1.Expression of rat protamine gene in MEL cells.
Yu CHEN ; Ren-ren FEI ; Xiao-fang WU ; Ye-hua GE ; Hui CHEN ; Jing MA ; Dai-shu HAN ; She-pu XUE
Acta Academiae Medicinae Sinicae 2002;24(6):601-605
OBJECTIVETo investigate the expression of rat protamine (RP) gene in MEL cells and the effect on cell growth.
METHODSEukaryotic expression plasmid pCR-3.1-RP was constructed and transfected into MEL cells. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.
RESULTSTransfected MEL cells showed lower growth rate, mitotic index and colony-forming rate. Volumes of cells were reduced and reduction of RNA transcription was observed.
CONCLUSIONThese results suggest that expression of RP in MEL cell may inhibit the cell growth and proliferation.
Animals ; Cell Division ; Leukemia, Erythroblastic, Acute ; genetics ; pathology ; Plasmids ; Protamines ; genetics ; metabolism ; Rats ; Transfection ; Tumor Cells, Cultured
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