Objective To observe the clinical effect of GSS pedicle screw system and interbody autogenous iliac bone graft fusion in the treatment of lumbar spondylolisthesis.Methods Twenty-one lumbar spondylolisthesis patients was treated by GSS pedicle screw system and interbody autogenous iliac bone graft fusion.Followed up and analyzed the clinical therapeutic efficacy,rate of detachment and reduction,interbody fusion and intervertebral height change.Results Postoperative rate of detachment and reduction had significant difference compared with preoperative [(6.41 ± 6.90)％ vs.(36.75 ± 7.11)％,(86.75 ± 24.40)％ vs.0](q =19.60,19.72,P＜ 0.01).Final follow-up rate of detachment and reduction had no significant difference compared with postoperative (q =0.70,0.96,P ＞ 0.05).Postoperative intervertebral height had significant difference compared with preoperative[L4-5:(7.6 ± 1.3) mm vs.(5.2 ± 0.8) mm; L5S1:(8.4 ± 2.2) mm vs.(6.5 ± 1.5) mm] (q =6.64,3.83,P ＜ 0.01).Final follow-up intervertebral height had no significant difference compared with postoperative (q =2.48,2.42,P ＞ 0.05).All patients were bony fusion,rate of fusion was 100.00％(21/21).In accordance with the Japan institute of orthopaedics (JOA) low back pain surgery standards,the scores of 6 months,1 year,2 years significant increased compared with preoperative [(20.50 ± 3.83),(23.58 ± 3.60),(24.91 ± 2.90) scores vs.(9.67 ± 4.45) scores] (F =71.92,q =13.28,17.06,18.69,P ＜ 0.01).No complication of internal fixation for fracture or looseness.Conclusions The clinical effect of GSS pedicle screw system and interbody autogenous iliac bone graft fusion in the treatment of lumbar spondylolisthesis is good.It has advantages of bone graft area and bone mass,rate of fusion.It is one of better methods in the treatment of lumbar spondylolisthesis.

BACKGROUND: To enhance peripheral nerve regeneration, the focus on the development of nerve conduits has been shifted from conventional conduits without internal structure to novel conduits with internal stuucture.Therefore, a comprehensive understanding of the internal structure of nerve conduits is necessary.OBJECTIVE: To review the application of tissue-engineered nerve conduits with internal structure in nerve regeneration.METHODS: A computer-based research of CNKI, WanFang, SinoMed and PubMed databases was performed to retrieve relevant literatures about the tissue-engineered nerve conduits with internal structure published from January 2009 to December 2015, using the keywords of nerve conduit/conduits/scaffolds/scaffold/channel/channels, internal scaffolds/scaffold, nerve regeneration/repair, hydrogel, acellularized/decellularized nerve in Chinese and English,respectively. Forty-seven eligible literatures were included for overview.RESULTS AND CONCLUSION: The novel nerve conduits with internal structure have many advantages over conventional conduits without internal structure, which can support, guide, promote peripheral nerve regeneration and functional recovery, especially, when modified with seed cells, neurotrophic factors or extracellular matrix, can achieve more effective results comparable to autologous nerve grafts.

OBJECTIVE:To study the effects of Acivicin or/and cisplatin on the apoptpsis of HepG2 and expression of Bcl-2,c-myc and p53.METHODS:Acivicin and cisplatin were used to treat cultured HepG2 cell line separately or in combination.The cytotoxicity was measured by MTT assay,the apoptosis by TUNEL assay and relevant regulator genes by immunohistochemical SP method.RESULTS:Acivicin in concentrations of 0.28,0.56,0.84,1.12 and 1.40mmol/L,the apoptosis rates of HepG2 were (3?1.25)%,(3.7?2.0)%,(10?1.25)%,(17.4?4.5)%and (16.9?3.5)%respectively.Acivicin in concentration of 1.4mmol/L,the apoptosis rates at 24,48 and 72h were(16.9?3.5)%,(27.9?4.3)%and (47.2?3.0)%respectively,which was significantly different from control group.The apoptosis rate of HepG2 induced by cisplatin(67?mol/L)was (73.4?1.5)%at 24h.The apoptosis rate of HepG2 induced by Acivicin(1.4mmol/L)plus cisplatin(67?mol/L)was(94.7?0.5)%at 24h,which was markedly higher than those induced by Acivicin or cisplatin alone.The expressions of Bcl-2,c-myc and p53 in all groups were increased comparing with those in control group.CONCLUSION:This study indicates that the apoptosis of HepG2 induced by Acivicin was in a dose-dependent and time-dependent manner.When Acivicin and CDDP used simultaneously,the effect of CDDP on HepG2 apoptosis was enhanced greatly.

