Aims: Quantitative screening of phytochemical properties and antimicrobial activities were done on some plants of
importance in human medicine as traditional herbs to treat fish diseases in Malaysia. Six extracts of Vitex trifolia, Aloe
vera, Strobilanthes crispus, Clinacanthus nutans, Pereskia grandifolia and Peperomia pellucida were determined for
phytochemical properties and their antibacterial activities against common freshwater pathogens i.e. Streptococcus
agalactiae, Aeromonas hydrophila and Enterobacter cloacae.
Methodology and results: Qualitative screening of phytochemical properties in herbs were determined using
conversional method for flavonoids, tannins, saponin, alkaloids steroid and glycoside The results showed flavonoid was
presence in all plant extracts. For the antimicrobial activity, the aqueous and methanolic extracts were tested by using
disk diffusion method. Antimicrobial assay of methanolic crude extracts (25 to 100 mg/mL) showed effectiveness against
the pathogenic bacteria. Comparatively, all aqueous extracts did not show any antimicrobial activity. Strong antibacterial
activity was shown by the methanolic extracts of V. trifolia, A. vera and S. crispus while moderate antimicrobial activity
was shown by C. nutans, P. grandifolia and P. pellucida.
Conclusion, significance and impact study: The current results indicated that the studied plants might indeed be
potential sources of natural antimicrobial agents to control fish diseases.
Plants, Medicinal