Objective To explore the clinical characteristics of infectious mononucleosis (IM)with Epstein-Barr virus (EBV)and mycoplasma pneumoniae (MP)infection in children.Methods Children with IMwho were all positive for EBV and hospitalized in our department from January 2008 to July 2015 were included and divided into the EBV +MP group and the EBV group according to the results of MP.The manifestations,laboratory variables and outcomes were compared in the two groups.Results Of all these 61 cases,18 children (29.51%)were accompanied with EBV and MP infection.The age of the EBV +MP group was older than the EBV group [(4.44 ±2.75)vs (2.90 ± 2.08)years,t =2.401,P =0.02].Moderate to severe enlarged tonsils,hepatomegaly and complications (especially cough and gastrointestinal symptoms)were more common in the EBV +MP group than the EBV group with significant differences (remarkable tonsillitis:88.89 vs 48.84%,hepatomegaly:55.56 vs 13.95%,complications:72.22 vs 44.19%;χ2 =8.529,10.719 and 3.999 respectively;P =0.003,0.001 and 0.046 respectively).The WBC and lymphocyte counts,percentage of abnormal lymphocyte and the levels of glutamyltranspeptidase in the EBV +MP group were also significantly higher than the EBV group [WBC counts:(18.17 ±7.17)×109 /L vs (13.70 ±7.12)×109 /L], lymphocyte counts:(11.61 ±6.04)×109 /L vs (7.65 ±4.82)×109 /L,abnormal lymphocyte proportion:(20.69 ± 13.03)% vs (13.00 ±11.20)%,serum glutamyltranspeptidase:(99.41 ±91.20)U /L vs (47.95 ±69.22)U /L;t =2.231,2.716,2.215 and 2.239 respectively;P =0.029,0.009,0.031 and 0.029 respectively).But the average hospital stay and the recovery time of manifestations showed no significant differences in the two groups (P >0.05). Conclusion IMchildren with EBV and MP infection have more cases with moderate to severe enlarged tonsils,hepa-tomegaly and complications,moreover present higher lymphocyte and similar outcomes.MP should be tested in all IM children.The early diagnosis and treatment are the keys to improve the prognosis of IM children with EBV and MP infection.

OBJECTIVE: To reveal the characteristics of gene expression in adrenal gland of H22 tumor mice with typical syndromes and in different liver cancer stages. METHODS: By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, we observed adrenal gland gene expression in H22 tumor mice with pathogenic factor-toxin predominance syndrome and qi deficiency syndrome in the earlier stage, yang-qi deficiency syndrome in the intermediate stage, and qi-yin-yang deficiency syndrome in the advanced stage. Genes highly expressed and remarkably different were analyzed in this study. RESULTS: A total of seventy-three up-regulated coincident genes and twenty-six down-regulated coincident genes in different stages were investigated in the study. Up-regulated coincident genes included Hp, C3, Anxa1, Procr, C2, Il4ra, Cd14, Ptprc, Cd52, C4b, Eno3, Xdh, Gpx3, and so on. Down-regulated coincident genes included nervous system function-related genes such as Plp1, Mbp, Aldh1a1, Cck, Atn1, genes associated with electrolyte metabolism such as Aldh1a1 and Slc22a17, genes related to signal transduction such as Cxcr4, Spag5 and Stmn3, etc, and genes related to transcriptional control and protein biosynthesis such as Hspa1a, Dnajb1, Thra, Hhex and so on. CONCLUSION: With the development of the tumorigenesis, the symptoms and signs and differentially expressed genes in adrenal gland of H22 tumor mice can be measured. Up-regulated and down-regulated coincident genes may be the features of H22 tumor mice different from those of normal mice.