OBJECTIVE:To cultivate qualified prescription-checking pharmacists and to ensure patients'rational drug use. METHODS:The status quo of prescription-checking pharmacists in China was analyzed and some concrete suggestions on how to cultivate qualified prescription-checking pharmacists were made.RESULTS:The phenomena of low educational level and low professional title,lack of professional knowledge and medical knowledge,lack of staff members were widespread in today's Chinese prescription-checking pharmacist professionals.CONCLUSION:The rational drug use can only be realized through being fully aware of the importance of prescription-checking pharmacists,enhancing whose admittance qualifications,working out professional standards for whom,clearly defining their responsibilities,strengthening their cultivation and increasing their numbers.

The viscosity of mucopolysaccharides in Holothuria scabra Jaeger was related to temperature, time, concentration of mucopolysaccharides and H 2O 2 during the decoloration, which was found through the experiment, in that the rise of temperature, elongation of time, decrease of concentration of Mucopolysaccharides and increase of that of H 2O 2 all lead to the decrease of the viscosity. Within a certain limit, the activity of mucopolysaccharides rises with the viscosity decrease in the experiment of acute cerebral ischemia in mice. So that it is important controlling the craft in the mucopolysaccharides production.

Suerm monoamine oxidasa isoenzymes (MAOi) were determined in 105 cases of liver disea ses and 49 normal controls.MAOi were separated into 3 bands (I. Ⅱ.Ⅲ) by polyacrylamide gel elcctrophoresis. The results revealed that MAOi Ⅲ% was increased in chronic liver diseases, especially in liver cirrhosis. Our findings suggest that serum MAOi determination is more sensitive than serum albumin, MAO, ChE, and ADA determinationsin in the early diagnosis of liver cirrhosis

Humans ; Lymphoma ; Tumor Microenvironment

Gastrointestinal stromal tumor(GIST) ,a kind of rare tumor,takes 1%～3% of all tumors in digestive tract,but are the most common in mesenehymal neoplasm.At present,the primal treatment of GIST is surgical operation,standard and rationality of surgery is the significant fatter that affects its curative effect.While imatinib is the main therapeutic tool for GIST that can' t benn resected or recurrence after operation.There is no definite circumscription between optimum and malignancy,there are many factors that influence the prognosis of GIST.We commonly apply Fletcher grading to evaluate the risk of GIST.

Adolescent ; Age Factors ; Biopsy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Kidney ; diagnostic imaging ; pathology ; physiopathology ; Kidney Failure, Chronic ; etiology ; pathology ; Male ; Multicystic Dysplastic Kidney ; complications ; Nephritis ; complications ; Nephrotic Syndrome ; complications ; Radiography

Objective To introduce direct digital dual energy subtraction radiography and explore it's clinical application.Methods The cases acquired separately by direct digital conventional radiography and direct digital dual energy subtraction radiography were compared.Results The detection rate,sensitivity and accuracy of calcifications in lungs,pulmonary nodules,stones in urinary system,calcifications of coronary artery and cardiovalvular were improved by using direct digital dual energy subtraction radiography.Conclusion Direct digital dual energy subtraction radiography is more valuable in clinical applications.