Objective? To explore the knowledge-attitude-practice on patients identification among nurses in hospitals at different levels so as to provide a basis for understanding the knowledge-attitude-practice on patients identification among nurses in hospitals at different levels. Methods? From January 2017 to July 2017, a total of 700 nurses at Class Ⅲ, Ⅱ and Ⅰ hospitals in Shenzhen were investigated with the questionnaire with the methods of group sampling, probability proportionate and cluster sampling. The self-designed Knowledge-attitude-practice on Patients Identification Questionnaire of Nurses was used to investigate the knowledge-attitude-practice on patients identification among nurses. Results? A total of 700 questionnaires were sent out and 658 of them were collected with 94.0% for the valid recovery rate. The total scores of knowledge-attitude-practice on patients identification of nurses at ClassⅢ, Ⅱ and Ⅰ hospitals were (124.82±4.65), (117.75±3.85) and (116.58±3.96) respectively. The score of knowledge-attitude-practice on patients identification of nurses at Class Ⅲ hospitals was the highest followed by that at Class Ⅱ hospitals, and that at Class Ⅱ hospitals was the lowest. There were statistical differences in the scores of knowledge and practice on patients identification of nurses at different levels of hospitals (P＜0.05), and there was no statistical difference in the score of attitude (P＞ 0.05). There were statistical differences in the scores of knowledge-attitude-practice on patients identification among nurses with different educational background and positional titles in hospitals at different levels (P＜0.05), and there were no statistical differences in the scores of knowledge-attitude-practice on patients identification among nurses with different sexes and ages (P＞ 0.05). Conclusions? As a whole, the knowledge-attitude-practice on patients identification among nurses at Class Ⅲ hospitals is excellent. However, we need to improve the knowledge-attitude-practice on patients identification among nurses, especially with nurse in the positional title and with junior college and bellow in the educational background, at Class Ⅰ and Ⅱ hospitals.