carotid atherosclerotic plaques were evaluated before and after treatment, and the incidence of adverse reactions was counted. Results There were no differences in the carotid atherosclerotic plaque, TCM syndrome score and neurological deficit severity between the two groups before treatment. After treatment, the above indexes were significantly improved, and the increasing degree of the observation group was better than that of the control group. The incidence rate of adverse reactions was lower in the observation group than that in the control group (5.56％ vs.13.33％) (P<0.05). Conclusion Xinnaoxuemianning can reduce the carotid atherosclerotic plaque area and correct the unstable plaques in patients with cerebral infarction.

Objective To establish a HPLC method for determining and comparing the contents of diosbulbin B in
Dioscorea bulbifera L. from different regions. Methods The medicine powders were extracted twice by water, then the water extracts were combined and detected by HPLC at 35℃,with Kromasil-C18 column (4. 6 mm×250 mm,5μm) and a mobile phase of acetonitrile-water-glacial-acetic acid (34660. 1). The flow rate was 1. 0 mL·min-1 and the detection wavelength was 210 nm. Results The linear range of diosbulbin B was 26. 0-260. 0 μg·mL-1(r=0. 999 9). The average content of diosbulbin B in Dioscorea bulbifera L. from different areas was that of Hebei>Guangxi>Jiangsu>Sichuan>Zhejiang. Conclusion The method is simple,quick,accurate and suitable for the determination of diosbulbin B in Dioscorea bulbifera L. from different regions.

Animals ; Antineoplastic Agents ; pharmacology ; therapeutic use ; Brain Neoplasms ; drug therapy ; secondary ; Breast Neoplasms ; drug therapy ; metabolism ; pathology ; Cell Proliferation ; drug effects ; Humans ; Quinazolines ; pharmacology ; therapeutic use ; Receptor, Epidermal Growth Factor ; metabolism ; Receptor, ErbB-2 ; metabolism

Objective To study cerebral cortical activation preoperatively and postoperatively in patients with cervical spondylotic myelopathy (CSM), and discuss effect of the surgery on cortical reorganization in functional recovery.Methods 19 cases with CSM intrial group underwent cervical vertebra canal decompression surgery according to clinical routine.Cases in trial group completed clinical assessment using the modified Japanese Orthopaedic Association Scores (mJOA) prior to decompression and 12 months following surgery, and underwent cerebral functional MRI and conventional MRI in the same time.19 controls also carried out cerebral functional MRI and conventional MRI.All subjects performed a finger-tapping paradigm with right hand during processing functional MRI.The imagings and data of trial group were divided into preoperative group and postoperative group in accordance with the time of completed and than analysed.Results The mJOA score of postoperative group was increased significantly (P<0.001).Cortical volume of activation (VOA) of preoperative group was significantly higher than the control group (P<0.05).VOA was lower in the postoperative group, but still significantly higher than the control group (P<0.05).The preoperative group was able to detect and activate the signal only in the left precentral gyrus.The postoperative group was able to detect and activate the signal in the l left postcentral gyrus, the premotor area and the supplementary motor area, and the right cerebral cortex could also detect a small amount of activation signal.Conclusion CSM patients undergo cerebral cortical remodeling, causing sensory and motor function activation areas to expand and shift.The changes of cortical reorganization after cervical vertebra canal decompression surgery are associated with functional recovery.The surgical treatment may promote the compensating cortical reorganization.

Carcinoembryonic Antigen ; metabolism ; Carcinoma in Situ ; immunology ; pathology ; virology ; Carcinoma, Ductal, Breast ; immunology ; pathology ; virology ; Cervical Intraepithelial Neoplasia ; immunology ; pathology ; virology ; DNA, Viral ; analysis ; Diagnosis, Differential ; Female ; Human papillomavirus 16 ; genetics ; isolation & purification ; Humans ; Ki-67 Antigen ; metabolism ; Uterine Cervical Dysplasia ; immunology ; pathology ; virology ; Uterine Cervical Neoplasms ; immunology ; pathology ; virology

