Objective To observe the clinical effect of GSS pedicle screw system and interbody autogenous iliac bone graft fusion in the treatment of lumbar spondylolisthesis.Methods Twenty-one lumbar spondylolisthesis patients was treated by GSS pedicle screw system and interbody autogenous iliac bone graft fusion.Followed up and analyzed the clinical therapeutic efficacy,rate of detachment and reduction,interbody fusion and intervertebral height change.Results Postoperative rate of detachment and reduction had significant difference compared with preoperative [(6.41 ± 6.90)％ vs.(36.75 ± 7.11)％,(86.75 ± 24.40)％ vs.0](q =19.60,19.72,P＜ 0.01).Final follow-up rate of detachment and reduction had no significant difference compared with postoperative (q =0.70,0.96,P ＞ 0.05).Postoperative intervertebral height had significant difference compared with preoperative[L4-5:(7.6 ± 1.3) mm vs.(5.2 ± 0.8) mm; L5S1:(8.4 ± 2.2) mm vs.(6.5 ± 1.5) mm] (q =6.64,3.83,P ＜ 0.01).Final follow-up intervertebral height had no significant difference compared with postoperative (q =2.48,2.42,P ＞ 0.05).All patients were bony fusion,rate of fusion was 100.00％(21/21).In accordance with the Japan institute of orthopaedics (JOA) low back pain surgery standards,the scores of 6 months,1 year,2 years significant increased compared with preoperative [(20.50 ± 3.83),(23.58 ± 3.60),(24.91 ± 2.90) scores vs.(9.67 ± 4.45) scores] (F =71.92,q =13.28,17.06,18.69,P ＜ 0.01).No complication of internal fixation for fracture or looseness.Conclusions The clinical effect of GSS pedicle screw system and interbody autogenous iliac bone graft fusion in the treatment of lumbar spondylolisthesis is good.It has advantages of bone graft area and bone mass,rate of fusion.It is one of better methods in the treatment of lumbar spondylolisthesis.

Humans ; Lymphoma ; Tumor Microenvironment

Adolescent ; Age Factors ; Biopsy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Kidney ; diagnostic imaging ; pathology ; physiopathology ; Kidney Failure, Chronic ; etiology ; pathology ; Male ; Multicystic Dysplastic Kidney ; complications ; Nephritis ; complications ; Nephrotic Syndrome ; complications ; Radiography

Objective To assess radiation dose and image quality of chest CT examinations that acquired with Flash scan technique combined with iterative reconstruction.Methods Thirty continuous patients required no-contrast chest CT with Flash scan mode and contrast enhanced chest CT with conventional CT scan mode.For each examination,the images were reconstructed by filter back projection (FBP) and sinogram affirmed iterative reconstruction (SAFIRE).The volume CT dose index (CTDIvol),dose-length product (DLP),and effective dose (ED) were estimated.The image noise,signal to noise ratio (SNR),overall subjective image quality,and respiratory motion artifacts were evaluated blindly by two radiologists.Results For Flash scan and conventional scan,SAFIRE reduced the image noise 35％ and 16％,respectively;and increased SNR 21.9％ and 25％ compared to FBP,respectively (P ＜0.05).There was significant lower subjective image scores acquired by SAFIRE than by FBP.Flash scan reduced the CTDIvol (mGy) 40.9％,DLP 32.1％,and ED 26.9％ compared to conventional CT scan mode.The motion artifact scores were reduced significantly with Flash scan mode compared to conventional scan mode.Conclusions Flash scan technique of the chest can obtain image with minimum respiratory motion artifact due to dramatically increased scan speed,and when SAFIRE was combined with,it reduces significantly radiation dose and image noise.

Objective To observe the clinical efficacy of acupuncture plus Chinese medication in treating postcholecystectomy syndrome (PCS).Method A hundred PCS patients were randomized into a treatment group of 52 cases and a control group of 48 cases. The treatment group was intervened by acupuncture at the back-Shu points and lower He-sea points plus Chinese medication, while the control group was by medication. The clinical efficacies were compared between the two groups.Result The total effective rate was 98.1% in the treatment group versus 85.4% in the control group, and the difference was statistically significant (P<0.05).Conclusion Acupuncture plus Chinese medication is an effective method in treating PCS.

Objective]To summarize professor SHEN Hong’s clinical experience in treating ulcerative colitis with enema prescription.[Method] By following the teacher clinic and sorting out the related cases, the author summarizes professor SHEN Hong’s academic experience of treatment of ulcerative colitis with enema prescription, and for proven cases. [Result]On the base of disease stage, Professor SHEN Hong uses the advantages of external therapy of Chinese medicine, and combines the syndrome differentiation and disease differentiation by the reference to the treatment of abscess,carbuncle. On the acute stage, he uses the elimination method to relieve heat and toxic material, cool blood and eliminate carbuncle. On the remission stage, he uses reinforcing method to support sore and drainage of pus and heal wound and promote tissue regeneration in case of recrudescence. Meantime, he focuses on the different locations of lesions by reasonable enema ways. The medication he uses is concise and the prescription changes flexibly with permitted addition and subtraction. The clinical curative effect is satisfactory. [Conclusion] Professor SHEN Hong’s experience is effective and worthy of inheritance and promotion.