BACKGROUND:No particularly effective medicine could treat human immunodeficiency virus 1(HIV 1) associated dementia(HAD) at present,which mainly because the mechanism of HIV 1 infection induced neural damage and necrosis is still not completely clarified. OBJECTIVE:To investigate the effects of human immunodeficiency virus I type(HIV 1) enveloped protein(EP) gp120 on the synaptic transmission and plasticity in hippocampal CA1 area of rat to clarify the mechanism of HAD generation. DESIGN:A paired design. SETTING:Department of Pathophysiology of Medical College of Jinan University. MATERIALS:The study was conducted in the Department of Pathophysiology(a tertiary national key laboratory of National Administration for Traditional Chinese Medicine,registration number: TCM 03 131) of the Medical College of Jinan University from January to October of 2003.Male SD rats aged between 2 and 5 weeks were used in the study. INTERVENTIONS:Brain slice perfusion and recording technique was employed. MAIN OUTCOME MEASURES:To record the excitatory postsynaptic potential(EPSP) in hippocampal CA1 area in rat;to investigate the effects of gp120 on long term potentiation(LTP) induced by high frequency electric stimulation in Schaffer lateral branch. RESULTS:gp120 had inhibitive effect on LTP in hippocampal CA1 area in rat [the average amplitude of LTP decreased from normal (216.1± 14.0) % to(90.8± 6.0) % ,n=12,P< 0.01],but had no effect on basic EPSP.PKA/PKC protein kinase(PK) inhibitor H7 could reverse this inhibitive effect [the average amplitude of LTP was(198.8± 16.2) % ,n=8,P< 0.01]. CONCLUSION:gp120 might participate the generation of HAD through inhibiting LTP in hippocampal CA1 area.

Objective To compare the immune protective effects of three antigens of Trichinella spiralis encapsulated larvae on mice.Methods The mice were immunized with Trichinella spiralis encapsulated larvae somatic antigen,encapsulated larva excretory-secretory antigen and encapsulated larva surface antigen,3 times with a 7-day interval,and the adjuvant control and normal control group were set up.Seven days after the final immunization,each mouse was orally challenged with 200 Trichinella spirais larvae.The intestinal adult worms and muscle larvae of Trichinella spiralis of each group were recoveried and examined on Day 7 and Day 30 post-challenge,respectively.The level of 8eruln IgG to antigens of Trichinella muscle muscle larvae wa8 detected by ELISA.Results The intestinal adult worms were reduced by 84.89%.89.73%,85.65%.2.57% in the encapsulated larva somatic,excretory-secretory and surface antigen groups,respectively.The muscle lalwae were reduced by 71.71%,80.98%,73.66%,5.60%, respectively.Adtlltwornl reduction rates(P<0.05) and musclelarva reduction rates(P<0.01) of the encapsulated larva excretory-secretory antigen group and surface antigen group were higher than those of encapsulated larva somatic antigen group.The antibody titers in all the immunized groups increased significantly.and the GMRT values of the encapsulated larva somatic,excretory-secretory and surface antigen groups were 32 798.89,3 474.51,2 984.83,respectively,and were 6.09,7.56,6.50 times higher than those of the normal control group(459.32).Conclusions Trichinella spiralis encapsulated larva antigens can induce strong resistance of host to a subsequent challenge infection.Among these antigens,excretory-secretory antigen is more immunogenic.

The data of 173 cases with acute mastitis were collected and reviewed from January 2010 to October 2012.The liquor puris samples were collected for bacterial culture.And drug sensitive test was performed by Kirby-Bauer method.98 samples were cultured with a positive rate of 56.7％.A total of 98 bacterial strains were isolated,Gram-positive bacteria were 91 strains (92.9％) and Gram-negative 7 strains (7.1％).The most common bacteriun Staphylococcus aureus,accounted for 83.7％ was highly resistant to penicillin and erythromycin with resistant rates of 90.2％ and 86.6％ respectively.And there was no resistance to vancomycin and teicoplanin.

Objective To understand the current status of health knowledge demands and its associated factors in general population. Methods A questionnaire-based survey by convenience sampling method was carried out in 700 subjects aged over 15 years old in October 2014. Information of demographics and health knowledge demands were collected. Data was analyzed using SPSS 17.0. Results A total of 653 subjects were responded, with a response rate of 93.3%. Respondents in male and female were 339 (51.9%) and 314 (48.1%) respectively. There were no differences among age , educational levels and self-reported health status between the two genders 86.6% of the participants showed that they had interests in health knowledge. Educational level and age were the factors associated with the interests. 65.8% and 72.3% of people want to get practical health knowledge of western and traditional Chinese medicine respectively. The top concern of health issues were cardiovascular and cerebrovascular diseases. For preferred ways of learning health knowledge , television is the favorite one (44%), especially in elderly (over 63.8%). A relative high option rate was newspaper and magazine in all age groups. However, young people showed that they preferred the ways of the internet and Wechat (about 50%). For health disseminators, the participants in all age had greatest trust in experts in large hospitals (over 60%). The second option was retired old experts. The experienced patients were convinced in some degree and increased with age. Conclusions In future, health education in general population should considered the characteristics of the population and the applicability of knowledge. Besides , it is necessary to integrate traditional Chinese medicine with Western medicine, traditional media with new media, also authoritative experts and living example.