Objective]To study the discipline of Guizhi Tang in clinical application, understand further indication of Guizhi Tang to improve clinical efficacy. [Method]After consulting medical cases about Guizhi Tang that are from different medical practitioners, analysis on the discipline of Guizhi Tang in clinical application with the help of six meridian syndrome differentiation, zang-fu differentiation and drug efficacy of Guizhi Tang. [Result]Guizhi Tang can be used to cure urticaria, coronary heart disease, nape carbuncle, high fever, half sweating and diarrhea. The cases of urticaria, diarrhea and nape carbuncle all contain symptoms of tai yang disease.The case of high fever is due to discord of ying-wei. The case of half sweating is due to discord of yin-yang. The case of coronary heart disease reflects Guizhi Tang can regulate spleen-stomach. [Conclusion] The medical cases about Guizhi Tang involve the departments of internal medicine, surgery, gynaecology and pediatrics,such as urticaria, coronary heart disease and so on. Using Guizhi Tang should get help from six meridian syndrome differentiation or zang-fu differentiation. When using six meridian syndrome differentiation, doctors should pay attention to difference of tai yang disease and other five meridian diseases. When using zang-fu differentiation, doctors should attach importance to the feature of Guizhi Tang that involves regulating yin-yang, spleen-stomach and ying-wei.

Objective:To observe the clinical efficacy of Shanghai-style tuina method based on syndrome differentiation in treating infantile diarrhea. Methods:Sixty patients were randomly divided into an observation group and a control group, 30 cases in each group. The patients in the observation group were treated by Shanghai-style tuina method based on syndrome differentiation, while the patients in the control group were treated by conventional Shanghai-style tuina method, once per day, continuous 5 d for a treatment course. The efficacy was evaluated according to the score of the quantization table for symptoms differentiation in infantile diarrhea after one course. Results:During the treatment, two cases in the control group dropped out. After treatment, the scores were significantly decreased in both groups and the intra-group differences were statistically significant (both P<0.01), indicating the two treatment methods were effective with infantile diarrhea. In the efficacies of traditional Chinese medicine (TCM) syndrome, the cured and markedly effective rate in the observation group was significantly higher than that in the control group (P<0.05), suggesting that Shanghai-style tuina method based on syndrome differentiation was more effective than conventional Shanghai-style tuina method. Conclusion:Conventional Shanghai-style tuina method is characterized by fixed acupoints, remembering easily and operating conveniently. It has a significant effect in treating children with diarrhea, and is worthy of clinical promotion and popularization. However, Shanghai-style tuina method based on syndromes differentiation requires the solid theoretical foundation and rich clinical experience of TCM. And because Shanghai-style tuina method based on syndromes differentiation is applied under the guidance of syndrome differentiation and TCM theory, it can improve the clinical efficacy.

Objective To investigate the effect of extracellular signal-regulated kinase(ERK)pathway OH nitriC oxide(NO)release by human umbilical vein endothelial cell(HUVEC)induced by placental growth factor-1(PLGF-1).Methods During January to April 2006,50 samples of umbilical vein blood were collected from newborns delivered by cesarean section due to intrauterine distress and abnormal fetal position.HUVEC were primarily cultured by trypsin digestion.Then the following procedures were performed:(1)Cells were identified using the morphology andⅧfactor immunohistochemistry methods if the culture WaS satisfactory.(2)Cells were collected,and fms.1ike tyrosin kinase(Fit-1)protein and its mRNA expression were detected with immunoprints and RT-PCR methods.(3)The protein wag extractedafter cells were treated with PLGF-1(cells were collected before the treatment and 2.5,5.10,20 min after the treatment).The protein levels of ERK were determined by immunoprints.(4)The cells were cultured witll serum-free culture medim containing PLGF-1 only(culture media were collected 20,40,160,360.480.720 and 1440 rain after the treatment).The quantity of NO was detected with nitrate reductase metllod(5)The ceHs were cultured with serum.free culture medium containing PI)98059,the inhibitor of mitogen-activated protein kinase(MAPK)/MEK for 60 min Then the cells were cultured continuously with the serum-free culture medium containing PLGF-1 for 60 mira The culture media were coilected.The quantity of NO was detected by nitrate reductase method.The samples were divided into treatment group and control group.Control group was exactly the satne in treatment time,culture condition,and time to colleet the cells as the treatment group.except that it WaS not treated with PLGF-l or PD 98059.Resuits (1)By morphology and Ⅷ factor inununohistochemistry the cultured cells were identified to be HUVEC.(2)Fit-1mRNA and protein were expressed in HUVEC.(3)Expression of ERK protein started to increaSe at 2.5 min after treatment of HUVEC with PLGF-1,reaching the peak at 5 min,and decreased at 10 min.(4)Incomparison with the control group.NO started to increase at 20 rain after treatment of HUVEC with PLGF-lat 480 min(15.82±0.69)μmol/L Comparison between the two groups showed a significant difference (P<0.05).(5)ReleaSe of NO from the cells treated with PD98059 for 1 hour and PLGF WaS significantly inhibited,compared with the ceils treated with PLGF-1 only.Conclusion ERK pathways play an important role in N0 release bv HUVEC induced by PLGF.

Breast cancer ranks among the most prevalent malignancies in women. Specific aspects of both breast cancer cells and the bone microenvironment contribute to the development of bone metastases. Breast cancers express chemokine receptors, integrins, cadherins, and bone-resorbing and bone-forming factors that contribute to the successful and preferential spread of tumor from breast to bone. Bone is rich in growth factors and cell types that make it a favorable environment for breast cancer cell growth. Once breast cancer cells enter the bone, breast cancer cells can secrete factors that act on bone cells and other cells within the bone, causing them to secrete factors that act on adjacent cancer cells. The steps in the metastatic cascade and the vicious cycle within bone offer unique targets for adjuvant treatments to treat and cure bone metastasis.